JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Mingliang Zhang,Weihao Zhu,Hongfei Liu,Kaihua Pan,Qian Li,Qian Zhu,Yanni Huang,Changchang Wang,Junqiang Hu,Mingli Jiang,Xin Yan,Qing Hong
Acetaminophen (APAP), an analgesic and antipyretic drug, is commonly detected in wastewater treatment plant (WWTP) effluents, surface water, and soil, indicating its status as an emerging environmental contaminant. In this study, we isolated a bacterium, Pseudomonas taiwanensis AP-1, capable of completely mineralizing APAP and utilizing it as the sole carbon source for growth. A newly identified metabolite, γ-glutamyl-4-aminophenol (γ-G4AP), was reported for the first time in the degradation of APAP by strain AP-1. Two amidases (ApaH1 and ApaH2), responsible for the conversion of APAP to 4-aminophenol (4-AP), were identified through a combination of genomic comparison, heterologous expression, and gene knockout. Notably, ApaH1 played a pivotal role in the degradation of APAP by strain AP-1. The catalytic triad of ApaH1 (K82-S161-S185) and ApaH2 (K85-S160-S184) were identified as by molecular docking and site-directed mutagenesis. Additionally, a gene cluster apd for the metabolism of 4-AP was also successfully identified in strain AP-1, consisting of the aniline dioxygenase gene cluster apdBCD1D2EF and the BT catabolic gene apdGH . Interestingly, the 4-AP metabolic gene cluster apd was highly conserved among other Pseudomonas strains capable of APAP degradation. Our results provide new insights into the mechanism of APAP biodegradation and strain AP-1 may be a promising bacterium for the bioremediation of APAP pollutions.
研究领域:
环境科学
·
微生物学
·
药物代谢
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产品关联
DOI
PubMed
高影响因子
Microbiome
·
2025
·
IF 12.7
Chao-Fan Yin,Piaopiao Pan,Tao Li,Xin Song,Ying Xu,Ning-Yi Zhou
Background Acetaminophen, a widely used analgesic and antipyretic drug, has become a significant aquatic micro-pollutant due to its extensive global production and increased consumption, particularly during the COVID-19 pandemic. Its high-water solubility leads to its pervasive presence in wastewater treatment plants (WWTPs), posing substantial risks to the environment and human health. Biological treatment is one of the promising approaches to remove such pollutants. Although previous studies have isolated acetaminophen-degrading pure cultures and proposed catabolic pathways, the interactions between microbiotas and acetaminophen, the distribution feature of acetaminophen degradation genes, and the gene-driven fate of acetaminophen in the real-world environment remain largely unexplored. Results Among the water samples from 20 WWTPs across China, acetaminophen was detected from 19 samples at concentrations ranging from 0.06 to 29.20 nM. However, p -aminophenol, a more toxic metabolite, was detected in all samples at significantly higher concentrations (23.93 to 108.68 nM), indicating the presence of a catabolic bottleneck in WWTPs. Metagenomic analysis from both the above 20 samples and global datasets revealed a consistently higher abundance of initial acetaminophen amidases compared to downstream enzymes, potentially having explained the reason for the bottleneck. Meanwhile, a close correlation between initial amidases and Actinomycetota revealed by genome-based taxonomy suggests a species-dependent degradation pattern. Additionally, a distinct amidase ApaA was characterized by newly isolated Rhodococcus sp. NyZ502 (Actinomycetota), represents a predominant category of amidase in WWTPs. Significant phylogenetic and structural diversity observed among putative amidases suggest versatile acetaminophen hydrolysis potential in WWTPs. Conclusions This study enhances our understanding of acetaminophen’s environmental fate and highlights the possible occurrence of ecological risks driven by imbalanced genes in the process of acetaminophen degradation in global WWTPs. Your browser is not supporting the HTML5
研究领域:
环境科学
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微生物学
·
基因组学
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2023
·Vol. 35
·期 9
·e202300064
·
IF 3
Jinjin Zhang,Wenjing Liu,Qi Wang,Jianying Qu
In this work, two-dimensional cobalt metal organic framework nanosheets ( CoTP ) were prepared by the simple hydrothermal reaction of cobalt nitrate hexahydrate with terephthalic acid, and then calcined at a high temperature under the environment of nitrogen to obtain cobalt carbon nanomaterials ( Co-CNs ). The gold nanomaterials ( AuNPs ) were prepared by redox reaction. The prepared materials were characterized and analyzed by XRD, SEM and IR. A novel electrochemical sensor ( AuNPs/Co-CNs ) was constructed successfully on a glassy carbon electrode combining Co-CNs with AuNPs , which showed significant electrocatalytic activities for acetaminophen ( PA ) and 4-aminophenol ( 4-AP ) and could be used to detect them simultaneously. Under the optimal conditions, the linear ranges for determination of PA and 4-AP were 0.50–250.00 μM and 0.50–350.00 μM with the detection limits of 0.11 μM and 0.10 μM, respectively. Compared with other related reports, the proposed sensor exhibits a wider linear ranges, good sensitivity, accuracy and selectivity with potential applications in real sample analysis.
研究领域:
分析化学
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材料科学
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医学检测
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DOI
高影响因子
ELECTROANALYSIS
·
2025
·Vol. 37
·期 5
·e12050
·
IF 2.3
Sumin Xu,Wenjing Liu,Jinjin Zhang,Jianying Qu
In this work, Cu nanosphere-anchored Cu-based MOF derivants were synthesized by high-temperature calcination using Cu(BDC) as precursors and then combined with gold nanoparticles to give AuNPs/Cu/C composites. The proposed materials were characterized by SEM, XRD, XPS, FTIR, and EIS. Based on these composites, a sensor was constructed, which showed good electrocatalytic activities to the redox of acetaminophen (PA) and P-aminophenol (4-AP) and could be used to detect them simultaneously. Under optimal experimental conditions, the linear ranges for detection of PA and 4-AP were 0.50–150.00 and 0.50–120.00 μM with the detection limits of 0.056 and 0.084 μM, respectively. Thanks to the hierarchical and porous structural characteristics of the Cu/C material and its synergistic effect with AuNPs, it shows good sensing performance in the simultaneous detection of PA and 4-AP. The sensor has wider linear ranges, lower detection limits, good selectivities, and higher accuracies with potential environmental detection application prospects.
研究领域:
分析化学
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2011
·Vol. 175
·期 1
·39-46
·
IF 2.578
Yin Huanshun,Shang Kun,Meng Xiaomeng,Ai Shiyun
A differential pulse voltammetric method was developed for the simultaneous determination of paracetamol, 4-aminophenol and dopamine at pH 7.0 using a glassy carbon electrode (GCE) coated with gold nanoparticles (AuNPs) and a layered double hydroxide sodium modified with dodecyl sulfate (SDS-LDH). The modified electrode displays excellent redox activity towards paracetamol, and the redox current is increased (and the corresponding over-potential decreased) compared to those of the bare GCE, the AuNPs-modified GCE, and the SDS-LDH-modified GCE. The modified electrode enables the determination of paracetamol in the concentration range from 0.5 to 400 μM, with a detection limit of 0.13 μM (at an S/N of 3). The sensor was successfully applied to the stimultaneous determination of paracetamol and dopamine, and of paracetamol and 4-aminophenol, respectively, in pharmaceutical tablets and in spiked human serum samples.
1. Gold nanoparticles and organophillic layered double hydroxide modified glassy carbon electrode was fabricated. 2. The modified electrode displayed excellent redox activity towards paracetamol. 3. This electrode was successfully applied to the simultaneous determination of paracetamol and dopamine, and of paracetamol and 4-aminophenol, respectively
研究领域:
分析化学
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生物传感器
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药物分析
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电化学检测
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2020
·Vol. 167
·期 6
·066512
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IF 3.721
Nannan Dou,Jianying Qu
A novel highly sensitive nanoelectrochemical sensor was prepared using graphene oxide/titanium dioxide/gold nanoparticles composites (GT@AuNPs) modified glassy carbon electrode (GCE) to measure paracetamol (PA) and 4-aminophenol (4-AP) simultaneously. It can be found that PA and 4-AP could be completely separated on the GT@AuNPs/GCE using cyclic voltammetry and differential pulse voltammetry technique. Under the best conditions, the linear responses for PA from 0.10 to 300.00 μM with the detection limits of 0.78 nM. And the linear responses for 4-AP from 0.10 to 150.00 μM with the detection limits of 2.36 nM. This sensor can be used to detect acetaminophen in pharmaceutical formulations with satisfactory results, indicating that the electrochemical sensor has potential application value.
研究领域:
分析化学
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材料科学
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药物分析
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2011
·Vol. 16
·期 4
·1635-1642
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IF 2.234
Ye Daixin,Xu Yanhong,Luo Liqiang,Ding Yaping,Wang Yulong,Liu Xiaojuan
A novel electrochemical sensor based on LaNi 0.5 Ti 0.5 O 3 /CoFe 2 O 4 nanoparticle-modified electrode (LNT–CFO/GCE) for sensitive determination of paracetamol (PAR) was presented. Experimental conditions such as the concentration of LNT–CFO, pH value, and applied potential were investigated. Under the optimum conditions, the electrochemical performances of LNT–CFO/GCE have been researched on the oxidation of PAR. The electrochemical behaviors of PAR on LNT–CFO/GCE were investigated by cyclic voltammetry. The results showed that LNT–CFO/GCE exhibited excellent promotion to the oxidation of PAR. The over-potential of PAR decreased significantly on the modified electrode compared with that on bare GCE. Furthermore, the sensor exhibits good reproducibility, stability, and selectivity in PAR determination. Linear response was obtained in the range of 0.5 to 901 μM with a detection limit of 0.19 μM for PAR.
研究领域:
生物医学工程
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药物分析
·
电化学检测
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DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 19
·2223-2228
·
IF 2.896
Bo-Wen Liu,Peng-Cheng Huang,Fang-Ying Wu
A novel and efficient light-controlled colorimetric assay for the quantification and detection of nitroreductase (NTR) was constructed based on p-aminophenol (pAP)-catalyzed and nicotinamide adenine dinucleotide (NADH)-mediated generation of AgNPs. Due to the hydrolysis of p-nitrophenol by NTR in the presence of NADH, the hydrolysis product can be used as a catalyst to catalyze the reduction of Ag+ by NADH under the light. As the concentration of NTR increases, the value of absorbance at ca. 400 nm (A400) decreases and the color of the solution turns from brown to bright yellow. A linear correlation was obtained between A400 and the NTR concentration in the range from 1–50 μg mL−1 and the limit of detection (LOD) is 0.27 μg mL−1. The detection system does not respond to other common biological molecules due to the specificity of enzymes and the effect of the nitroreductase inhibitor on the NTR activity was also tested. Finally, we applied the assay to determine NTR in human serum samples by spiking different concentrations of NTR with a recovery of 85.2%–92.5%.
研究领域:
生物化学
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医学检测
·
纳米技术
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DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 15
·3000-3005
·
IF 1.915
Hanzhi Zhang,Lu Zhang,Yu Ding,Wenqing Zhang,Xin Zhang,Yehua Shen,Fengchun Yang
A new method for determination of glutathione (GSH) was carried out with acetaminophen (AAP) mediation on a NiPd nanoparticle modified electrode. The as-prepared sensor exhibited excellent electrocatalytic activity for determining GSH with a good linear correlation with GSH concentration in a broad range from 0.5 to 3000 μM, a high detection sensitivity (481.69 μA mM−1 cm−2), and a low detection limit of 0.5 μM. The mechanism for GSH oxidation was also investigated by cyclic voltammetry and linear sweep voltammetry. In addition, the proposed sensor was also successfully employed to detect GSH in real samples.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2018
·
IF 6.042
Jian Sun,Jiahui Zhao,Xingfu Bao,Qifeng Wang,Xiurong Yang
Diethanolamine (DEA) has been extensively utilized as an alkaline buffer in current assays of alkaline phosphatase (ALP) activity in the past decades. While playing the role of a buffer, the chemical reactivity of DEA has been widely ignored in such assays. Herein, we report an interesting chromogenic interaction between DEA and 4-aminophenol (AP) in the presence of H2O for the first time, which inspires us to develop a novel DEA-participated ALP activity assay by using 4-aminophenyl phosphate (APP) as a substrate. This APP/DEA-based colorimetric approach has been proved to be comparable and even superior to the conventional p-nitrophenyl phosphate (pNPP)-based one, especially in the low ALP activity region, due to its higher sensitivity. The clear response mechanism and excellent sensing performance ensure that it can be further applied to determining ALP activity in real biological samples, screening potential ALP inhibitors in vitro, establishing ALP-enabled ELISA, and even fluorophore-assisted fluorescent ALP activity assay. It is demonstrated that this strategy not only possesses a good feasibility, but also exhibits a promising outlook for a series of ALP-related and -extended detections.
研究领域:
生物化学
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医学检测
·
酶学研究
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DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 63
·40022-40032
·
IF 3.361
Yiting Lin,Xiting Mo,Yamin Zhang,Minghua Nie,Caixia Yan,Leliang Wu
Owing to the high concentration of pharmaceuticals in urine, the degradation of these organic pollutants before their environmental release is highly desired. Peroxymonosulfate (PMS) is a desirable oxidant that can be applied to environmental remediation; however, the performance and mechanism of PMS for the degradation of pharmaceuticals in the urine matrix have not been investigated. Herein, PMS was first discovered to efficiently degrade typical pharmaceuticals in hydrolyzed urine (HU) by selecting acetaminophen (ACE) as a target compound. Quenching experiments revealed that singlet oxygen (1O2) and hydroxyl radicals (HO˙) were observed in the HU/PMS system, but the principal reactive species (RS) responsible for ACE removal was 1O2. The major constituents of HU, including SO42− and organics (creatine, creatinine and hippuric acid), hardly affected the elimination of ACE, whereas Cl−, H2PO4− and NH4+ would accelerate ACE degradation. Besides, HCO3− slightly inhibited this process. The ACE degradation efficiency was enhanced using photo-irradiation, including sunlight and visible light, although increasing the reaction temperature could, interestingly, hardly accelerate the degradation rate of ACE. Three-dimensional excitation–emission matrices (3D-EEMs) have indicated that other intermediates that have a higher fluorescence intensity might be generated in the HU/PMS system. Finally, nine intermediate products were determined and the degradation pathways of ACE were proposed. Overall, the results of this study illustrated that PMS is an efficient oxidant for the degradation of ACE in HU.
研究领域:
环境科学
·
药学
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DOI
高影响因子
Biomaterials Research
·
2025
·
IF 9.6
Yangjing Su,Yunong Zeng,Minjie Zhou,Meihui Liao,Ping Qin,Rong Wu,Jiaochan Han,Xiaoqi Liang,Ze Wang,Jingjing Jiang,Zhichao Yu,Xintao Huang,Kaixin Ding,Peiheng Guo,Yi He,Ying Du,Tingting Duan,Haitao Yuan,Yuewei Ge,Ali Chen,Wei Xiao
Acetaminophen (APAP) overdose has long been recognized as the main cause of drug-induced liver injury (DILI), characterized by glutathione (GSH) depletion and reactive oxygen species (ROS) accumulation, leading to ferroptosis and inflammatory responses. There is an urgent need for liver-protective agents to combat ferroptosis, modulate oxidative stress, and ameliorate inflammation. Catechin, a well-known polyphenol compound, has been shown to have antioxidant potential. However, its protective role on APAP-induced liver injury (AILI) has not been elucidated. In this study, we evaluated the modulating effects of catechin on AILI and observed that catechin attenuated liver injury by reducing inflammation. Mechanistically, catechin alleviated hepatic oxidative stress by inhibiting ROS accumulation, malondialdehyde (MDA) production, and GSH depletion. Furthermore, catechin, as a hepatic injury reparative agent, could counteract APAP-induced hepatocyte ferroptosis by activating the xCT/GPX4 pathway, and is expected to be a novel natural inhibitor of ferroptosis. Additionally, the transcriptomic results indicated that the inhibition of Stat1 by catechin is important for the management of AILI. Inhibition of signal transducer and activator of transcription 1 (STAT1) expression, achieved through the use of the STAT1 inhibitor fludarabine in vivo and small interfering RNA (siRNA) in vitro, was confirmed to attenuate APAP-induced ferroptosis. In conclusion, the present study identified a novel natural drug inhibitor of ferroptosis and revealed its mechanism of action to inhibit ferroptosis, regulate oxidative stress, and ameliorate inflammation in AILI. This further provides new insights into the novel natural ferroptosis inhibitors for the treatment of ROS-related inflammatory diseases.
研究领域:
药理学
·
肝脏病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
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