INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2025
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IF 4.1
Qingyun Li,Shaohui Yan,Zhengfeng Jiang,Liang Xian,Jianjia Du,Haibo Liu,Youyan Liu
Cyanide and phenol are highly toxic chemicals that exert severe inhibitory effects on microorganisms, posing significant challenges for their removal by biological methods in coexistent systems. In this study, we successfully isolated a bacterial strain named JF101, identified as Alcaligenes faecalis , which was capable of degrading both cyanide and phenol. The interactions between cyanide and phenol during biodegradation were investigated. It was found that the presence of 100 mg/L cyanide significantly enhanced the degradation of phenol, leading to the complete degradation of 1000 mg/L phenol within 86 h. In contrast, only 21.9% of phenol was degraded in the absence of cyanide. Additionally, the presence of phenol had minimal impact on cyanide degradation; a concentration of 100 mg/L cyanide could be rapidly and completely degraded within 40 min even in the presence of up to 1000 mg/L phenol. Five empirical kinetic models of Haldane, Aiba, Edward, Yano, and Webb were employed to describe the degradation processes of phenol and cyanide in both single-substrate and dual-substrate systems. The putative degrading enzymes, degradation products, and pathways associated with the co-degradation of cyanide and phenol were systematically analyzed. The formamide and NH 4 + -N produced from the rapid degradation of cyanide served as nitrogen sources, facilitating the resumed growth of strain JF101 and enhancing phenol degradation.
研究领域:
环境微生物学
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污染治理
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
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IF 3.571
Hengqian Lu,Haiqin Chen,Xin Tang,Qin Yang,Hao Zhang,Yong Q. Chen,Wei Chen
The objective of the present study was to reveal the effects of four types of nitrogen sources (soymeal, yeast extract, KNO3, and ammonium tartrate) on the lipid metabolism of the oleaginous fungus Mortierella alpina using untargeted lipidomics, targeted fatty acid, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. Our results showed clear differences in the contents and compositions of lipids between four types of nitrogen sources. Soymeal and ammonium tartrate supplementation favored the accumulation of triglycerides with arachidonic acid (ARA) and C16–18 fatty acids, respectively. These results were further validated by our targeted fatty acid analysis. RT-qPCR analysis of related genes in M. alpina between the four nitrogen source conditions found that soymeal supplementation dramatically increased the expression of GPAT, ELOVL, and Δ12/Δ6 desaturase. Our findings provided new insights into the regulation of lipid biosynthesis in M. alpina and potential avenues for genetic manipulation and highlighted the importance of an optimal nitrogen source for ARA-rich oil production.
研究领域:
生物化学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Toxics
·
2023
·Vol. 11
·期 10
·803
·
IF 4.6
Chengzhi Li,Yuxi Feng,Peng Tian,Xiaozhang Yu
The accumulation of proline impacts the defense mechanisms of plants against the harmful effects of adverse environmental conditions; however, its concentration in plants is associated with the metabolism of N. Therefore, the effects of exogenous organic [glutamate (Glu)/arginine (Arg)] and inorganic [nitrate (NO3−)/ammonium (NH4+)] N on the accumulation of proline (Pro) in rice plants under trivalent chromium [Cr(III)] stress were studied through using the mass balance matrix model (MBMM). Application of ‘NH4+’ showed the largest contribution to the Pro content in rice shoots under different concentrations of Cr(III), followed by ‘NO3−’, ‘Arg’, and ‘Glu’ applications. On the other hand, ‘Arg’ application displayed the largest contribution to the Pro content in roots under Cr(III) stress, followed by ‘NH4+’, ‘Glu’, and ‘NO3−’ applications. The combined application of ‘NH4++Arg’ showed the greatest contribution to the Pro content in both roots and shoots of Cr(III)-treated rice seedlings, while the application of ‘NO3−+Glu’ showed the least contribution to the Pro content in rice seedlings. The current study indicated that the endogenous level of Pro in rice seedlings is quite sensitive to Cr(III) stress under different N sources, and the mathematical modeling showed a reliable result while estimating the relationship between Pro content and N source application.Keywords:proline;rice;trivalent chromium;nitrogen source;mass balance
研究领域:
植物生理学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2023
·
IF 4.3
Shihua Liao,Xiang Long,Yanshun Ma,Shaogui Wu,Jiayang Wang
Multicolor fluorescent carbon dots (CDs) have wide application prospects in many fields. Presented herein reported a synthesis of multicolor fluorescent CDs using zanthoxylum bungeanum as a carbon precursor. By changing the nitrogen sources, blue, green, and red fluorescent carbon dots (B-CDs, G-CDs, and R-CDs) with stable optical properties and high quantum yields (QY) were synthesized. Their emission wavelengths are 492 nm, 523 nm, and 601 nm respectively and their QYs were measured to be 13.1%, 11.2%, and 9.8%, respectively. These CDs exhibit good salt resistance, photobleaching resistance, and chemical stability. These characteristics make them suitable for various applications, particularly in the field of anti-counterfeiting fluorescent ink. This study provides an economic and efficient strategy for synthesizing multicolor fluorescent CDs derived from natural products, highlighting the potential for producing multicolor CDs and enhancing their application value.
研究领域:
材料科学
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化学
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植物学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2018
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IF 6.434
Hong-cheng Fan,Jing Yu,Rong-ping Chen,Lei Yu
A novel bioflocculant, A-GS408, produced by Klebsiella oxytoca GS-4-08 cultured in acetonitrile (ACN) as sole nitrogen source was investigated in this study. A complete degradation of 1 g l −1 of ACN was achieved in 350 h, and 4.6 g of crude A-GS408 can be obtained in one litter of synthetic medium. The as-prepared bioflocculant exhibits good flocculation efficiency (over 90%) toward Kaolin solution with the aid of Fe 3+ . Chemical analysis showed that the bioflocculant was mainly composed of polysaccharides (46.3%) and proteins (20.6%). FTIR and XPS results indicated the abundant carboxyl, amine and hydroxyl groups in A-GS408 s, which play an important role on Pd 2+ and Cu 2+ adsorption. The adsorption of heavy metals can be well stimulated by Freundlich isotherm equation, and the K f was up to 439.2 mg 1-1/n l 1/n g −1 and 112.2 mg 1-1/n l 1/n g −1 for Pb 2+ and Cu 2+ , respectively. The kinetic fitting results proved that the adsorption of heavy metals by A-GS408 was chemisorption. This study may provide a new method for preparation of bioflocculant, which can not only degrade toxic compound i . e ., acetonitrile, but also can reuse considerable nitrogen source from nitrile-containing wastewater.
研究领域:
环境生物学
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微生物学
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DOI
高影响因子
Agronomy-Basel
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2022
·Vol. 12
·期 7
·1489
·
IF 3.949
Kai Yao,Yanqing Wang,Yanyou Wu
Little is known about the competition between and niche differentiation of water and nutrients between angiosperm and gymnosperm tree species under prolonged drought stress, especially in fragile environments. We imposed 21 d drought and competition treatments onBroussonetia papyriferaandPlatycladus orientalisand measured water, N, and P contents, the isotopic composition of N and C, the activity of P assimilation enzymes, and stomatal conductance under solo planting and mixed planting to characterize resource diversity and competition in response to treatments. The N content, δ13C, δ15N, phosphomonoesterase, phosphodiesterase,gs, and foliage water content were significantly affected by the soil water content. The δ15N content in young leaves showed that N competition between these two plants could be alleviated through niche differentiation, but the changes in the PDE: PME ratio for these two plants indicated that they lost the niche differentiation of the P source under drought stress. Additionally, it was observed that foliage water content, WUE, N contents, and N and P sources were significantly affected by interspecific competition, andBroussonetia papyriferabenefited from water competition under moderate drought. Our results indicate that plants have different competition and niche differentiation modes to different nutrients under drought stress, and the effect of interspecific water competition should be seriously considered in mixed forests in semiarid areas.Keywords:angiosperm;gymnosperm;interspecific competition;water stress;stable isotopic
研究领域:
植物学
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生态学
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产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
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2024
·Vol. 38
·期 8
·e7556
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IF 3.7
Muhammad Jawad Ahmad,Xue Lu,Liye Liang,Guangming Li
Since catalytic chemoselective reduction of corresponding nitroarenes is a significant route to produce functionalized anilines, developing inexpensive and highly active catalyst is imperia. Herein, the Co-Ce-H 2 salen-hmta composite was first prepared by grinded completely the mixture of Co-H 2 Salen and hexamethylenetetramine (hmta) in 1:1 mol ratio in a mortar. The 3d–4f hetero-metal Co-Ce-H 2 salen coordination polymer acted as a support precursor and hmta provided the nitrogen source. Then, a target catalyst of Co/NC-CeO 2 -hmta were afforded by pyrolysis of Co-Ce-H 2 salen-hmta composite at 800°C in the presence of argon atmosphere. When the target catalyst of Co/NC-CeO 2 -hmta and reference catalysts were applied for the hydrogenation of nitroarenes under N 2 H 4 , the target catalyst of Co/NC-CeO 2 -hmta exhibited better performance than reference catalysts in terms of conversion, yield, selectivity, and recyclability. Spectral analysis suggested that the high catalytic performance of a target catalyst of Co/NC-CeO 2 -hmta were attributed to highly dispersed metallic Co 0 , rich oxygen vacancies, and nitrogen-doped carbon matrix.
研究领域:
化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Biomolecules
·
2019
·Vol. 9
·期 11
·695
·
IF 4.694
Dong-Sheng Wang,Xin-Jun Yu,Xiao-Yu Zhu,Zhao Wang,Hui-Juan Li,Zhi-Peng Wang
In the current study, corn steep liquor (CSL) is evaluated as an ideal raw agro-material for efficient lipid and docosahexaenoic acid DHA production byAurantiochytriumsp. Low CSL level in medium (nitrogen deficiency) stimulated the biosynthesis of lipids and DHA while inhibiting cellular growth. The transcriptomic profiles of theAurantiochytriumsp. cells are analyzed and compared when cultured under high (H group), normal (N group), and low (L group) levels of CSL in the medium. The discriminated transcriptomic profiles from the three groups indicates that changes in CSL level in medium result in a global change in transcriptome ofAurantiochytriumsp. The overall de novo assembly of cDNA sequence data generated 61,163 unigenes, and 18,129 of them were annotated in at least one database. A total of 5105 differently expressed (DE) genes were found in the N group versus the H group, with 2218 downregulated and 2887 upregulated. A total of 3625 DE genes were found in the N group versus the L group, with 1904 downregulated and 1721 upregulated. The analysis and categorization of the DE genes indicates that the regulation mechanism of CSL involved in the perception and transduction of the limited nitrogen signal, the interactions between the transcription factors (TFs) and multiple downstream genes, and the variations in downstream genes and metabolites, in sequence, are illuminated for the first time in the current study.Keywords:Aurantiochytriumsp.;nitrogen-deficiency;RNA-seq;corn steep liquor (CSL);signal transduction;DHA
研究领域:
生物技术
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分子生物学
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DOI
PubMed
高影响因子
Insects
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2021
·Vol. 12
·期 6
·507
·
IF 2.769
Yan Lu,Shouyu Zhang,Shibo Sun,Minghuo Wu,Yongming Bao,Huiyan Tong,Miaomiao Ren,Ning Jin,Jianqiang Xu,Hao Zhou,Weiping Xu
Simple SummaryBlack soldier fly larvae (BSFL) have received global research interest and industrial application due to their high performance on the organic waste treatment. However, the substrate C/N property, which may affect larvae development and the waste bioconversion process greatly, is significantly less studied. The current study focused on the food waste treatment by BSFL, compared the nitrogen supplying effects of 9 nitrogen species (i.e., NH4Cl, NaNO3, urea, uric acid, Gly, L-Glu, L-Glu:L-Asp (1:1,w/w), soybean flour, and fish meal), and further examined the C/N effects on the larval development and bioconversion process. We found that NH4Cl and NaNO3led to poor larval growth and survival, while 7 organic nitrogen species exerted no harm to the larvae. Urea was further chosen to adjust the C/Ns. Results showed that lowering the C/N from the initial 21:1 to 18:1–14:1 improved the waste reduction and larvae production performance, and C/N of 18:1–16:1 was further beneficial for the larval protein and lipid bioconversion, whereas C/N of 12:1–10:1 resulted in a significant performance decline. Therefore, the C/N range of 18:1–16:1 is likely the optimal condition for food waste treatment by BSFL and adjusting food waste C/N with urea could be a practical method for the performance improvement.AbstractBiowaste treatment by black soldier fly larvae (BSFL,Hermetia illucens) has received global research interest and growing industrial application. Larvae farming conditions, such as temperature, pH, and moisture, have been critically examined. However, the substrate carbon to nitrogen ratio (C/N), one of the key parameters that may affect larval survival and bioconversion efficiency, is significantly less studied. The current study aimed to compare the nitrogen supplying effects of 9 nitrogen species (i.e., NH4Cl, NaNO3, urea, uric acid, Gly, L-Glu, L-Glu:L-Asp (1:1,w/w), soybean flour, and fish meal) during food waste larval treatment, and further examine the C/N effects on the larval development and bioconversion process, using the C/N adjustment with urea from the initial 21:1 to 18:1, 16:1, 14:1, 12:1, and 10:1, respectively. The food wastes were supplied with the same amount of nitrogen element (1 g N/100 g dry wt) in the nitrogen source trial and different amount of urea in the C/N adjustment trial following larvae treatment. The results showed that NH4Cl and NaNO3caused significant harmful impacts on the larval survival and bioconversion process, while the 7 organic nitrogen species resulted in no significant negative effect. Further adjustment of C/N with urea showed that the C/N range between 18:1 and 14:1 was optimal for a high waste reduction performance (73.5–84.8%,p< 0.001) and a high larvae yield (25.3–26.6%,p= 0.015), while the C/N range of 18:1 to 16:1 was further optimal for an efficient larval protein yield (10.1–11.1%,p= 0.003) and lipid yield (7.6–8.1%,p= 0.002). The adjustment of C/N influenced the activity of antioxidant enzymes, such as superoxide dismutase (SOD,p= 0.015), whereas exerted no obvious impact on the larval amino acid composition. Altogether, organic nitrogen is more suitable than NH4Cl and NaNO3as the nitrogen amendment during larval food waste treatment, addition of small amounts of urea, targeting C/N of 18:1–14:1, would improve the waste reduction performance, and application of C/N at 18:1–16:1 would facilitate the larval protein and lipid bioconversion process.Keywords:nitrogen source;carbon to nitrogen ratio;food waste;urea;black soldier fly larvae;Hermetia illucens
研究领域:
环境科学
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昆虫学
·
废弃物处理
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Tao Zhou,Shuya Wu,Lianghu Su,Jianying Xiong,Youcai Zhao
Anaerobic fermentation liquid from food waste contains abundant nutrients, such as volatile fatty acids (VFAs) and NH3–N, which are promising nutrient sources for plant growth. Here, we report a novel integrated technique for the simultaneous recovery of C and N sources from anaerobic fermentation liquid. VFAs and NH3–N extracted from anaerobic fermentation liquid were synthesized and converted into layered double hydroxides (LDHs) and NH4HCO3, respectively, for subsequent use as soil amendments to provide external C and N to plants. The X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy/energy dispersive X-ray spectroscopy results suggested that VFAs were successfully intercalated within the LDH, and the composite (VFA-LDH) exhibited good sustained release performance. In the VFA-LDH synthesis process, the concentrations of chemical oxygen demand, VFAs, and NH3–N were reduced by 49.4%, 27.9%, and 43.6% in the anaerobic fermentation liquid, respectively. In addition, VFA-LDH and NH4HCO3 promoted increased soil fertility, including organic matter content, cation exchange capacity, and N and P contents. Overall, the developed technique exhibits great potential as an anaerobic fermentation liquid treatment and nutrient acquisition method.
研究领域:
环境科学
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农业科学
✓ 已验证
DOI
高影响因子
Biomass Conversion and Biorefinery
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2023
·
IF 4
Zhang Xian,Liu Ze,Khan Hafiz Ihsan Ul haq,Barati Bahram,Parakhonskiy Bogdan,Skirtach Andre G.,Rousseau Diederik P. L.,Van Hulle Stijn
Nitrogen source–modified biochar was produced from rice straw–derived biochar (RSBC) via pyrolysis carbonization using nitrogen sources such as ammonium chloride, dicyandiamide, melamine, thiourea, and urea. The characteristics of the resulting biochars were compared, including their elemental compositions, surface functional groups, and morphology. The N-modified biochar contained N- and O-based groups (N–H, C–N, C≡N, O–H, and C–O), primarily consisted of mesopores (3.01 ~ 14.42 nm pore diameter), and had a specific surface area ranging from 95.87 to 167.86 m2 g −1 . The N-modified biochar’s BPA adsorption capacity was correlated to the activation temperature (correlation coefficient n = 0.5), with adsorption capacities varying from 7.43 to 63.09 mg g −1 . Ammonium chloride–modified biochar synthesized at 800 °C had over twice the BPA adsorption capacity of pristine biochar (28.80 mg g −1 ) among various nitrogen sources evaluated. The pseudo-second-order model characterized the adsorption kinetics of BPA, while the Freundlich model faithfully captured its isotherms. This study emphasizes the significance of proper nitrogen source selection and optimal modification temperatures to enhance the BPA adsorption capacity of biochar. Graphical Abstract
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2016
·
IF 3.205
Dongrong Zeng,Xiang Yu,Yunfeng Zhan,Linmin Cao,Xiaoxian Wu,Bodong Zhang,Jilin Huang,Zhipeng Lin,Fangyan Xie,Weihong Zhang,Jian Chen,Weiguang Xie,Wenjie Mai,Hui Meng
Nitrogen doped carbon material works as non-noble-metal catalyst for the oxygen reduction reaction . In this work the doping effect on two different carbon material by two different nitrogen sources is studied. The carbon material studied is self-prepared carbon ribbon with higher graphitization degree compared with commercial Vulcan XC72 carbon powder. NH 3 and glycine are used as nitrogen sources which produce nitrogen containing functionalities after high temperature pyrolysis . NH 3 doping of carbon ribbon has no positive effect on the ORR activity, while an obvious increase in the activity is found for the glycine doped carbon ribbon. The NH 3 and glycine doped Vulcan carbon show similar activities which are better than undoped Vulcan carbon. The difference is explained by graphitization degree, nitrogen content and type of nitrogen containing functionalities.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）