ENVIRONMENTAL ENTOMOLOGY
·
2025
·
IF 1.5
Yang Jie,Wen Xi,Huang Xingrui,Zou Jie,Lu Yun,Yuan Fang,Xiao Sijie,Tang Xiaochao,Liu Zhixiao,Wu Zhengwei,Huang Xinglong
Pesticides released into the environment are increasingly recognized as a global threat to freshwater ecosystems because of their adverse effects on non-target organisms, particularly aquatic insects and other arthropods. Superoxide dismutases (SODs) are important antioxidant enzymes that play a crucial role in protecting organisms from oxidative stress induced by harmful materials. In this study, we identified 2 cellular SODs (PxSOD1 and PxSOD2) in Protohermes xanthodes Navás (Megaloptera: Corydalidae), an freshwater predatory insect, and determined the oxidative stress induced in P. xanthodes larvae by sublethal exposure to chlorpyrifos (CPF). PxSOD1 and PxSOD2 are members of the cytoplasmic Cu/ZnSODs and mitochondrial MnSODs, respectively, and differ substantially in protein structure. Both PxSOD1 and PxSOD2 recombinant proteins demonstrated catalytic activity toward O2•− in the activity assays. After exposure to sublethal concentrations of CPF, malondialdehyde (MDA) content and SOD activities were increased in P. xanthodes larvae in a dose-dependent manner. PxSOD1 expression was decreased in the 0.42 and 4.2 μg/L CPF groups and increased in the 4.2 μg/L CPF group. PxSOD2 was upregulated by 0.42, 4.2, and 8.4 μg/L CPF treatments and the expression levels in the 4.2 and 8.4 μg/L CPF groups were significantly higher than that in the no CPF control. Our results suggest that sublethal concentrations of CPF can induce oxidative stress in P. xanthodes larvae, and the cellular SODs in P. xanthodes larvae may contribute to the protection against CPF-induced oxidative stress.
研究领域:
昆虫学
·
环境毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Jiangong Jiang,Bingying He,Xueke Liu,Donghui Liu,Zhiqiang Zhou,Peng Wang
Pesticides and antibiotics are frequently present in aquatic environment which may pose potential risks to aquatic organisms. However, the interaction of pesticides and antibiotics in co-exposure model remains unclear. Here, the effects of the co-exposure of sulfamethazine (SMZ) on the toxicity and bioaccumulation of the organophosphorus insecticide chlorpyrifos (CPF) in zebrafish ( Danio rerio ) were explored. The 96-h LC 50 of chlorpyrifos to zebrafish was 1.36 mg/L and sulfamethazine at 1 mg/L slightly increased the acute toxicity with the 96-h LC 50 of 1.20 mg/L which was not significant. The 30-day co-exposure of chlorpyrifos with sulfamethazine at 1 mg/L aggravated the oxidative stress , decreased CarE and AChE activity, and increased CYP450 activity significantly. Furthermore, the co-exposure reduced the accumulation of chlorpyrifos and sulfamethazine while prolonged their depuration duration. The results demonstrated the exposure risk of chlorpyrifos to zebrafish may be enhanced in the presence of sulfamethazine.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2019
·
IF 3.298
Qiaojian Zhang,Shufang Zheng,Shengchen Wang,Wei Wang,Houjuan Xing,Shiwen Xu
Chlorpyrifos (CPF) has become a mainly pollution in water environment. Micro-RNAs (miRNAs) play an important part in the development of apoptosis and autophagy. However, the potential mechanism of CPF induced kidney toxicity and the roles of miRNAs are still unclear. To explore the underlying mechanism, the kidney of common carp exposed to different concentrations of CPF for 40 days was used as a research object. We found that CPF could damage the ultrastructure and function of kidney; and also caused antioxidant system disorder. CPF inhibited the mRNA level of miR-19a which improved AMP-activated protein kinase (AMPK). Furthermore, the detection of apoptosis and autophagy relative genes showed that the expressions of TSC complex subunit 2 (TSC2), light chain 3 (LC3), Dynein, tumor protein 53 (p53), Bcl-2 associated X protein (Bax), caspase-3 and caspase-9 were enhanced and the expressions of nechanistic target of rapamycin (mTOR), Ras homolog mTORC1 binding (Rheb) and B-cell lymphoma (Bcl-2) were reduced in dose-dependent way. Taken together, we conclude that CPF causes oxidative stress and miR-19a-AMPK axis disorder, thereby promotes apoptosis and autophagy in common carp kidney. Our study will provide theoretical basis for toxicology research and environmental protection of CPF.
研究领域:
毒理学
·
环境科学
·
分子生物学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Jun Hou,Fei Zhang,Peifang Wang,Chao Wang,Juan Chen,Yi Xu,Guoxiang You,Qing Zhou,Ziyu Li
To enhance the reduction of Chlorpyrifos (CP) in farmland drainage, zero valent iron (ZVI) was introduced into the anaerobic bioreactor through batch experiments. Compared to the mono-cell system, the coupled bio-ZVI system showed better performance on the removal of CP. The optimal operating conditions were determined to be 1 g L −1 of iron shavings, 800 μg L −1 of the initial CP concentration and 35 °C by comparing different ZVI types, ZVI dosages, initial CP concentrations and temperature. CP removal efficiency of 95.94% and 79.97% was obtained in the coupled bio-ZVI and control system under the optimal conditions, respectively. Scanning electron microscopy showed that iron shavings could enhance the granulation of the anaerobic sludge. Moreover, the relative abundances of species analyzed by high-throughput sequencing in the coupled system were higher than that in the control system. Especially, the highly enrichment of microorganisms capable of degrading CP or organophosphorus pesticide such as Cyanobacteria and Acinetobacter in the ZVI-amended system was detected. The main metabolite 3,5,6-trichloro-2-pyridinol (TCP) transformed from CP in the control system was higher than that in the coupled system, with the maximum levels of 253.84 μg L −1 and 221.71 μg L −1 , respectively. Besides, TCP in the coupled system can be degraded more significantly, which could suggest that the presence of ZVI could facilitate the resistance of the biomass to the toxicity of TCP.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xu Liu,Di Yang,Bingqian Liu
Given the irreversible neurotoxicity and high detection rates of chlorpyrifos (CPF) in agricultural products, developing sensitive and efficient sensors for CPF is highly urgent. Herein, we constructed a novel Locked-Activator-Cas12a (LAC) strategy with process “slimming” capability. It is predicated on a DNA activator that possesses two states acting as a crucial intermediary. The biosensor's efficacy is further enhanced by the copious generation of activators induced by the target, ensuring the unimpeded progression of the entire reaction, thereby facilitating precise and reliable CPF detection in complex matrices. Furthermore, we used bioinformatics resources, including AutodockVina, to explore the molecular interaction mechanism of CPF in binding to its aptamers through molecular docking simulations. Employing a two-step detection workflow, this approach is straightforward and readily scalable for detecting other organophosphorus pesticides by substituting the aptamer sequences. It is envisioned that these biosensors can be applied as a strategy robustness tool in agricultural samples, ultimately contributing to enhanced human health and well-being.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Long Wu,Wei Zeng,Bing Hu,Ting Wu,Min Zhou,Weihong Xie
Since pesticide residues in food have attracted increasing concern worldwide, it is crucial to develop rapid and sensitive analytical methods to detect pesticide residues and ensure food safety. In this work, via the biotin-streptavidin (SA) recognition system, we constructed a magnetic relaxation switching (MRS) immunosensor for sensitive detection of chlorpyrifos (CPF). With a competitive immunoassay mode, H 2 O 2 and Fe 2+ were firstly optimized as the reaction substrates. Wherein, horseradish peroxidase (HRP) acted as enzyme label to catalyze H 2 O 2 and mediated the conversion of Fe 2+ /Fe 3+ . By introducing Fe 2+ /Fe 3+ interconversion as MRS signal output, CPF was detected by the immunoreaction induced variations of the transverse relaxation time (ΔT 2 ). The proposed MRS immunosensor exhibited a detection linear range from 0.01 to 1000 ng mL −1 (R 2 = 0.9916), with the limit of detection (LOD) of 6 pg mL −1 (3S/M, n = 3). As a proof-of-concept, CPF was easily detected among five different pesticides at a low concentration level (0.1 ng mL −1 ), as well as in real samples (apple, tea, and lettuce) with recoveries of 80.70–115.30%. Besides, the sensor can realize one step of “separation and detection” towards CPF with the aid of magnetic nanoparticles , which demonstrate its promising potential for pesticide residue detection in food samples and environment.
研究领域:
食品安全
·
分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Shangyun Lu,Shuo Liu,Jinling Cui,Xiaoyi Liu,Chong Zhao,Lihong Fan,Shutao Yin,Hongbo Hu
Patulin (PAT) is the most common food-borne mycotoxin found in fruits and fruit-derived products, while chlorpyrifos (CPF) is a widely used pesticide on fruit and other crops. On the basis of the residue data, certain types of fruits can be contaminated simultaneously by patulin and chlorpyrifos. However, there are no available data about the combined toxicity. Since liver is a possible toxic target of both patulin and chlorpyrifos, we tested whether the combination exposure can cause enhanced hepatotoxicity using both cell culture and animal models. Results showed that the combination resulted in synergistic cytotoxicity in vitro and significantly enhanced liver toxicity in vivo. Mechanistically, PAT inhibited catalase activity via PIG3 induction, while CPF decreased catalase expression. These two mechanisms were converged in response to the combination, leading to enhanced inactivating catalase and boosted reactive oxygen species generation. The finding implicated that it is necessary to consider the combined toxicity in safety assessment of these food-borne contaminants.
研究领域:
毒理学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Urological Science
·
2019
·Vol. 30
·期 5
·206
·
IF 0
Upadhyay Jyoti,Tiwari Nidhi,Rana Mahendra,Bisht Satpal S.
Background: Pesticide exposure has the potential for causing acute or chronic health effects at certain developmental stages of life. It can result in irreversible damage to structure and function of an organ. The exposure of females to pesticides before or after pregnancy is a matter of concern because of the possibility of having a child born with congenital disorders and developmental defects. Prenatal exposure of any toxicants can program the developing foetus to develop diseases that manifest in later life. Very few reports are available related to the pesticide exposure induced morphological changes in kidney which has prompted us to undertake this research study. Aim: The aim of this study is to evaluate the effect of pesticide, chlorpyrifos on the kidney development of rat neonates after being exposed prenatally to pesticides throughout their gestational period. Materials and Methods: Virgin female wistar rats weighing 200-250 gms were mated with adult males. It consisted of twelve, divided into two sub groups six rats per each were treated with selected pesticides, chlorpyrifos (insecticide) readily available commercially for various agricultural and non-agricultural settings. Insecticide chlorpyrifos was given at a dose of 10mg/kg per orally to the female rats throughout their gestation period. After gestation period rat neonates were sacrificed and their kidney section was processed for histopathological analysis. The SEM study of chlorpyrifos exposed kidney section of rat neonates was performed, investigating changes in morphometric and ultra-structural features of kidney in rat neonates. Results: The histopathological analysis shows morphological changes in the kidneys of prenatally pesticides exposed rat neonates markedly different from control group. Chlorpyrifos exposed neonates kidney shows damaged kidney corpuscles and glomeruli. Conclusion: This study concluded that pesticide exposure during gestation period to pregnant rats causes significant renal toxicity in their neonates.
研究领域:
毒理学
·
发育生物学
·
肾脏病学
·
环境健康
·
生殖毒理学
·
分子病理学
✓ 已验证
DOI
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Huanqi Zhang,Xiaozhe Chen,Fuqing Liu,Yanju Bi,Meichen Gao,Xiaojing Li
Long-term environmental exposure to chlorpyrifos (CPF) can cause intestinal damage. Baicalein (BAI) helps aquatic organisms resist environmental pollution stress. Adenosine 5′-monophosphate activated protein kinase (AMPK) is a key molecule in energy regulation; however, the mechanism by which BAI inhibits CPF-induced autophagy/ferroptosis remains unclear. Based on reported environmental concentrations and IC 50 values of CPF, we established models using the midgut of carp and Epithelioma papulosum cyprini (EPC) cells exposed to CPF with or without BAI treatment. Transcriptomics and network pharmacology were used to analyze the key pathways affected by CPF and BAI. Tissue damage, cell cycle regulation, autophagy, ferroptosis, and mitochondrial function were assessed. The effect of BAI on AMPK–unc-51-like autophagy-activating kinase 1 (ULK1) binding was confirmed by molecular dynamics simulation, co-immunoprecipitation, and laser confocal analysis. In addition, the regulatory impact of an AMPK activator on the autophagy/ferroptosis axis was examined. Results indicated that BAI alleviated CPF-induced pathological changes and mitochondrial damage. CPF exposure caused tight junction disruption, mitochondrial depolarization, and reactive oxygen species and mitochondrial reactive oxygen species production. Transcriptomic analysis revealed that CPF toxicity is related to phagosomes, cell cycle regulation, and ferroptosis. By targeting AMPK, BAI prevents the formation of the AMPK–ULK1 complex and inhibits the CPF-induced cascade involving excessive autophagy, cell cycle arrest, and ferroptosis. In conclusion, BAI targets AMPK to prevent binding with ULK1, thereby suppressing the CPF-activated autophagy/ferroptosis axis, reversing mitochondrial dysfunction, and restoring intestinal homeostasis.
研究领域:
环境毒理学
·
分子生物学
·
药理学
·
细胞生物学
·
生态毒理学
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产品关联
DOI
PubMed
高影响因子
Photonics Research
·
2025
·Vol. 13
·期 5
·1158-1164
·
IF 7.2
Tianqing Zhou,Binggang Xiao,Yong Du,Jianyuan Qin
Pesticide residues in tea are an important problem affecting the sustainable development of the tea industry; thus, pesticide detection is the key to ensuring the quality and safety of tea. Here, a terahertz metasurface structure based on the quasi-bound state in the continuum is proposed, which consists of two copper microrods arranged periodically. This design in the metasurface provides strong local enhancement near the surface of the microstructure, significantly improving the interaction of light with the analyte, resulting in increased sensitivity. The simulated and experimental results show that the metasurface structure can be used to detect the refractive index of trace analytes with a high sensitivity and successfully detect low concentrations of chlorpyrifos in tea. This study provides a new idea for the detection of pesticide residues in tea.
研究领域:
光学
·
传感器
·
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiaolu Sun,Chaomin Gao,Lina Zhang,Mei Yan,Jinghua Yu,Shenguang Ge
A photoelectrochemical (PEC) sensor based on hierarchical branched titanium dioxide nanorods (B-TiO 2 NRs) modified with molecularly imprinted polymer (MIP) was constructed for sensitive and efficient detection of chlorpyris (CPF). B-TiO 2 NRs were grown directly on fluorine doped tin oxide substrate by the hydrothermal method and employed as matrix for immobilization of MIP. P-aminothiophenol (ATP) and CPF were assembled on the surface of B-TiO 2 NRs by formation of hydrogen-bonding interactions through electropolymerization in the MIP preparation process. The MIP was characterized by scanning electron microscopy, and further confirmed by cyclic voltammetry to prove the successful synthesis. In the proposed sensing platform, the photocurrent response is inversely proportional to the CPF concentration because the insulating layer hinders the harvesting of light and electron transfer with increasing of CPF concentration. Moreover, the MIP based B-TiO 2 NRs (MIP-B-TiO 2 NRs) had an excellent recognition capacity for specific detection CPF over other pesticides. The PEC sensing platform is developed for the detection of CPF in the linear range from 0.01 ng mL −1 to 100 ng mL −1 with a low detection limit of 7.4 pg mL −1 . The proposed sensor offered a promising platform for application in detecting pesticide residuals and other environment deleterious chemicals with excellent sensitivity and selectivity, low interference and high stability.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2024
·
IF 3.9
Mengcheng Zhuo,Xi Wang,Yanhong Shi,Kun Chen,Xuchun Qiu
Organophosphorus pesticides (OPs), such as chlorpyrifos (CPF), are the most commonly used pesticides worldwide. Considering that OPs will eventually enter aquatic ecosystems due to runoff from agricultural lands, accidental leakage, and other unforeseen emergencies, monitoring water pollution of those substances is crucial for environmental protection and public health. In this study, Japanese medaka ( Oryzias latipes ) were exposed to CPF (0.03, 0.06, and 0.12 mg/L) for 6 h, and the time-series variations in their locomotor behavior and vocal traits were investigated. Compared with that measured before exposure, significantly changed locomotor behavior and vocal traits in Japanese medaka exposed to CPF could be observed at 4 h after exposure and thereafter, and the pattern of behavioral changes depends on the CPF concentrations. Exposure to CPF also changed the frequency-sound pressure level curve of Japanese medaka at 6 h after exposure, especially at 0.12 mg/L. Moreover, CPF exposure could significantly inhibit the acetylcholinesterase (AChE) activity in the brains and eyes of medaka, which exhibited significant correlations with the variation of locomotor behavioral and vocal traits. Considering that inhibiting the AChE activity is the primary mechanism underlying the neurobehavioral toxicity of all OPs, our finding suggested that simultaneously monitoring changes in the locomotor behavioral and vocal traits has a high potential to reflect the pollution of organophosphorus substances.
研究领域:
环境科学
·
毒理学
·
水生生物学
·
生态毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）