JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Haiyun Li,Yachun Ma,Tuo Yao,Li Ma,Jiangui Zhang,Changning Li
In the process of microbial degradation of pyrethroid pesticides, the synergistic effect of the microbial community is more conducive to the complete degradation of toxic compounds than a single strain. At present, the degradation pathway of pyrethroids in a single strain has been well revealed, but the synergistic metabolism at the community level has not been well explained. This study elucidated the bacterial community succession, metabolic pathway, and phytotoxicity assessment during β-cyfluthrin biodegradation by a novel bacterial consortium enriched from contaminated soil. The results showed that the half-life of β-cyfluthrin at different initial concentrations of 0.25, 0.5, 0.75, and 1.0 mg mL–1 were 4.16, 7.34, 12.81, and 22.73 days, respectively. Enterobacter was involved in β-cyfluthrin degradation metabolism in the initial stage, and other bacterial genera (Microbacterium, Ochrobactrum, Pseudomonas, Hyphomicrobiaceae, Achromobacter, etc.) significantly contribute to the degradation of intermediate metabolites in the later stages. Functional gene prediction and metabolite analysis showed that xenobiotic biodegradation and metabolism, especially benzoate degradation and metabolism by cytochrome P450 were the major means of β-cyfluthrin degradation. Further, two degradation pathways of β-cyfluthrin were proposed, which were mainly ester hydrolysis and oxidation to degrade β-cyfluthrin through the production of carboxylesterase and oxidoreductase. In addition, the inoculated bacterial consortium could degrade β-cyfluthrin residues in water and soil and reduce its phytotoxicity in Medicago sativa. Hence, this novel bacterial consortium has important application in the remediation environments polluted by β-cyfluthrin.
研究领域:
环境科学
·
微生物学
·
生态毒理学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Chao Hu,Cong Zhang,Yi-Fan Tang,Yu-Xi Liu,Zi-Nan Xia,Yan Wang,Wei-Tu Li,Ping Gao,Yu-Ting Li,Yun-Tong Lv,Xue-Qing Yang
Insecticides are commonly utilized in agriculture and forestry for pest control, but their dispersal can pose hazards to humans and environment. Understanding resistance, inheritance patterns, and fitness costs can help manage resistance. A λ-cyhalothrin-resistant population (LCR) of Cydia pomonella, a global pest of pome fruits and walnuts, was obtained through selective insecticide breeding for 15 generations, showing stable moderate resistance (23.85-fold). This population was cross-resistant to deltamethrin (4.26-fold) but not to β-cypermethrin, chlorantraniliprole, chlorpyrifos, and avermectin. Genetic analysis revealed the resistance was autosomal, incompletely dominant, and controlled by multiple genes. Increased activity of glutathione S-transferases and cytochrome P450 monooxygenases (P450s) played a primary role in resistance, with specific genes up-regulated in LCR, and exhibited significant expression in midgut. LCR also exhibited fitness costs, including delays in development, reduced fecundity, and slower population growth. These findings contribute to understanding λ-cyhalothrin resistance in C. pomonella and can guide resistance management strategies.
研究领域:
农业昆虫学
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分子生物学
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害虫管理
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Kai Chen,Gaobo Yu,Furui He,Qingfeng Zhou,Dunchao Xiao,Jiacheng Li,Yuhong Feng
Alginate (Alg) was grafted on the surface of anisotropic silica (SiO 2 -x) via the Ugi reaction (Alg-SiO 2 -1, Alg-SiO 2 -2, and Alg-SiO 2 -4). Compared with pristine SiO 2 -x, modified SiO 2 -x is more sensitive to pH. Three stable liquid paraffin-in-water emulsions were prepared with Alg-SiO2-1, Alg-SiO2-2, and Alg-SiO2-4. Alg-SiO 2 -2 exhibited satisfactory emulsification ability. The emulsions became more stable as emulsion pH varied from 2.0 to 6.2 because of polymer chain interactions that led to the formation of a three-dimensional network. When the emulsion pH varied from 6.2 to 8.0, the particle charge increased, in turn increasing interparticle the electrostatic interactions that increased emulsion stability. When the emulsion pH was 9.0, the subsequent decrease in particle charge, decreased the emulsion stability. The model drug λ-cyhalothrin was embedded in the emulsions. A sustained-release assay demonstrated that increasing emulsion pH from 3.0 to 8.0 decreased cumulative drug release from the emulsion from 99.7% to 13.5%. This result indicated that the emulsion is a pH triggered drug delivery system. The sustained-release curves of λ-cyhalothrin are describable by the Weibull model.
研究领域:
材料科学
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药物递送
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环境科学
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DOI
PubMed
高影响因子
LANGMUIR
·
2013
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IF 4.187
Jianming Pan,Linzi Li,Hui Hang,Runrun Wu,Xiaohui Dai,Weidong Shi,Yongsheng Yan
Magnetic/hollow double-shelled imprinted polymers (MH-MIPs) were synthesized by Pickering emulsion polymerization. In this method, attapulgite (ATP) particles were used as stabilizers to establish a stable oil-in-water emulsion, and a few hydrophilic Fe3O4 nanoparticles were allowed to be magnetic separation carriers. The imprinting system was fabricated by radical polymerization in the presence of the functional and polymeric monomers in the oil phase. The results of characterization indicated that MH-MIPs exhibited magnetic sensitivity (Ms = 4.76 emu g–1), thermal stability (especially below 200 °C), and hollow structure and were composed of exterior ATP shells and interior imprinted polymers shells. Then MH-MIPs were evaluated as sorbents for the selective binding of λ-cyhalothrin as a result of their magnetism, enhanced mechanical strength, hydrophilic surface, and recognition ability. The kinetic properties of MH-MIPs were well described by the pseudo-second-order equation, indicating that the chemical process could be the rate-limiting step in the adsorption process for λ-cyhalothrin. The equilibrium adsorption capacity of MH-MIPs was 60.06 μmol g–1 at 25 °C, and the Langmuir isotherm model gave a better fit to the experimental data, indicating the monolayer molecular adsorption for λ-cyhalothrin. The selective recognition experiments also demonstrated the high affinity and selectivity of MH-MIIPs toward λ-cyhalothrin over fenvalerate and diethyl phthalate.
研究领域:
材料科学
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环境科学
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分析化学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 4
·1452-1466
·
IF 4.462
Pei-Rong Li,Yu Shi,Di Ju,Yu-Xi Liu,Wei Wang,Ying-Shi He,Yu-Yun Zhang,Xue-Qing Yang
BACKGROUND The evolution of insect resistance to pesticides poses a continuing threat to sustainable pest management. While much is known about the molecular mechanisms that confer resistance in model insects and few agricultural pests, far less is known about fruit pests. Field-evolved resistance to synthetic insecticides such as lambda -cyhalothrin has been widely documented in Cydia pomonella , a major invasive pest of pome fruit worldwide, and the increased production of cytochrome P450 monooxygenases (P450s) has been linked to resistance in field-evolved resistant populations. However, the underlying molecular mechanisms of P450-mediated insecticide resistance remain largely unknown . RESULTS Here we found that functional redundancy and preference of metabolism by P450s genes in the CYP9A subfamily confer resistance to lambda -cyhalothrin in Cydia pomonella . A total of four CYP9A genes, including CYP9A61 , CYP9A120 , CYP9A121 , and CYP9A122 , were identified from Cydia pomonella . Among these, CYP9A120 , CYP9A121 , and CYP9A122 were predominantly expressed in the midgut of larvae. The expression levels of these P450 genes were significantly induced by a lethal dose that would kill 10% (LD 10 ) of lambda -cyhalothrin and were overexpressed in a field-evolved lambda -cyhalothrin resistant population. Knockdown of CYP9A120 and CYP9A121 by RNA-mediated interference (RNAi) increased the susceptibility of larvae to lambda -cyhalothrin. In vitro assays demonstrated that recombinant P450s expressed in Sf9 cells can metabolize lambda -cyhalothrin, but with functional redundancy and divergence through regioselectivity of metabolism. CYP9A121 preferred to convert lambda -cyhalothrin to 2′-hydroxy- lambda -cyhalothrin, whereas CYP9A122 only generated 4′-hydroxy metabolite of lambda -cyhalothrin. Although possesses a relatively low metabolic capability, CYP9A120 balanced catalytic competence to generate both 2′- and 4′-metabolites. CONCLUSION Collectively, these results reveal that metabolic functional redundancy of three members of the CYP9A subfamily leads to P450-mediated lambda -cyhalothrin resistance in Cydia pomonella , thus representing a potential adaptive evolutionary strategy during its worldwide expansion. © 2022 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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杀虫剂抗性
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DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Rong Zhao,Wei Jia,Youfa Jiang,Xiaoju Wu,Xuefeng Jiang,Saijie Song,Jun Zhang,He Shen,Jian Shen
Lambda-Cyhalothrin (LC) is a high-efficiency and broad-spectrum insecticide. However, due to its poor water dispersion stability and unstable efficacy, it has many problems in practical applications. Therefore, there is an urgent need to develop new formulations with stability, low toxicity, and environmental friendliness to overcome the drawbacks of traditional pesticide formulations. This study designed an iron-based metal–organic framework (MIL-101(Fe)) as a nanocarrier for the loading of LC and then surface modified it with polydopamine (PDA) to form the nanopesticide PDA-LC-MIL. The results showed that PDA-LC-MIL has good stability and can achieve controlled release of pesticide by adjusting the pH value. In addition, it also has a good leaf affinity and can effectively adhere on crop leaves, improving the utilization rate of pesticides. The insecticidal experimental results indicate that PDA-LC-MIL has significantly improved sustained insecticidal activity. PDA-LC-MIL also has good biocompatibility and shows no significant inhibitory effect on seed germination and seedling growth of Vigna radiata (L.) Wilczek and maize. Therefore, we firmly believe that MIL-based nanopesticides will have broad application prospects in the field of green pesticides and modern agriculture.
研究领域:
农业科学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Jixiang Wang,Lin Gao,Donglai Han,Jianming Pan,Hao Qiu,Hongji Li,Xiao Wei,Jiangdong Dai,Jinghai Yang,Hui Yao,Yongsheng Yan
In this study, fluorescent molecularly imprinted polymers (FMIPs), which were for the selective recognition and fluorescence detection of λ-cyhalothrin (LC), were synthesized via fluorescein 5(6)-isothiocyanate (FITC) and 3-aminopropyltriethoxysilane (APTS)/SiO2 particles. The SiO2@FITC-APTS@MIPs were characterized by Fourier transform infrared (FT-IR), UV–vis spectrophotometer (UV–vis), fluorescence spectrophotometer, thermogravimetric analysis (TGA), confocal laser scanning microscope (CLSM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The as-synthesized SiO2@FITC-APTS@MIPs with an imprinted polymer film (thickness was about 100 nm) was demonstrated to be spherically shaped and had good monodispersity, high fluorescence intensity, and good selective recognition. Using fluorescence quenching as the detection tool, the largest fluorescence quenching efficiency (F0/F – 1) of SiO2@FITC-APTS@MIPs is close to 2.5 when the concentration of the LC is 1.0 μM L–1. In addition, a linear relationship (F0/F – 1= 0.0162C + 0.0272) could be obtained covering a wide concentration range of 0–60 nM L–1 with a correlation coefficient of 0.9968 described by the Stern–Volmer equation. Moreover, the limit of detection (LOD) of the SiO2@FITC-APTS@MIPs was 9.17 nM L–1. The experiment results of practical detection revealed that the SiO2@FITC-APTS@MIPs as an attractive recognition element was satisfactory for the determination of LC in Chinese spirits. Therefore, this study demonstrated the potential of SiO2@FITC-APTS@MIPs for the recognition and detection of LC in food.
研究领域:
分析化学
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食品安全检测
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材料科学
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DOI
PubMed
高影响因子
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·Vol. 109
·期 4
·e21859
·
IF 1.698
Yan Liu,Shuangyu Li,Hongjia Yang,Yaru Chen,Dong Fan
Carboxylesterases are one of the three major types of detoxification enzyme in insects. In this study, we screened 12 full-length carboxylesterase cDNA sequences from the oriental armyworm Mythimna separata ; they were named MsCarE1–MsCarE12 and registered in GenBank with accession numbers MK440541 – MK440552. Treatment of fourth instar larvae of M. separata with the LD 50 of the insecticide chlorantraniliprole increased the expression levels of MsCarE3 and MsCarE4 , while treatment with the LD 50 of lambda-cyhalothrin significantly increased the expression levels of MsCarE5 and MsCarE10 . Spatiotemporal expression detection showed that MsCarE3 , MsCarE4 , MsCarE5 , and MsCarE10 were expressed at different developmental stages and in different tissues of M. separata and their expression levels were different. Induction using a high dose of chlorantraniliprole resulted in lower expression of MsCarE3 and MsCarE4 . LD 50 of lambda-cyhalothrin induced higher expression of MsCarE5 and MsCarE10 , while LD 70 induced higher MsCarE10 expression at 3, 6, and 12 h after treatment. RNA interference successfully inhibited the expression of MsCarE3 , MsCarE4 , MsCarE5 , and MsCarE10 , to different degrees at different time points. Silencing of MsCarE5 , or MsCarE5 and MsCarE10 simultaneously changed carboxylesterase activity and increased the susceptibility of M. separata larvae to lambda-cyhalothrin. This study provides a new method to increase the insect susceptibility to insecticide. Research Highlights Twelve carboxylesterase cDNA sequences named MsCarE1–MsCarE12 were identified from Mythimna separata. Silence of MsCarE5, or MsCarE5and MsCarE10 simultaneously increased the insecticidal activity of lambda-cyhalothrin on M. separatalarvae.
研究领域:
昆虫学
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分子生物学
·
害虫管理
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DOI
PubMed
高影响因子
Micro & Nano Letters
·
2019
·Vol. 14
·期 1
·81-85
·
IF 0.975
Yakun Liu,Baohua Guo
Microcarriers containing pesticide have been designed to improve drug stability and increase release efficiency, which is favourable to the environment and agriculture development. In this work, biodegradable poly(butylene succinate) microspheres containing pesticide, λ-cyhalothrin, as an active agent are prepared by the solvent evaporation induced phase separation method. The microspheres show a uniform morphology and a narrow particle size distribution. The pesticide content is measured by high-performance liquid chromatography. The prepared microcarriers demonstrate high-loading capacity, encapsulation efficiency and long release time, which may find potential applications as new pesticide formulations.
研究领域:
材料科学
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农业科学
·
环境科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Yuli Wang,Saijie Song,Xiaohong Chu,Wenli Feng,Jie Li,Xinrong Huang,Ninglin Zhou,Jian Shen
In recent years, stimuli-responsive controlled-release pesticide formulations have drawn extensive as they show huge potential in the practical application of agriculture. Herein, a multifunctional composite nanocarrier was prepared by our group, Poly(N-isopropylacrylamide) modified graphene oxide (PNIPAm-GO), this is a temperature-responsive release pesticide formulation that can regulate pesticide release based on the temperature change. Lambda-cyhalothrin (LC) was loaded on PNIPAm-GO nanocomposite carrier via a simple physisorption process and LC@PNIPAm-GO exhibited temperature-responsive controlled release performance leading to release of LC at a specified temperature and extended its persisted release time. In addition, the LC@PNIPAm-GO controlled-release pesticide formulations showed both good water solubility and dispersion stability while its anti-UV property. Furthermore, we also assessed the biological safety of LC@PNIPAm-GO and proved that PNIPAm-GO is non-toxic. The study of this temperature-responsive pesticide formulation has promoted the development of intelligent pesticide formulations to great extent.
研究领域:
农业科学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 4
·期 9
·4435-4443
·
IF 2.562
Jianming Pan,Wenjing Zhu,Xiaohui Dai,Xuesheng Yan,Mengyin Gan,Linzi Li,Hui Hang,Yongsheng Yan
Magnetic molecularly imprinted microcapsules (MMIMs) were synthesized by Pickering emulsion polymerization. In this work, an oil-in-water Pickering emulsion stabilized by halloysite nanotubes (HNTs) was first established in the presence of a few hydrophilic Fe3O4 nanoparticles as magnetic separation carriers. The imprinting system was fabricated by radical polymerization and subsequent combination with functional and polymeric monomers in the oil phase. The formation mechanism of the Pickering emulsion and MMIMs is discussed in detail, and the as-prepared MMIMs are successfully evaluated as sorbents for the recognition of λ-cyhalothrin. The results demonstrated that the MMIMs exhibited magnetic sensitivity (Ms = 8.45 emu g−1), a hollow structure, a semipermeable external surface and excellent robustness. The batch mode experiments proved that the imprinting effect synchronously improved the adsorption kinetics and equilibrium for the MMIMs. The selective recognition experiments also suggested high affinity and selectivity of MMIMs towards λ-cyhalothrin over fenvalerate and diethyl phthalate.
研究领域:
材料科学
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环境科学
·
化学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 4.321
Yijie Yin,Jianming Pan,Jun Cao,Yue Ma,Guoqing Pan,Runrun Wu,Xiaohui Dai,Minjia Meng,Yongsheng Yan
Herein we reported a novel method for preparation of hybrid molecularly imprinted polymer foams (MIPFs) via a twice imprinting strategy. Firstly, based on halloysite nanotubes (HNTs) cores, three molecularly imprinted nanomaterials (MIPs-S, MIPs-M and MIPs-L) with controlled thickness of imprinted shells (i.e., 7.0 nm, 17 nm and 36 nm, respectively), were prepared by precipitation polymerization. The surface hydrophobicity of MIP nanomaterials gradually enhanced with the increasing thickness of imprinted shells. Secondly, three hybrid MIP foams (MIPFs-S, MIPFs-M, MIPFs-L) were successively fabricated by W/O Pickering high internal phase emulsions (HIPEs) template stabilized by the corresponding MIPs nanomaterials. Due to the increased hydrophobicity of the MIPs nanomaterials, the amount of interconnecting pores in hybrid MIPFs was continuously decreased until a closed-cell structure appeared, while the average void diameter increased from 10 μm to 28 μm. Binding experiments demonstrated that optimized hybrid MIP foams (MIPFs-M) possessed enhanced adsorption capacity, fast kinetics and excellent recognition ability towards λ-cyhalothrin (LC). Therefore, this two-step imprinting strategy could not only figure out the difficulty in solid–liquid phase separation and the recycling of nano-sized MIPs but also overcome the disadvantage of stabilizing solid particles on the surface of MIPFs referring to the lack of specific adsorption activity.
研究领域:
材料科学
·
环境科学
·
化学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）