MOLECULES
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2022
·Vol. 27
·期 9
·3027
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IF 4.927
Tingting Li,Haiqiong Ma,Hong Li,Hao Tang,Jinwen Huang,Shiying Wei,Qingxia Yuan,Xiaohuo Shi,Chenghai Gao,Shunli Mi,Longyan Zhao,Shengping Zhong,Yonghong Liu
Laminaria japonicais widely consumed as a key food and medicine. Polysaccharides are one of the most plentiful constituents of this marine plant. In this study, several polysaccharide fractions with different charge numbers were obtained. Their physicochemical properties and anticoagulant activities were determined by chemical and instrumental methods. The chemical analysis showed thatLaminaria japonicapolysaccharides (LJPs) and the purified fractions LJP0, LJP04, LJP06, and LJP08 mainly consisted of mannose, glucuronic acid, galactose, and fucose in different mole ratios. LJP04 and LJP06 also contained minor amounts of xylose. The polysaccharide fractions eluted by higher concentration of NaCl solutions showed higher contents of uronic acid and sulfate group. Biological activity assays showed that LJPs LJP06 and LJP08 could obviously prolong the activated partial thromboplastin time (APTT), indicating that they had strong anticoagulant activity. Furthermore, we found that LJP06 exerted this activity by inhibiting intrinsic factor Xase with higher selectivity than other fractions, which may have negligible bleeding risk. The sulfate group may play an important role in the anticoagulant activity. In addition, the carboxyl group and surface morphology of these fractions may affect their anticoagulant activities. The results provide information for applications ofL. japonicapolysaccharides, especially LJP06 as anticoagulants in functional foods and therapeutic agents.Keywords:Laminaria japonica;polysaccharides;physicochemical properties;anticoagulant activity
研究领域:
海洋生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
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2020
·Vol. 18
·期 9
·444
·
IF 4.073
Bin Wei,Qi-Wu Zhong,Song-Ze Ke,Tao-Shun Zhou,Qiao-Li Xu,Si-Jia Wang,Jian-Wei Chen,Hua-Wei Zhang,Wei-Hua Jin,Hong Wang
A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention. In this study, five polysaccharides were prepared fromSargassumfusiformeand their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated. The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and fourSargassumfusiformepolysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia. Sf-1 and Sf-A could also significantly prevent HFD-induced weight gain. Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance ofOscillospira,Mucispirillum, andClostridiales. Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance ofMucispinllumandOscillospira. Receiver operating characteristic analysis indicated thatMucispinllumandOscillospiraexhibited good discriminatory power (AUC = 0.745–0.833) in the prediction of fasting hypoglycemia. Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles ofMucispinllumandOscillospirain the biological activity of SFPs.Keywords:Sargassum fusiformepolysaccharides;high-fat diet;gut microbiota;early fasting hypoglycemia
研究领域:
营养学
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微生物学
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代谢病研究
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
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IF 5.279
Songze Ke,Yanlei Yu,Qiaoli Xu,Bo Zhang,Sijia Wang,Weihua Jin,Bin Wei,Hong Wang
Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application. In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice. The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group. LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function. Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera. Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
研究领域:
营养学
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微生物学
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DOI
PubMed
高影响因子
Frontiers in Nutrition
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2024
·
IF 4
Jiang Long,Song Chen,Ai Chunqing,Wen Chengrong,Song Shuang
The present study demonstrated the digestion behavior and fermentation characteristics of a sulfated polysaccharide from Sargassum fusiforme (SFSP) in the simulated digestion tract environment. The results showed that the molecular weight of two components in SFSP could not be changed by simulated digestion, and no free monosaccharide was produced. This indicates that most of SFSP can reach the colon as prototypes. During the fermentation with human intestinal flora in vitro, the higher-molecular-weight component of SFSP was utilized, the total sugar content decreased by 16%, the reducing sugar content increased, and the galactose content in monosaccharide composition decreased relatively. This indicates that SFSP can be selectively utilized by human intestinal flora. At the same time, SFSP also changed the structure of intestinal flora. Compared with the blank group, SFSP significantly increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes. At the genus level, the abundances of Bacteroides and Megamonas increased, while the abundances of Shigella, Klebsiella, and Collinsella decreased. Moreover, the concentrations of total short-chain fatty acids (SCFAs), acetic, propionic and n-butyric acids significantly increased compared to the blank group. SFSP could down-regulate the contents of trimethylamine, piperidone and secondary bile acid in fermentation broth. The contents of nicotinic acid, pantothenic acid and other organic acids were increased. Therefore, SFSP shows significant potential to regulate gut microbiota and promote human health.
研究领域:
食品科学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
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2025
·
IF 5.7
Xue-Wu Chen,Jun Long,Quan Zhang,Linghong Huang,Xin-Yuan Sun
s: Background: Calcium oxalate (CaOx) crystal deposition and its resultant cellular oxidative damage and inflammation are important causes of renal stone formation. It is clinically important to conduct research on multifunctional anti-stone drugs targeting these predisposing factors. Methods: We modified natural Undaria pinnatifida polysaccharide (UPP0) by sulfation via the sulfur trioxide-pyridine method, resulting in four sulfated polysaccharides with varying sulfate group (–OSO3–) contents: 1.59% (UPP0), 6.03% (UPP1), 20.83% (UPP2), and 36.39% (UPP3), and compared their differences in the inhibition of crystalline formation, renal injury, and inflammation in the process of renal stone formation at chemical and cellular levels. Results: The UPPS were able to inhibit the nucleation, growth and aggregation of CaOx crystals in vitro. Among them, UPP3 with the maximum sulfate group content showed the greatest crystallization inhibition ability. The nucleation inhibition and aggregation inhibition of UPP3 at a concentration of 0.5 mg/mL were as high as 80.21% and 72.34%, respectively. The CaOx crystal size regulated by UPP3 was significantly reduced from 25.9 ± 2.8 μm to 5.9 ± 1.2 μm. Furthermore, UPPS were observed to up-regulate the expression of the antioxidant enzyme superoxide dismutase (SOD) in cells, reduce the levels of ROS and malonaldehyde (MDA), enhance lysosomal integrity, decrease intracellular Ca2+ levels, inhibit the decline in mitochondrial membrane potential, reduce the production of cellular inflammatory factors (TNF-α, MCP-1, IL-18, and IL-1β), and ultimately inhibit cell apoptosis. Conclusion: UPPS combine multiple biological functions of crystallization regulation, antioxidant and anti-inflammatory, and have important potential in the prevention of kidney stones. Sulfation modification can improve the biological activity of UPP0 and provide a reference for screening and optimization methods of stone drugs.
研究领域:
医学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Yujiao Sun,Shuting Hou,Shuang Song,Bao Zhang,Chunqing Ai,Xuefeng Chen,Ning Liu
This study comparatively evaluated the effects of different extraction methods on yield, structural features and antioxidant activities of Laminaria japonica ( L. japonica ) polysaccharides . Results showed that acid, water and alkaline extracted L . japonica polysaccharides (LJP-CA, LJP-W and LJP-A, respectively) differed significantly. Among three polysaccharides, LJP-W showed the highest viscosity with high uronic acid content and average molecular weight, whereas LJP-CA showed the lowest viscosity with low uronic acid and high sulfate content with moderate average molecular weight. LJP-CA was mainly composed of fucose , mannose and galactose , but the proportion of glucose was greatly increased in LJP-W and LJP-A. LJP-W had an even and smooth sheet-like appearance, while LJP-CA and LJP-A exhibited irregular and rough fragments or particles with stronger antioxidant activities. These results suggest that acid and alkali would propose an improved process for polysaccharide preparation from L . japonica and to exploit its potential application as a functional ingredient in food application.
研究领域:
食品科学
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海洋生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Kexin Huang,Kuiyou Wang,Shida Wu,Xueqian Li,Xinghua Gao,Yun Xiong,Fengyu Bai,Xunyu Song,Mingqian Tan,Wentao Su
Fucoxanthin (FX) has very interesting biological activities, but its low water solubility, stability, and bioavailability limit its applications. This study developed the one-step manufacturing strategy for FX delivery system based on 2, 2, 6, 6-Tetramethylpiperidine-1-oxyl (TEMPO)-oxidized kelp nanocellulose (TKNC), which significantly improved the environmental stability of the hydrophobic substance FX, as well as its intestinal retention ability and bioavailability. TKNC was successfully prepared by TEMPO oxidation and showed good water dispersibility, high specific surface area, and high FX loading capacity. Compared with free FX, this delivery system significantly improved the stability of FX under ultraviolet irradiation, high temperature, and gastrointestinal digestion conditions. In vitro cell experiments demonstrated that this system can significantly reduce the level of reactive oxygen species induced by lipopolysaccharides, and significantly inhibit the release of pro-inflammatory factors, showing its excellent anti-inflammatory ability. In vivo biodistribution experiments further presented that TKNC prolonged the residence time of the Nile Red in the intestine and enhanced its ability to penetrate the intestinal barrier, ultimately significantly increasing the enrichment in the liver of mice. These findings provide a new strategy for improving the stability and bioavailability of hydrophobic active substances.
研究领域:
食品科学
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材料科学
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生物学
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产品关联
DOI
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Chun-Feng Xue,Teng Wu,Wei Zhao,Yue-Yue Wei,Kai Lv,Xiao-Qing Li,Wen-Jun Yan,Xiao-Hong Li,Xiao-Gang Hao
In order to achieve attractive energy density and specific capacitance of electrochemical supercapacitor , multi-heteroatom self-doped carbon is prepared by pyrolyzing the aquatic biomass undaria pinnatifida (UP) filled with magnesium acetate (Mg(CH 3 COO) 2 ). The pyrolysis product of magnesium oxide (MgO), acting as rigid filling, can shape the pore and help to keep the original pore system . The resultant immersed-undaria-pinnatifida-based activated carbon (IUPAC-700) co-doped with multi-heteroatom shows specific surface area of 1116 m 2 g −1 , pore volume of 1.63 cm 3 g −1 and desired pore size distribution. The electrode basing on it delivers specific capacitance of 579.7 F g −1 at the current density of 0.50 A g −1 and 306.0 F g −1 at 100.0 A g −1 in the electrolyte of 1.0 M H 2 SO 4 . It exhibits a high specific capacitance of 488.6 F g −1 in the two-electrode system and provides a significant energy density of 28.7 W kg −1 at the power density of 164.0 W kg −1 . It also displays perfect cyclic life after 8000 times charge-discharge at the current density of 5.0 A g −1 . The assembled supercapacitor can power the LED lights. The sample IUPAC-700 featured with self-doped multi-heteroatoms is a very competitive electrode material for energy storage device with desired comprehensive performance.
研究领域:
材料科学
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能源存储
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongshuai Jing,Cheng Tao,Hao Zhang,Ziying Wang,Hanju Fan,Beibei Hu,Lanfang Wu
Facing the clinical challenge of uncontrolled bleeding and infection after trauma, it is urgent to obtain a new type of dressing with antibacterial and wound healing promoting activities and good biological safety. Polysaccharide was extracted from Laminaria japonica Aresch by water extraction and alcohol precipitation, and LA-1 was obtained after separation and purification by DEAE-52 cellulose column. LA-1 had →2,6)-β-Manp-(1→) as the main chain and →4)-OAC-β-Galp-(1→, →3)-α-Fucp(1→, →4)-β-ManpA-(1→ and →4). Then, A kind of (PSx%AN) membrane with hemostatic and antibacterial functions was prepared by solution casting with LA-1 as green reducing agent and stabilizer. The (PSx%AN) film was formed by the cross-linking reaction of LA-1, AgNO 3 , polyvinyl alcohol and starch (3:1), in which the starch interacted with the polyhydroxy structure of polyvinyl alcohol to form hydrogen bonds, which endowed the film with good mechanical strength and biocompatibility. In order to comprehensively evaluate the performance of (PSx%AN) membrane, the oxidation resistance, antibacterial activity and cytotoxicity were tested, and animal experiments were also carried out. The results of the anti-oxidation and anti-bacterial experiments showed that the (PSx%AN) film not only exhibited good anti-oxidation performance, but also demonstrated a strong inhibitory effect on S. aureus and E. coli , with the maximum inhibition zone diameters of 16.8 ± 0.3 mm and 11.0 ± 0.2 mm respectively. The full-thickness skin defect experiment demonstrated that the (PS1%AN) membrane exhibited superior results compared to other groups on the 11th day. Specifically, it effectively increased the hydroxyproline concentration to 4.69 ± 0.19 μg/mL and the total protein content to 551.09 ± 7.19 μg/mL. Additionally, it reduced inflammatory responses, shortened wound healing time in mice, and promoted tissue repair. These findings provide a novel perspective for the (PSx%AN) membrane as a potential hemostatic and anti-infective wound dressing.
研究领域:
生物材料学
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医学工程
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Jinlan Nie,Xiaoting Fu,Lei Wang,Jiachao Xu,Xin Gao
This study evaluated the efficacy of Monascus purpureus fermentation on Saccharina japonica (SJ). Healthy substances and antioxidant activity of fermented SJ (FSJ) were determined. Results showed that fermentation caused the release of phenolic compounds and flavonoids, which resulted in the enhancement of antioxidant activity. Essential amino acids and γ-aminobutyric acid also greatly accumulated in FSJ. Sensory evaluation and gas chromatography-ion mobility spectrometry (GC-IMS) were used to evaluate flavor properties of FSJ. A lexicon consisted of 24 descriptors was established for SJ and FSJ, of which 14 descriptors were regarded as odor attributes. A total of 46 volatile compounds were identified by GC-IMS and showed positive correlation with odor attributes. Fifteen volatile compounds were screened as key compounds, tricarboxylic acid cycle, embden-meyerhof-parnas and amino acid catabolism were main formation metabolisms of them. Advanced properties of FSJ indicated that fermentation is a promising approach for the production of SJ food.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Oxidative Medicine and Cellular Longevity
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2021
·
IF 6.543
Wei-Bo Huang,Guo-Jun Zou,Gu-Hua Tang,Xin-Yuan Sun,Jian-Ming Ouyang
The original Laminaria polysaccharide (LP0) was sulfated using the sulfur trioxide-pyridine method, and four sulfated Laminaria polysaccharides (SLPs) were obtained, namely, SLP1, SLP2, SLP3, and SLP4. The sulfated (–OSO3–) contents were 8.58%, 15.1%, 22.8%, and 31.3%, respectively. The structures of the polysaccharides were characterized using a Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance (NMR) techniques. SLPs showed better antioxidant activity than LP0, increased the concentration of soluble Ca2+ in the solution, reduced the amount of CaOx precipitation and degree of CaOx crystal aggregation, induced COD crystal formation, and protected HK-2 cells from damage caused by nanometer calcium oxalate crystals. These effects can inhibit the formation of CaOx kidney stones. The biological activity of the polysaccharides increased with the content of –OSO3−, that is, the biological activities of the polysaccharides had the following order: LP0 < SLP1 < SLP2 < SLP3 < SLP4. These results reveal that SLPs with high –OSO3− contents are potential drugs for effectively inhibiting the formation of CaOx stones.
研究领域:
药理学
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材料科学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2020
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IF 5.774
Xian-chao Shang,Depeng Chu,Ji-xu Zhang,Yan-fen Zheng,Yiqiang Li
Based on natural deep eutectic solvents (DESs), microwave-assisted green extraction was applied for production of polysaccharides from bladder-wrack ( Fucus vesiculosus ). In this study, nine different combinations of deep eutectic solvents were evaluated for extraction of polysaccharides from bladder-wrack, and the results showed that DES system composed of choline chloride and 1,4-butanediol with molar ratio of 1:5 possessed the optimal extraction efficiency for polysaccharides. A three-level and four-variable Box-Behnken design (BBD), a specific design of response surface methodology (RSM), was used to determine the optimum extraction conditions for the maximum yields of polysaccharides from bladder-wrack by using DESs. The maximum yields of polysaccharides attained 116.33 mg/g within DES water content of 32%, extraction time of 35 min, extraction temperature of 168 °C and solid–liquid ratio of 39 mg/mL. After partial separation and purification of the polysaccharides extracted by DESs, antioxidant activity and anticancer activity in vitro were evaluated. The results revealed that polysaccharides extracted from bladder-wrack exhibited excellent antioxidant activities in vitro including DPPH and ABTS radical-scavenging activity. For human cervical cancer HeLa cells, the polysaccharides possessed a strong inhibition effect on the growth rate. So these results indicate that deep eutectic solvent, as an environmentally-friendly solvent, can be applied to extract polysaccharides from multifarious plant materials.
研究领域:
海洋生物学
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天然产物化学
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药理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）