JOURNAL OF ANALYTICAL CHEMISTRY
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2020
·Vol. 75
·期 11
·1430-1434
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IF 0.84
Yuling Gao,Liang Xilong,Sun Peng,Guo Yongxia
Abstract A novel method for the determination of butachlor and fipronil in liquid milk has been developed using ionic liquid dispersive liquid-liquid microextraction coupled with ultra-high performance liquid chromatography ( UHPLC ). The optimum extraction conditions were determined to be 60 μL of 1-octyl-3-methyl imidazole six fluorine phosphate extraction solvent, 1000 μL of acetonitrile as the dispersing solvent, vortex extraction time of 3 min, centrifugation for 5 min at room temperature at 10 000 rpm. After extraction, 30 μL of extraction solvent was obtained from the sample for UHPLC analysis. It was shown that good linearity was obtained with butachlor and fipronil concentrations from 5 to 1000 μg/kg with correlation coefficient of 0.999. The average recoveries were in the range of 90 to 104% at spiked levels from 10 to 50 μg/kg with intra-day relative standard deviations lower than 3.7% ( n = 6) and inter-day relative standard deviations lower than 6.1% ( n = 3). The limits of detection were 0.5 and 0.3 μg/kg for butachlor and fipronil, respectively.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2024
·
IF 2.5
Hongchao Dai,Lei Yuan,Luyao Fan,Jia Yang,Xinan Jiao
This work assessed the prevalence and risk-related phenotypes of Bacillus cereus in milk products. Bacillus was the most dominant genus of 113 collected strains from 41 milk samples, and B. cereus (19.27%) was the most abundant species showing a high genetic diversity with seven random amplified polymorphic DNA patterns. Most B. cereus isolates were strong biofilm formers with good motility, extracellular polymeric substance-producing ability, high proteolytic and lipolytic activities. Antibiotic resistance analysis suggested the majority B. cereus strains were resistant to oxacillin (90.48%), tetracycline (95.24%), and penicillin G sodium (47.62%). Results revealed the genotypic diversity of B. cereus , and their potential risk in dairy industry.
研究领域:
微生物学
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食品安全
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乳品科学
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 81
·期 3
·539-544
·
IF 1.401
Gao Yuling,Xu Chong,Liu Shaobin,Sun Peng
Abstract A simple, rapid, and environmentally friendly method has been developed for the determination of seven pyrethroid pesticides in liquid milk by dispersive liquid–liquid microextraction based on the solidification of a floating organic droplet coupled with GC. Several variables that affect the extraction efficiency, including the type and volume of the extraction solvent and those of the dispersing solvent, the extraction time, and the mass fraction of salt were optimized. Under the optimum conditions, good linearity was observed between 1.0 and 500.0 μg L −1 , with regression analysis yielding an r value (correlation coefficient) of > 0.9990 for the determination of the seven pyrethroid pesticides in liquid milk. The recoveries of the target analytes from liquid milk at spiking levels of 5.0, 10.0, and 20.0 μg L −1 were between 90.2 and 108.4%. LODs for the analytes were 0.08–0.44 μg L −1 , while LOQs were 0.24–1.47 μg L −1 . The intraday ( n = 6) and interday ( n = 3) RSDs were less than 5.2 and 7.5%, respectively. The proposed method was successfully applied to determine the seven pyrethroid pesticides in real liquid milk. Graphical Abstract
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Abid Hussain,Hongbin Pu,Bingxue Hu,Da-Wen Sun
Surface-enhanced Raman spectroscopy based on thioglycolic acid (TGA) functionalized silver-coated gold nanoparticles (Au@Ag-TGANPs) was developed for the facile screening of thiabendazole (TBZ) and ferbam (0.025–10 ppm) in liquid milk for the first time. Results showed that silver-coated gold nanoparticles (Au@AgNPs) with a core size of 32 nm and a shell thickness of 5 nm was successfully modified with 3 nm TGA. The sensitive Au@Ag-TGANPs could enhance TBZ and ferbam signals by factors of 6.4 × 10 4 and 9.8 × 10 4 , respectively, and achieved the detection of TBZ and ferbam with limits of detection of 0.12 and 0.003 ppm, R 2 of 0.988 and 0.9821, percent recoveries of 88–103% and of 87.2–103.5%, and relative standard deviations of 4.1–9.2% and 3.5–8.3%, respectively. The current simple and green method could thus be used to detect other unsafe chemicals in future studies.
研究领域:
食品安全
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分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Abid Hussain,Hongbin Pu,Da-Wen Sun
A cysteamine functionalized core shelled nanoparticles (Au@Ag-CysNPs) was presented for simultaneous and rapid detection of sodium thiocyanate (STC) and benzoic acid (BA) preservatives in liquid milk using surface-enhanced Raman spectroscopy (SERS) technique. A spectrum covering 350–2350 cm −1 region was selected to detect STC with concentrations ranging from 0.5 to 10 mg/L and BA with concentrations ranging from 15 to 240 mg/L in milk samples. Characterization of nanoparticles using high-resolution TEM confirmed that the successful synthesis of Au@AgNPs with core (gold) size of 28 nm and shell (silver) thickness of about 5 nm was grafted with 120 μL of 0.1 nM cysteamine hydrochloride. Results showed that Au@Ag-CysNPs could be used to detect STC up to 0.03 mg/L with a limit of quantification (LOQ) of 0.039 mg/L and a coefficient of determination (R 2 ) of 0.9833 in the milk sample. For detecting BA, it could be screened up to 9.8 mg/L with LOQ of 10.2 mg/L and R 2 of 0.9903. The proposed substrate was also highly sensitive and the employed method involved only minor sample pretreatment steps. It is thus hoped that the new substrate could be used in the screening of prohibited chemicals in complex food matrices in future studies.
研究领域:
分析化学
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食品科学
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纳米技术
·
光谱学
✓ 已验证
DOI
高影响因子
Materials Today Advances
·
2023
·
IF 10
Tonghua Wan,Wei Song,Hongli Wen,Xue Qiu,Qiuqiang Zhan,Wei Chen,Huijuan Yu,Lin Yu,Abdur Raheem Aleem
Tobramycin (TOB) is a broad-spectrum aminoglycoside extensively used as veterinary medicine to treat bacterial infections. However, TOB residue in food and environment may cause great threat to human health. Here, we report, for the first time, the upconversion nanoparticles (UCNPs) for TOB detection based on Förster resonance energy transfer (FRET). The upconversion (UC) luminescence from core-shell structured UCNPs was effectively quenched after addition of MnO 2 nanosheets due to the efficient energy transfer from the UCNPs to MnO 2 nanosheets. When interacting with TOB, the UC luminescence was restored and increased as a result of coupling between TOB and aptamer, with MnO 2 nanosheets dropping off the latter one. The UCNPs@SiO 2 -MnO 2 aptasensors for TOB detection have a good selectivity and sensitivity in a linear range of 10–180 ng mL −1 with a limit of detection (LOD) of 3.04 ng mL −1 . The sensors were tested for TOB detection in real samples of liquid milk and fishes and the results are very good and satisfactory. These type of aptasensors are promising for practical applications as they have a high sensitivity, selectivity, low LOD, and are autofluorescence-free.
研究领域:
分析化学
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纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Tongtong Jia,Shaolun Ouyang,Wenrui Chen,Ting Zhang,Mengqi Lu,Xiuying Zhou,Hongtao Lei,Xiaoqun Wei
To get rid of the singularity of the detection of veterinary drug residues in liquid milk, a high-throughput determination was developed for 126 VDRs in 17 categories in liquid milk based on the one-step extraction coupled with LC-HRMS (UPLC-Q-Exactive-Orbitrap-MS). The results showed that adding 2.5 mL of 0.1 mol/L Na2EDTA-McIlvaine in acetonitrile-methanol extractant (50:50, V/V) could effectively improve the response intensity of veterinary drug residues in liquid milk, reduce the matrix effect and eliminate the need for expensive SPE purification. The established external standard quantitative method has a LOD of 0.01–0.30 μg/L for all categories of veterinary drug residues, good linearity in the concentration range of LOQ of 0.03–1.00 μg/L, and correlation coefficients (r 2 ) were all greater than 0.995. The spike recoveries at all three levels were between 80.30% and 119.95%. The positive detection rate of this method in 128 actual samples was 8.59%.
研究领域:
分析化学
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食品科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Jiangxue An,Li Li,Yaping Ding,Weijuan Hu,Dingding Duan,Huan Lu,Daixin Ye,Xiaoli Zhu,Hongxia Chen
A novel electrochemical sensor based on molecularly imprinted polymer (MIP) and iron metal-organic frameworks was fabricated for highly sensitive detection of melamine (MEL) in this work. MIL-53(Fe), a kind of iron metal-organic frameworks, was prepared by a simple solvothermal method . The molecularly imprinted polymer films with specific recognition sites were synthesized by electropolymerization using methacrylic acid (MAA) as functional monomers interacted with MEL as templates. MIL-53(Fe) was characterized by XRD and FT-IR. The morphology of the MIP modified electrode was characterized by scanning electron microscopy. Under the optimal conditions, the molecularly imprinted electrochemical sensor (MIECS) shows a wide linear range of 10 −11 M to 10 −6 M for MEL with a detection limit (3σ) as low as 8.21 × 10 −12 M. Moreover, the MIP sensor shows high selectivity for melamine, good reproducibility and stability. The MIECS has been applied to the real detection of melamine in liquid milk samples with satisfying results.
研究领域:
分析化学
·
材料科学
·
电化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）