Animals
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2022
·Vol. 12
·期 18
·2480
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IF 3.231
Cheng Zhang,Weihao Li,Ligong Chen,Zhaoliang Chen,Xuejing Wang,Qianqian Xu,Hailong Zhang,Huan Chen,Juxiang Liu
Simple SummaryDeveloping safe and effective antibiotic growth promoters (AGPs) substitutes is particularly important to improve animal health and production performance. As an essential plant oil, the oregano oil's main bioactive substance is carvacrol, which has been proven to have antioxidant, anti-inflammatory, antibacterial, and antiviral properties. The sanguinarine from macleaya cordata is the primary bioactive substance. Sanguinarine has anti-tumor, immune-enhancing, antibacterial, and anti-inflammatory effects. However, it has not been reported whether the compatibility of oregano oil and macleaya cordata extract could produce better results. This study is the first to report the effect of oregano oil combined with macleaya cordata oral solution on the growth of broilers. The oregano oil combined with macleaya cordata oral solution significantly improved the growth performance of broilers. At the same time, serum biochemical indices, serum antioxidant indices, serum immune indices, serum cytokines, and intestinal morphology were significantly improved. In summary, our results demonstrated that the mixed solution of oregano oil and macleaya cordata has substantial potential to be an alternative to AGPs for broilers to reduce costs and improve benefits. This study provides basic data and technical support for further research.AbstractThe abuse of AGPs in animal husbandry has led to severe problems such as drug resistance and ecological, and environmental destruction, which seriously threaten human health and public health security. In recent years, extracts of oregano oil and macleaya cordata have become a hot spot in the research and application of AGP substitutes for their safety and high efficiency. This study is the first to report the effect of oregano oil combined with macleaya cordata oral solution on broiler growth performance. A total of 960 one-day-old broiler chickens were randomly divided into four treatment groups (240 chickens per group). Each treatment group was divided into six replicate groups (40 birds per replicate group). There were four groups in this study: the solvent control group, the oregano essential oil combined with macleaya cordata extract oral solution group (OS group), the oregano essential oil oral solution group (OEO group), and the macleaya cordata extract oral solution group (MCE group). Two chickens from each replicate group were collected and mixed into a composite sample. Six composite samples were obtained for each treatment group. The results showed that the oregano oil combined with macleaya cordata oral solution significantly improved the growth performance of broiler chickens. At the same time, serum biochemical indices, serum antioxidant indices, serum immune indices, serum cytokines, and intestinal morphology were significantly improved by the OS group. This study shows that oregano oil combined with macleaya cordata oral solution has substantial potential to be an alternative to AGPs for broilers.Keywords:oregano oil;macleaya cordata;oral solution;broilers;growth performance
研究领域:
动物科学
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药理学
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兽医学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Mingen Fei,Xueliang Chen,Han Chen,Yongtong Fan,Yongchen Peng,Yu-Chung Chang,Wendi Liu,Renhui Qiu
Polymer mortar, consisting of a polymer binder and aggregates, offers superior properties such as enhanced strength, durability, and corrosion resistance compared to the traditional cement-based mortars. Within the polymer-based mortar, epoxy resin is one of the most commonly used resins owing to its high performance. However, its widespread adoption is hindered by costs and environmental concerns, particularly the dependence of bisphenol A-containing epoxide, a substance harmful to human health. In this work, a novel epoxy hardener derived from oregano oil (OEO) was developed to create a more eco-friendly and cost-effective binder for polymer mortar. The inclusion of OEO-based hardeners reduced the viscosity of the binder and accelerated the curing process. The Mannich base structure of the hardener induced more reactive sites in the epoxy system, resulting in a denser crosslinked network. Polymer mortars fabricated with this novel hardener exhibited improved mechanical properties, bonding strength, and corrosion resistance compared to conventional epoxy mortars. Scanning electron microscopy images also revealed a stronger interfacial bond between the epoxy resin and the aggregates. This study demonstrates the potential of OEO-based hardeners to enhance the performance of epoxy binders, providing a sustainable alternative for polymer-based mortar applications.
研究领域:
建筑材料学
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环境科学
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有机化学
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DOI
高影响因子
JOURNAL OF CULTURAL HERITAGE
·
2024
·
IF 3.5
Yulan Hu,Lichao Chen,Liwei Pan,Bingjian Zhang,Qiang Li
The study created an electrospun fiber membrane with polyacrylonitrile, nano silver, and oregano oil using the electrospinning technique. After examining the preparation parameters, an appropriate formula for the composite film spinning solution and electrospinning parameters were found: 11 wt percent polyacrylonitrile, 6 wt percent natural oregano essential oil, 15 kV of spinning voltage, 0.6 mL/h of advancing rate, and 20 cm of spinning distance. The composite nanofiber membrane demonstrated improved shape and structure, a more balanced antibacterial effect, and persistence against microbes when subjected to the prescribed formula and circumstances. In the end, the strong antimicrobial qualities of the nano-fiber material were validated by the Feilaifeng field testing. This study showed that when combined with natural plant essential oil, the composite nanofiber membrane has potential for use as a green microbial control material for Grottoes historic heritage. By preventing an intense direct contact between antimirobial agents and the cultural relics of the grottoes, the composite barrier reduced the potential harm that could be caused by the solution soaking into the rocks. Moreover, the composite nanofiber membrane provides the advantages of large-scale antibacterial activity from natural essential oil and long-lasting antimicrobial capabilities from nanosilver. It also has no effect on the cultural relics of the Grotto, is simple to remove, and leaves little trace.
研究领域:
材料科学
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微生物学
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文化遗产保护
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2022
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IF 10.383
Huimin Chen,Jing Cheng,Xixi Cai,Jinzhi Han,Xu Chen,Lijun You,Caihua Xiong,Shaoyun Wang
Biofilm infection will cause chronic inflammation and hinder the normal healing process of wound. Here, based on the self-assembly of three designed amphiphilic pentapeptides named EK, GG, and DR, pH-switchable antibacterial hydrogels with amphiphilic fiber network are used for the eradication of biofilms and the rescue of delayed healing in infected wounds. These pentapeptides-based hydrogels exhibit an acidic pH-switchable antimicrobial effect and are biocompatible at neutral pH. Additionally, supramolecular nanofiber networks with physical cross-linking with thermosensitive polymers (PNIPAm) and loaded antibacterial oregano oil are further developed. In vitro experiments indicate that the antimicrobial activity of hydrogels comes from the disassembly of acidic pH-dependent nanofiber network and activated release of pentapeptides and oregano oil, which achieves synergistic biofilm eradication. Remarkably, DR-based supramolecular hydrogel improves the healing efficiency of the full-thickness wound of skin in vivo, which is manifested by increased wound closure rate, reduced inflammatory response, faster angiogenesis, and collagen deposition in the wound, exhibiting great potential as wound dressing. The proposed synergistic strategy of inhibiting biofilm formation and activating healing may provide an efficient method for the treatment of clinically infected wounds.
研究领域:
材料科学
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生物材料
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药学
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伤口愈合
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感染控制
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产品关联
DOI
PubMed
高影响因子
Veterinary Sciences
·
2025
·Vol. 12
·期 12
·1206
·
IF 2.3
Yi Lu,Zhenyue Li,Zitong Yang,Ran Zhu,Mengxi Yan,Zhuhua Liu,Mingli Liu,Yuan Wang,Jue Wang,Qi Wang,Juxiang Liu,Cheng Zhang,Xuejing Wang,Huan Cui
Simple SummaryAntibiotic growth promoters have been banned or strictly restricted in many countries, creating an urgent need for safe and effective alternatives to support broiler health and performance. Oregano essential oil andMacleaya cordataextract are two phytogenic additives with promising antimicrobial, antioxidant, and anti-inflammatory properties, but their combined effects in broilers are not fully understood. In this study, 960 broilers were randomly assigned to four treatments: a control group, a group receiving oregano essential oil (OEO), a group receivingMacleaya cordataextract (MCE), and a group receiving a combination of the two (OMS). The combined OMS treatment improved growth performance and feed efficiency, enhanced serum antioxidant and immune indices, and modulated fecal microbiota by enriching beneficial bacteria and reducing potentially harmful taxa. These findings suggest that the combination of OEO and MCE can be used as a practical phytogenic strategy to improve broiler productivity and gut health in antibiotic-free production systems.With the growing demand for antibiotic-free and sustainable poultry production, plant-derived antimicrobials have emerged as promising alternatives. However, a systematic understanding of the combined effects of oregano essential oil (OEO) andMacleaya cordataextract (MCE) on the broiler gut microbiome remains lacking. This study employed an integrated “structure–function–phenotype” framework to investigate the individual and combined (OMS) effects of OEO and MCE on gut microecological remodeling and its coupling with host growth, metabolic, and immune phenotypes. A total of 960 one-day-old broiler chicks were individually weighed and then randomly allocated to four treatments using body-weight-stratified randomization, with 6 replicate pens per treatment and 40 birds per pen, to ensure similar initial body weight across groups. Over a 42-day trial, we evaluated growth performance, serum biochemistry, antioxidant status, and immune parameters. Compared to the control, the OMS treatment significantly enhanced average daily feed intake (ADFI) and average daily gain (ADG), increased serum total protein (TP), and decreased blood urea nitrogen (BUN), triglycerides (TG), total cholesterol (TC), and alkaline phosphatase (ALP). However, the feed-to-gain ratio (F/G) was also higher in the OMS group, indicating that the improvement in growth rate did not translate into enhanced feed efficiency but was primarily driven by increased feed consumption. OMS also improved overall antioxidant capacity and key enzyme activities, elevated immunoglobulin levels, and reduced pro-inflammatory cytokines. Notably, OMS maintainedLactobacillusdominance, enrichedBacteroides, Enterococcus, andButyricicoccus, and reducedEscherichia–Shigella. Functional predictions via PICRUSt2 suggested enhanced metabolic pathways related to antioxidant and immune functions; however, these results represent inference-based predictions and should be interpreted cautiously. Overall, the combination of OEO and MCE exerted synergistic benefits on growth, physiological health, and gut microbiota, supporting its potential as a phytogenic strategy for antibiotic-free broiler production.
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Si Shi,Junjie Tang,Yanyan Zhou,Hongyan Huang,Jianxi Zhang,Wenhao Liu,Zhiwen Hu,Hongwei Wang,Jie Pang,Chunhua Wu
Maintaining the safety and quality of minimally processed agricultural products is a significant challenge in modern food supply chains. This study developed multifunctional sodium alginate (SA)-based films incorporating high internal phase Pickering emulsions (HIPPEs) stabilized by curcumin/β-cyclodextrin inclusion complexes (CUR/β-CD). The HIPPEs demonstrated excellent colloidal stability (>21 days) with a mean droplet diameter of 12.76 ± 0.32 μm. Through solvent casting, HIPPEs were uniformly integrated into SA matrices (0.5–2 % w/w). Based on a comprehensive characterization of the films, the optimum concentration of 1.5 % for doping with HIPPEs was determined. The SA/HIPPEs1.5 films exhibited excellent tensile strength (14.21 MPa), elongation at break (26.28 %), and adequate water vapor (6.5 × 10 −11 g/(m·s·Pa)) and oxygen permeability (1.06 × 10 −3 g/m 2 ·s). Furthermore, they demonstrated dual antimicrobial-antioxidant functionality, with inhibition zones of 19.30 ± 0.70 mm ( Staphylococcus aureus ) and 20.53 ± 0.40 mm ( Escherichia coli ), along with 70.63 % DPPH radical scavenging activity. Compared to polyethylene packaging, SA/HIPPEs1.5 films effectively preserved blueberry quality at 25 °C for 18 days, showing lower weight loss (30.6 % vs. 42.2 %) and a favorable pH (3.43 vs. 4.22). These findings support the potential of SA/HIPPEs composite films as an effective active packaging solution for extending blueberry shelf life.
研究领域:
食品科学
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包装技术
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胶体与界面科学
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高分子科学
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农产品保鲜
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
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2024
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IF 3.7
Lingyu Yin,Yuhang Cao,Meihui Wang,Baohua Kong,Qian Liu,Hui Wang,Hao Wang
The zein-quercetin covalent complexes were prepared by alkali treatment (A-ZEIN-Q) and free radical grafting (R-ZEIN-Q) and subsequently followed by encapsulation of oregano essential oil (OEO), and alkali grafted zein-quercetin covalent nanoparticles (A-ZQNPs-OEO) and free radical grafted zein-quercetin covalent nanoparticles (R-ZQNPs-OEO) were synthesized respectively. Additionally, the particle size, ζ-potential, microstructure, encapsulation efficiency, release behaviour, surface hydrophobicity, stability, antioxidant activity, and antibacterial activity of these nanoparticles were characterized. The results of infrared spectroscopy showed that a covalent bond was formed between zein and quercetin, the particle size of the nanoparticles decreased, and the degree of densification was higher than that of zein nanoparticles. Unlike the aggregated zein nanoparticles, the zein-quercetin nanoparticles were dispersed more uniformly. In addition, the encapsulation efficiency of OEO in A-ZQNPs-OEO and R-ZQNPs-OEO increased significantly from 45.11 % to 73.42 % and 70.90 % to that of OEO in single zein nanoparticles, respectively. Among the four nanoparticles, A-ZQNPs-OEO shows the highest stability, while the OEO-encapsulated quercetin covalent nanoparticles show better-sustained release performance, lower surface hydrophobicity, and excellent antioxidant and antibacterial activities. Overall, the synthesized OEO encapsulated zein-quercetin covalent nanoparticles possess the ability to be applied in food preservation and other applications.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Qiaoqiao Liu,Yangcheng Xu,Hang Su,Zheliang Nie,Xin Shi,Bingcheng Ge,Shuyan Gao
The widespread use of conventional non-degradable packaging films for fruit preservation has led to serious environmental concerns, highlighting the urgent need for sustainable, biodegradable, and multifunctional packaging materials. Herein, we developed a carboxymethyl cellulose/pectin/oregano essential oil/vanillic acid (CMC/pectin/OEO/VA) film, in which hydrogen bonding between CMC and pectin hydroxyl groups enhances structural integrity and mechanical properties, further strengthened by VA, while phenolic compounds such as carvacrol and thymol in OEO provide antioxidant and antimicrobial functions. The optimized CMC/Pectin/OEO/VA0.06 (CPOV0.06) films demonstrate outstanding comprehensive performance, including excellent mechanical properties (tensile strength: 49.24 MPa), remarkable antioxidant capacity (DPPH radical scavenging rate: 85.87 %), significant antimicrobial effects ( E. coli : 6.33 mm, S. aureus : 6.00 mm), and complete biodegradability (soil assimilation time: 10 days). In addition, the blend film effectively prolonged the shelf life of cherry tomatoes up to 19 days under ambient storage conditions. This film offers a sustainable packaging approach, effectively addressing both food preservation and plastic pollution challenges.
研究领域:
食品科学
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材料科学
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环境科学
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包装技术
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农业科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuhang Cao,Lingyu Yin,Fei Li,Yi Deng,Baohua Kong,Qian Liu,Hao Wang,Hui Wang
Chilled pork retains most of its nutrients but is prone to deterioration during the production-to-consumption process. To address this issue this study aimed to develop zein-Arabic gum composite nanoparticles loaded with oregano essential oil (ZAG-OEO) and incorporate them into sodium alginate films to enhance the freshness and shelf life of chilled pork. Sodium alginate, known for its excellent film-forming properties, was selected as the matrix to prepare ZAG-OEO-containing sodium alginate films (SA-ZAG-OEO). The results revealed that the tensile strength and elongation at break of the prepared films were 47.73 ± 2.15 MPa and 6.27 ± 0.21 %, respectively, at a 2.5 % nanoparticle concentration. The water contact angle of the films incorporating nanoparticles reached 81.5 ± 1.95°. The incorporation of nanoparticles enhanced the thermal stability and antibacterial activity of the films. The prepared films were utilized for the storage of chilled pork, and the quality changes were analyzed. The results demonstrate that SA-ZAG-OEO films inhibit microbial growth and lipid oxidation, thereby delaying pork spoilage. This study offers new insights into extending the shelf life of chilled pork and developing advanced meat preservation methods for the future development of the meat industry.
研究领域:
食品科学
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材料科学
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包装技术
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肉类工业
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yu Duan,Dexiang Wang,Shengnan Li,Minkang Hu,Boyang Qin,Yu Lv,Haijun Du,Jun Feng
Carvacrol has widely used in the field of medicine and sterilization. Thus, developing reliable, efficient, and rapid method for the carvacrol detection is meaningful. In this study, we fabricated a carvacrol sensor based on Pb 0.95 Ti 0.05 O decorated with nitrogen self-doped biochar (Pb 0.95 Ti 0.05 O/NC), which was synthesized by hydrothermal and carbonization of soybean root nodules, respectively. The prepared sensor exhibited better electrochemical performances than that of PbO, Pb 0.95 Ti 0.05 O, NC. The fabricated Pb 0.95 Ti 0.05 O/NC sensor exhibited a wide detection range from 0.1 to 190 µ M and a low detection limit of 0.054 µ M. The excellent electrochemical performances were attributed to the increased electron density and electron mobility after the introduction of Ti doping and nitrogen doped carbon. Mechanistic studies revealed that the electrochemical oxidation of carvacrol involves one electron and was limited by adsorption process. The sensor demonstrated good stability, anti-interference, reproducibility, and good repeatability. Moreover, the sensor displayed excellent recovery rates ranging from 92.7% to 102.9% when applied to the detection of carvacrol in real samples, making it a promising candidate for practical applications in the sensitive detection of carvacrol.
研究领域:
分析化学
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食品科学
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医学
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产品关联
DOI
高影响因子
MICROBIAL PATHOGENESIS
·
2024
·
IF 3.3
Peng Guo,Zhenye Li,Ting Cai,Du Guo,Baowei Yang,Chunling Zhang,Zhongguo Shan,Xin Wang,Xiaoli Peng,Guorong Liu,Chao Shi,Metab Alharbi,Abdullah F Alasmari
Listeria monocytogenes ( L. monocytogenes ) is a prevalent foodborne pathogen with a remarkable capacity to form biofilms on utensil surfaces. The Listeriolysin O (LLO) exhibits hemolytic activity, which is responsible for causing human infections. In this study, we investigated the inhibitory effect and mechanism of oregano essential oil (OEO) on L. monocytogenes , evaluated the effects on its biofilm removal and hemolytic activity. The minimum inhibitory concentration (MIC) of OEO against L. monocytogenes was 0.03 % ( v / v ). L. monocytogenes was treated with OEO at 3/2 MIC for 30 min the bacteria was decreased below the detection limit (10 CFU/mL) in PBS and TSB (the initial bacterial load was about 6.5 log CFU/mL). The level of L . monocytogenes in minced pork co-cultured with OEO (15 MIC) about 2.5 log CFU/g lower than that in the untreated group. The inhibitory mechanisms of OEO against planktonic L. monocytogenes encompassed perturbation of cellular morphology, elevation in reactive oxygen species levels, augmentation of lipid oxidation extent, hyperpolarization of membrane potential, and reduction in intracellular ATP concentration. In addition, OEO reduced biofilm coverage on the surface of glass slides by 62.03 % compared with the untreated group. Meanwhile, OEO (1/8 MIC) treatment reduced the hemolytic activity of L. monocytogenes to 24.6 % compared with the positive control. Molecular docking suggested carvacrol and thymol might reduce the hemolytic activity of L. monocytogenes . The results of this study demonstrate that OEO exhibits inhibitory effects against L. monocytogenes , biofilms and LLO, which had potential as natural antimicrobial for the inhibition of L. monocytogenes .
研究领域:
食品安全
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微生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）