FOOD CHEMISTRY
·
2024
·
IF 8.5
Yihua Wei,Weihong Li,Yan Han,Yan Xiong,Yuanying Kuang,Jinyan Zhang
Zilpaterol hydrochloride (zilpaterol) is used in animal feed as it can increase the lean meat mass. However, consuming zilpaterol-containing animal products may damage human health. Therefore, rapid detection of zilpaterol is attracting increasing research attention. This study aimed to developed a fast, accurate, and ultrasensitive fluorescence immunoassay based on CdTe quantum dots (QDs). A CdTe QD fluorescence sensor was synthesized from thioglycolic acid using a simple hydrothermal method. The morphology and structure of the CdTe QDs were characterized using transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy. The detection limits of our method in swine urine and pork samples were 0.5 μg/L and 1.2 μg/kg, respectively. A wide linear range of 0.1–10000 μg/L (R 2 = 0.996) was achieved. Both within-run precision (CVw) and between-run precision (CV b ) were ≤ 10 %. The method was then successfully applied for the analysis of zilpaterol contents in swine urine and pork samples.
研究领域:
分析化学
·
食品安全
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Lulu Xu,Ruimei Wu,Xiang Geng,Xiaoyu Zhu,Yao Xiong,Tao Chen,Shirong Ai
Surface enhanced Raman spectroscopy (SERS) combined with rapid pretreatment technique was used to determine sulfonamide antibiotics (sulfadiazine and sulfathiazole) residue in swine urine. Au nanoparticles (AuNPs) were synthesized as Raman enhance substrate and the extraction of swine urine was purified with primary secondary amine (PSA), octadecyl silane (C 18 ) and graphitized carbon (GCB) to eliminate the interference of the matrix and different dosages of adsorbents (PSA, C 18 , GCB) were investigated. The results showed that the treatment with C 18 of 150 mg, GCB of 200 mg and PSA of 200 mg were an excellent approach for rapidly detecting sulfonamide antibiotics residue in swine urine. Combined with density functional theory calculation (DFT), Raman characteristic peaks of 819, 1102, 1173, 1588 cm −1 and 825, 1127 cm −1 were selected for qualitative and quantitative assessment of sulfadiazine and sulfathiazole in swine urine, respectively. Based on raman characteristic peak of 819 cm −1 , a good linear relationship between sulfadiazine concentration and Raman intensity was developed with R 2 = 0.9912, and based on raman characteristic peak of 825 cm −1 , a good linear relationship between sulfathiazole concentration and Raman intensity was developed with R 2 = 0.9941. And recoveries for five unknown concentration samples predicted were 98.47 ∼ 105.18% with relative standard deviation (RSD) of 1.53% ∼ 5.18%. This study demonstrated that SERS coupled with a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method could be employed to rapidly examine the sulfonamide antibiotics residue in swine urine towards its quality and safety monitoring.
研究领域:
食品安全
·
分析化学
·
兽医学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 21
·3966-3973
·
IF 3.614
Libin Wan,Haidong Gao,Xiao Liu,Shucai Gao,Li Zhou,Fayun Wang,Mantang Chen
The illegal use of clenbuterol seriously endangers food safety and human health. Accurate monitoring of the illegal use of clenbuterol in livestock can efficiently prevent the clenbuterol residue pork products from entering the consumer market. Thus, in this study, a simple, rapid, and sensitive method for the determination of clenbuterol in swine urine was developed using electromembrane extraction combined with liquid chromatography-tandem mass spectrometry. It should be noted that the electromembrane extraction method presented many advantages of simple operation, fast mass transfer rate, good sample clean-up capability, and less organic solvent consumption. The effect of important factors on the extraction efficiency of clenbuterol was investigated. Under the optimal conditions, good linearity was achieved for clenbuterol over the range of 1–1000 ng/ml (linear correlation [ R 2 ] = 0.9996). The recoveries of clenbuterol in swine urine at three spiked levels ranged from 83.7% to 110.0% with relative standard deviation values lower than 9.7% ( n = 4). The limits of detection and quantification for clenbuterol were 0.07 and 0.25 ng/ml, respectively. These results suggested that the proposed method has great potential for the extraction and determination of trace analyte in a complex sample matrix for monitoring illegal use in livestock.
研究领域:
分析化学
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食品科学
·
畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Xufeng Hao,Weixuan Liu,Yajun Zhang,Weijun Kang,Lingmei Niu,Lianfeng Ai
Chlorpromazine hydrochloride (CPZ) is a phenothiazine antipsychotic drug, which can effectively relieve the symptoms of chronic or severe mental disorders and reduce the manic phase of manic depression. However, excessive CPZ (more than 2 g per day) can cause serious harm to the health of the body, such as cholestatic jaundice, leukopenia, orthostatic hypotension, extrapyramidal effects and contact dermatitis. Therefore, a simple, fast, and accurate method to detect and identify CPZ is very important, especially for the detection of biological samples. In this study, based on the principle of inorganic salt inducing nanoparticle agglomeration, we synthesized silver colloids (AgNPs) with plasmon resonance effect. And we introduced inorganic salt MgBr 2 to induce self-limiting aggregation of AgNPs, resulting in a large number of “hot spots” in the nanoparticle gap. Thereby significantly enhancing the SERS intensity. The surface-enhanced Raman spectroscopy (SERS) method proposed in this paper can achieve real-time rapid detection of chlorpromazine hydrochloride in the field, with a linear range of 0.1–100 ppm (R 2 = 0.992). The calculated detection limit was 0.048 ppm. This strategy enables high stability and good repeatability of the SERS spectra. The application of established method can achieve the rapid and accurate determination of CPZ in pig urine. The recovery of standard addition was 87.50–107.70% and the RSD was between 2.87% and 7.42%. Therefore, the SERS detection technology in this study provides a new insight for the rapid, effective and accurate determination and identification of CPZ in pig urine.
研究领域:
分析化学
·
药物分析
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Min Feng,Steven Suryoprabowo,Hong Tao,Liqiang Liu,Qiankun Zheng,Hua Kuang
Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO.
研究领域:
兽医学
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分析化学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
Environmental Science-Nano
·
2018
·Vol. 5
·期 12
·2978-2992
·
IF 6.087
Dongfang Wang,Zhangyu Dai,Xian Shu,Po Bian,Lifang Wu,Dongqing Cai,Zhengyan Wu
In this work, amido-functionalized Fe3O4 microspheres (AFFMs) were loaded in network-structured MnO2 to form a nanocomposite named as OAM. OAM could remove chlortetracycline hydrochloride (CTC) and trivalent arsenic (As(III)) simultaneously from swine urine aqueous solution and soil. Therein, OAM has a high adsorption capability for CTC through hydrogen bonds. Additionally, OAM could effectively adsorb As(III) through electrostatic attraction and then oxidize it to pentavalent arsenic (As(V)). Afterward, the resulting OAM/CTC/As could be conveniently removed with a magnet. When OAM was deposited on the surface of a piece of cotton fabric (CF), the obtained OAM/CF could efficiently control the migration of CTC and As(III) in a sand–soil mixture. Importantly, OAM could effectively reduce the uptake of CTC and As by zebrafish, water spinach, and pakchoi, and promote the growth of water spinach. Finally, OAM possessed good biosafety for embryos of Caenorhabditis elegans. Thus, this work provides a new method to remove CTC and As(III) simultaneously in swine urine aqueous solution and soil, which may have a high application value.
研究领域:
环境科学
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材料科学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-8
·
IF 5.705
Peng Tao,Wang Jianyi,Zhao Sijun,Xie Sanlei,Yao Kai,Zheng Pimiao,Wang Sihan,Ke Yuebin,Jiang Haiyang
Organic/inorganic hybrid nanoflowers were synthesized from calcium phosphate and protein modified fluorescent gold nanoclusters and antigens. These nanoflowers are shown to be well suited labels for bioassay because they fulfill the functions of biological recognition and signal output. A fluorometric immunoassay was developed that was combined with immunomagnetic separation. In the detection system, the red fluorescence of the supernatant (measured at excitation/emission wavelengths of 360/640 nm) is found to be proportional to the clenbuterol (Clen) concentration after two immunomagnetic separations. The assay has a linear response in the 0.5 μg L −1 to 40 μg L −1 Clen concentration range, and 0.167 μg L −1 limit of detection. This makes it well suited for food safety monitoring. The average recoveries from spiked samples range from 92.7 to 109.1% (intra-assay) and 101.2 to 125.7% (inter-assay) with relative standard deviations of <11.6%. Spiked swine urine samples were analyzed by this method, and the results correlated well with data obtained by LC-MS/MS.
Fluorescent hybrid nanoflowers were fabricated with gold nanoclusters (BSA-AuNCs) and antigens. A fluorometric immunoassay based on the use of such nanoflowers and based on immunomagnetic separation was developed to detect clenbuterol residues in swine urine with satisfactory recoveries and acceptable accuracy.
研究领域:
分析化学
·
纳米技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2018
·Vol. 34
·期 1
·98-105
·
IF 1.671
Wan Qiu Xia,Hui Cai Zhang,Geng Nan Wang,Jing Liu,Jian Ping Wang
The residues of phenothiazines and benzodiazepines in foods of animal origin are dangerous to consumers. For inspection of their abuses, this study for the first time reported on the use of a chemiluminescence array sensor for the simultaneous determination of four phenothiazines and five benzodiazepines in pig urine. Two molecularly imprinted polymers were coated in different wells of a conventional 96-well microtiter plate as the recognition reagents. After sample loading, the absorbed analytes were initiated directly by using an imidazole enhanced bis(2,4,6-trichlorophenyl)oxalate–hydrogen peroxide system to emit light. The assay process consisted of only one sample-loading step prior to data acquisition, so one test was finished within 10 min. The limits of detection for the nine drugs in the pig urine were in a range of 0.1 to 0.6 pg/mL, and the recoveries from the fortified blank urine samples were in a range of 80.3 to 95%. Furthermore, the sensor could be reused six times. Therefore, this sensor could be used as a simple, rapid, sensitive and reusable tool for routine screening for residues of phenothiazines and benzodiazepines in pig urine.
研究领域:
分析化学
·
食品科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Jie Zhou,Kui Zhu,Fei Xu,Wenjun Wang,Haiyang Jiang,Zhanhui Wang,Shuangyang Ding
The residue of lincomycin (LIN) in edible animal foodstuffs caused by the widespread use of veterinary drugs is in need of rapid, simple, and sensitive detection methods. The present work introduces a fluorescent microsphere immunoassay (FMIA) for detecting LIN in different samples based on the competitive immunoreaction on the chromatography test strip. The residues of LIN in different samples compete with bovine serum albumin (BSA) labeled LIN conjugates on the T-line to bind to the anti-LIN monoclonal antibody labeled fluorescent microspheres (FM–mAbs). Captured FM–mAbs on the T-line represent the fluorescent intensity, which is detected under UV light and quantified by a fluorescent reader. Under optimized conditions, the dynamic range is from 1.35 to 3.57 ng/mL, and the 50% inhibition concentration (IC50) is 2.20 ng/mL. This method has 4.4% cross-reactivity with clindamycin and negligible cross-reactivity (<0.1%) with other analogues. To reduce the matrix effects, a dilution method is used to pretreat the samples, and the recoveries range from 73.92 to 120.50% with coefficient of variations <21.76%. In comparison with the results of ELISA and colloidal gold immunoassay, FMIA has obvious advantages such as easy operation, time savings, high sensitivity and specificity, and broader prospect.
研究领域:
食品科学
·
分析化学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2019
·
IF 1.248
Ming Xin Xu,Wei Li Zhao,Jing Liu,Tong He,Jing Jie Huang,Jian Ping Wang
Isoprenaline was used as the template to synthesize a molecularly imprinted polymer that was able to simultaneously recognize ten β-agonists. The simulation showed that the three-dimensional conformation of the template was the main factor responsible for the polymer’s recognition. The polymer particles were coated in the wells of a conventional microplate as the recognition reagent to prepare a chemiluminescence sensor. The light signal was initiated with a highly effective bis(2,4,6-trichlorophenyl) oxalate-H2O2-imidazole system. The assay contained only one sample-loading step followed by immediate data acquisition, so one measurement was complete within 12 min. The sensor was used to determine the ten analytes in porcine urine. The results showed that the senor achieved ultrahigh sensitivity, with limits of detection from 2.0 to 7.0 pg/mL, high recoveries from 78.6% to 99.4%, and satisfactory recycle performance up to seven times. This is the first study reporting a molecularly imprinted polymer based microtiter chemiluminescence sensor for the determination of β-agonists.
研究领域:
分析化学
·
环境科学
·
兽医学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Tao Peng,Jianyi Wang,Sijun Zhao,Yuyang Zeng,Pimiao Zheng,Demei Liang,Ghulam Mujtaba Mari,Haiyang Jiang
A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l -arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor . The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment , qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L −1 . In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L −1 , respectively, with detection limits of 0.003 and 0.023 μg L −1 . Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes , and single-analyte detection.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Wenliang Ge,Steven Suryoprabowo,Xiaoling Wu,Qiankun Zheng,Hua Kuang
The misuse of β-agonists constitutes a potential risk to public health and β-agonists has been forbidden in many countries. In this study, we describe an immunochromatographic test strip method for the rapid, sensitive, and inexpensive detection of mabuterol (MAB) and mapenterol (MAP) by cross-reactivity in pig urine. For the immunochromatographic test, the coating antigen which was obtained by conjugating the salbutamol to bovine serum albumin and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the control line and test line. The test was developed with a sensitive monoclonal antibody specific for MAB, which was generated by immunizing BALB/c mice with a well-characterized MAB- keyhole limpet haemocyanin conjugate, produced in our laboratory. Under optimized conditions, the cut-off limits of the semi-quantitative test strip for MBA and MPA were as low as 10 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. Our data indicate that the immunochromatographic assay allows provide sensitive, rapid, and specific on-site screening for MAB and MAP.
研究领域:
兽医学
·
分析化学
·
公共卫生
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）