MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Wenjing Wang,Yumin He,Suxiang He,Xiaoying Liu,Qing-wen Gui,Lei Deng,Hui Wang,Zhong Cao,Zemeng Feng,Benhai Xiong,Yulong Yin
L - Tryptophan (L-Trp), one of the essential amino acids, is crucial for human physiological homeostasis and a limiting amino acid in animal feed. In addition, L-Trp is a precursor of some important biomolecules in the body, such as pentraxin and melatonin. Disorders of L-Trp metabolism in the body may cause Alzheimer's or Parkinson's disease. Therefore, monitoring L-Trp levels in the body fluids quickly and accurately is essential. A colorimetric biosensor was developed for the rapid detection of L-Trp in solutions by coupling a novel, screened, and validated peptide aptamer with Au nanoparticles via the Au-S bond. The biosensor showed a wide linear detection range of 1 µM − 1000 µM, and in addition, it was simple to construct, and the reaction time was as short as 10 s. In the analysis of L-Trp in actual porcine serum samples, the relative standard deviations were 4.52 % to 3.01 % compared with those of the high-performance liquid chromatography method, and the spiked recoveries were 99.2 % to 100.2 %. This new type of recognition probe is likely to gain attention in the field of bioanalysis owing to its biocompatibility, ease of modification, and easy linkage to nanomaterials.
研究领域:
生物化学
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分析化学
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纳米技术
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产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 11
·4751-4759
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IF 4.813
Chen Dongjie,Wang Di,Wang Caixia,Wei Fang,Zhao Hongyuan,Lin Xiangmei,Wu Shaoqiang
Abstract Infection with African swine fever virus (ASFV) causes an acute and highly lethal hemorrhagic disease that has been responsible for huge economic losses in China. To exactly detect the antigen of ASFV, we established a rapid, no-wash, one-step sandwich-type immunoassay based on the amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) using two monoclonal antibodies (mAbs) M-5 and M-6 against ASFV p72. ASFV p72 in samples was captured by biotinylated mAb M-5 connected to the donor bead surface via streptavidin and “sandwiched” by mAb M-6 which was coated onto the acceptor bead. Efficacy and sensitivity trials revealed that the AlphaLISA could detect ≥0.78 ng/ml of purified p72 and with a linear range of 0.78–100 ng/ml. The AlphaLISA was specific for ASFV and did not cross-react with other common pathogenic porcine viruses. Compared with RealPCR ASFV DNA test and ASFV antigen detection kit, the sensitivity of the AlphaLISA evaluated in 60 porcine serum samples was 93% and 100%, respectively. The specificity was 100% and 91.7%, respectively. This study presents a good laboratory diagnostic tool for sensitive and efficient detection of ASFV in porcine serum. Key points • MAbs M-5 and M-6 recognized various epitopes of ASFV p72. • The established ASFV p72 AlphaLISA showed well specificity, high sensitivity, and satisfied correlation coefficient.
研究领域:
兽医学
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分子诊断学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Nutrition
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2022
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IF 5.285
Chizhu Ding,Xiang Chen,Xiaoyu Chen,Yue Liu,Menglin Xia,Ziyi He,Qinshu Kang,Xianghua Yan
Lysine is one of the essential amino acids and plays a vital role in the growth, development and health of pigs. Blood lysine concentration is a direct indication of lysine status; however, current methods can not satisfy the demands for rapid and on-site lysine concentration measurement of swine serum. Here, we developed blue-emissive nitrogen-doped carbon dots as a fluorescence probe for the determination of lysine with high fluorescence quantum yield, stability, sensitivity and specificity. The carbon dots were entrapped within hydrogel microstructures to fabricate microfluidic chips for rapid assay for lysine quantification. We further developed an imaging attachment to integrate the microfluidic chip and a smartphone into a portable point-of-care testing platform. This platform requires only 3 μL sample and has a linear detection range of 25 to 300 μmol/L with a limit of detection less than 16 μmol/L, which covers the normal range of lysine concentration in swine serum. We tested lysine concentration in swine serum using this platform with high accuracy, low sample consumption, and within 3 min. Together, these results may provide a rapid and portable platform for dynamic monitoring of swine lysine status and contribute to precise feed formula modulation with low-protein diet strategy.
研究领域:
生物化学
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分析化学
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畜牧学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Qin Zhu,Chu Liu,Li Zhou,Ling Wu,Kejun Bian,Julan Zeng,Jianxiu Wang,Zemeng Feng,Yulong Yin,Zhong Cao
Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity . In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm. The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr). Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08–1.0 μM) with a detection limit of 4.9 nM. No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity. The electrode also had good repeatability, reproducibility and stability. Compared with a commercial instrument analytical method, HPLC , the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%–102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.
研究领域:
生物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 4
·047505
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IF 3.9
Yao Xiong,Ruimei Wu,Lulu Xu,Ying Zhong,Yu Ge,Yangping Wen,Hang Yao,Weiqi Zhou,Shirong Ai
Extraordinary electronic performance and unique structural characteristic of black phosphorene (BP) often is used as electrode modified materials in electrochemical sensors. In this paper, a machine learning (ML) strategy for phosphorene nanozyme sensor and its the intelligent of clenbuterol (CLB) in pork and pig serum samples is prepared. The silver nanoparticles decorate BP to prevent oxidative degradation of BP surface and further hybridize with multi-walled carbon nanotubes (MWCNTs) composites containing nafion (Nf) treated with isopropanol (IP) to improve environmental stability and electrocatalytic capacity of BP. Back-propagation artificial neural network (BP-ANN) model combined with genetic algorithm (GA) is employed to optimize sensor parameters such as BP concentrations, MWCNTs concentrations and ratio of VNf:VIP, and compared with orthogonal experimental design (OED). Least square support vector machine, radial basis function and extreme learning machine are implemented to establish quantitative analysis model for CLB. The results showed that the CLB response current of BP sensor by BP-ANN-GA was improved 9.02% over OED method. Compared with the traditional linear regression, three models displayed better predictive performance, and LS-SVM was the best with the R2, RMSE and MAE and RPD of 0.9977, 0.0303, 0.0225, and 18.74, respectively. The average recoveries of CLB in pork and pig serum was 98.66% ∼ 101.67%, and its relative standard deviations was 0.19% ∼ 0.84%, indicating that electrochemical sensor using machine learning for intelligent analysis of CLB in animal-derived agro-products products was both feasible and practical.
研究领域:
分析化学
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食品科学
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生物医学工程
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产品关联
DOI
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2024
·Vol. 38
·期 15
·e9769
·
IF 1.8
Liwei Xu,Andong Zha,Xia Xiong,Dehui Sun
Rationale Succinic acid and lactic acid have been associated with diarrhea in weaned piglets. The level of succinic acid and lactic acid in serum, meat, and intestinal contents is important to elucidate the mechanism of diarrhea in weaned piglets. Methods A facile method was developed for the quantification of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC/MS/MS). The serum samples underwent protein precipitation with methanol. The meat and intestinal contents were freeze-dried and homogenized using a tissue grinding apparatus. Methanol–water mixture (80:20, v/v) was used for homogenizing the meat, while water was used for homogenizing the intestinal contents. An additional step of protein precipitation with acetonitrile was required for the intestinal contents. The resulting solution was diluted with water before being analyzed by UHPLC/MS/MS. Separation of succinic acid and lactic acid could be achieved within 3 min using a Kinetic XB-C 18 column. Results The coefficients of variation for peak areas of succinic acid and lactic acid were less than 5.0%. The established method demonstrated good linearity as indicated by correlation coefficients exceeding 0.996. Additionally, satisfactory recoveries ranging from 88.58% to 108.8% were obtained. The detection limits ( R S/N = 3) for succinic acid and lactic acid were determined to be 0.75 ng/mL and 0.02 μg/mL, respectively. Conclusion This method exhibited high sensitivity, simplicity in operation, and small sample weight, making it suitable for quantitative determination of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat. The method developed will provide valuable technical support in studying the metabolic mechanisms of succinic acid and lactic acid in pigs.
研究领域:
动物营养学
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兽医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FASEB JOURNAL
·
2021
·Vol. 35
·期 7
·e21497
·
IF 5.192
Sha Tu,Yanzhi Jiang,Haihua Cheng,Xiangning Yuan,Ying He,Yu Peng,Xiongqun Peng,Zhangzhe Peng,Lijian Tao,Huixiang Yang
Despite the increasing understanding of the pathophysiology of hepatic fibrosis, the therapies to combat it remain inadequate. Fluorofenidone (AKF-PD) is a novel pyridone agent able to ameliorate hepatic fibrosis in an experimental hepatic fibrosis model induced by dimethylnitrosamine. However, the underlying mechanism remains to be further elucidated. In light of the critical role of the NF-κB pathway in inflammation and hepatic fibrosis, together with the preliminary finding that AKF-PD decreases the release of proinflammatory cytokines in the endotoxemia and unilateral ureteral occlusion model, the aim of this study was to explore whether AKF-PD exerts an antifibrotic effect in hepatic fibrosis by inhibiting inflammation and suppressing the activation of the NF-κB pathway in vivo and in vitro. To test this possibility, the effect of AKF-PD on hepatic fibrosis models induced by both carbon tetrachloride (CCL 4 ) and porcine serum (PS) was investigated. Our results showed that AKF-PD treatment ameliorated hepatic injury and fibrosis in both models. Furthermore, the administration of AKF-PD induced a robust anti-inflammatory reaction revealed by the downregulation of the proinflammatory cytokines as well as the suppression of the infiltration of inflammatory cells in the fibrotic liver. The analysis of the mechanism of action demonstrated that the attenuation of the production of proinflammatory cytokines and chemokines mediated by AKF-PD in vivo and in vitro were accompanied by the suppression in the activation of the NF-κB signaling pathway. In conclusion, AKF-PD might be considered as an antifibrotic agent attenuating hepatic inflammation and fibrosis potentially through the suppression of the NF-κB pathway.
研究领域:
药理学
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肝病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Progress in Natural Science-Materials International
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2022
·
IF 4.269
Xue Li,Jinhua Li,Wentao Yang,Dandan Han,Na Yao,Hongbin Zhao,Xueying Chu,Xuemei Liang,Chunguang Bi,Chunfeng Wang,Guilian Yang
An ultrasensitive and selective sandwich-type fluorescent immunosensor based on fluorescence resonance energy transfer (FRET) between CdSe/ZnS quantum dots (QDs) and Au nanorods (AuNRs) was developed for the rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV). Modified CdSe/ZnS QDs and AuNRs were bioconjugated with primary antibodies M and GP5, respectively. When the target antigen was present, a well-known sandwich-type form CdSe/ZnS QDs-M antibody-antigen-GP5 antibody-AuNRs was produced, and thus reducing the distance between the QDs and NRs. As a result, FRET occurred and the fluorescence intensity of CdSe/ZnS QDs decreased, and this decrease was used to determine the antigen concentration. Ultrasensitive detection of PRRSV at low concentrations in swine serum was achieved with a limit of detection of 0.55 TCID 50 /mL and a linear detection range of 10 1 –3.5 × 10 4 TCID 50 /mL. Therefore, the fluorescent immunosensor is selective and highly sensitive; specifically, it can detect PRRSV at low concentrations in swine serum over a large concentration range. This proposed detection method can facilitate the development of high-performance fluorescent immunosensors for the detection of PRRSV and other antigens.
研究领域:
生物传感器
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纳米技术
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病毒学
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兽医学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yuehai Li,Jiabai Cai,Fengjiao Liu,Hui Yang,Ye Lin,Shunxing Li,Xuguang Huang,Luxiu Lin
Using chloroauric acid as precursor and β-cyclodextrin (β-CD) as reducing agent and stabilizer, β-CD@AuNPs with negative charge were synthesized by one-step colloidal synthesis method. The positive charged carbon quantum dots (CQDs) were synthesized by one-step of sonication of cetylpyridinium chloride . Under the role of static electricity, the fluorescence resonance energy transfer (FRET) occurred between CQDs and β-CD@AuNPs. CQDs and β-CD@AuNPs served as the fluorescence energy donors and receptors, respectively, i.e., the fluorescence of CQDs was turned off by β-CD@AuNPs. Based on the specific host-guest recognition between the inner cavity of β-CD and cholesterol, CQDs was replaced by cholesterol, the FRET could be interrupted, and then the fluorescence of CQDs was turned on. A good linear relationship between cholesterol concentration (10–210 μmol L −1 ) and fluorescence intensity was obtained and the LOD was 343.48 nmol L −1 . Because of excellent fluorescence quenching ability of FRET, the analytical performance (including LOD and linear scope) of such a turn off-on fluorescent nanosensor (e.g., CQDs/β-CD@AuNPs) was better than nanosensor only via competitive host-guest recognition (e.g., β-CD functionalized CQDs). The synergistic effect of competitive host-guest recognition and FRET was proved. Because of selective recognition, ultrasensitive, wide linear range, and strong anti-interference ability, CQDs/β-CD@AuNPs as a turn off-on fluorescent nanosensor was developed to determine cholesterol in porcine serum.
研究领域:
分析化学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yao Yu,Xiaolei Liu,Ning Xu,Lin Li,Yaming Yang,Mingyuan Liu,Lianjing Zhao,Xue Bai
Immunoassays are the primary method used for detection of foodborne parasitic infection. The nanomaterials used in these assays often present challenges such as complex preparation requirements and low stability during long-term storage and transport. Herein, based on cost-effective K 3 [Fe(CN) 6 ]-mediated in-situ generated CuFe Prussian blue analogues (CuFe-PBA) and quinoxaline derivatives with high fluorescence, a dual-mode immunoassay was proposed for foodborne parasitic infection. This platform is relied on the restoration of K 3 [Fe(CN) 6 ] by ascorbic acid (AA). In addition to this process, K 4 [Fe(CN) 6 ]-triggered the generation of CuFe-PBA, and the enhanced fluorogenic reaction of dehydrogenated AA and o -phenylenediamine (OPD) occur sequentially. Without complex probes preparation, the assay enabled the rapid on-site detection of ALP and foodborne parasitic infection through a colorimetric mode accessible via smartphone and offered enhanced sensitivity through a fluorescent mode. The method was used for the detection of ALP, achieving detection limits of 0.38 U/L and 0.03 U/L for colorimetric and fluorescence modes, respectively. Subsequently, the dual-mode immunoassay was applied to Trichinella spiralis infection. The sensitivity and specificity of both the colorimetric and fluorescence modes were 100 % in 950 pig serum samples. This immunosensor provides a universal analysis tool for the accurate, cost-effective and highly sensitive detection of foodborne parasitic infection.
研究领域:
分析化学
·
食品安全
·
生物技术
·
寄生虫学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）