Food Bioscience
·
2022
·
IF 5.318
Ping Yan,Jiang-lan Yuan,Xu Kang,Jin-ying Lv,Xiao-cui Liu
High internal phase emulsions (HIPEs) stabilized by nanoparticles based on biomacromolecules are challenging issues in recent decade. Herein, a newly developed HIPE was investigated by using heat-denatured porcine plasma protein (PPP) nanoparticles at pH 6.5 as emulsifier, and its emulsifying stability could be significantly enhanced by compounding carrageenan (CG). In the miscible system, PPP and CG formed hybrid particles through non-covalent interaction, and the sizes and zeta-potentials of the particles increased significantly along with addition of CG (from 0 to 0.7%, w/v), reached up to about 3.6 μm and −53 mV at 0.5% (w/v), respectively. CG weakened the ability of PPP to lower interfacial tension of oil/water (O/W), but increased the apparent viscosity of the system. The results from CLSM , rheology and stability experiments indicated a significant increasing trend of the HIPEs stability and solid-like characteristics along with addition of CG. Compared with the controls including bovine serum albumin (BSA), BSA-CG and CG alone, PPP-CG hybrid particles had good performance in fabricating and stabilizing the HIPEs. The work revealed the novel function of PPP as emulsifier of HIPEs and so offered the theoretical direction for application of PPP as a mass by-product, as well as an excellent HIPEs system for food , medicine and cosmetics fields.
研究领域:
食品科学
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生物材料
·
化学工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Wenjing Wang,Yumin He,Suxiang He,Xiaoying Liu,Qing-wen Gui,Lei Deng,Hui Wang,Zhong Cao,Zemeng Feng,Benhai Xiong,Yulong Yin
L - Tryptophan (L-Trp), one of the essential amino acids, is crucial for human physiological homeostasis and a limiting amino acid in animal feed. In addition, L-Trp is a precursor of some important biomolecules in the body, such as pentraxin and melatonin. Disorders of L-Trp metabolism in the body may cause Alzheimer's or Parkinson's disease. Therefore, monitoring L-Trp levels in the body fluids quickly and accurately is essential. A colorimetric biosensor was developed for the rapid detection of L-Trp in solutions by coupling a novel, screened, and validated peptide aptamer with Au nanoparticles via the Au-S bond. The biosensor showed a wide linear detection range of 1 µM − 1000 µM, and in addition, it was simple to construct, and the reaction time was as short as 10 s. In the analysis of L-Trp in actual porcine serum samples, the relative standard deviations were 4.52 % to 3.01 % compared with those of the high-performance liquid chromatography method, and the spiked recoveries were 99.2 % to 100.2 %. This new type of recognition probe is likely to gain attention in the field of bioanalysis owing to its biocompatibility, ease of modification, and easy linkage to nanomaterials.
研究领域:
生物化学
·
分析化学
·
纳米技术
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产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 11
·4751-4759
·
IF 4.813
Chen Dongjie,Wang Di,Wang Caixia,Wei Fang,Zhao Hongyuan,Lin Xiangmei,Wu Shaoqiang
Abstract Infection with African swine fever virus (ASFV) causes an acute and highly lethal hemorrhagic disease that has been responsible for huge economic losses in China. To exactly detect the antigen of ASFV, we established a rapid, no-wash, one-step sandwich-type immunoassay based on the amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) using two monoclonal antibodies (mAbs) M-5 and M-6 against ASFV p72. ASFV p72 in samples was captured by biotinylated mAb M-5 connected to the donor bead surface via streptavidin and “sandwiched” by mAb M-6 which was coated onto the acceptor bead. Efficacy and sensitivity trials revealed that the AlphaLISA could detect ≥0.78 ng/ml of purified p72 and with a linear range of 0.78–100 ng/ml. The AlphaLISA was specific for ASFV and did not cross-react with other common pathogenic porcine viruses. Compared with RealPCR ASFV DNA test and ASFV antigen detection kit, the sensitivity of the AlphaLISA evaluated in 60 porcine serum samples was 93% and 100%, respectively. The specificity was 100% and 91.7%, respectively. This study presents a good laboratory diagnostic tool for sensitive and efficient detection of ASFV in porcine serum. Key points • MAbs M-5 and M-6 recognized various epitopes of ASFV p72. • The established ASFV p72 AlphaLISA showed well specificity, high sensitivity, and satisfied correlation coefficient.
研究领域:
兽医学
·
分子诊断学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2022
·
IF 5.285
Chizhu Ding,Xiang Chen,Xiaoyu Chen,Yue Liu,Menglin Xia,Ziyi He,Qinshu Kang,Xianghua Yan
Lysine is one of the essential amino acids and plays a vital role in the growth, development and health of pigs. Blood lysine concentration is a direct indication of lysine status; however, current methods can not satisfy the demands for rapid and on-site lysine concentration measurement of swine serum. Here, we developed blue-emissive nitrogen-doped carbon dots as a fluorescence probe for the determination of lysine with high fluorescence quantum yield, stability, sensitivity and specificity. The carbon dots were entrapped within hydrogel microstructures to fabricate microfluidic chips for rapid assay for lysine quantification. We further developed an imaging attachment to integrate the microfluidic chip and a smartphone into a portable point-of-care testing platform. This platform requires only 3 μL sample and has a linear detection range of 25 to 300 μmol/L with a limit of detection less than 16 μmol/L, which covers the normal range of lysine concentration in swine serum. We tested lysine concentration in swine serum using this platform with high accuracy, low sample consumption, and within 3 min. Together, these results may provide a rapid and portable platform for dynamic monitoring of swine lysine status and contribute to precise feed formula modulation with low-protein diet strategy.
研究领域:
生物化学
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分析化学
·
畜牧学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Qin Zhu,Chu Liu,Li Zhou,Ling Wu,Kejun Bian,Julan Zeng,Jianxiu Wang,Zemeng Feng,Yulong Yin,Zhong Cao
Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity . In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm. The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr). Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08–1.0 μM) with a detection limit of 4.9 nM. No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity. The electrode also had good repeatability, reproducibility and stability. Compared with a commercial instrument analytical method, HPLC , the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%–102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2022
·Vol. 12
·期 11
·1438
·
IF 4.501
Guolei Liu,Xue Li,Qing Wang,Kuizhao Sun,Chuping Lee,Yue Cao,Weimeng Si,Haoran Wei,Zhongfang Li,Fagang Wang
In the work, a pig-blood-derived mesoporous carbon (BC) was prepared as a novel Fe-N-C material for the electrochemical sensor to detect hydrogen peroxide. Because of the unique nanostructure of Fe-BCs with rough surface structure, hierarchical pores, and high graphitization degree, the Fe-BCs, as a kind of advanced electrode material, exhibited remarkable performance in electrocatalysis. The sensor based on Fe-BCs exhibited an extra-long range from c and a detection limit of 0.046 μM (S/N= 3). The synthesis of low-cost, advanced carbon-based electrode materials from environmentally friendly pig blood for electrochemical sensor construction is a promising approach.
研究领域:
生物医学工程
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材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 4
·047505
·
IF 3.9
Yao Xiong,Ruimei Wu,Lulu Xu,Ying Zhong,Yu Ge,Yangping Wen,Hang Yao,Weiqi Zhou,Shirong Ai
Extraordinary electronic performance and unique structural characteristic of black phosphorene (BP) often is used as electrode modified materials in electrochemical sensors. In this paper, a machine learning (ML) strategy for phosphorene nanozyme sensor and its the intelligent of clenbuterol (CLB) in pork and pig serum samples is prepared. The silver nanoparticles decorate BP to prevent oxidative degradation of BP surface and further hybridize with multi-walled carbon nanotubes (MWCNTs) composites containing nafion (Nf) treated with isopropanol (IP) to improve environmental stability and electrocatalytic capacity of BP. Back-propagation artificial neural network (BP-ANN) model combined with genetic algorithm (GA) is employed to optimize sensor parameters such as BP concentrations, MWCNTs concentrations and ratio of VNf:VIP, and compared with orthogonal experimental design (OED). Least square support vector machine, radial basis function and extreme learning machine are implemented to establish quantitative analysis model for CLB. The results showed that the CLB response current of BP sensor by BP-ANN-GA was improved 9.02% over OED method. Compared with the traditional linear regression, three models displayed better predictive performance, and LS-SVM was the best with the R2, RMSE and MAE and RPD of 0.9977, 0.0303, 0.0225, and 18.74, respectively. The average recoveries of CLB in pork and pig serum was 98.66% ∼ 101.67%, and its relative standard deviations was 0.19% ∼ 0.84%, indicating that electrochemical sensor using machine learning for intelligent analysis of CLB in animal-derived agro-products products was both feasible and practical.
研究领域:
分析化学
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食品科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2024
·Vol. 38
·期 15
·e9769
·
IF 1.8
Liwei Xu,Andong Zha,Xia Xiong,Dehui Sun
Rationale Succinic acid and lactic acid have been associated with diarrhea in weaned piglets. The level of succinic acid and lactic acid in serum, meat, and intestinal contents is important to elucidate the mechanism of diarrhea in weaned piglets. Methods A facile method was developed for the quantification of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC/MS/MS). The serum samples underwent protein precipitation with methanol. The meat and intestinal contents were freeze-dried and homogenized using a tissue grinding apparatus. Methanol–water mixture (80:20, v/v) was used for homogenizing the meat, while water was used for homogenizing the intestinal contents. An additional step of protein precipitation with acetonitrile was required for the intestinal contents. The resulting solution was diluted with water before being analyzed by UHPLC/MS/MS. Separation of succinic acid and lactic acid could be achieved within 3 min using a Kinetic XB-C 18 column. Results The coefficients of variation for peak areas of succinic acid and lactic acid were less than 5.0%. The established method demonstrated good linearity as indicated by correlation coefficients exceeding 0.996. Additionally, satisfactory recoveries ranging from 88.58% to 108.8% were obtained. The detection limits ( R S/N = 3) for succinic acid and lactic acid were determined to be 0.75 ng/mL and 0.02 μg/mL, respectively. Conclusion This method exhibited high sensitivity, simplicity in operation, and small sample weight, making it suitable for quantitative determination of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat. The method developed will provide valuable technical support in studying the metabolic mechanisms of succinic acid and lactic acid in pigs.
研究领域:
动物营养学
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兽医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FASEB JOURNAL
·
2021
·Vol. 35
·期 7
·e21497
·
IF 5.192
Sha Tu,Yanzhi Jiang,Haihua Cheng,Xiangning Yuan,Ying He,Yu Peng,Xiongqun Peng,Zhangzhe Peng,Lijian Tao,Huixiang Yang
Despite the increasing understanding of the pathophysiology of hepatic fibrosis, the therapies to combat it remain inadequate. Fluorofenidone (AKF-PD) is a novel pyridone agent able to ameliorate hepatic fibrosis in an experimental hepatic fibrosis model induced by dimethylnitrosamine. However, the underlying mechanism remains to be further elucidated. In light of the critical role of the NF-κB pathway in inflammation and hepatic fibrosis, together with the preliminary finding that AKF-PD decreases the release of proinflammatory cytokines in the endotoxemia and unilateral ureteral occlusion model, the aim of this study was to explore whether AKF-PD exerts an antifibrotic effect in hepatic fibrosis by inhibiting inflammation and suppressing the activation of the NF-κB pathway in vivo and in vitro. To test this possibility, the effect of AKF-PD on hepatic fibrosis models induced by both carbon tetrachloride (CCL 4 ) and porcine serum (PS) was investigated. Our results showed that AKF-PD treatment ameliorated hepatic injury and fibrosis in both models. Furthermore, the administration of AKF-PD induced a robust anti-inflammatory reaction revealed by the downregulation of the proinflammatory cytokines as well as the suppression of the infiltration of inflammatory cells in the fibrotic liver. The analysis of the mechanism of action demonstrated that the attenuation of the production of proinflammatory cytokines and chemokines mediated by AKF-PD in vivo and in vitro were accompanied by the suppression in the activation of the NF-κB signaling pathway. In conclusion, AKF-PD might be considered as an antifibrotic agent attenuating hepatic inflammation and fibrosis potentially through the suppression of the NF-κB pathway.
研究领域:
药理学
·
肝病学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2021
·
IF 3.463
Weiwei Zhou,Wenlong Zheng,Haofeng Lv,Qunyan Wang,Bin Liang,Junliang Li
The application of natural biostimulants is proposed as an interesting approach to ensure sustainable yields and product quality in agricultural and horticultural crops. Here, the effects of foliar application of a protein hydrolysate derived from pig blood (Ppb) on biomass production, phenolic biosynthesis, and the correlation between phenolic profiles and antioxidant activities in lettuce were investigated. The Ppb application significantly enhanced the phenolic content and antioxidant properties in two genotypes of hydroponically cultivated lettuce by increasing the gene expression of enzymes (PAL, CHS, F3H, DFR, F35H, and UFGT) involved in phenolic biosynthesis. However, these changes occurred in a genotype-dependent manner. The results of HPLC and correlation analyses implied that the most contributory phenolic compositions to the increased antioxidant activities in Ppb-treated lettuce were flavonoids and anthocyanins. Meanwhile, no changes were observed in shoot and root fresh weights following Ppb application. These results clearly suggest that Ppb application is a useful strategy to improve antioxidant activities of lettuce by enhancing the biosynthesis of unique phenolic compounds without reducing plant yield.
研究领域:
农业
·
园艺
·
植物生理学
·
分子生物学
✓ 已验证
DOI
高影响因子
Carbon Letters
·
2024
·
IF 5.5
Chen Jinghong,Xie Huan,Lin Yuyin,Tan Zhenxing,Zheng Mingchen,Yuan Yong,Zhou Lihua
Ibuprofen (IBU), a common pharmaceutical and personal care product (PPCP), is a pervasive water pollutant with adverse ecological and human health effects after transformation and accumulation. In this study, we synthesized Fe, N-doped carbon quantum dots (Fe, N-CQDs) using pig blood and FeCl 3 as a precursor via a one-step hydrothermal method. TEM, XRD, XPS, and UV–Vis were used to characterize the physical and chemical properties of Fe, N-CQDs. We investigated the feasibility of Fe, N-CQDs in activating peroxymonosulfate (PMS) for IBU degradation under visible light. The experimental results revealed that Fe in Fe, N-CQDs predominantly formed a stable complex through Fe–N and Fe-OH, with a high degree of graphitization and a sp 2 -hybridized graphitic phase conjugate structure. The Fe, N-CQDs/Light/PMS system exhibited strong activity, degrading over 87% of IBU, maintaining a wide pH range (3–10) adaptability. Notably, Fe, N-CQDs acted as visible-light catalysts, promoting Fe 3+ /Fe 2+ cycling and PMS activation, generating both free radicals (SO 4 •– , ·OH) and non-radicals ( 1 O 2 , h + ) to effectively degrade IBU. This study presents an innovative approach for the sustainable utilization of pig blood as a biomass precursor to synthesize Fe- and N-doped carbon materials. This study provides a new approach for the sustainable and value-added utilization of natural wastes and biomass precursors of Fe- and N-doped carbon materials, which can be used to treat pollutants in water while treating discarded pig blood. Graphical abstract
研究领域:
环境科学
·
材料科学
·
化学
·
水处理
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产品关联
DOI
高影响因子
Antibiotics-Basel
·
2025
·Vol. 14
·期 8
·824
·
IF 4.6
Pu Guo,Xuwen Liu,Xiaoyi Li,Awais Ihsan,Zhongyuan Wu,Shulin Fu,Chun Ye,Yinsheng Qiu,Xu Wang,Qirong Lu,Yu Liu
Background:Glaesserella parasuis(GPS) is a conditional pathogen that colonizes the upper respiratory tract in pigs and causes Glässer’s disease, resulting in high morbidity and mortality in piglets. GPS infection increases the vascular endothelial permeability, but the mechanism has not been fully elucidated. Luteolin (Lut) is a naturally occurring flavonoid found in plants such as vegetables, herbs, and fruits, but its potential to treat the increased vascular endothelial permeability caused by GPS infection has not been evaluated.Results:This study revealed that GPS infection induces increased vascular endothelial permeability in porcine iliac artery endothelial cells (PIECs) by increasing the gene expressions of tumor necrosis factor (TNF), interleukin 6 (IL-6), IL-8, and IL-1β, and by regulating F-actin cytoskeleton reorganization. Mechanistically, GPS infection or Cluster of differentiation 44 (CD44) overexpression significantly increased the expressions of vascular-endothelial-permeability-related proteins (CD44; vascular endothelial growth factor (VEGFA); matrixmetalloProteinase-3 (MMP-3); MMP-9; and SRC proto-oncogene, non-receptor tyrosine kinase (c-Src)) and increased the vascular endothelial permeability; these changes were alleviated by a Lut treatment or CD44 silencing in the PIECs.Conclusions:This study comprehensively illustrates the potential targets and molecular mechanism of Lut in alleviating the GPS-induced increase in vascular endothelial permeability. The CD44 pathway and Lut may be an effective target and antibiotic alternative, respectively, to prevent the increased vascular endothelial permeability caused by GPS.
研究领域:
兽医学
·
微生物学
·
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）