JOURNAL OF CHROMATOGRAPHY A
·
2020
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IF 4.049
Shuai Liu,Zhen Pan,Ying Liang,Feng Li,Michael C. Breadmore,Min Zhang
An on-site ion analyzer based on capillary electrophoresis with pressure-driven flow through injection and capacitively coupled contactless conductivity detection has been developed for field monitoring of cations and anions in environmental waters. Automated time-pressure based hydrodynamic injection provides stable pL-nL scale injection (RSD = 1.96%, n = 30). A mixture of 400 mM Bis-Tris, 400 mM MOPS and 2 mM 18-crown-6 is used as the background electrolyte to provide repeatable separations. A proprietary hydrophilic coated 25 μm id capillary is used to suppress the electroosmotic flow. Separations of anions (Cl - , NO 3 - , NO 2 - , SO 4 2- , F - and PO 4 3- ) and cations (NH 4 + , K + , Na + , Ca 2+ and Mg 2+ ) are achieved by switching the polarity of the high voltage power supply in two individual runs. Signal fluctuations caused by the temperature or viscosity changes in on-site monitoring are corrected by on-line introduction of internal standards. RSDs of the migration time and the corrected peak height over ~35 h and 350 analysis cycles are <4.06%. The LODs of inorganic ions are in the range of 2.1 μM (K + ) to 6.8 μM (PO 4 3- ). The feasibility for on-site water monitoring with this system has been validated by a standard Ion chromatography method with comparable results obtained.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ting Zheng,Xiaoming Jiang,Naiwen Li,Xia Jiang,Chao Liu,Jing-Juan Xu,Peng Wu
Escherichia coli O157:H7 ( E. coli O157:H7 ) is typically associated with food- and water-borne outbreaks, thus making continuous monitoring essential. Photoelectrochemical (PEC) detection, which harvests the unique photo-to-electric property of photoactive species, has emerged as an exciting tool for bioanalysis. However, the current attempts are mostly carried out in lab, and less explored in field. Here, a simple PEC aptasensor for E. coli O157:H7 was designed and optimized (LOD: 200 cfu/mL). To promote its field application, a portable PEC analyzer was further constructed with a miniaturized LED (3 V, 3 W) for excitation and a USB-type electrochemical workstation for detection. Meanwhile, a re-chargeable power bank (5 V, 20,000 mAh, originally used for cell phone charging) was explored to power the LED through a self-designed electrical circuit. In this manner, the PEC analyzer could work continuously for more than 12 h. To facilitate transportation or the portable PEC analyzer, all components were mounted into a suitcase (size: 42 × 34 × 17 cm 3 ; wight: ∼3.5 kg). To validate the applicability of the portable PEC analyzer, on-site detection of E. coli O157:H7 in an in-land river was carried out (> 8 h), demonstrating the bright potential of the portable PEC analyzer for field environmental monitoring.
研究领域:
生物分析
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环境监测
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食品与水安全
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产品关联
DOI
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Chaoli Tang,Dachao Fu,Rujing Wang,Xiaoyu Zhang,Liman Wei,Mingjun Li,Chengpan Li,Qiao Cao,Xiangyu Chen
On-site monitoring of phosphate concentration has attracted much attention recently for soil fertility management in agricultural products. This study proposes a portable and easy-operate electrochemical microfluidic system with integrated reagent reactions for on-site continuous monitoring of soil phosphate. Polydimethylsiloxane (PDMS) chips with channel layers and PDMS substrates embedded with screen-printed electrodes (SPEs) were prepared using 3-D printed molds. Reagents and samples were pumped into the chips at optimal flow rates of $100~\mu \text{L}$ min $^{-{1}}$ to automatically react, which were used for cyclic voltammetry (CV) electrochemical detection of analytes via carbon black nanoparticles (CBNPs) modified electrodes. After optimizing experimental conditions, including pH value, liquid flow rates, and voltammetry scan rates, the system achieved a linear phosphate concentration range of 5– $100~\mu \text{M}$ with a detection limit (LOD) of $4.2~\mu \text{M}$ ; moreover, the system exhibited excellent resistance to interference long lifespan and excellent phosphate recovery rate from 91.1% to 110.48%. This system has been successfully applied to continuous field phosphate determination in real soil environments. Results elucidated that the system has good practicality and great potential for fine nutrient management in the field.
研究领域:
农业科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yilin Zhan,Na Li,Tianyi Qin,Linhui Peng,Weihua Deng,Zhongyong Xu,Lei Wang,Immanuel David Charles,Bin Liu
Buprofezin (BUP) is an insect growth regulator widely used in agriculture to control hemipteran pests, particularly the melon aphid, Aphis gossypii , due to its efficiency and low toxicity. Although approved by the Chinese government, its maximum residue limit (MRL) in food is strictly regulated, and conventional techniques for detecting BUP have several limitations. Our study reports successful BUP detection using a supramolecular fluorescent probe DP@ALB, constructed with chalcone-based fluorescent dye DP and albumin as the host. The probe offers advantages such as low cost, visual signal output with high fluorescence color variation, rapid response, and high sensitivity. Additionally, portable test strips enable convenient on-site BUP detection and simplifying field monitoring of spiked real samples. The study achieves precise qualitative and quantitative BUP analysis in grape fruit, groundwater, and soil with satisfactory recoveries. Further, the biological applicability of sensor for the in vitro detection of BUP in L929 living cells was demonstrated. This research breakthrough overcomes the limitations of traditional analytical methods, offering an efficient and reliable approach for food and environmental monitoring and pesticide residue detection.
研究领域:
食品科学
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环境科学
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分析化学
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生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xu Zhao,Deshuai Kong,Rui Jin,Hongxia Li,Xu Yan,Fangmeng Liu,Peng Sun,Yuan Gao,Geyu Lu
The rapid and on-site monitoring of thiram is of great significance because the residue of thiram in agriculture has caused great contamination in environment and food, as well as threaten to the human health. Herein, we constructed a fluorometric strategy for on-site monitoring thiram via the aggregation-induced emission enhancement (AIEE) effect of glutathione-capped gold nanoclusters (GSH-AuNCs). The dissociated Ag + ions can effectively enhance the fluorescent emission intensity of GSH-AuNCs at 570 nm owing to the AIEE phenomenon. Upon the addition of thiram, the fluorescence enhancement effect was weakened due to the strong coordinative interaction between Ag + and thiram. By monitoring the signal change, the thiram residues were quantitatively analyzed with the detection limit of 0.05 μg mL −1 . Notably, a home-made electronic-eye (E-eye) system, containing of a test-strip and a smartphone, was constructed as a portable platform to recognize and quantitatively analyze thiram in 10 min on the basis of captured fluorescence signals from a test strip with immobilized probe by direct photographing, which provides a direct quantitative tool to identify pesticide. Taking advantage of user-friendliness, portability and simplicity, the E-eye system are expected to fulfill the demand for quantitative on-site testing of pesticide especially in resource-limited areas.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Changchang Li,Yue Zhi,Minnan Zhu,Chengyu Yang,Yayang Tian,Tao Chen,Yuan Zhao,Jie Gao,Linfeng Chen
The development of simple, efficient and low-cost methods for rapid on-site monitoring have always been a challenging topic in the field of analysis. Here, we successfully constructed a selective, sensitive and rapid colorimetric sensor for the sensing of diethylstilbestrol (DES) by using molecular imprinting technology to construct specific recognition sites on the surface of Fe-based metal organic frameworks (Fe-MOF) peroxidase-like nanozyme. The surface of obtained sensor (Fe-MOF@MIP) had abundant channels and imprinted cavities that allowed the substrate to enter and responded with Fe-MOF core to drive the chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). When DES was present, it could specifically enter the cavities and obstruct the channels of substrate into the Fe-MOF core, thereby inhibiting the chromogenic reaction. The experimental results demonstrate that the sensor had excellent selectivity, anti-interference and catalytic stability. In the linear range of 0 – 1.67 μ M, the detection limit is as low as 8.43 nM, which is superior to peer sensors. To better adapt to in-situ analysis, we have further developed a rapid analysis method based on photo colorimetry, which performed well in real sample analysis and recovery test with the results consistent with the gas chromatograph-mass spectrometer (GC–MS), demonstrating its great potential for rapid on-site monitoring.
研究领域:
分析化学
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生物医学工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
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IF 11.3
Wenjing Wang,Shenghong Li,Liumo Jiang,Xiaoju Cui,Yan Gao,Haijing Ning,Qinglian Li,Xiaolin Jiang,Mo Zhang,Dehui Deng,Yang Shu,Jianhua Wang,Yafeng Guan,Xuhui Geng
The susceptibility of sensors to sulfur poisoning is the biggest challenge when monitoring high concentrations of hydrogen sulfide (H 2 S). In order to solve this problem, a portable detection instrument based on electrochemical sensor was developed. The “chainmail” catalyst of graphene-encapsulated cobalt-nickel nanoparticles (CoNi@NGs) was synthesized and doped with conductive carbon black (CCB) as sensing material. The graphene shell could protect core metal from sulfur poisoning. The anti-sulfur poisoning performance of the developed instrument reached the best level in literature. After 60 days of intermittent detection of 100 ppm H 2 S or on-site monitoring in wastewater treatment tank, the signals only decreased by 1.3 % and 1.6 %, respectively. By doping CCB, a porous structure was constructed on the working electrode membrane (WEM), which reduced the response and recovery time by 87.9 % and 77.0 %, to 14 s and 24.5 s, respectively. In addition, the instrument had a wide detection range (20 ppb-700 ppm), high sensitivity (0.580 μA/ppm) and limit of detection (LOD) of 0.52 ppb (3σ). Finally, the instrument was applied to monitor of H 2 S in wastewater treatment tank and sewage sewer of an institute, which showed that the instrument had broad application prospects for on-site monitoring of H 2 S in real environments.
研究领域:
环境科学
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传感器技术
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuqing Qin,Weikang Ke,Aroosha Faheem,Yueyun Ye,Yonggang Hu
Due to growing food safety issues, developing economic, rapid, and sensitive strategies for food spoilage monitoring has attracted significant attention. Here, a Bacillus subtilis spore-based biosensor is presented for rapid, highly sensitive, visual biogenic amines detection. The biosensor is fabricated through biogenic amines-induced pH increase which inhibits the electron transfer between Cu ion sites within CotA-laccase on the spore surface, leading to decrease in catalytic oxidation activity towards the chromogenic substrates. The developed system integrated with smartphone analysis realized the on-site monitoring of histamine with a detection range of 0.17–120 mg L −1 , and a detection limit of 0.17 mg L −1 (3σ). Moreover, the color change induced by histamine is observable by the naked eye. The smart biosensor was successfully applied for food freshness evaluation in raw meat samples, showing several advantages, including eco-friendliness, low cost, and high stability, meeting the demands of on-site monitoring in the food safety field.
研究领域:
食品科学
·
生物技术
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jianxiong Chen,Mengyao Hao,Yu Xin,Rui Zhu,Zhenghua Gu,Liang Zhang,Xuan Guo
Designing efficient and rapid methods for the detection of organophosphorus pesticides (OPs) residue is a prerequisite to mitigate their negative health impacts. In this study, we propose the concept of an enzyme catalysis system-based hydrogel kit integrated with a smartphone detector for in-field screening of OPs. Here, we rapidly prepared phosphotriesterase hybrid nanoflowers (PTE-HNFs) using a self-assembly strategy by adding external energy and embedded the nanocomposite in sodium alginate (SA) hydrogel to construct a target-responsive hydrogel kit. The color response of the kit is induced by catalyzing methyl parathion (MP) to produce p-nitrophenol. For on-site quantification, the color variations of the portable kit are converted into digital information through a smartphone, which exhibits an applicable linear range towards OPs. The hydrogel sensing platform demonstrates a wide linear range (1–150 μM) and low detection limit (0.15 μM) for MP while maintaining high reliability, excellent long-term stability, and ease of operation. Overall, the PTE-HNFs-based SA hydrogel kit provides a useful strategy for simple and sensitive detection of MP and holds great potential for applications in detecting OPs in food and environmental water.
研究领域:
环境科学
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分析化学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2021
·
IF 5.565
Yanan Zheng,Liudan Wei,Linwei Duan,Fangfang Yang,Guixiang Huang,Tianyi Xiao,Min Wei,Yanling Liang,Huiting Yang,Zhipeng Li,Dan Wang
With increasing industrial activities, mercury has been largely discharged into environment and caused serious environmental problems. The growing level of mercury pollution has become a huge threat to human health due to its significant biotoxicity. Therefore, the simple and fast means for on-site monitoring discharged mercury pollution are highly necessary to protect human beings from its pernicious effects in time. Herein, a “turn off” fluorescent biosensor (mCherry L199C) for sensing Hg 2+ was successfully designed based on direct modification of the chromophore environment of fluorescent protein mCherry. For rapid screening and characterization, the designed variant of mCherry (mCherry L199C) was directly expressed on outer-membrane of Escherichia coli cells by cell surface display technique. The fluorescent biosensor was characterized to have favorable response to Hg 2+ at micromole level among other metal ions and over a broad pH range. Further, the cells of the fluorescent biosensor were encapsulated in alginate hydrogel to develop the cells-alginate hydrogel-based paper. The cells-alginate hydrogel-based paper could detect mercury pollution in 5 min with simple operation process and inexpensive equipment, and it could keep fluorescence and activity stable at 4 °C for 24 hr, which would be a high-throughput screening tool in preliminarily reporting the presence of mercury pollution in natural setting.
研究领域:
环境科学
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生物技术
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Huimin Lu,Miaosi Li,Azadeh Nilghaz,Lizi Li,Guangxue Chen,Yanbin Jiang,Junfei Tian
A low-cost and portable paper-based analytical device has been developed for high throughput and on-site monitoring TC residue in milk through visualized colorimetric reaction. The filtration and concentration effect induced by the porous nature of paper contribute to strengthen the color intensity, leading to quantitative and sensitive detection of tetracycline reaching 1 ppm detection limit, with the linear range of 1–100 ppm both in water and milk samples. The applicability was demonstrated by detection of TC in 18 different types of real milk samples with good recovery ranging from 88% to 113%. Furthermore, the dynamic degradation behavior of tetracycline was monitored through the device. To the best of our knowledge, this is the first report of colorimetric detection of tetracycline in milk using the paper-based device. This simple, fast, cost-effective (~$0.50 per device) and equipment-free paper-based platform provides a promising tool for future application in food and environmental safety.
研究领域:
分析化学
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食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-14
·
IF 5.3
Deng Tingliu,Wu Wuming,Zhou Jingjing,Zeng Qin,Wang Heye,Deng Chunyan
A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization. This allows for a high-density immobilization of bacteriophage particles, achieving approximately 71 pcs μm −2 . Remarkably, the bacteriophages immobilized MXene nanostructured electrodes still maintain their viability and functionality, ensuring their effectiveness in pathogen detection. Therefore, the proposed biosensor exhibited enhanced sensitivity with a low limit of detection (LOD) of 5 CFU mL −1 . Notably, the biosensor shows excellent specificity in the presence of other bacteria that commonly contaminate food and can distinguish live Salmonella from a mixed population. Furthermore, it is applicable in detecting live Salmonella in food samples, which highlights its potential in food safety monitoring. This biosensor offers simplicity, convenience, and suitability for resource-limited environments, making it a promising tool for on-site monitoring of foodborne pathogenic bacteria.
研究领域:
生物传感器
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食品科学
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电化学
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微生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）