COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Weifeng Lin,Guanglong Ma,Jiang Wu,Shengfu Chen
Poly(sulfobetaine methacrylate) (pSBMA) and poly(carboxybetaine methacrylate) (pCBMA) are two well-known zwitterionic polymers known for their excellent antifouling properties. In this work, these two zwitterionic polymers were compared both in vitro and in vivo . Both of them exhibited excellent antifouling properties and low macrophage uptake although there were negligible differences in resistance to nonspecific protein adsorption of their hydrogels and cell internalization of their star polymers. However, it is found that the β- Cyclodextrin-CBMA (CD-CBMA) showed a circulation time one order of magnitude longer than CD-SBMA, which implied that small differences in vitro may lead to a dramatic difference in vivo . This work demonstrated that pCBMA showed greater potential than pSBMA in biomedical applications.
研究领域:
生物材料学
·
医学工程
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Chunli Yu,Yao Hu,Yuqin Zhang,Wei Luo,Jing Zhang,Peilun Xu,Jun Qian,Jun Li,Jianfeng Yu,Jin Liu,Wenguang Zhou,Shengxi Shao
In industrial-scale cultivation of microalgae, salinity stress often stimulates high-value metabolites production but decreases biomass yield. In this research, we present an extraordinary response of Arthrospira platensis to salinity stress. Specifically, we observed a significant increase in both biomass production (2.58 g L −1 ) and phycocyanin (PC) content (22.31%), which were enhanced by 1.26-fold and 2.62-fold, respectively, compared to the control, upon exposure to exogenous glycine betaine (GB). The biochemical analysis reveals a significant enhancement in carbonic anhydrase activity and chlorophyll a level, concurrent with reductions in carbohydrate content and reactive oxygen species (ROS) levels. Further, transcriptomic profiling indicates a downregulation of genes associated with the tricarboxylic acid (TCA) cycle and an upregulation of genes linked to nitrogen assimilation, hinting at a rebalanced carbon/nitrogen metabolism favoring PC accumulation. This work thus presents a promising strategy for simultaneous enhancement of biomass production and PC content in A. platensis and expands our understanding of PC biosynthesis and salinity stress responses in A. platensis .
研究领域:
生物技术
·
分子生物学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Miao Wang,Jiang Yuan,Xiaobo Huang,Xianmei Cai,Li Li,Jian Shen
Grafting-from has proven to be a very effective way to create high grafting densities and well-controlled polymer chains on different kinds of surfaces. In this work, we aim to graft zwitterionic brush from cellulose membrane (CM) via ARGET-ATRP (Activator Regenerated by Electron Transfer ATRP) method indirectly for blood compatibility improvement. Characterization of the CM substrates before and after modification was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), water contact angle measurements, X-ray photoelectron spectroscopy analysis, and atomic force microscopy, respectively. The results demonstrated zwitterionic brushes were successfully grafted on the CM surfaces, and the content of the grafted layer increased gradually with the polymerization time. The platelet adhesion, hemolytic test and plasma protein adsorption results indicated the cellulose membrane had significantly excellent blood compatibility featured on lower platelet adhesion and protein adsorption without causing hemolysis. The functionalized cellulose substrate could have a great potential usage for biomedical applications.
研究领域:
生物材料学
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Medicinal Chemistry
·
2025
·
IF 3.6
Zhenyuan Wang,Mi Wang,Qingsheng Tao,Yufei Li,Hao Wang,Mei Zhang,Xueli Liu,Jiaheng Zhang
Salicylic acid (SA) is a natural lipophilic active ingredient commonly used in cosmetics and skin disease treatments, offering benefits such as exfoliation, anti-inflammation effects, antibacterial properties, oil control, and acne alleviation. However, its poor water solubility, low bioavailability, and potential side effects, such as allergies, irritation, and dryness, hinder its widespread application. In this study, we prepared a betaine–salicylic acid (BeSA) cocrystal and systematically characterized its crystal structure, biological activity, and clinical efficacy. The results showed that BeSA has significantly lower irritancy and cytotoxicity than SA, but exhibits excellent anti-inflammatory and antioxidant properties as well as high moisturizing and anti-acne efficacy, making it a potential alternative to SA. Further, quantum chemical calculations and molecular docking simulations were conducted to investigate the intrinsic mechanisms underlying the excellent bioactivity of BeSA cocrystals. This study introduces an innovative solution for safer and more effective skincare formulations based on SA and offers theoretical guidance regarding material engineering and further material optimization, which has crucial implications for both industry and academia.
研究领域:
化妆品科学
·
药物化学
·
材料工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Shaobo Zhang,Xinghui Yang,Bin Tang,Lingjun Yuan,Kui Wang,Xiangyu Liu,Xingli Zhu,Jianna Li,Zaochuan Ge,Shiguo Chen
We report a facile and environment-friendly finishing method for cotton fabrics using zwitterionic sulfopropylbetaine (ISB) and quaternary ammonium salt (IQAS) with reactive isocyanate group (NCO) as antimicrobial finishing agents via dipping–padding–drying process. The results showed that ISB and IQAS can be covalently bound onto the cotton fabric surface, imparting exellent antifouling activity and bactericidal activity, respectively. The dually finished cotton fabrics exhibited excellent bactericidal and antifouling performance without significantly affect their water vapor permeability and air permeability. All the finished cotton fabrics exhibited improved breaking strength, tearing strength, bursting strength and hydrophilicity compared to raw cotton fabrics. Atomic force microscope research results indicated that the adhesion force between bacterial and finished cotton fabric surfaces increased with the QAS content, and they can be tailored by changing the ISB/IQAS ratio. Most importantly, non-leaching antimicrobial and antifouling cotton fabrics did not induce skin stimulation and toxic effects to animals because ISB and IQAS were chemically bound to cotton fabrics, ensuring that our finishing method have great potential for industrial and healthcare applications, including but not limited to fabrics, surgical equipment water purification systems, packaging and public health settings.
研究领域:
材料科学
·
生物医学工程
·
纺织工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2013
·
IF 3.334
Chunying Luo,Xixi Wang,Ge Gao,Lian Wang,Yongxin Li,Chengjun Sun
The study systematically investigated free, conjugate and total phenolics (phenolic acids and flavonoids) in leaves of 19 Chinese and one American sweetpotato cultivars grown in China. Three extraction/hydrolytic methods (direct extraction and acidic and basic hydrolysis) for sample preparation were employed to obtain different forms of phenolics. Twenty-nine phenolics were separated and identified using HPLC–DAD and HPLC–ESI–MS/MS. Three quercetin glycosides were characterised for the first time from this plant. Contents of the principal phenolics identified were determined by the HPLC–DAD procedure, which was validated in terms of linearity, precision, accuracy and limit of detection and quantification. Moreover, to the best of our knowledge, it is the first to reveal and demonstrate artifacts of esterification during acidic methanolic and ethanolic hydrolysis, and chromatographic behaviours, UV spectra and MS data of 20 hydroxycinnamic acid methyl and ethyl esters were obtained using acidic methanolic and ethanolic hydrolysis.
研究领域:
植物化学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2012
·Vol. 5
·期 1
·59-63
·
IF 1.547
Suo Decheng,Li Lan,Zhang Su,Su Xiaoou
A simple, highly sensitive ion chromatographic method for the simultaneous determination of betaine, choline and carnitine in feed premixes is described. Premixes were extracted via ultrasonication in water for 20 min and extracts were analyzed by ion chromatography using an aqueous 3.0 mmol L−1 methane sulfonic acid solution containing 15% (v/v) acetonitrile as the eluent and an IonPac SCS1 column. The composition of the mobile phase was optimized for the efficient separation of the three main analytes from each other as well as from common inorganic cations and trimethylamine, which is an ingredient for the synthesis of the analytes. The recoveries of choline, carnitine and betaine spikes added to premixes were all greater than 90% with replicate relative standard deviations of less than 10%. The limits of detection of this method for these analytes in premixes were calculated to be 10, 6 and 20 mg kg−1, respectively.
研究领域:
分析化学
·
营养学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
·
2015
·Vol. 27
·期 6
·2313-2319
·
IF 2.559
Liang Jin-Jin,Li Yun-Hui,Liu Fei,Li Hong-Ye,Liu Jie-Sheng,Yang Wei-Dong
To find new chemical modifiers and new modified clays with excellent algae removal efficiency, the removal effects of betaine and five sulfobetaines including octyl sulfobetaine, caprylyl sulfobetaine, lauryl sulfobetaine, myristyl sulfobetaine, and palmityl sulfobetaine (SB3-16) against the harmful alga Alexandrium tamarense were observed. Betaine and the five sulfobetaines exhibited certain removal effects against A. tamarense with the highest removal efficiency by SB3-16. Hence, a series of modified clay, montmorillonite (MMT) intercalated by SB3-16 were prepared, and their removal efficiencies were evaluated. The organo-clay with 11 wt% of SB3-16 exhibited the highest removal efficiency against A. tamarense cells. Combining the environmentally benign nature of SB3-16 and the high efficiency of SB3-16 modified clay against A. tamarense , SB3-16 modified clay could be a favorable choice to control harmful algal blooms (HABs) of this species.
研究领域:
海洋生物学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2016
·
IF 3.187
Jiantao Chen,Xiangjun Gong,Chaoxi Zeng,Yonghua Wang,Guangzhao Zhang
It is known that urea can induce protein denaturation that can be inhibited by osmolytes. Yet, experimental explorations on this mechanism at the molecular level are still lacking. We have investigated the resistance of betaine to the urea-induced denaturation of lysozyme in aqueous solutions using low-field NMR. Our study demonstrates that urea molecules directly interact with lysozyme, leading to denaturation. However, betaine molecules interacting with urea more strongly than lysozyme can pull the bound urea molecules from lysozyme so that the protein is protected from denaturation. The number of urea molecules bound to a betaine molecule is given under different conditions. Proton NMR spectroscopy (1H-NMR) and Fourier transform infrared spectroscopy reveal that the interaction between betaine and urea is through hydrogen bonding.
研究领域:
生物物理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Guangxin Yuan,Yongbo Peng,Zhenghua Liu,Jieya Hong,Yao Xiao,Jialiang Guo,Norman W. Smith,Jacques Crommen,Zhengjin Jiang
In order to prepare zwitterionic HILIC monolithic columns with high polarity, the highly hydrophilic monomer N , N -dimethyl- N -acryloyloxyethyl- N -(3-sulfopropyl)ammonium betaine (SPDA) and crosslinker N , N ′-methylenebisacrylamide (MBA) were employed for developing a novel sulfoalkylbetaine type stationary phase. The polymerization parameters were systematically optimized in order to obtain a satisfactory performance for column permeability, mechanical stability, hydrophilicity, efficiency and selectivity. Compared to the previously reported poly( N , N -dimethyl- N -methacryloxyethyl- N -(3-sulfopropyl)ammonium betaine- co -ethylene dimethacrylate) (poly(SPE- co -EDMA)) monolith and the poly(SPDA- co -EDMA) monolith that we developed, a significantly enhanced hydrophilicity was obtained on the poly(SPDA- co -MBA) monolithic column, illustrated by the lowered critical composition of the mobile phase corresponding to the transition from the HILIC to the RP mode. Excellent permeability, reproducibility and stability were achieved on this optimized poly(SPDA- co -MBA) monolith. A column efficiency of 70,000 plates/m was obtained for the analysis of bases at a linear velocity of 1.95 mm/s. As expected, by studying the influence of mobile phase pH and salt concentration on their retention, a weak electrostatic repulsion interaction for negatively charged analytes was also observed at low organic solvent content on the poly(SPDA- co -MBA) monolithic column. The final optimized poly(SPDA- co -MBA) monolith exhibited good selectivity for a series of polar compounds, such as phenols, bases, benzoic acid derivatives, small peptides, urea and allantoin.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2025
·
IF 3.7
Feiyun Li,Huinan Liang,Helin Li,Yanjun Tang
The high cost of enzymatic glycolysis has seriously restricted the industrialization of lignocellulose-based sugar platform technology. Recovering and recycling cellulase can reduce the cost. Here, a thermo-responsive claw-type polysulfobetaine (PSPA) was constructed for hydrophobic grasping and efficient recycling of cellulase. Compared with the linear sulfobetaine homopolymer (PSPE), PSPA had more sensitive temperature response and strong cellulase recovery ability. PSPE-3 (Mw=355.1 kDa) was added to the hydrolysis system of corncob residue (CCR) (50 °C), at 0.4 mass ratio of PSPE-3 to cellulase, and after enzymatic hydrolysis, 50% of the cellulase was saved when cooling to 25 °C. But only 0.05 times PSPA-1 (Mw=30.0 kDa) was added to the CCR system, and 70% of the cellulase was saved when cooling to 25 °C. This work presents a claw shaped protein-capture agent, polysulfobetaine for capturing cellulase, which is of great significance to reduce the lignocellulosic hydrolysis cost, and efficiently separate enzyme proteins.
研究领域:
生物化学
·
工业酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 3
·454
·
IF 4.1
Huiying Xie,Jiajing Zeng,Wenli Feng,Wei Gao,Zhongxiong Lai,Shengcai Liu
Betalains differ from anthocyanins, and they cannot coexist in the same plant under natural conditions. The L-DOPA 4,5-dioxygenase encoded by theDODAgene is a key step in the pathway of betalain biosynthesis in Caryophyllales plants. Amaranth is an important resource for the study and extraction of betalains. In order to clarify the function ofAtrDODAfamily genes in betalain biosynthesis, we screened out threeAtrDODAfamily gene members associated with betalains based on a genome database and RNA-seq databases ofAmaranthus tricolor. Their characterization and expression pattern were further analyzed. The result of subcellular localization showed that all three AtrDODA members were located in the nucleus. Betacyanin and betaxanthin were promoted by paclobutrazol treatment in the leaves and stems of ‘Suxian No.1’ (red), while they were inhibited by gibberellin and darkness, which were consistent with the gene expression pattern ofAtrDODAs. After heterologous transformation of theAtrDODA1-1promoter into tobacco with GUS staining analysis, the promoter activity ofAtrDODA1-1of ‘Suxian No.1’ (red) amaranth was significantly higher than that of ‘Suxian No.2’ (green) amaranth. Furthermore, we analyzed the promoter activity ofAtrDODA1-1by GUS staining and qRT-PCR after sprayed exogenous MeJA and GA3on theAtrDODA1-1promoter transformed tobacco plants. The results showed thatAtrDODA1-1responded to plant hormones. This study could lay a foundation for revealing the biological functions of the amaranthDODAgene family, and provide new clues for the molecular mechanism of betalain synthesis.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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