Desalination and Water Treatment
·
2016
·
IF 1.272
Quan Zhang,Fei-yun Sun,Wen-yi Dong
A submerged membrane bioreactor (MBR) employed for nitrobenzene-contain water resource treatment was operated for investigation of system effectiveness and for specification of nitrobenzene (NB) removal pathway. Two sludge retention times (2 and 25 d) that regulated biomass content in MBR were adopted to evaluate the importance of activated sludge for NB removal through biodegradation way. From a long-term examination, both of organic biodegradation by activated sludge and rejection by fresh and/or fouled membrane were the major ways to remove organic matters including NB. However, activated sludge played a rather significant role in NB removal. A lengthening solid retention time form 2 to 25 d in MBR resulted in a significantly improvement of removal efficiency in dissolved organic matters, ammonia-N and NB, due mainly to the continuous accumulation of biomass within bulk. Especially, the NB removal efficiency displayed an apparently positive correlation with the biomass content. After sludge acclimation process, the MBR was able to remove NB by a removal rate at 1.266 mg/(gMLSS h), equivalent to the NB removal efficiency up to 80%. In addition, by Fluorescence excitation-emission matrix observation, it was found that biomass growth and accumulation could also impact the effective removal of organic fractions, as that there were obvious selectivity of organic removal by biodegradation of activated sludge. The experimental results obtained herein are very useful to recognize the refractory organics removal pathway in MBR, and to understand the importance of activated sludge.
研究领域:
环境科学
·
污水处理
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Mei Duan,Xianglong Pang,Fei Hao,Heng Liu,Xinxin Cui,Jingyi Duan,Wen Wang,Xiaoguang Li
Biofouling poses a notorious challenge during photosynthetic microorganism cultivation in photobioreactors (PBRs) by hindering light penetration, degrading the cultivation environment and eventually diminishing bioproductivity. To solve this problem, we developed a superhydrophobic SiO2 coating with antireflective properties using sol–gel technology. The coating is cost-effective due to its simple, fluorine-free, and heat-free fabrication process, showing excellent performance in both preventing cyanobacterial adhesion and enhancing light transmission. Compared to uncoated substrates, the transmittance increases by 5.8 to 7.0%, depending on the substrate material (glass, polystyrene, poly(vinyl chloride) and polycarbonate). When applied to glass tube PBRs, this coating significantly promotes cyanobacterial proliferation and chlorophyll-a content compared to uncoated PBRs and those with rough superhydrophobic coatings that compromise transparency. These results underscore the critical roles of both antifouling and antireflective properties in optimizing the cultivation of photosynthetic microorganisms, and suggest the potential applications of the proposed coating in the cyanobacterial industry and other bioculture domains.
研究领域:
微生物学
·
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2024
·
IF 5.8
Xinlv Peng,Caixia Pei,Eika W. Qian,Yuguang Du,Jian-Jun Li
6′-Sialyllactose (6′-SL), the most abundant sialylated human milk oligosaccharide, has attracted attention for its potential application in supplementary infant formulas. Herein, we report a facile strategy to construct a cascade bioreactor for the enzymatic synthesis of 6′-SL by co-immobilizing an enzymatic module consisting of CMP–sialic acid synthase and α-2,6-sialyltransferase into hierarchically porous MIL-53 (HP-MIL-53). The as-prepared HP-MIL-53 showed high enzyme immobilization capacity, reaching 226 mg g−1. Furthermore, the co-immobilized enzymes exhibited higher initial catalytic efficiency, and thermal, pH and storage stability than the free ones. Finally, the 6′-SL yield remained >80% after 13 cycles of use. We expect that HP-MIL-53 would have potential industrial applications in the enzymatic modular synthesis of 6′-SL and other glycans.
研究领域:
生物化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Chang Liu,Lin Chen,Liang Zhu
This study was carried out by applying the direct contact membrane distillation (DCMD) into the treatment of effluent from anaerobic membrane bioreactor . The treatment efficiency of DCMD was highly emphasized, which was expected to be improved through the optimization of operating conditions. Three operating conditions, including temperature difference, cross-flow velocity and membrane pore size, were considered. The relative flux (the ratio of actual flux to initial flux) increased from 0.50 to 0.98 as the operating conditions changed and that was enhanced by the increment of temperature difference and cross-flow velocity. Regarding the wastewater treatment efficiency, except for ammonia nitrogen, the interception ratio was greater than 90.0%, which even reached 99.0% for COD Cr , protein and polysaccharide by optimizing operating conditions. In addition, the interception ratio of PO 4 3- -P almost reached 100.0% under any operating condition. Further study about membrane fouling was carried out, and the crystallization fouling was found to be the main fouling type.
研究领域:
环境科学
·
废水处理工程
✓ 已验证
DOI
高影响因子
AICHE JOURNAL
·
2013
·Vol. 59
·期 8
·2743-2752
·
IF 2.493
Pengwei Jin,Ju Chu,Yu Miao,Jun Tan,Siliang Zhang,Weihong Zhu
Microbioreactors with multioptical sensors have become increasingly important because of their small working volumes, high degree of parallelization and the available robotics. A novel hydrophobic luminescent copolymer P(Pt-TPP-TFEMA) along with reference P(Pt-TPP-EMA) containing the pendant group of 5,10,15,20-tetraphenylporphyrin (TPP) moiety as low-cost dissolved oxygen (DO) chemosensor film for high-throughput microbioreactors is designed. Its sensor film exhibits fast response to DO with good stability and fatigue resistance, being capable of applying as a low-cost DO indicator for high-throughput bioprocess measurement. Results show that the quenching response of DO increases with the enhancement in the copolymeric hydrophobicity using the presented hybrid fluorinated ethyl methacrylate. Furthermore, the long emission band at 650 nm of chromophore TPP with large Stoke's shift about 250 nm brings several advantages, such as low scattering, deep penetration, and minimal interferences of absorption and fluorescence from the fermentation system, which shows high-promising application in bioprocess monitoring. © 2013 American Institute of Chemical Engineers AIChE J , 59: 2743–2752, 2013
研究领域:
生物工程
·
化学工程
·
光学传感
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Jiandong Cui,Zhanxin Du,Lixue Zhao,Huihui Li,Zhongjie Wang,Zibei Huo,Yingjie Du,Zhaoming Zhang
Yellow water is a kind of Chinese liquor-making wastewater, and contains rich organic components such as alcohols and organic acids , if not treated, it can cause environmental pollution. Herein, continuous aromatic ester production from Chinese liquor yellow water by a packed-bed column bioreactor with immobilized lipase was achieved. Inspired by interface activation of lipase, lipase from Aspergillus oryzae (AOL) was immobilized on LX-1000HA resin with silicone DC1–2577 modification (SH-LX-1000HA) and oleic acid modification (LX-1000HA-NH 2 -OA), respectively. Benefiting from the hydrophobic properties of the surface, the resultant AOL@SH LX-1000HA and AOL@LX-1000HA-NH 2 -OA were 2.3-times and 1.5-times higher activity than that of free AOL, reached 240 U/g and 156 U/g respectively. Meanwhile, the AOL@SH LX-1000HA and AOL@LX-1000HA-NH 2 -OA exhibited higher thermal stability , storage stability, and recyclability than free lipase. Furthermore, the aromatic ester production from yellow water by an packed-bed column bioreactor increased from 1.293 g/L to 3.976 g/L. This efficient continuous bioprocess presents great industrial potential for high value utilization from Chinese liquor yellow water.
研究领域:
生物工程
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
Bioresource Technology Reports
·
2021
·
IF 0
Mister Adeel,Yubo Xu,Long-Fei Ren,Jiahui Shao,Yiliang He
Extractive membrane bioreactor (EMBR) is a promising technology for phenol saline wastewater treatment . In this work, an electrospun polydimethylsiloxane/polymethyl methacrylate/multi-walled carbon nanotubes (PDMS/PMMA/MWCNTs) membrane was firstly used in EMBR. To address low phenol transmembrane mass transfer issue, effects of wastewater condition (1.3–5.5 L/h circulation rate , 1000–4000 mg/L phenol), biomass addition (without/with pre-acclimated activated sludge) and biofilm formation (50.5–120.7 μm) were investigated. Membrane characterizations confirmed that biofilms on membrane surface gradually developed under increasing phenol concentrations, with simultaneously increased thickness and extracellular polymeric substances (EPS) excretion. Results showed phenol transmembrane mass transfer rates with activated sludge and formed biofilm increased 21.6–31.7% compared to those without activated sludge. Finally, 100% removal of permeated phenol and 99.96% rejection of salt were achieved under wastewater circulation rate of 4.4 L/h with 1000–4000 mg/L phenol and 10 g/L sodium chloride. Relevant findings are of significance in promoting the EMBR application in organic-inorganic composite wastewater treatment.
研究领域:
环境工程
·
膜技术
·
废水处理
·
生物技术
✓ 已验证
DOI
Bioresources and Bioprocessing
·
2022
·Vol. 9
·期 1
·1-11
·
IF 4.983
Liu Yangyang,Sun Qihao,Hao Mengyang,Tan Wen‑Song,Cai Haibo
The application of natural killer (NK) cells as potential antitumor effector cells appears to be valuable for immunotherapies. However, the clinical use of NK cells is limited because the technical difficulties associated with mass production NK cells at sufficiently high numbers represents a great challenge. Ex vivo expansion of NK cells is a key technology for cell therapy. Bioreactor systems can generate homogeneous culture condition and modulate the environmental and biochemical cues. In this study, a novel magnetically controlled bioreactor was developed for supporting NK cells ex vivo expansion. Using synthetic magnetic beads, the stirring device of the magnetically controlled bioreactor generated reduced shearing force. The intermittent magnetic field was applied for magnetic beads movement to homogenize the culture system. NK-92 cells were cultured in the magnetically controlled bioreactor and the expansion and function of expanded cells were investigated on day 8. The results showed that the expansion of NK-92 cells in the bioreactor was 67.71 ± 10.60-fold, which was significantly higher than that of the T25 culture flask (P < 0.05). Moreover, the proportions of CD3−CD56+ cells and cell killing activity of expanded cells in the bioreactor did not reveal any differences compared to T25 flasks. Taken together, this study demonstrated the possibility of magnetically controlled bioreactor as a potent strategy in NK cells production for facilitating cancer immunotherapy.
研究领域:
生物工程
·
免疫学
·
肿瘤学
✓ 已验证
DOI
高影响因子
Frontiers in Environmental Science
·
2023
·
IF 4.6
Zhang Meilin,Li Shaofeng,Sun Jian,Sun Jianping,Wang Lei,Zhao Rui
To reduce the hazard of sulfamethoxazole (SMX) to the water ecology, the removal mechanism of SMX in wastewater was revealed. This study evaluated the PVDF/DA modified membrane in the membrane bioreactor (MBR) toward the removal and degradation of SMX as a recalcitrant micropollutant. A strategy of dopamine for surface modification of polyvinylidene fluoride (PVDF) membrane was proposed, and PVDF/DA membrane was prepared. Subsequently, PVDF/DA was run in the MBR for 60 days, and the reactor could produce good removal ability for SMX after certain acclimation. When the SMX concentration increased from 0.5 to 2 mg/L, liquid chromatography was used for analysis, and the results showed that the average removal rate was above 85%. The removal effect of COD and NH4+-N and the degree of membrane fouling were also evaluated. By measuring the extracellular polymer (EPS) content, the results showed that after 30 days of operation, the protein and polysaccharide contents in the cake layer on the surface of the PVDF membrane were 1.35 times and 1.49 times that of PVDF/DA membrane, respectively, and the PVDF/DA membrane could alleviate membrane fouling, thereby improving the service life of the membrane and reducing its operating cost. Finally, degradation by-products are evaluated to achieve deeper insight into the degradation mechanisms in the MBR process.
研究领域:
环境科学
·
污水处理
·
材料科学
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Huanlong Peng,Wenjia Xie,Da Li,Meirou Wu,Yuguang Zhang,Haixing Xu,Jian Ye,Tingjin Ye,Liang Xu,Yongmei Liang,Wei Liu
It is necessary to study the mechanism of resistance to heavy metals in microbiological processes. In this study, Ochrobactrum MT180101 was used as the microbial source of an membrane bioreactor to investigate its degradation efficiency for electroplating wastewater and the copper-resistant mechanism. Meanwhile, excitation emission matrix-parallel factor, scanning electron microscope , atomic force microscope, fourier transform infrared spectroscopy , X-ray photoelectron spectroscopy and proteome analyses were applied to explain the comprehensive mechanism of the Ochrobactrum MT180101 resisting heavy metal toxicity. The results indicated that the Ochrobactrum MT180101 resisted heavy metal toxicity with the following pathways: i) binding metal cations on cell wall surfaces, ii) generating microbial products such as protein to chelate and stabilize the metal cations, iii) bio-transporting heavy metals from the intramembrane to the outer membrane by means of intracellular transport, and iv) reducing heavy metals through enzyme-mediated biotransformation . The results ensure that Ochrobactrum MT180101 was a copper-resistant bacterium that can be used in the pretreatment or deep treatment of electroplating wastewater.
研究领域:
环境微生物学
·
废水处理工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Yisong Hu,Ying Zang,Yuan Yang,Ao Duan,Xiaochang C. Wang,Huu Hao Ngo,Yu-You Li,Runda Du
Wastewater preconcentration to capture abundant organics is promising for facilitating subsequent anaerobic digestion (AD) to recover bioenergy, however research efforts are still needed to verify the effectiveness of such an emerging strategy as carbon capture plus AD. Therefore, lab-scale anaerobic dynamic membrane bioreactors (AnDMBRs) without and with the addition of zero-valent iron (ZVI) (i.e., AnDMBR1 versus AnDMBR2) were developed for preconcentrated domestic wastewater (PDW) treatment, and the impact of ZVI addition on process performance and associated mechanisms were investigated. The stepwise addition of ZVI from 2 to 4 to 6 g/L improved the treatment performance as COD removal slightly increased and TP removal and methane production were enhanced by 53.3%–62.9% and 22.6%–31.3%, respectively, in consecutive operational phases. However, the average increasing rate of the transmembrane pressure (TMP) in AnDMBR2 (0.18 kPa/d) was obviously higher than that in AnDMBR1 (0.05 kPa/d), indicating an unfavorable impact of dosing ZVI on the dynamic membrane (DM) filtration performance. ZVI that has transformed to iron ions (mainly Fe 2+ ) can behave as a coagulant, electron donor or inorganic foulant, thus enabling the excellent removal of dissolved phosphorous, enhancing the enrichment and activities of specific methanogens and causing the formation of a compact DM layer. Morphological, componential, and microbial community analyses provided new insights into the functional mechanisms of ZVI added to membrane-assisted anaerobic digesters, indicating that ZVI has the potential to improve bioenergy production and resource recovery, while optimizing the ZVI dosage should be considered to alleviate membrane fouling .
研究领域:
环境工程
·
微生物学
✓ 已验证
DOI
高影响因子
International Journal of Environmental Science and Technology
·
2025
·
IF 3.4
Yu R.,Li P.
Bisphenol A (BPA) is a ubiquitous emerging organic contaminant with potential endocrine-disrupting effects. Inefficient removal of BPA from WWTPs are the main routes for it to enter the environment. This study evaluated efficiency of BPA in two full-scale wastewater treatment plants (WWTPs) employing anaerobic/anoxic/oxic-membrane bioreactor with hollow fiber ultrafiltration (AAO-MBR/UF) and multi-level anoxic/oxic activated sludge process (M-AO) with coagulation-flocculation (CF) process. According to the results, both processes achieved effective BPA removal, with AAO-MBR/UF demonstrating higher biodegradation efficiency. The CF process made further removal of BPA after M-AO process. Additionally, we investigated the antagonistic effect of chlorogenic acid (CGA) on BPA cardiotoxicity in zebrafish. CGA significantly mitigated BPA-induced developmental and cardiac toxicity, likely through competitive binding to myosin. These findings highlight the potential removal and the protective role of CGA against BPA cardiotoxicity, offering valuable insights for environmental risk management and potential therapeutic applications.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）