Horticulturae
·
2023
·Vol. 9
·期 9
·1019
·
IF 3.1
Yuan Zhang,Mina Nan,Huali Xue
Radix Astragali(RA) is the root ofAstragalus membranaceusvar. mongholicus (Bunge) P.K. Hsiao. In order to determine targeted and effective methods for treating mold infections in fresh RA, pathogenic fungi were isolated and identified using morphology and molecular biology techniques, and factors affecting the growth of the pathogenic fungi, such as temperature, pH, light, and carbon and nitrogen sources, were analyzed. Changes in the main active ingredients of RA, such as carbohydrates, flavonoids, and saponins, were detected before and after infection with pathogenic fungi. The results showed thatPenicillium polonicum,Trichothecium roseum, andFusarium equisetiwere the main pathogens causing postharvest disease in fresh RA. The optimum growth temperature for the three pathogens was 25 °C;P. polonicumwas able to grow at an alkaline pH of 8–9, whileT. roseumandF. equisetigrew better and had higher levels of sporulation under acidic conditions with pH values of 5–6. Different carbon and nitrogen sources had different effects on mycelium growth and sporulation. Darkness was favorable for the growth and sporulation of the three pathogens. Compared with healthy RA, the total carbohydrate, flavonoid, and saponin contents of the RA samples infected with the three fungi decreased. This study provides a theoretical basis for the scientific and precise control of RA postharvest disease during storage by targeting different pathogens and their growth conditions.Keywords:Radix Astragali;postharvest disease;identification;biological characteristics;active ingredients
研究领域:
药用植物学
·
真菌学
·
植物病理学
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产品关联
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2020
·Vol. 68
·期 2
·381-389
·
IF 1.638
Dawei You,Yue Feng,Can Wang,Chengtao Sun,Yao Wang,Degang Zhao,Guoyin Kai
Tryptophan decarboxylase (TDC, EC 4.1.1.28) catalyzes tryptophan decarboxylation to form tryptamine through the cofactor pyridoxal-5′-phosphate (PLP), a crucial stage in the production of the terpenoid indole alkaloids like camptothecin (CPT). A new gene encoding TDC was identified from the CPT-producing plant Ophiorrhiza pumila by transcriptome analysis, termed OpTDC2. It contained a 1,536 bp open reading frame that encodes a 511 amino acid protein with a molecular mass of 57.01 kDa and an isoelectric point of 6.39. Multiple sequence alignment and phylogenetic tree analysis showed the closest similarity (85%) with the TDC from Mitragyna speciosa . Moreover, the highest expression of OpTDC2 was observed in the O. pumila root. To achieve high-efficiency expression of OpTDC2 in Escherichia coli , we fused the TF tag onto the N-terminal of the OpTDC2. Optimum enzymatic activity was observed at 45 °C, pH 8 and cofactor concentration of 0.1 mM. The catalytic reaction was strongly inhibited by metal ions of Cu 2+ , Zn 2+ , and Fe 2+ . The l -tryptophan was particularly catalyzed compared with d -tryptophan. Besides, the K m and k cat of the OpTDC2 were 1.08 mM and 0.78 Sec −1 , respectively. The results provided information on new functional OpTDC2 that might be used in synthetic biology for the enhanced biosynthesis of CPT in O. pumila .
研究领域:
植物学
·
生物化学
·
分子遗传学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ling Zeng,Qiuting Wu,Lingyue Tang,Xiao Yang,Qi An,Hushan Cui,Qing Jin
Herein, the feasibility of supplementing black wheat flour and buckwheat flour for semi-dried noodles (SBBN) preparation was assessed. Due to the high nutrient and water activity (a w ) of SBBN, it is easy to induce microbial contamination. Therefore, a combination of ozonated water and tea polyphenols treatment was used in the study to investigate its effect on microbiological, quality and physio-chemical characteristics. The results indicate that combined treatment significantly reduced the total plate count (TPC) of SBBN, improved its color, cooking and texture characteristics and antioxidant activity, enhance its umami taste, and have no significant impact on its flavor profiles during storage. Additionally, the free sulfhydryl (SH) content was reduced, indicating that the combined treatment promoted protein disulfide cross-linking. Scanning electron microscope (SEM) further confirmed that the binding ability between starch particles and gluten proteins was enhanced. Thus, the introduction of ozonated water and tea polyphenols into the black wheat-buckwheat dough had a significant effect on the optimization of the macro- and micro-characteristics of noodles.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
高影响因子
PROTEIN SCIENCE
·
2024
·Vol. 33
·期 10
·e5160
·
IF 4.5
Shuai Xu,Zong-Lin Li,Zhi-Min Li,Hong-Lai Liu
L-cysteine is an essential component in pharmaceutical and agricultural industries, and synthetic biology has made strides in developing new metabolic pathways for its production, particularly in archaea with unique O-phosphoserine sulfhydrylases (OPSS) as key enzymes. In this study, we employed database mining to identify a highly catalytic activity OPSS from Acetobacterium sp. (AsOPSS). However, it was observed that the enzymatic activity of AsOPSS suffered significant feedback inhibition from the product L-cysteine, exhibiting an IC 50 value of merely 1.2 mM. A semi-rational design combined with tunnel analysis strategy was conducted to engineer AsOPSS. The best variant, AsOPSS A218R was achieved, totally eliminating product inhibition without sacrificing catalytic efficiency. Molecular docking and molecular dynamic simulations indicated that the binding conformation of AsOPSS A218R with L-cys was altered, leading to a reduced affinity between L-cysteine and the active pocket. Tunnel analysis revealed that the AsOPSS A218R variant reshaped the landscape of the tunnel, resulting in the construction of a new tunnel. Furthermore, random acceleration molecular dynamics simulation and umbrella sampling simulation demonstrated that the novel tunnel improved the suitability for product release and effectively separated the interference between the product release and substrate binding processes. Finally, more than 45 mM of L-cysteine was produced in vitro within 2 h using the AsOPSS A218R variant. Our findings emphasize the potential for relieving feedback inhibition by artificially generating new product release channels, while also laying an enzymatic foundation for efficient L-cysteine production.
研究领域:
生物化学
·
酶学
·
合成生物学
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DOI
PubMed
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-7
·
IF 4.122
Xia Peng-Fei,Li Qian,Tan Lin-Rui,Liu Miao-Miao,Jin Yong-Su,Wang Shu-Guang
Synthetic biology enables infinite possibilities in biotechnology via employing genetic modules. However, not many researches have explored the potentials of synthetic biology in environmental bioprocesses. In this study, we introduced a genetic module harboring the codon-optimized tetracycline degrading gene, tetX.co , into the model host, Escherichia coli , and generated a prototypal whole-cell biodevice for the degradation of a target antibiotic. Our results suggested that E. coli with the tetX.co- module driven by either the P J23119 or P BAD promoters conferred resistance up to 50 μg/mL of tetracycline and degrades over 95% of tetracycline within 24 h. The detoxification ability of tetX was further verified in conditioned media by typical E. coli K-12 and B strains as well as Shewanella oneidensis . Our strategy demonstrated the feasibility of introducing genetic modules into model hosts to enable environmental functions, and this work will inspire more environmental innovations through synthetic biological devices.
研究领域:
环境科学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yantong Liu,Longfei Chen,Le Yu,Jiaomeng Zhu,Fang Wang,Fenghua Jiang,Chengjun Sun,Li Zheng,Yi Yang
The development of engineering microbiology is widely expected to advance carbon neutrality and sustainability in the wastewater industry. Engineering microbiology can deliver desired functions without high treatment costs, complex processes, massive greenhouse gas emissions, and resource waste. Here, we propose a complementary strategy to realize benefited wastewater utilization by configurable spatialized microorganisms-integrated biophotonic systems (CSMBs). The configurable spatialized microorganisms enable enhanced microbial synergy and empower on-demand waste-to-bioenergy conversion through optofluidic-assisted design. The developed CSMBs improve the reaction between light and microorganisms (light harvesting and microorganisms’ photosynthesis) via enhanced natural light photon capture and adjustable optical wavelength. Thanks to these features, in prospective wastewater treatment, the CSMBs breakthrough achieve 89.89% and 91.8% degradation of total nitrogen (TN) and total phosphorus (TP) and 91.98% removal of chemical oxygen demand (COD) within 72 h, respectively. The biomass (the absorbance of 680 nm), lipid content, and chlorophyll-a content of Chlorella in the CSMBs were increased by 56.95%, 62.91%, and 35.41%, respectively, compared to the growth of single Chlorella . The method possesses broad applicability and superior tunability, which can be extended to a wide variety of wastewater treatment and enable more ability in benefited waste utilization.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Min He,Meiyan Fan,Wei Yang,Zhiyun Peng,Guangcheng Wang
A class of new kojic acid hybrids ( 7a-7o ) bearing a 1,2,4-triazine moiety were prepared, and their inhibitory activities and mechanism on tyrosinase were investigated. All derivatives showed good to excellent anti-tyrosinase activity with IC 50 values ranging from 0.34 ± 0.06 μM to 8.44 ± 0.73 μM. In kinetic study, compound 7m was a mixed-type inhibitor with K i and K is of 0.73 and 1.27 μM, respectively. The interaction mechanism toward tyrosinase of compound 7m was further elaborated in combination with molecular docking and various spectral techniques. The results showed that compound 7m could change the secondary structure of tyrosinase to reduce its catalytic activity. Anti-browning assays demonstrated that 7m inhibited the browning of bananas effectively during storage. What’s more, 7m was found to have low cytotoxicity in vitro . In conclusion, compound 7m has the potential to be applied as an anti-browning agent.
研究领域:
药物化学
·
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Jianwei He,Xiaoyan Zhang,Yuanyi Qian,Qiyao Wang,Yunpeng Bai
The environmental accumulation of organophosphates is a serious threat to public health. To detect these xenobiotics , a broad range of sensors has been developed in past decades. However, sensors with high sensitivity and a capability for degrading organophosphates are rare. In this study, “smart” whole-cell biosensors were created by combining synthetic biology approaches with the bacterial quorum sensing (QS) mechanism. The engineered whole-cell biosensor pUC57-QS-DSF-F42 L/E coli DH5α can sense a wide array of phenolic compounds including phenol and p -nitrophenol (p-NP). By optimizing the genetic circuits, the phenol and p-NP detection limits reached 0.1 and 1 μM, respectively. Importantly, by replacing the fluorescence-generated reporter sf GFP with MP-degrading enzyme Po OPH M2 , the whole-cell biosensor pUC57-OPH-QS-DSF-F42 L/E coli DH5α actively degraded 10 and 100 μM methyl parathion (MP), a typical organophosphate pesticide , which was artificially added to the cell culture at different time points in five consecutive degrading experiments, demonstrating its MP sensing and degrading capabilities. The universal design of this new biosensor can be used to create more efficient biosensors to detect and degrade various pollutants in the environment for rapid testing and bioremediation.
研究领域:
环境科学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jiazeng Yang,Zhuoxin Guo,Wenlong Zha,Jiachen Zi
Piceatannol, a stilbene widely found in many types of foods, shows a variety of effects on the cardiovascular system, nervous system, and skin. Its low abundance in nature impedes its development. Microbial cell factories offer a green and sustainable approach to the production of natural products. In this study, we screened two 4-coumarate:coenzyme A ligases (4CLs), nine stilbene synthases (STSs), and five 4-hydroxyphenylacetate-3-hydroxylases (4HPA3Hs) to construct multiple bioconversion routes from p-coumaric acid to piceatannol in a single Saccharomyces cerevisiae strain. Then, the supply of p-coumaric acid and malonyl-CoA was enhanced, and NADPH and FADH2 regenerations were optimized, resulting in establishment of yPi101 which produced 127 mg/L and 442 mg/L piceatannol in a flask and by fed-batch fermentation, respectively. This is the first report of the de novo production of piceatannol in a single S. cerevisiae strain and represents the highest piceatannol yield.
研究领域:
合成生物学
·
微生物工程
·
天然产物合成
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产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
·
2022
·
IF 7.001
Jun Wang,Xuan Li,Peng Li,Lianxu Li,Lingchao Zhao,Shaoguo Ru,Dahai Zhang
Microplastics and polychlorinated biphenyls are ubiquitous in the marine environments. To illuminate their combined biological impacts, juvenile Japanese flounder ( Paralichthys olivaceus ) were exposed to 500 ng/L PCBs alone or 500 ng/L PCBs plus 2, 20, and 200 μg/L 10-μm porous MPs for 21 days. Compared to PCBs alone, co-exposure to PCBs and 20, 200 μg/L MPs reduced fish body length and body weight, and the concurrence of MPs aggravated PCBs-induced thyroid-disrupting effects, including significantly decreased L-thyroxine and L-triiodothyronine levels, more severe damage to the thyroid tissue and gill morphology, and disturbance on the expression of hypothalamus-pituitary-thyroid axis genes. The PCBs concentrations in the seawater were decreased dramatically with the increase of MPs concentrations, confirming that MPs absorbed PCBs from the seawater. Our results demonstrated that MPs enhanced the thyroid disruption of PCBs, suggesting that the risk of MPs and thyroid-disrupting chemicals on marine organisms should be paid more attention.
研究领域:
环境科学
·
海洋生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Wen-Hui Zhang,Yong-Dong Deng,Zhi-Feng Chen,Zhi-Hao Zuo,Yong-Sheng Tian,Jing Xu,Bo Wang,Li-Juan Wang,Hong-Juan Han,Zhen-Jun Li,Yu Wang,Quan-Hong Yao,Jian-Jie Gao,Xiao-Yan Fu,Ri-He Peng
2,4-Dinitrotoluene (2,4-DNT) as a common industrial waste has been massively discharged into the environment with industrial wastewater. Due to its refractory degradation, high toxicity, and bioaccumulation, 2,4-DNT pollution has become increasingly serious. Compared with the currently available physical and chemical methods, in situ bioremediation is considered as an economical and environmentally friendly approach to remove toxic compounds from contaminated environment. In this study, we relocated a complete degradation pathway of 2,4-DNT into Escherichia coli to degrade 2,4-DNT completely. Eight genes from Burkholderia sp . strain were re-synthesized by PCR-based two-step DNA synthesis method and introduced into E. coli . Degradation experiments revealed that the transformant was able to degrade 2,4-DNT completely in 12 h when the 2,4-DNT concentration reached 3 mM. The organic acids in the tricarboxylic acid cycle were detected to prove the degradation of 2,4-DNT through the artificial degradation pathway. The results proved that 2,4-DNT could be completely degraded by the engineered bacteria. In this study, the complete degradation pathway of 2,4-DNT was constructed in E. coli for the first time using synthetic biology techniques. This research provides theoretical and experimental bases for the actual treatment of 2,4-DNT, and lays a technical foundation for the bioremediation of organic pollutants.
研究领域:
环境科学
·
微生物学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pathogens
·
2022
·Vol. 11
·期 6
·673
·
IF 4.531
Jia Chen,Fei Ran,Jinqiao Shi,Tingting Chen,Zhibo Zhao,Zhuzhu Zhang,Linan He,Wenzhi Li,Bingce Wang,Xuetang Chen,Weizhen Wang,Youhua Long
Kiwifruit (Actinidia chinensis) is an important commercial crop in China, and the occurrence of diseases may cause significant economic loss in its production. In the present study, a new pathogen that causes brown leaf spot disease on kiwifruit was reported. The fungus was isolated from an infected sample and identified asFusarium graminearumbased on morphological and molecular evaluation. Koch’s postulates were confirmed when the pathogen was re-isolated from plants with artificially induced symptoms and identified asF. graminearum. Based on the biological characteristics of the pathogen, it was determined that: its optimal growth temperature was 25 °C; optimal pH was 7; most suitable carbon source was soluble starch; most suitable nitrogen source was yeast powder; and best photoperiod was 12 h light/12 h dark. Further investigations were conducted by determining 50% effective concentrations (EC50) of several active ingredients of biological fungicides againstF. graminearum. The results showed that among the studied fungicides, tetramycin and honokiol had the highest antifungal activity against this pathogen. Our findings provide a scientific basis for the prevention and treatment of brown leaf spot disease on kiwifruit.Keywords:kiwifruit;brown leaf spot disease;Fusarium graminearum;biological characteristics;active ingredients of biological fungicides
研究领域:
植物病理学
·
农业科学
·
真菌学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）