TALANTA
·
2026
·
IF 6.7
Dongfang Guo,Xinyu Zhang,Zizhe Li,Meng Dang,Yexuan Mao,Tong Bu,Dan Hai,Lianjun Song,Xianqing Huang,Xiya Zhang
To avoid false-negative or false-positive results in immunoassays caused by non-uniform antibody cross-reactivity during the detection of the sum of albendazole sulfoxide (ABZSO), albendazole sulfone (ABZSO 2 ), and albendazole-2-aminosulfone (ABZNH 2 SO 2 ) (expressed as albendazole residues), the preparation of a group-specific antibody with homogeneous cross-reactivity is crucial for accuracy. Herein, a novel hapten was designed by introducing a urea structure containing active groups from 5-(propylthio)-1H-benzo[d]imidazole-2-amine. Through monoclonal antibody preparation technology, a broad-spectrum monoclonal antibody 9D9 (mAb 9D9) was prepared with IC 50 values of 0.59, 0.98, 0.41 and 1.13 ng/mL for ABZ, ABZSO, ABZSO 2 , and ABZNH 2 SO 2 , respectively. The ability of mAb 9D9 to uniformly recognize ABZ and its three metabolites was attributed to residues Phe-94 of CDRL3, Glu-103 of CDRH3, and Ser-97 of CDRL3, which formed π-alkyl, π-anion, and hydrogen bonds with the sulfur-containing side chain, benzene ring, and secondary amine on the benzimidazole ring, respectively. Finally, a rapid and accurate lateral flow immunoassay was established for the screening of ABZ and its three major metabolites in animal-derived foods.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yueru Ma,Yuqing Yang,Zhiwei Liu,Hongyu Shi,Zixin Jin,Ziwuzhen Wang,Wei Sheng
High-sensitive fluorescence biomimetic sensor for CBZ is developed. • Ni-MOF is combined with specific adsorption capacity of MIP. • Ni-MOF@MIP probe shows high specificity and sensing sensitivity for CBZ. • Adsorption capacity of Ni-MOF@MIP for CBZ has good stability. A novel fluorescence biomimetic sensor with high selectivity and sensitivity for the detection of carbendazim (CBZ) in fruits, vegetables and grains is established. This sensor is assembled from nickel-based metal-organic framework (Ni-MOF) and molecularly imprinted polymer (MIP). Here, Ni-MOF serves as the fluorescent signal source and carrier, and MIP is coated on the surface of Ni-MOF as a biomimetic recognition element for CBZ to prepare Ni-MOF@MIP probe. The fluorescence of Ni-MOF@MIP will be quenched after adding CBZ, and the fluorescence difference can be applied to realize the quantitative detection of CBZ. The sensor shows wide linear range of 0.01 to 5.00 × 10 3 μg/L ( R 2 = 0.996), and the detection limit (LOD) is 8.4 × 10 −3 μg/L (S/N = 3). The recovery of CBZ in spiked fruits, vegetables and grains has been confirmed by high performance liquid chromatography (HPLC). The fluorescent sensor provides a promising strategy for the detection of CBZ residue in fruit, vegetable and grain samples. Download: Download high-res image (163KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Wen Liu,Yanjing Chen,Xurong Yin,Fei Liu,Wenshan Li,Jianna Yu,Guoxing Jing,Wenjie Liu
A on-site and sensitive pesticide residue detection approach has been developed by electrospray ionization-ion mobility spectrometry (ESI-IMS) combined with a surface swab method. Different instrumental parameters, including ESI bias voltage, solution flow rate, and drift tube temperature, were individually optimized for the strong signal response. This analytical method showed a wide linear range, high sensitivity, acceptable repeatability, and a quick response time. Furthermore, the surface swab method was utilized for pretreatment; and hence, several factors were investigated to improve the sample collection technique’s performance. The calibrated recovery results for carbendazim, thiophanate-methyl, thiabendazole, pirimicarb, and thiacloprid in apple, pear, and tomato ranged from 71.4 to 121.2%, with RSDs of 0.4–12.7%, indicating good accuracy and applicability. Therefore, this study confirmed that ESI-IMS coupled with surface swab possesses a broad application prospect in the rapid, simple, and on-site detection of pesticide residues or other detrimental substances in foodstuffs.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-9
·
IF 6.408
Yu Ying,Huang Gengli,Luo Xueli,Lin Wanmei,Han Yong,Huang Jihong,Li Zhonghong
A dual-emission fluorescent (FL) probe was constructed by coordinating Cu 2+ of copper metal–organic frameworks (Cu-MOFs) with − COO − group of carbon dots (CDs) for pesticide thiophanate-methyl (TM) determination. TM was recognized by organic ligands (H 2 BDC and H 2 BDC-NH 2 ) of Cu-MOFs via π stacking. Due to the higher affinity of Cu 2+ to TM than ligands and CDs, TM chelated with Cu 2+ to form TM-Cu complex. Thus coordination of Cu-MOFs was damaged and the ligands were released resulting in the FL intensity increase of Cu-MOFs (F 430 ). And also CDs were released from CDs@Cu-MOFs hybrids and electron transfer from CDs to CuMOFs was inhibited, leading to the FL intensity increase of CDs (F 600 ). The FL intensity ratio (F 430 /F 600 ) showed a good linear relationship with TM concentrations of 0.0307–0.769 μmol L −1 with a limit of detection (LOD) of ~ 3.67 nmol L −1 . The probe was successfully applied to detect TM in spiked food samples with satisfactory recoveries of 93.1–113%. Additionally, visual detection of TM was achieved according to the fluorescence color variation from blue to carmine, indicating promising application of CDs@Cu-MOFs probe. Graphical abstract
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高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Xueli Luo,Gengli Huang,Yuchen Li,Jie Guo,Xiaokai Chen,Youwen Tan,Wenzhi Tang,Zhonghong Li
Ultrasensitive, reliable and prompt pre-warning of pesticide residue is critically significant to ensure environmental and food safety. Herein, a novel nanosensor fabricated by in situ encapsulating N -doped carbon dots into a porphyrin metal–organic framework was developed to ratiometric fluorescence (RF) and smartphone-integrated portable platform colorimetric dual-mode detection of glyphosate (GLP). The sensor responded to GLP by RF in the range of 0.01–6.67 mg/L with low limit of detection of 9.06 µg/L. Besides, a noticeable color evolution from pink purple to green under nature light was observed upon addition of GLP, thus a portable smartphone-integrated colorimetric platform was fabricated for visual colorimetric detecting GLP by incorporating the sensor with a smartphone and a 3D-printed mini-device. Interestingly, the platform can detect GLP merely by virtue of a built-in flashlight of the smartphone and Color Analyser Application. Additionally, desirable recoveries of 94.0–112.5 % and 90.0–113.6 % in spiked real samples were respectively obtained for RF and colorimetric channel. Furthermore, a one-to-one colorimetric logic gate (RGB vs GLP concentrations) offered the possibility of intelligent monitoring of GLP. Summarily, the versatile sensor satisfied laboratory instrumental analysis and was further integrated as a smartphone-assisted platform to furnish a novel tactic for quick GLP monitoring, particularly for resource-constrained settings.
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Journal of Fungi
·
2022
·Vol. 8
·期 5
·547
·
IF 5.724
Gui-Yang Zhu,Ying Chen,Su-Yan Wang,Xin-Chi Shi,Daniela D. Herrera-Balandrano,Victor Polo,Pedro Laborda
Fungal pathogens can invade not only the fruit peel but also the outer part of the fruit mesocarp, limiting the efficacy of fungicides. In this study, the relationships between fungicide structure, diffusion capacity and in vivo efficacy were evaluated for the first time. The diffusion capacity from pear peel to mesocarp of 11 antifungal compounds, includingp-aminobenzoic acid, carbendazim, difenoconazole, dipicolinic acid, flusilazole, gentamicin, kojic acid, prochloraz, quinolinic acid, thiophanate methyl and thiram was screened. The obtained results indicated that size and especially polarity were negatively correlated with the diffusion capacity. Although some antifungal compounds, such as prochloraz and carbendazim, were completely degraded after a few days in peel and mesocarp, other compounds, such asp-aminobenzoic acid and kojic acid, showed high stability. When applying the antifungal compounds at the EC50concentrations, it was observed that the compounds with high diffusion capacity showed higher in vivo antifungal activity againstAlternaria alternatathan compounds with low diffusion capacity. In contrast, there was no relationship between stability and in vivo efficacy. Collectively, the obtained results indicated that the diffusion capacity plays an important role in the efficacy of fungicides for the control of pear fruit diseases.Keywords:fungicides;plant fungal pathogens;pear fruit;antifungal activity;peel diffusion;postharvest diseases
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高影响因子
Surfaces and Interfaces
·
2021
·
IF 4.837
Xiao-Ting Zhen,Ya-Ling Yu,Min-Zhen Shi,Si-Chen Zhu,Tian-Ci Yan,Zi-Xuan Yue,Yu-Xin Gu,Hui Zheng,Jun Cao
The Hawthorn kernel activated carbon (HKAC), acquired from food waste (hawthorn kernel) by a two-step method including carbonization operation and thermally activating potassium hydroxide process, was prepared. Morphological integrity and crystalline state of the HKAC were characterized by scanning electron microscopye and X-ray diffraction. Structural feature of the HKAC was investigated by the Brunauer–Emmett–Teller method, Fourier transform infrared spectroscopy , Raman spectroscopy together with zeta potential . The HKAC contained a specific surface area of 1269.92 m 2 /g and pore volume of 0.67 cm 3 /g. The optimized adsorption conditions were to use HKAC with a carbonization temperature of 550 °C were at a concentration of 0.6 g/L for adsorption in a solution with a temperature of 60 °C and a pH of 6.0. The kinetic studies proved that the pseudo-second-order model fitted experimental data best with the high correlation coefficient values of 0.9968–0.9993. Besides, experimental results revealed that Langmuir isotherm model gave the best fit among the studied isotherm models with a maximum adsorption capacity of 100.00 mg/g, the experimental results revealed that the Langmuir isotherm model was more consistent than the Freundlich isotherm model with a maximum adsorption capacity of 100.00 mg/g, and the adsorption of fungicides onto HKAC was endothermic spontaneous process. Moreover, it was confirmed in tap water and Dendrobium that the removal rate of the six fungicides remained at 86.3% to 96.2% after five cycles of HKAC even in a complex matrix. The aforesaid satisfying experimental results proved that HKAC was a cost-effective and green adsorbent for removing water and plant contaminated by fungicides.
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Journal of Fungi
·
2024
·Vol. 10
·期 9
·622
·
IF 4.2
Yongqing Yang,Yong Wang,Jing Gao,Zhidan Shi,Wenjin Chen,Haiyan Huangfu,Zhengnan Li,Yan Liu
Fusarium crown and root rot (FCRR), caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL), is an economically important disease that affects tomatoes worldwide and has become more prevalent in China in recent years. In 2021 and 2022, tomato plants in greenhouses in Hohhot, Inner Mongolia, were observed showing symptoms of stunting, premature loss of lower leaves, and root rot. Fungal pathogens were isolated from 20 infected tomato plants and identified based on morphological observation and DNA sequencing. Twelve isolates were consistently identified as Fusarium oxysporum f. sp. radicis - lycopersici (FORL) via an analysis of the ITS , TEF- 1α , and pgx4 genes. This is the first report of FORL in Inner Mongolia, China. The isolates were examined for their pathogenicity by inoculating them on tomatoes, eggplants, peppers, and chickpeas. The fungicide sensitivity of the isolates was determined. Effective concentrations for 50% growth inhibition (EC 50 ) were measured using seven fungicides. The EC 50 values of tebuconazole and prochloraz were <1.0 μg·mL −1 , exhibiting the most effective inhibition among the fungicides tested. Additionally, FORL resistance screening of tomato germplasms was performed. One tomato variety was resistant to FORL, and the remaining 43 germplasm lines showed various levels of resistance. The rates of highly susceptible, moderately susceptible, susceptible, and moderately resistant germplasms accounted for 29.55%, 22.73%, 40.91%, and 4.55% of the 44 germplasms tested, respectively.
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jie Zhao,Zi-Yu Li,Ting Cheng,Yan-Xin Chen,Cong Dai,Jia-Hui Zhu,Yu-Shen Liu,Lu-Liang Wang
Benzimidazoles (BZDs) are widely applied and easily accumulated through the food chain and endanger human health. Effective sample pre-treatment technology is the key procedure for trace BZDs analysis. Herein, magnetic covalent organic frameworks (Fe 3 O 4 @TRITER) nanospheres , as magnetic solid-phase extraction (MSPE) adsorbent, were synthesized and well characterized. The efficient extraction of BZDs residues depended on large specific surface of COFs and π-π and hydrophobic interactions between TRITER shell and BZDs. Under the optimal MSPE conditions, the developed MSPE-HPLC based on Fe 3 O 4 @TRITER method for BZDs residues afforded good linearities (0.01–1 μg mL −1 ) with R 2 (≥0.996) and low limit of detections (3 ng mL −1 ). The developed Fe 3 O 4 @TRITER based MSPE-HPLC method was evaluated and successfully applied to determine BZDs residues in orange juice, mixed juice drink and cucumber with satisfactory recoveries (76.9–117 %) and relative standard deviations (0.10–7.90 %).
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Yong Han,Xie He,Weixia Yang,Xueli Luo,Ying Yu,Wenzhi Tang,Tianli Yue,Zhonghong Li
A ratiometric fluorescent sensor was facilely fabricated using innate fluorescence of carbendazim (MBC) and fluorescent UiO-67 to sensitively and selectively detect MBC in food matrixes. The innate fluorescence of MBC provided a signal at 311 nm (F 311 ), and the fluorescent UiO-67 at 408 nm (F 408 ) could recognize MBC through π-π stacking inducing fluorescent quenching relied on photoelectron transfer (PET). The ratio (F 311 /F 408 ) of the fluorescence enhancement of MBC and the quenching of UiO-67 linearly responded to the MBC concentrations of 0–47.6 μmol/L with a low limit of detection (LOD) of 3.0 × 10 −3 μmol/L. The reverse response signals of the sensor enhanced the sensitivity toward MBC and presented remarkable anti-interference capability in complex matrices. The as-prepared sensor was applied to detect MBC residues in apple, cucumber and cabbage, obtaining satisfactory accuracy and precision with the recovery of 90.82–103.45% and RSDs of lower than 3.03%.
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高影响因子
ANALYTICAL LETTERS
·
2020
·
IF 1.467
Xinyue Zhao,Xiaomeng Zhang,Mingwei Qin,Yuzhu Song,Jinyang Zhang,Xueshan Xia,Xiuming Cui,Kai Gao,Qinqin Han
Because of diverse adverse effects of carbendazim (CBZ) upon the human body and environment, a simple method for the determination of this molecule is urgently needed. Therefore, a method based on an aptamer was developed. An aptamer of carbendazim, CZ12, labeled with a fluorescent molecule (FAM), was adsorbed on graphene oxide (GO) by π-π stacking. The fluorescence resonance energy transfer (FRET) between the molecule and GO was used to rapidly quench the fluorescence signal. In the presence of carbendazim, the fluorescent molecule selectively combines with carbendazim, thereby greatly reducing the binding efficiency of the former with GO. Thus, by mixing a FAM-labeled carbendazim aptamer, GO, and food samples, the carbendazim content was determined from the fluorescence measurements. The limit of quantification was 10.0 ng/mL for strawberry, banana, pitaya, and Panax notoginseng samples. The linear range was from 0 to 125 ng/mL with a linear correlation coefficient equal to 0.9918.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Yixia Yang,Behafarid Ghalandari,Liyun Lin,Xiao Sang,Wenqiong Su,Adeleh Divsalar,Xianting Ding
The abuse application of glyphosate can result in a potential hazard for environment and human, however its ultrasensitive detection remains challenging. Herein, a Cu 2+ modulated DNA-templated silver nanoclusters (DNA-AgNCs) sensor was constructed to sensitively determine glyphosate based on the turn-on fluorescence strategy. The fluorescence quenching of DNA-AgNCs occurred with the existence of Cu 2+ . Upon the presence of glyphosate, the functional groups on the surface of glyphosate could chelate with Cu 2+ , following the fluorescence recovery of DNA-AgNCs. Through the stoichiometric methods, we unveil that Cu 2+ -trigged fluorescence quenching mode is a combination of static and dynamic quenching with the static mode being predominant. In DNA-AgNCs/Cu 2+ system, the carboxylate, amine, and phosphonate groups of glyphosate interact with Cu 2+ through chelation, in which the carboxylate oxygen, the phosphonate oxygen atoms, and the monoprotonated secondary amine nitrogen atom and Cu 2+ form chelate rings. This fluorescence sensor showed a desired linearity of glyphosate analysis under the optimum conditions, ranging from 15 to 100 μg/L with a low detection down to 5 μg/L. Moreover, the proposed sensor was successfully utilized to measure glyphosate in real samples, indicating a promising application in pesticide residues detection.
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高影响因子
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