JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yuzhu Zhang,Hao Wu,Zheng Fu,Shilong Zhang,Meiling Zheng,Jiaxia Sun,Zhongxia Lu,Rilei Yu,Wengong Yu,Feng Han
Hyaluronate lyases are widely used in medicine and biochemical engineering and are also applied as a tool enzyme to prepare oligosaccharides with various biological activities. To date, only a few hyaluronate lyases are on sale with poor thermostability. In this study, a PL8 hyaluronate lyase, TcHly8D, was found from Thermasporomyces composti and expressed in Escherichia coli with a maximum yield of 1.77 × 109 U/L (3.14 g/L) in a 5-L bioreactor. The recombinant TcHly8D exhibited a high hyaluronate lyase activity of 5.64 × 105 U/mg and an excellent thermostability with half-lives of 184.9 h at 60 °C. Fifty micrograms of TcHly8D could catalyze 5 g of hyaluronic acid with an oligosaccharide yield of 84.8% in 4 h. The salt bridges, hydrogen bonds, and proline residues, but not disulfide bonds, played important roles in the thermostability of TcHly8D. These findings provide insights into the multifunctional application potential of TcHly8D in agriculture, medicine, and the food industry.
研究领域:
生物化学
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医学
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酶工程
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Siqi Quan,Jingting Zhang,Lanyue Zhang,Na Li,Lijun Zhu,Xiuxia Sun,Jianxi Xiao
Type IV collagen , a principal constituent of basement membranes , consists of six distinct α chains that assemble into both ABC and AAB-type heterotrimers. While collagen-like peptides have been investigated for heterotrimer formation, the construction of ABC-type heterotrimeric collagen mimetic peptides remains a formidable challenge, primarily due to the intricate composition and arrangement of the chains. We have herein for the first time reported the development of a versatile triblock peptide system to mimic ABC-type heterotrimeric collagen stabilized by salt bridges. The triblock peptides A, B, and C incorporate functional natural type IV collagen sequences in the center, along with charged amino acids at their N and C-terminals. By leveraging electrostatic repulsion at these charged termini, the formation of homotrimers is effectively inhibited, while stable ABC-type heterotrimers are generated through the establishment of salt bridges between oppositely charged terminals. Circular dichroism (CD) spectroscopy demonstrated that peptides A, B, and C existed as individual monomers , while they effectively formed stable ABC-type heterotrimers upon being mixed at a molar ratio of 1:1:1. Additionally, fluorescence quenching results indicated that fluorescence-labeled peptides A′, B′, and C′ formed ABC-type heterotrimer, exhibiting comparable thermal stability as determined by CD spectroscopy. Molecular dynamics simulations elucidated the role of salt bridges between arginine and aspartic acid residues at N- and C-terminals in maintaining a unique chain register in the ABC-type heterotrimers. These triblock peptides offer a robust approach for replicating the structural and functional characteristics of type IV collagen, with promising applications in elucidating the biological roles and pathologies associated with heterotrimeric collagen.
研究领域:
生物化学
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分子生物学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Small Methods
·
2019
·Vol. 3
·期 12
·1900474
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IF 0
Yongwen Ren,Chang Yu,Xinyi Tan,Xiaotong Han,Hongling Huang,Huawei Huang,Jieshan Qiu
The electrocatalytic N 2 reduction reaction (ENRR) to ammonia has attracted much attention due to the mild operating conditions, in which the Nafion membrane is generally adopted to separate the ammonia from the anode products. However, it has been mentioned that the Nafion membrane allows ammonia to pass through and traps the ammonia to some extent, but powerful confirmation experiments and effective solutions are still lacking. Herein, it is experimentally confirmed that the use of the Nafion membrane in the ENRR is inappropriate, based on the results that the ammonia can pass through the membrane, be adsorbed on the membrane, and interact with the membrane. This causes a measurement error and accelerates the membrane degradation process, which is unfavorable for ammonia quantification and the long-term stability operation of the ENRR in practical application. Also, for the first time, a more accurate method to evaluate the activity of the ENRR electrocatalyst using a salt bridge to replace the Nafion membrane is presented.
研究领域:
化学工程
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材料科学
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电化学
✓ 已验证
产品关联
DOI
BIORESOURCE TECHNOLOGY
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2025
·
IF 9
Jishan Li,Muyang Li,Chenyu Yong,Zhemin Zhou,Laichuang Han,Zhongmei Liu
UDP-glucose (UDPG), a nucleoside diphosphate sugar, is an important sugar donor for the biosynthesis of various glycoside compounds. Sucrose synthase (SuSy) can catalyze the synthesis of high-value UDPG from the cost-effective sucrose. By redesigning the substrate-binding pocket of SuSy in Denitrovibrio acetiphilus DSM 12809, a variant with 8.1-fold increased activity was generated in this study. Structural analysis revealed that the synergistic effect of the newly introduced hydrogen bonds and salt bridges was crucial for the enhanced activity. The yield of UDPG reached 127.6 g/L/h using this variant for catalysis. Moreover, when cascaded with glycosyltransferase for salidroside production, the yield of salidroside increased 2.7-fold, and a UDP recycling number of 3.2 was achieved. These results provide technical support for the industrial application of SuSy in the biosynthesis of nucleotide sugars and glycosides.
研究领域:
生物化学
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工业微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Qi Xu,Qi-Xing Xie,Tong Xue,Gao Cheng,Jian-Dong Wu,Lan Ning,Xiang-Hui Yan,You-Jun Lu,Zhong-Li Zou,Bei-Ping Wang,Feng-Lan Han
The electrolytic Zn-MnO 2 aqueous battery is an attractive energy storage technology with a high working voltage and energy density for the large-scale application. Here, a three-phase decoupled Zn-MnO 2 electrolytic battery is designed. A salt bridge gel as an intermediate is introduced to separate the catholyte and anolyte in this design. This battery behaves a wide electrochemical stability window and a high discharge voltage of 2.5 V with 95 % capacity retention after 350 cycles. The working mechanism has also been discussed in this study via ex-situ analysis.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
Systems Microbiology and Biomanufacturing
·
2023
·
IF 0
Zhang Landong,Gao Changzheng,Song Wei,Wei Wanqing,Gao Cong,Chen Xiulai,Liu Jia,Liu Liming,Wu Jing
D -p -hydroxyphenylglycine (D-HPG) is an important intermediate in the pharmaceutical industry, and it is commonly synthesized by cascading D-hydantoinase (DHase) and D-carbamoylase (DCase). In this study, the stability of DCase was identified as the main problem that limits its application. Therefore, the complexed structure of Ak DCase (DCase from the Agrobacterium sp . strain KNK712) with the substrate N-carbamoyl-D- p -hydroxyphenylglycine (C p HPG) (with 2.52 Å resolution) and catalytic mechanism were resolved. Based on the catalytic mechanism and electrostatic stabilization, salt bridge engineering was adopted to improve Ak DCase thermostability. The best variant, Ak DCase D30A , increased the T m by 2.91 °C and half-life (t 1/2 ) at 40 and 60 °C by 18.43 h and 23.21 min, respectively. After Ak DCase D30A was assembled with Gs DHase (DHase from Geobacillus stearothermophilus SD-1) in a single Escherichia coli cell, the recombinant strain could produce 29.53 g/L D-HPG within 12 h, with a 97% conversion and a 2.46 g/(L·h) space–time yield (STY). The titer of D-HPG increased by 40.55% compared to the E. coli cell harboring pETduet-1- Ak DCase- Gs DHase. The recombinant strain could be used for two cycles. Our research provides a basis for the industrial production of D-HPG.
研究领域:
生物催化
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蛋白质工程
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工业微生物学
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结构生物学
✓ 已验证
DOI
Acta Geotechnica
·
2025
·
IF 5.7
Yang Guokun,Zheng Shaojun,Liu Tianle,Luo Enhao,Tang Chengxiang,Qu Bo,Lei Gang,Jiang Guosheng
Enhancing the efficiency of microbially induced carbonate precipitation (MICP) for soil stabilization is of significant importance for reducing costs and shortening construction timelines, ultimately improving the applicability of MICP technology in engineering. This study developed a novel biocarrier material (Dia@AP-DE) to enhance the efficiency of MICP in cementing sand by immobilizing microorganisms and diversifying calcium carbonate cementation forms. Dia@AP-DE was chemically modified to graft –NH 2 groups onto its surface. These groups protonate to form positively charged –NH 3 + groups, enabling the tight adsorption of negatively charged bacteria through Coulombic interactions. MICP was applied to reinforce sand columns from group B (free bacteria) and group BDAD (Dia@AP-DE immobilized bacteria), and a series of experiments were conducted to analyze the biocarrier’s effects on cementation efficiency, calcium carbonate morphology, and pore structure. Additionally, flow channel evolution within the sand columns was simulated using AVIZO software. The results showed that the BDAD group formed more diverse calcium carbonate cementation forms, particularly the formation of “calcium carbonate bridges.” The bacteria immobilized on Dia@AP-DE produced additional layers of calcium carbonate, which acted as bridges between sand particles, reducing the distance required for effective cementation. Furthermore, flow simulation results indicated that the “calcium carbonate bridges” optimized the uniformity of flow channels within the sand columns during the early stages of injection. Unconfined compressive strength (UCS) tests showed that the strength of the BDAD group was significantly improved at all injection cycles, with UCS values of 1.02 MPa and 5.88 MPa after 3 and 11 injections, respectively, which were 4.6 and 2.0 times higher than those of B group. This study provides a potential new approach for enhancing the efficiency of biocemented soils.
研究领域:
土木工程
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环境微生物学
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产品关联
DOI
高影响因子
MACROMOLECULES
·
2023
·
IF 5.5
Yuanyuan Liu,Xiyu Wang,Caihui Luo,Jie Cai,Yuting Li,Ying Cao,Yuanli Cai
Salt bridges are predominant in protein construction and stabilization, yet largely unexplored for polymer nanoparticle synthesis. We herein report the use of l-histidine salt bridges to drive monomer preassembly and two-dimensional electrostatic self-assembly in aqueous photo-RAFT polymerization. l-histidine salt bridges drive the monomer clustering nucleation, complex coacervation, and Coulombic stabilization, leading to the 2 nm ultrasmall clusters and coacervate droplets. Homopolymerization leads to a precision two-dimensional electrostatic self-assembly via a droplet-monolayer-multilayer transition, i.e., salt-bridged homo-polymerization-induced self-assembly (PISA). Block copolymerization does not disturb the “salt-bridged homo-PISA” mechanism. Enhanced Coulombic repulsion via seeded polymerization of charged monomers using as-achieved multilayer lamellae (seeds) yields supercharged 5 nm ultrathin monolayer lamellae with high colloidal stability upon dilution, salting, and long-term storage, urgently needed for bioapplications. This work opens up a new avenue to use amino acid salt bridges for PISA synthesis of biologically important, yet hitherto inaccessible, salt-resistant ultrathin polyelectrolyte complex nanomaterials.
研究领域:
聚合物化学
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材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lulu Chen,Jin Qian,Chunmei Xu,Zhuqing Liu,Wenjia Han,Xingxiang Ji
Soy protein adhesives have great potential in replacing traditional petrochemical adhesives to produce eco-friendly and formaldehyde-free plywood. However, weak toughness, poor water resistance and low mold resistance during their usage have severely limited their application in practical production. In this study, we introduced bio-based sodium carboxymethyl cellulose (CMCNa) and lauroyl arginine ethyl ester hydrochloride (LAE) into the soybean meal (SM) system to increase the network interweaving density through the electrostatic interactions and multiple hydrogen bonds between CMCNa, LAE and SM. Both SM and CMCNa can form highly specific guanidinium-carboxylate salt-bridge hydrogen bonds with LAE, leading to the formation of a non-homogeneous phase structure in the system, which can improve the internal energy dissipation and further enhance the toughness and water resistance of adhesive. The results show that the modified adhesive has good toughness, and the wet shear strength is significantly increased by 128 % to 1.07 MPa, and can be stored for >8 days. The tough, waterproofing and mildew resistant bio-adhesive offers a eco-friendly alternative to the traditional chemical adhesive.
研究领域:
材料科学
·
高分子科学
·
胶粘剂技术
·
可持续化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Qiang Chen,Zhi-Pu Li,Xiao-Qiong Pei,Yan Liu,M. Qaiser Fatmi,Zhong-Liu Wu
Chiral epoxides are one of the most versatile building blocks in organic synthesis, but the asymmetric epoxidation of unfunctionalized terminal olefins remains a challenge. Na StyA is a styrene monooxygenase from Nocardia altamirensis that functions as an enantioselective epoxygenase for terminal olefins. To improve its robustness, we investigated over 50 mutants designed via evolutionary- and energy-based approaches, and successfully identified 18 beneficial mutations, which then led to a combinatorial mutant of TM18 with drastically enhanced thermostability and solvent tolerance while retaining excellent activity and enantioselectivity. Its turnover frequency and half-life at 50 °C were increased to 5- and 148-fold of the parental. In the epoxidation of terminal olefins, TM18 achieved high product yields of over 4-fold of the parental, and excellent enantiomeric excess of 99 % to >99 % ee. The analysis of trajectory data from molecular dynamics simulations further validated that the mutations in TM18 increased its local rigidity, as inferred by RMSF and PCA analyses and through novel salt bridges formation. Furthermore, TM18 displayed reduced residue cross-correlation motions. The combined effects of these mutations result in an overall improvement in the structural and dynamic thermostability of TM18.
研究领域:
生物技术
·
酶工程
·
分子生物学
·
化学工程
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）