CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Xueying Guo,Zhiwei Peng,Danlian Huang,Piao Xu,Guangming Zeng,Shuang Zhou,Xiaomin Gong,Min Cheng,Rui Deng,Huan Yi,Hao Luo,Xuelei Yan,Tao Li
Microorganism biotransformation of sulfamethazine (SMT) in aqueous environments is a major concern, especially considering their exposure to coexisting SMT and heavy metals. Phanerochaete chrysosporium ( P. chrysosporium ) is a more concerned Cadmium (Cd) and SMT hyper accumulation specie. This study, referring to metabolic mechanisms and application, was performed to investigate the single and combined effects of Cd-SMT, including toxicity, resistance, as well as the accumulation and biotransformation by P. chrysosporium . The results revealed that Cd-SMT co-contamination caused increasing active oxygen accumulation, and the number of antioxidant enzyme and non-enzymatic antioxidants were higher than that under the stress of their individual pollution. It was found that P. chrysosporium accumulated high levels of Cd with the increment of 6.98–23.96% induced by Cd-SMT co-contamination compared to under the stress of Cd individual pollution. What's more, the addition of Cd reduced the toxicity of SMT to P. chrysosporium . The decrease of malonaldehyde and the increase of protein also proved that P. chrysosporium held enormous potential to fit in the co-contaminated environment, and to remediate the co-contaminated water especially in the long-term treatment. These results undoubtedly contribute to the development of fungi-based technologies and the applications of P. chrysosporium in realistic environment rather than laboratory simulation environment.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Pengjuan Ni,Chuanxia Chen,Yuanyuan Jiang,Yizhong Lu,Wei Chen
Hemin, the most common biological iron form, serves many biological functions. However, studies on hemin detection are scarce. As an unusual natural peroxide, artemisinin is quite stable in the presence of common species. While, it can decompose to generate superoxide radicals in the presence of hemin. And the as-obtained superoxide radicals can effectively oxidize thiamine to form fluorescent thiochrome. Inspired by this, a simple and novel fluorescent assay for hemin detection based on artemisin-thiamine is developed. Based on the linear relationship between the fluorescence intensity and concentration of hemin, the developed artemisinin-thiamine assay can be utilized for sensitive detection of hemin in the concentration range of 2.0–300.0 nM with a detection limit of 0.68 nM. Moreover, this assay shows high selectivity over other cations and biomolecules. Besides, hemin can be detected in serum samples with satisfactory results, which demonstrates its great potential for real sample analysis. The designed assay is free of any fluorescent nanomaterials or probes by just mixing these reagents together, and all the detection processes is performed within 20 min. Therefore, it can offer a simple, highly sensitive and selective sensing platform for fluorescence detection of hemin.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 8
·3982-3988
·
IF 2.936
Yong Li,Zhan-Yao Zhang,Hao-Fan Yang,Guang Shao,Feng Gan
A novel thymine-functional fluorescent sensor was developed for Hg2+ detection with high sensitivity and selectivity. The synthesis of the fluorescent sensor took two steps (1) the synthesis of amine-functionalized carbon dots (CDs-PEI); and (2) the attaching of thymine moieties on to the surface of the CDs-PEI through EDC/NHS coupling chemistry to obtain the thymine-functional fluorescence carbon dots (CDs-Thy). The CDs-Thy were successfully applied to detect Hg2+ by quenching fluorescence with a rapid response through photoinduced electron transfer with the formation of T–Hg2+–T structures. The linear concentration range of Hg2+ is 0–1.0 μmol L−1 and a limit of detection (LOD) as low as 3.5 × 10−8 mol L−1 was obtained. Moreover, the CDs-Thy can resist interference from other metal ions and anions. The CDs-Thy were also used for the Hg2+ detection in water samples and the recoveries were from 90% to 104%. Due to the simplicity and effectiveness, it shows great promise as a potential sensing platform for Hg2+.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Chen Yunhai,Zhu Xuecheng,Liu Huilin,Sun Baoguo
Room temperature phosphorescent carbon dots ( NC CDs@SiO 2 ) were obtained by encapsulating hydrothermally synthesized CDs in a dense Si-O network structure after high-temperature calcination using silica as the matrix. This can avoid the quenching effect of dissolved oxygen in water and has a phosphorescence lifetime of up to 2.41 s. Using the phosphorescence property of NC CDs@SiO 2 , a phosphorescence quenching sensor was developed for the sensitive and selective detection of thiram with the assistance of Cu 2+ . Cu 2+ -thiram complexes led to a rapid phosphorescence quenching of NC CDs@SiO 2 within 30 s through the inner filter effect. The linear range of phosphorescence for thiram was 0.5–100 µM with a detection limit of 0.121 µM. The proposed method was able to detect thiram in real samples and was validated by high-performance liquid chromatography (HPLC) confirming the potential of this phosphorescence sensing method for thiram detection. This work opens up a new avenue for the detection of thiram residues in fruits and vegetables and also provides a new idea for the design of a rapid detection platform using other room temperature phosphorescent materials. Graphical Abstract
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-5
·
IF 5.705
Ni Pengjuan,Chen Chuanxia,Jiang Yuanyuan,Zhao Zhenlu,Lu Yizhong
A fluorometric assay is described for sulfide ions determination. It is based on the finding that the oxidation of the non-fluorescent substrate thiamine (TH) by Cu(II) in basic solution to form fluorescent thiochrome is inhibited by sulfide ions. This results in a decrease in fluorescence intensity which is proportional to the concentration of sulfide ions. Under the optimized conditions, the decrease in fluorescence, best measured at excitation/emission wavelengths of 370/440 nm, decreases linearly in the 0.03 to 2.5 μM sulfide ions concentration range. The detection limit is 20 nM. The method shows excellent selectivity over many potentially interfering ions and has been successfully applied to the determination of sulfide ions in spiked tap water, lake water and the synthetic wastewater samples. The method is time-saving and environmentally friendly, and in our perception shows a great potential in water quality inspection and environmental monitoring.
A fluorescent assay for sulfide ions detection is proposed based on its inhibitory effect on the oxidation of thiamine by Cu(II) ions.
研究领域:
分析化学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 18
·4442-4447
·
IF 3.864
Pengjuan Ni,Dafeng Jiang,Chuanxia Chen,Yuanyuan Jiang,Yizhong Lu,Zhenlu Zhao
In this work, we propose a fluorescence method for the simultaneous detection of glutathione (GSH) and histidine (His) based on the Cu(II)-thiamine (Cu(II)-TH) system. It is well established that non-fluorescent thiamine (TH) can be oxidized by Cu(II) to generate fluorescent thiochrome (TC) under alkaline conditions. The introduction of GSH and His can inhibit the oxidation of TH by Cu(II) due to the strong affinity between Cu(II) and GSH or His. With this strategy, the detection limit for GSH and His is 10.5 nM and 26.4 nM, respectively. The developed method shows several obvious advantages: (1) simplicity in design and operation without the utilization of fluorescent nanomaterials or probes; (2) time-saving detection process since all the detection process is completed within 15 min; (3) cost-effectiveness by using TH as the fluorescent substrate; and (4) higher sensitivity and good selectivity. Therefore, it shows great potential in biosensing fields.
研究领域:
分析化学
·
生物传感
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 6
·1442
·
IF 3.098
Wenxiao Jiao,Xiangxin Li,Handong Zhao,Jiankang Cao,Weibo Jiang
Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE–Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography. The purified protein was identified by MALDI-TOF mass spectrometry, with an estimated molecular weight of 22.1 kDa. BanTLP effectively inhibited in vitro spore germination ofPenicillium expansum, one of the main postharvest pathogens in fruits. This study further investigated the antifungal properties and underlying mechanisms of BanTLP againstP. expansum. Results demonstrated that BanTLP exhibited antifungal activity in a wide pH range (4.0–10.0) at 20–50 °C. Propidium iodide (PI) influx and potassium release confirmed that BanTLP induced membrane disruption of the test pathogen, increasing the membrane permeability and disintegration of the cell. This led to cell death, as evidenced by the assays of thiobarbituric acid-reactive species (TBARS) content, the production of reactive oxygen species (ROS), and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence integrity. Ultrastructural alterations inP. expansumconidia after BanTLP treatment revealed severe damage to the cell wall. These results suggest that BanTLP purified from banana exerts antifungal activity againstP. expansumby inducing plasma membrane disturbance and cell wall disorganization.Keywords:banana;thaumatin-like protein;antifungal activity;Penicillium expansum;mechanisms of action
研究领域:
植物病理学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
Bio-Design and Manufacturing
·
2024
·
IF 8.1
Jing Luyi,Xu Zhaojie,Fan Penghui,Lu Botao,Mo Fan,Hu Ruilin,Xu Wei,Shan Jin,Jia Qianli,Zhu Yuxin,Duan Yiming,Wang Mixia,Wu Yirong,Cai Xinxia
The subthalamic nucleus (STN) is considered the best target for deep brain stimulation treatments of Parkinson’s disease (PD). It is difficult to localize the STN due to its small size and deep location. Multichannel microelectrode arrays (MEAs) can rapidly and precisely locate the STN, which is important for precise stimulation. In this paper, 16-channel MEAs modified with multiwalled carbon nanotube/poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (MWCNT/PEDOT:PSS) nanocomposites were designed and fabricated, and the accurate and rapid identification of the STN in PD rats was performed using detection sites distributed at different brain depths. These results showed that nuclei in 6-hydroxydopamine hydrobromide (6-OHDA)-lesioned brains discharged more intensely than those in unlesioned brains. In addition, the MEA simultaneously acquired neural signals from both the STN and the upper or lower boundary nuclei of the STN. Moreover, higher values of spike firing rate, spike amplitude, local field potential (LFP) power, and beta oscillations were detected in the STN of the 6-OHDA-lesioned brain, and may therefore be biomarkers of STN localization. Compared with the STNs of unlesioned brains, the power spectral density of spikes and LFPs synchronously decreased in the delta band and increased in the beta band of 6-OHDA-lesioned brains. This may be a cause of sleep and motor disorders associated with PD. Overall, this work describes a new cellular-level localization and detection method and provides a tool for future studies of deep brain nuclei. Graphic abstract
研究领域:
神经科学
·
医学工程
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2024
·Vol. 14
·期 12
·911
·
IF 3.8
Shen Yu,Xue-Yong Li,Wen-Jie Si,Ye-Wang Zhang
Formolase, a thiamine pyrophosphate (TPP)-dependent enzyme, catalyzes the carboligation of three one-carbon formaldehyde molecules into one three-carbon dihydroxyacetone molecule. It has many important functions in the biosynthesis of carbon-based compounds and utilization of CO2. However, the enzyme has low activity and stability in the catalytic process, resulting in high cost in the applications. To improve the stability, formolase was immobilized onto magnetic nanoparticles, which were designed to have functional epoxy groups for covalently binding the enzyme. In the immobilization, effects of pH, temperature, and cofactor TPP on the immobilization were investigated and optimized. The results showed that the retention activity of immobilized formolase was highly related to TPP. In the presence of TPP, the specific activity of the immobilized formolase was 6.8 times higher than that without TPP. The optimal immobilization conditions were as follows: a temperature of 20 °C, a pH of 7.0, an immobilization time of 8 h, and an enzyme loading of 20 mg/g. Molecular docking was used to analyze the effect of TPP on the stabilization of the enzyme in the immobilization, which indicated that TTP could stabilize the enzyme structure during the immobilization. The stabilization effect of TPP could be a reference in the immobilization of other enzymes with TPP as the cofactor.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 12
·6869-6876
·
IF 3.361
Shuai Zhang,Huachun Liu,Tianpei Cai,Yanqiang Zhou,Jianmin Li,Xiaoxiao Wang,Shanwen Zhao,Chunmiao Bo,Bolin Gong
Taking thiamphenicol as the research object, a new type of magnetic restricted access molecularly imprinted polymer (RAM-MMIP) with specific recognition was prepared by a one-step swelling method. The polymer microspheres were characterized and analyzed by scanning electron microscopy, X-ray diffraction, elemental analysis, contact angle measurement and vibrating sample magnetometry. When the ratio of template molecule, functional monomer and cross linking agent was 1 : 4 : 8, the adsorption capacity reached the maximum. Under these conditions, RAM-MIP magnetic solid phase extraction (M-SPE) was combined with HPLC to analyze thiamphenicol in milk samples. Satisfactory linear correlation (R2 > 0.9977), good detection limit (LOD: 10.4 μg L−1), high recovery rate (96.5–101.1%), and relative standard deviation (RSD: 2.8–3.8%) were obtained. Therefore, our synthesized material can be used for the analysis of TAP in complex milk samples, and has broad application value.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Hongliang Tan,Qian Li,Zhengchen Zhou,Chanjiao Ma,Yonghai Song,Fugang Xu,Li Wang
Metal-organic frameworks (MOFs) with tunable structures and properties have recently been emerged as very interesting functional materials. However, the catalytic properties of MOFs as enzymatic mimics remain to be further investigated. In this work, we for the first time demonstrated the peroxidase-like activity of copper-based MOFs (HKUST-1) by employing thiamine (TH) as a peroxidase substrate. In the presence of H 2 O 2 , HKUST-1 can catalyze efficiently the conversion of non-fluorescent TH to strong fluorescent thiochrome. The catalytic activity of HKUST-1 is highly dependent on the temperature, pH and H 2 O 2 concentrations. As a peroxidase mimic, HKUST-1 not only has the features of low cost, high stability and easy preparation, but also follows Michaelis–Menten behaviors and shows stronger affinity to TH than horseradish peroxidase (HRP). Based on the peroxidase-like activity of HKUST-1, a simple and sensitive fluorescent method for TH detection has been developed. As low as 1 μM TH can be detected with a linear range from 4 to 700 μM. The detection limit for TH is about 50 fold lower than that of HRP-based fluorescent assay. The proposed method was successfully applied to detect TH in tablets and urine samples and showed a satisfactory result. We believed that the present work could improve the understanding of catalytic behaviors of MOFs as enzymatic mimics and find out a wider application in bioanalysis.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xue Hu,Xidong Liu,Xiaodan Zhang,Haiyan Cao,Yuming Huang
By tuning the photoluminescence of alloy Cu/Ag nanoclusters (NCs) and thiamine (VB 1 ) simultaneously through MnO 2 nanowires, we report a simple and label-free ratiometric fluorescent platform for sensitively and selectively sensing of glutathione (GSH). Ag doping of Cu NCs significantly enhances the quantum yield (QY, about 2-fold enhancement), oxidation resistance to air, storage stability (60 days versus 1 day), photostability and salt stability of the resultant Cu/Ag NCs. Impressively, MnO 2 nanowires quench the fluorescence of Cu/Ag NCs, while they enhance the fluorescence of nonfluorescent VB 1 via oxidation reaction between VB 1 and dissolved oxygen to produce oxVB 1 as a nanozyme catalyst. Upon introduction of GSH as a target, the MnO 2 nanowires are reduced to Mn 2+ and lose their quenching capacity and oxidase-like activity, resulting in FL recovery of Cu/Ag NCs and fluorescent decrease of oxVB 1 . Hence, a ratiometric luminescence signal is obtained, providing a novel ratiometric fluorescent strategy for detecting GSH through target induced regulation of the catalytic activities and quenching ability of MnO 2 nanowires. The sensor exhibited a wide linear concentration range (10 nM to 70 μM) for GSH with a detection limit of 6.5 nM. The ratiometric sensor was successfully used for GSH detection in biological samples.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）