FOOD CHEMISTRY
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2024
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IF 8.5
Jianrui Qi,Kai Wang,Xiaohai Yan,Xinru Dong,Rui Sun,Yahong Yuan,Tianli Yue
The contamination of apple juice by Alicyclobacillus acidoterrestris ( A. acidoterrestris ) can cause significant economic losses. Therefore, developing a rapid and sensitive method for detecting A. acidoterrestris is necessary. To address this issue, this study prepared magnetosome–monoclonal antibodies immunomagnetic microspheres (IMMs) as capture probes to enrich A. acidoterrestris (IMMs-Aa) and monoclonal antibodies-quantum dots (mAb-CdTeQDs) as detection probes for detecting A. acidoterrestris . The obtained IMMs-Aa-mAb-CdTeQDs “sandwich” structure was used to detect A. acidoterrestris based on the fluorescence intensity. The strategy presented a good linear correlation (y = 1048× + 1891, R 2 = 0.995) between the fluorescence intensity and the concentration of A. acidoterrestris (10 1 –10 5 CFU/mL) with a low detection limit of 25.4 CFU/mL within 50 min. The recovery rate of this strategy in spiked apple juice ranged from 88.16 % to 107.03 %. Thus, this study established an efficient and highly sensitive magnetic capture-enrichment-separation-detection method for A. acidoterrestris .
研究领域:
食品科学
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微生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
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2024
·
IF 8.7
Xiaoqing Han,Xingbo Wang,Jiao Yan,Panpan Song,Yanjing Wang,Yaqing Kang,Abdur Rauf,Haiyuan Zhang
The large recruitment of tumor-associated macrophages and low exposure of tumor-associated antigens in tumor microenvironment have severely suppress the efficacy of anti-tumor immunotherapy. Herein, biosynthesized magnetosome (Mag) from bacteria was loaded with photothermal/photodynamic agent/near infrared (NIR) fluorescence dye (IR780) and further modified with lipid-PEG-c(RGDyK) through biomembrane, forming I Mag RGD for fluorescence imaging, magnetic resonance imaging, immunotherapy and photodynamic/photothermal therapy. After intravenous injection into B16F10 tumor-bearing mice, I Mag RGD could efficiently accumulate in tumor tissues based on near infrared (NIR) fluorescence and magnetic resonance dual-modality imaging, and repolarize tumor-associated macrophages (TAMs) from M2 phenotype to M1 phenotype, significantly improving the effect of tumor immunotherapy. Moreover, photothermal and photodynamic effect of IR780 could kill tumor cells and elicit immunogenic cell death to mediate anti-tumor immunity, promoting dendritic cells (DCs) maturation and then activating specific effector T cells to further eliminate tumor cells. This study provides a new approach for reversing the activity of tumor immunosuppressive microenvironment and strengthening the efficiency of tumor photoimmunotherapy.
研究领域:
肿瘤治疗
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生物材料
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免疫学
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分子影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
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IF 10
Xiaoqing Han,Xingbo Wang,Jiao Yan,Panpan Song,Yanjing Wang,Chao Shang,Yunyun Wu,Hua Zhang,Zhenxin Wang,Haiyuan Zhang,Xi Li
Premetastatic niche (PMN) is a prerequisite for tumor metastasis. Destruction of PMN can significantly suppress the tumor metastasis. Bone marrow-derived cells are usually recruited into the premetastatic organs to support PMN formation, which can be orchestrated by tumor-derived secreted factors. Neutrophils can chemotactically migrate towards the inflammatory sites and consume tumor-derived secreted factors, capable of acting as therapeutic agents for a broad-spectrum suppression of PMN formation and metastasis. However, neutrophils in response to inflammatory signals can release neutrophil extracellular traps (NETs), promoting the tumor metastasis. Herein, live neutrophils are converted into dead neutrophils ( C NE) through a quick-frozen process to maintain PMN-targeting and tumor-derived secreted factor-consuming abilities but eliminate NET-releasing shortcomings. Considering macrophages-regulated remodeling of the extracellular matrix in PMN, bacterial magnetosomes (Mag) are further hitchhiked on the surface of C NE to form C NE Mag , which can repolarize macrophages from M2 to M1 phenotype for further disruption of PMN formation. A series of in vitro and in vivo assessments have been applied to confirm the effectiveness of C NE Mag in suppression of PMN formation and metastasis. This study presents a promising strategy for targeted anti-metastatic therapy in clinics.
研究领域:
肿瘤学
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免疫学
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细胞生物学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Yu Mao,Jizi Liu,Jianfei Sun,Yifan Zhao,Yuan An,Lihe Wu,Haikao Feng,Bo Chen,Ruipeng Chen,Kai Zhang,Yan Li,Xiao Huang,Ning Gu
Iron oxide nanoparticles are a kind of important biomedical nanomaterials. Although their industrial-scale production can be realized by the conventional coprecipitation method, the controllability of their size and morphology remains a huge challenge. In this study, a kind of synthetic polypeptide Mms6-28 which mimics the magnetosome protein Mms6 is used for the bioinspired synthesis of Fe 3 O 4 nanoparticles (NPs). Magnetosomes-like Fe 3 O 4 NPs with uniform size, cubooctahedral shape, and smooth crystal surfaces are synthesized via a partial oxidation process. The Mms6-28 polypeptides play an important role by binding with iron ions and forming nucleation templates and are also preferably attached to the [100] and [111] crystal planes to induce the formation of uniform cubooctahedral Fe 3 O 4 NPs. The continuous release and oxidation of Fe 2+ from pre-formed Fe 2+ -rich precursors within the Mms6-28-based template make the reaction much controllable. The study affords new insights into the bioinspired- and bio-synthesis mechanism of magnetosomes.
研究领域:
材料科学
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纳米技术
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生物医学工程
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·
IF 6.6
Na Tang,Yi Zhu,Ziwei Lu,Jiali Deng,Jiajing Guo,Xinyi Ding,Jingyi Wang,Rong Cao,An Chen,Zhongyi Huang,Hongwei Lu,Zhongling Wang
MR-guided focused ultrasound surgery (MRgFUS) is driving a new direction in non-invasive thermal ablation therapy with spatial specificity and real-time temperature monitoring. Although widely used in clinical practice, it remains challenging to completely ablate the tumor margin due to fear of damaging the surrounding tissues, thus leading to low efficacy and a series of complications. Herein, we have developed novel pH-responsive drug-loading magnetosomes (STPSD nanoplatform) for increasing the T2-contrast and improved the ablation efficiency with a clinical MRgFUS system. Specifically, this STPSD nanoplatform is functionalized by pH-responsive peptides (STP-TPE), encapsulating superparamagnetic iron oxide (SPIO) and doxorubicin (DOX), which can cause drug release and SPIO deposition at the tumor site triggered by acidity and MRgFUS. Under MRgFUS treatment, the increased vascular permeability caused by hyperthermia can improve the uptake of SPIO and DOX by tumor cells, so as to enhance ultrasound energy absorption and further enhance the efficacy of chemotherapy to completely ablate tumor margins. Moreover, we demonstrated that a series of MR sequences including T2-weighted imaging (T2WI), contrast-enhanced T1WI imaging (T1WI C+), maximum intensity projection (MIP), volume rendering (VR) and ADC mapping can be further utilized to monitor the MRgFUS ablation effect in rat models. Overall, this smart nanoplatform has the capacity to be a powerful tool to promote the therapeutic MRgFUS effect and minimize the side effects to surrounding tissues.
研究领域:
肿瘤学
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生物医学工程
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纳米技术
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医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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