TALANTA
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2026
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IF 6.7
Peipei Xu,Junpeng Su,Junhua Zhan,Junbo Huang,Junjie Ren,Yu Chen,Lu Chen,Hanhao Lu,Jingbo Chen,Zhenhua Liu,Jing Zhuang,Yudong Lu
Therapeutic drug monitoring (TDM) of mitoxantrone (MIT) is essential for balancing efficacy and toxicity, yet current methods are limited by complex pretreatment, long turnaround times, and matrix interference. Herein, we developed a SERS sensor based on a pectin/AgNPs@CMC/CS/PAAM composite hydrogel for separation-free detection of cationic anticancer drugs in plasma. Fabricated via one-step UV photopolymerization, the hydrogel's dense network provides size-sieving to exclude plasma proteins, while abundant carboxyl groups confer a strong negative surface charge for selective electrostatic enrichment of positively charged drug molecules. This dual mechanism enhances specificity toward cationic analytes in complex matrices. Using MIT as a model, the sensor achieved 82.6%–101.7% recoveries in 10% plasma, a detection limit of 4.7 × 10 −10 M, and excellent reproducibility. Requiring only simple dilution, this platform offers a practical and ultrasensitive tool for TDM of cationic drugs in combination chemotherapy.
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CHEMICAL ENGINEERING JOURNAL
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2026
·
IF 12.5
Bohan Zhang,Jinying Lin,Wenzheng Wu,Zhenglong Chen,Ruiyun You,Yudong Lu,Tao Zhang,Yong Fan
A hydrogel SERS platform requiring no pretreatment has been developed. • The LOD for DOX and MTO were as low as 3.4 × 10 −8 M and 3.6 × 10 −9 M. • Machine learning achieved a 100% identification for DOX and MTO plasma samples. Rapid quantitative detection and classification of trace drugs directly in complex biological fluids are crucial in modern analytical chemistry. This paper proposes a label-free, interference-resistant smart surface-enhanced Raman scattering (SERS) sensing strategy. A machine learning-assisted Au@Ag/C-CNF/PEGDA hydrogel SERS sensor is developed for monitoring and identifying anthracycline anticancer drugs in plasma. Addressing the susceptibility to contamination and oxidation inherent in conventional SERS sensors, this sensor leverages the pore-trapping effect and nanoparticle encapsulation properties of hydrogel. This approach not only eliminates macromolecular interference but also maintains a high signal output of 71.05% for the internal Au@Ag SERS probe after 50 days. Leveraging the electrostatic interactions of carboxylated cellulose (C-CNF), the sensor exhibits excellent signal reproducibility with detection limits as low as 3.4 × 10 −8 M for doxorubicin (DOX) and 3.6 × 10 −9 M for mitoxantrone (MTO). Furthermore, this study employed machine learning models for high-dimensional feature extraction of SERS data, achieving up to 100% classification accuracy for DOX and MTO in spiked plasma samples. This approach holds promise as a robust solution for clinical drug concentration monitoring and species identification, potentially advancing precision treatment strategies in clinical practice. Download: Download high-res image (183KB) Download: Download full-size image
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.2
Chang Liu,Zhilin Hao,Ziming Wang,Kun Cui,Chen Zhang,Jingrong Wang,Lulu Guo,Fuqiang Liu,Qixin Deng,Zi Yang,Xudong Chen,Maojun Yang,Jianping Xie,Sensen Zhang,Jian Mao
Taste perception governs dietary selection, with alkaline sensation playing a pivotal role in food avoidance. In Drosophila, a chloride channel named alkaliphile (Alka) is both necessary and sufficient for aversive taste responses to alkaline food. In this study, we resolved the high-resolution structure of Alka. Alka forms a homopentameric complex, with each subunit comprising four transmembrane helices. Residues P276, T280, and T284 were indicated as key regulators of channel gating. Guided by the structure, we performed molecular docking screening followed by surface plasmon resonance (SPR) validation, identifying three representative ligands that specifically interact with Alka. SPR analysis of Alka mutants confirmed that P276 is essential for ligand recognition, whereas T280 and T284 selectively affect ligand interactions. We also explored the binding dynamics between Alka and ligands. This study elucidates the molecular mechanisms underlying ligand binding and channel gating, providing a theoretical foundation for future studies on alkaline taste perception.
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ACS Omega
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2022
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IF 4.132
Zhi Li,Shuhua Han
A novel type of organic–inorganic hybrid periodic mesoporous materials based on 1,8-dihydroxyanthraquinone (DHAQ-PMOs) was synthesized in a ternary deep eutectic solvent (DES). In the process of the material synthesis, an organosiloxane precursor (DHAQ-Si) and tetraethylorthosilicate (TEOS) were used as the mixed Si source, and cetyltrimethylammonium bromide (CTAB) was used as the structure directing agent. The DES formed by choline chloride (ChCl), urea (U), and ethylene glycol (EG) was used as a green solvent. The water needed for the hydrolysis of DHAQ-Si and TEOS was provided from free water in the sodium hydroxide solution. As characterized by small-angle X-ray scattering, nitrogen adsorption/desorption isotherms, and high-resolution transmission electron microscopy, the obtained mesoporous materials exhibit a two-dimensional hexagonal (P6mm) structure. DHAQ possesses double fluorescence emission peaks at ca. 520 and 580 nm due to the effect of the excited-state intramolecular proton transfer (ESIPT). For DHAQ-PMOs, the silica framework can provide a rigid environment that makes the fluorescence properties more stable and the fluorescence emission peak appears to red-shift obviously. The DHAQ-PMOs have high selectivity and sensitivity in a wide pH range that can be used to detect Cu2+, and the limit of detection (LOD) is as low as 2.39 × 10–9 M. Fluorescence polarization experiments, high-resolution mass spectrometry, and Fourier transform infrared spectroscopy were used to study the coordination interaction between DHAQ and Cu2+. The density functional theory (DFT) was used to further prove the coordination ability and interaction between DHAQ and Cu2+. XPS spectra were also done to prove the coordination of DHAQ and DHAQ-PMO-10 with Cu2+. In addition, confocal fluorescence microscope images exhibit that DHAQ-PMOs can detect Cu2+ in living cells. The results show that DHAQ-PMOs have great application potential as a sensor for the detection of Cu2+.
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JOURNAL OF CHROMATOGRAPHY A
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2020
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IF 4.049
Chunli Lou,Xiongzhen Ye,Ge Chen,Jidong Zhu,Jingwu Kang
Epigenetic inheritance in mammals relies in part on propagation of DNA methylation patterns throughout development. UHRF1 (ubiquitin-like containing PHD and RING finger domains 1) is required for maintenance the methylation pattern. It was reported that UHRF1 is overexpressed in a number of cancer types, and its depletion has been established to inhibit growth and invasion of cancer cells. It has been considered as a new therapeutic target for cancer. In the present work, we described a method for screening inhibitors for blocking the formation of UHRF1–methylated DNA (mDNA) complex by using nonequilibrium capillary electrophoresis of the equilibrium mixture (NECEEM). A recombinant UHRF1 with the SRA domain (residues 408-643), a fluorescently labeled double strand mDNA (12 mer) and a known inhibitor mitoxantrone were employed for proof of concept. The method allows to measure the dissociation constant (K d ) of the UHRF1–mDNA complex as well as the rate kinetic constant for complex formation (k on ) and dissociation (k off ). A small chemical library composed of 60 natural compounds were used to validate the method. Sample pooling strategy was employed to improve the screening throughput. The merit of the method was confirmed by the discovery of two natural products proanthocyanidins and baicalein as the new inhibitors for blocking the formation of UHRF1–mDNA complex. Our work demonstrated that CE represents a straightforward and robust technique for studying UHRF1–mDNA interaction and screening of the inhibitors.
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Nanoscale Research Letters
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2018
·Vol. 13
·期 1
·1-12
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IF 3.125
Tao Xiaojun,Tao Ting,Wen Yi,Yi Jiajin,He Lihua,Huang Zixuan,Nie Yu,Yao Xiaoyan,Wang Yingying,He Chunlian,Yang Xiaoping
Reducing the dosage of chemotherapeutic drugs via enhancing the delivery efficiency using novel nanoparticles has great potential for cancer treatment. Here, we focused on improving mitoxantrone delivery by using cholesterol-substituted pullulan polymers (CHPs) and selected a suitable nano-drug size to inhibit the growth of bladder cancer cells. We synthesized three kinds of CHPs, named CHP-1, CHP-2, CHP-3. Their chemical structures were identified by NMR, and the degree of cholesterol substitution was 6.82%, 5.78%, and 2.74%, respectively. Their diameters were 86.4, 162.30, and 222.28 nm. We tested the release rate of mitoxantrone in phosphate-buffered saline for 48 h: the release rate was 38.73%, 42.35%, and 58.89% for the three CHPs. The hydrophobic substitution degree in the polymer was associated with the self-assembly process of the nanoparticles, which affected their size and therefore drug release rate. The release of the three drug-loaded nanoparticles was significantly accelerated in acid release media. The larger the nanoparticle, the greater the drug release velocity. At 24 h, the IC 50 value was 0.25 M, for the best inhibition of mitoxantrone on bladder cancer cells. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) experiments demonstrated that drug-loaded CHP-3 nanoparticles with the largest size were the most toxic to bladder cancer cells. Immunofluorescence and flow cytometry revealed that drug-loaded CHP-3 nanoparticles with the largest size had the strongest effect on promoting apoptosis of bladder cancer cells. Also, the three drug-loaded nanoparticles could all inhibit the migration of MB49 cells, with large-size CHP-3 nanoparticles having the most powerful inhibition.
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JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Yixuan Zhao,Jiahui Zhao,Shuqin Liu,Dunqing Wang,Jian Liu,Fei Zhang,Xiangshu Chen
Melamine (MA) enhanced Cu-Fenton process was developed for the degradation of anthracyclines. Taking daunorubicin (DNR) degradation as an example, we found that the initial first-order apparent constant of Cu 2+ /MA/H 2 O 2 system with a molar ratio of 1:8 for Cu 2+ :MA was 5.2 times higher than that of conventional Cu 2+ /H 2 O 2 system. The in-situ reductive coordination between Cu 2+ and MA facilitated the generation and stabilization of Cu + species, thereby accelerating the rate-limiting step of Cu 2+ /Cu + conversion and maintaining high levels of Cu + during the degradation process. Moreover, pre-synthesized Cu + -MA complexes ( e.g. , CM-250) further enhanced the efficiency of the Cu-Fenton reaction by increasing both the Cu + proportion and MA chelation. The apparent activation energy for DNR degradation in CM-250 mediated Fenton reaction (15.9 kJ mol −1 ) was lower than that in systems involving Cu 2+ /MA (41.2 kJ mol −1 ) and Cu 2+ (65.6 kJ mol −1 ). Enhanced generation of various reactive oxygen species (·OH,·O 2 - , and 1 O 2 ) was confirmed, with 1 O 2 playing a dominant role, significantly improving both degradation rate and mineralization degree for DNR. MA-enhanced Cu-Fenton process also offers a convenient alternative to effectively remove other anthracyclines and organic micropollutants, holding great promise for advancing advanced oxidation processes as well as practical large-scale degradation applications targeting multiple pollutants.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
·
IF 4.8
Haiying Liu,Yuqi Liu,Xiaoxi Lin,Jianhui Fan,Zhao Huang,Ao Li
Background Lipopolysaccharide (LPS) triggers the activation of nuclear factor kappa B (NF-κB) by interacting with Toll-like receptor 4 (TLR4), leading to the production of various proinflammatory enzymes and cytokines that are crucial in the development of acute lung injury (ALI). Mitoxantrone (MTX) has been demonstrated to mitigate the inflammatory response caused by LPS; however, its precise function in the context of ALI is not fully comprehended. Purpose This study aimed to investigate the inhibitory effects and underlying mechanisms of MTX against LPS-induced ALI. Methods ALI was induced in C57BL/6 mice via a single intratracheal administration of LPS (5 mg/kg), followed by an intraperitoneal injection of MTX to evaluate its therapeutic potential. The effects of MTX on lung injury and the progression of inflammation in ALI mice were assessed using a comprehensive range of techniques, including hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC), myeloperoxidase activity measurement, cell enumeration in bronchoalveolar lavage fluid (BALF), Western blotting, and enzyme-linked immunosorbent assay (ELISA). Additionally, IHC, Western blotting, and co-immunoprecipitation were used to elucidate the specific signaling pathways and molecular mechanisms by which MTX exerted its anti-inflammatory effects in ALI mice. Surface plasmon resonance (SPR) and molecular docking were used to examine the target to which MTX binds directly to reduce inflammation. We also established a lung epithelial cell injury model using LPS-treated A549 cells. The polyubiquitination of IκBα and TRAF6 in LPS-induced A549 cells was detected through Western blotting following immunoprecipitation. Results In mice with LPS-induced ALI, MTX exhibits anti-inflammatory effects by ameliorating histopathological abnormalities caused by LPS, reducing inflammatory cell infiltration, and decreasing the production of proinflammatory enzymes and cytokines. It has been observed that MTX directly binds to the NEDD8 activating enzyme (NAE), thereby inhibiting the transfer of NEDD8 to the substrates UBC12, Cul1, and Cul5. Consequently, the polyubiquitination of IκBα and TRAF6 is disrupted, leading to the suppression of TAK1 activation by TRAF6. This suppression of TAK1 activity hindered the phosphorylation of IKK and MAPK. By stabilizing IκBα through dephosphorylation via IKK inhibition and preventing polyubiquitination, NF-κB activation is reduced. This cascade of events ultimately leads to a reduction in the production of proinflammatory enzymes and cytokines, effectively mitigating the inflammatory response in ALI. In A549 cells, MTX reduces the LPS-induced K48-linked polyubiquitination of IκBα and K63-linked polyubiquitination of TRAF6. This process can be reversed by the overexpression of NEDD8. Additionally, treatment with MG-132, a proteasome inhibitor, can restore the polyubiquitination of IκBα that was inhibited by MTX. Conclusions These findings confirm the essential role of Cul1/5 neddylation in ALI and suggest that MTX could be a promising therapeutic agent for ALI.
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JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2024
·
IF 1.5
Tao Yanru,Zhao Hua,Xiang Yujie,Li Jin,Li Yanting,Hu Jiangling,Wang Hongmei,Jiang Xinhui
The concentration of mitoxantrone in the blood of mice was determined by a high-performance liquid chromatography-ultraviolet method with aloe-emodin as the internal standard. The separation was performed on a Hypersil BDS2 column (4.6 × 250 mm, 5 μm) as the analytical column, the mobile Phase A was acetonitrile, and B was 20-mM potassium dihydrogen phosphate (adding 1% triethylamine and adjusting the pH to 2.8 with phosphoric acid) and 4.6-mM sodium octyl sulfonate. The flow rate was 1.0 mL·min−1, the detection wavelength was 243 nm, the column temperature is 25 ± 5°C and the injection amount was 20 μL. Finally, the linear range of mitoxantrone was 5–200 μg·mL−1, and the correlation coefficient was r = 0.9999. The recovery rate of the method was 91.93–105.5%, and the extraction recovery rate was 91.45–105.5%. The intraday precision and interday precision were <3.29% (limit of detection = 0.3 μg·mL−1). The HPLC method established in this paper was simple, rapid, sensitive and accurate, and can be used to determine the content of mitoxantrone in mouse plasma after tail vein injection.
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Journal of Environmental Sciences
·
2024
·
IF 5.9
Qianyu Xu,Haoyang Fu,Jiyuan Gu,Liyu Lei,Lan Ling
The overuse of antibiotics and antitumor drugs has resulted in more and more extensive pollution of water bodies with organic drugs, causing detrimental ecological effects, which have attracted attention towards effective and sustainable methods for antibiotics and antitumor drug degradation . Here, the hybrid nanomaterial (g-C 3 N 4 @Fe/Pd) was synthesized and used to remove a kind of both an antibiotic and antitumor drug named mitoxantrone (MTX) with 92.0% removal efficiency, and the MTX removal capacity is 450 mg/g. After exposing to the hybrid material the MTX aqueous solution changed color from dark blue to lighter progressively, and LC-UV results of residual solutions show that a new peak at 3.0 min (MTX: 13.2 min) after removal by g-C 3 N 4 @Fe/Pd appears, with the simultaneous detection of intermediate products indicating that g-C 3 N 4 @Fe/Pd indeed degrades MTX. Detailed mass spectrometric analysis suggests that the nuclear mass ratio decreased from 445.2 (M + 1H) to 126.0 (M + 1H), 169.1 (M + 1H), 239.2 (M + 1H), 267.3 (M + 1H), 285.2 (M + 1H), 371.4 (M + 1H) and 415.2 (M + 1H), and the maximum proportion (5.63%) substance of all degradation products (126.0 (M + 1H)) is 40-100 times less toxic than MTX. A mechanism for the removal and degradation of mitoxantrone was proposed. Besides, actual water experiments confirmed that the maximum removal capacity of MTX by g-C 3 N 4 @Fe/Pd is up to 492.4 mg/g (0.02 g/L, 10 ppm).
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ACS Applied Nano Materials
·
2025
·
IF 5.5
Hongjie Zhang,Rui Geng,Benyan Zheng,Jing Wang,Yuan Hu,Xiaopeng Ma
Mitoxantrone (MTO)-based combination therapies hold great promise in enhancing therapeutic outcomes for aggressive cancers, such as triple-negative breast cancer (TNBC). In this study, we designed a core–shell nanoparticle system (MTO@IR780@BSA-GA-Fe@PLGA, PMIBs) that integrates MTO chemotherapy, sonodynamic therapy (SDT) via IR780, and Fenton reaction-enhanced oxidative stress. The system demonstrated pH-responsive release of therapeutic agents, mitochondrial targeting, and synergistic anticancer effects when combined with ultrasound (US) irradiation. PMIBs significantly enhanced reactive oxygen species (ROS) production, increased mitochondrial damage, and improved apoptosis rates, resulting in a tumor growth inhibition rate far superior to that of conventional treatments. These findings highlight the potential of this US-enhanced nanoplatform in addressing challenges associated with TNBC treatment, offering a highly effective and minimally invasive therapeutic strategy.
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