JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2021
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IF 3.205
Xiao-Pan Liu,Wen-Qian Sun,Tong-Xin Liu,Bing-Bing Liu,Chang-Po Chen
Due to the strong background interferences in the low-mass region and poor reproducibility of conventional organic matrices, it is of great importance to develop a novel matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to qualitatively and quantitatively analyze small molecules. In this work, water-soluble fullerenol C 60 (OH) 24–26 was selected as a MALDI matrix for the analysis of low-molecular-weight compounds in consideration of optical absorption property, water solubility and stability. Compared with the traditional matrices, fullerenol demonstrated lower background interference and stronger peak intensity. In addition, the hydrophilic fullerenol could avoid the heterogeneous crystallization in sample preparation, increase the reproducibility and sensitivity of MALDI-MS, and ameliorate quantitative analysis of small molecules. With saccharin as model analyte, quantitative analysis was carried out using fullerenol as matrix. The results demonstrated satisfying reproducibility and good tolerance to salt. The limit-of-detection of the quantitative analysis was as low as 4 pmol, and the linear range is 1–100 μg mL −1 with R 2 greater than 0.99. The analytical results also showed excellent precision and accuracy, low matrix effect and good recovery rate. Fullerenol as a potential matrix was further validated in the quantification of saccharin sodium in different real food samples, such as nuts and drinks. This work not only confirms the potential of fullerenol for the quantitative analysis in food field, but also provides a new technique for rapid analysis of small molecules.
研究领域:
分析化学
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食品科学
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质谱技术
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
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2020
·Vol. 13
·期 8
·1673-1680
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IF 2.667
Cheng Zhu,Ran Qin,Liu Jie,Deng Xing,Qiu Huidong,Jia Zhengfu,Su Xiaodong
Preservatives and sweeteners are always added in the food production process, which can extend the life and flavor of food. Benzoic acid and saccharin sodium are commonly used as preservatives and sweeteners respectively. Phenyllactic acid as a natural preservative, mainly produced by phenylalanine fermentation, may exist in fermented food. The objective of the current study is to develop a reverse-phase HPLC-UV method for simultaneous determination of benzoic acid, sorbic acid, phenyllactic, phenylalanine, and saccharin sodium in vinegar. They were separated on Sapphiresil TM (C 18 ) column (250 × 4.6 mm, 5 μm) with a mobile phase consisting of methyl alcohol:ammonium acetate buffer (pH 6.44; 0.02 M) (20:80, v/v) ran in isocratic mode at a flow rate of 1 mL/min. Complete baseline separation of five analytes was achieved in < 12 min, the linear range was 0.25–50 μg/mL with a correlation coefficient R 2 value > 0.9975, the method was validated with recoveries in the 96.93 to 118.42% range, and RSD was 0.39–6.21%. The detection limits of phenylalanine, benzoic acid, saccharin sodium, sorbic acid, and phenyllactic acid were 0.25, 0.01, 0.01, 0.025, and 0.05 mg/L. This method could be used for determining of benzoic acid, sorbic acid, phenyllactic acid, phenylalanine, and saccharin sodium in vinegar.
Graphical abstract
研究领域:
食品科学
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分析化学
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食品安全
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DOI
高影响因子
THERIOGENOLOGY
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2024
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IF 2.4
Wenjiao Liu,Han wang,Qi Mu,Ting Gong
Taste receptor type 1 subunit 3 (T1R3) is a G protein-coupled receptor encoded by the TAS1R3 gene that can be specifically activated by certain sweeteners or umami agents for sweet/umami recognition. T1R3 is a potential target for regulating male reproduction. However, studies on the impact of non-nutritive sweeteners on reproduction are limited. In the present study, we evaluated the impact of the non-nutritive sweeteners (saccharin sodium, sucralose and acesulfame-K) on testosterone synthesis in testicular Leydig cells of Xiang pigs by comparing the relative abundance of mRNA transcripts and protein expression of T1R3, steroidogenic related factors, and intracellular cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), as well as testosterone levels using Western blotting, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). To clarify the specific mechanism, a dual luciferase assay was used to uncover the relationship between the transcription factors and steroidogenic enzyme. The acute intratesticular injection of a typical non-nutritive sweeteners was conducted to verify this impact in mouse. The results showed that saccharin sodium not only enhanced T1R3 expression in Leydig cells of Xiang pigs, but also caused significant increases in testosterone, cAMP, PKA, phosphorylation of specificity protein 1 (p-SP1), total protein of specificity protein 1 (SP1), steroidogenic acute regulatory protein (StAR), and 3β-hydroxysteroid dehydrogenase type 1 (3β-HSD1) ( P <0.05). Similarly, treatment of Leydig cells with sucralose and acesulfame-K also increased testosterone level, protein expression of T1R3, 17-α-hydroxylase/17, 20-lyase (CYP17A1), and 3β-HSD1 ( P <0.05). Treatment with SQ22536 (an adenylate cyclas inhibitor) or H89 (a PKA inhibitor) significantly reduced saccharin sodium-induced protein levels of p-SP1, StAR, CYP17A1, and 3β-HSD1 ( P <0.05). In addition, a dual luciferase assay further demonstrated that SP1 significantly increased the promoter activity of CYP17A1 ( P <0.05). When mouse testes were injected with saccharin sodium, T1R3, p-SP1, CYP17A1, and 3β-HSD1 were upregulated, leading to a significant testicular increase in testosterone and cAMP levels ( P <0.05). These results suggest a mechanism by which the taste receptor T1R3 regulates testosterone production, and this mechanism may be linked to the cAMP-PKA pathway. Understanding the interrelationship between T1R3 and the cAMP-PKA-SP1 pathway contributes to clarify the regulatory mechanisms of male reproduction.
研究领域:
生殖生物学
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内分泌学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
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2025
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IF 3.9
Zhigao Ma,Ziwen Wei,Bingyan Jiang,Lu Zhang
The electrodeposition of Ni–P–PTFE nanocomposites offers an effective approach to reduce mold–polymer interface adhesion and improve the replication of polymer micro features in injection molding. However, it is still challenging to fabricate Ni–P–PTFE nanocomposites with low internal stress and nanoscale surface roughness in electrolytes containing high PTFE concentrations. In this study, cetyltrimethylammonium bromide was introduced into the electrolytes to achieve a good dispersion of PTFE nanoparticles. Ni–P–PTFE nanocomposites with various compositions were successfully electrodeposited, and their tribological properties were analyzed. The electroformed Ni–P–PTFE nanocomposites with high PTFE and phosphorus contents showed a low surface roughness and surface energy compared to nickel electrodeposits. Ni–P–PTFE nanocomposites also exhibited a low coefficient of friction (COF) and wear loss when electroformed from an electrolyte containing 32 g/L phosphorus acid and 5 g/L PTFE nanoparticle. The COF of Ni–P–PTFE against stainless steel and poly(methyl methacrylate) was 0.2 and 0.12, respectively, representing a decrease of 80% and 85% compared to nickel electrodeposits. These enhancements in self-lubricating performances were primarily attributed to the low surface roughness and a shift in wear modes from adhesive wear to predominantly mild abrasive wear.
研究领域:
材料科学
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表面工程
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摩擦学
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电化学
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制造工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2026
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IF 9.8
Ruidan Li,Li Tian,Yujia Song,Yanjia Guo,Guangping Ma,Pengfei Han,Hanyue Jiang,Wenzhuo Wang,Tengying Ma
In this paper, a dual-mode electrochemiluminescence-electrochemical (ECL-EC) sensor was constructed using Ru(bpy) 3 2+ @Ti 3 CN and Zn 4 Sn 3 O 5 via multi-step amplification strategy for the detection of sodium saccharin (SS). The maximum ECL/EC initial signals were obtained by the synergistic action of Ru(bpy) 3 2+ @Ti 3 CN and Zn 4 Sn 3 O 5 in the tri-n-propylamine (TPrA) solution. Given that SS reacted with Ru(bpy) 3 2+ , the ECL/EC signal values both decreased. The relevant mechanism was investigated. Under the best optimization conditions, the ECL detection exhibited a linear range of 5.0 × 10 −15 to 5.0 × 10 −10 mol·L −1 with a limit of detection (LOD) of 1.67 × 10 −15 mol·L −1 (S/N = 3), while EC detection showed a linear range of 5.0 × 10 −9 to 5.0 × 10 −4 mol·L −1 with the LOD of 1.67 × 10 −9 mol·L −1 (S/N = 3). Satisfactory results were obtained in a variety of actual samples.
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产品关联
DOI
PubMed
高影响因子
ChemSusChem
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2025
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IF 6.6
Muhammad Wasim,Muhammad Tayyab,Ali Arbab,Abdul Zeeshan Khan,Zulakha Zafar,Muhammad Arshad,Rida Javed,Maira Liaqat,Xian-Zhu Fu,Jing-Li Luo
Developing cost-effective, eco-friendly electrocatalysts with strong electrochemical performance is essential for advancing in water electrolysis. Noble metal-based catalysts offer excellent activity, but due to less availability and more expenses leads to an urgent demand for efficient, affordable, and earth-abundant alternatives. In this study, a bimetallic foam architecture (CuF@Ni) was fabricated by electrodeposition of Ni on CuF. This structure acts as both a conductive support and an active electrocatalyst. The synthesized CuF@Ni demonstrated superior HER activity and overpotential of 0.18 V at 50 mA/cm², while for MOR overpotential was observed 0.14 V at 50 mA/cm². CuF@Ni electrocatalytic electrode achieved a Faradaic efficiency ~100 %, with a formate (HCOO-) concentration of 7.9 mM during 150 C charge transfer. In a co-generation system integrating HER and MOR the onset potential was observed as lower 0.4 V and CuF@Ni required only 0.92 V to sustain the current density of 50 mA/cm², demonstrating significant energy savings. In addition, device consumes less power than theoretical water electrolysis under higher current density. Long-term stability tests confirmed minimal degradation under the harsh conditions. This study highlights CuF@Ni as a promising dual-functional electrocatalyst for efficient energy conversion for future renewable energy applications.
研究领域:
能量转换
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电化学
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材料科学
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催化
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可再生能源
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纳米技术
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化学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Research and Technology-JMR&T
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2025
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IF 6.6
Guangmao Yin,Junqing Han,Yuxi Zhu,Xinxin Song,Kexing Song,Yuying Wu
Extremely thin copper foils are critical for high-speed and high-frequency electronic devices, as they significantly reduce signal transmission losses and minimize heating effects. These foils are typically fabricated by depositing onto a carrier foil coated with a stripper layer, where the design of the stripper layer is pivotal. Although benzotriazole coating is a technically mature organic stripping layer, its potential carcinogenicity and environmental persistence may pose long-term risks to human health and the ecological environment, instead of this, a novel composite stripper layer was developed in this work, which comprises a nickel metallic interlayer and an adenine-based organic layer for extremely thin copper foil fabrication. The peel strength of the resulting copper foils (thicknesses: 3.2 μm, 6.5 μm, and 9.7 μm) was evaluated under a constant current density of 20 A/dm 2 , yielding values of 0.012 N/mm, 0.010 N/mm, and 0.009 N/mm, respectively. Variations in current density (8.1 A/dm 2 , 15 A/dm 2 , and 20 A/dm 2 ) further revealed peel strengths of 0.004 N/mm, 0.010 N/mm, and 0.012 N/mm, indicating that the peeling process is relatively easy. Combined theoretical and experimental analyses elucidated the formation mechanism of the composite stripper layer, demonstrating that the adenine-based organic layer adsorbs onto the nickel surface via N–Ni bonding. This mechanism further offers insights into the design of composite peel-off layers, enabling the preparation of extremely thin copper foils. Such a process holds significant potential for the extensible fabrication of conductive patterns in radio frequency identification (RFID) circuits, flexible printed circuits (FPCs), and other advanced electronics applications.
研究领域:
材料科学
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电子材料
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表面工程
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电化学
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柔性电子学
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产品关联
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
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2025
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IF 5.4
Xiaofang Tian,Meijie Xie,Xinyu Luo,Boyan Zheng,Yongxia Ao,Jiaxin Liu,Mei Wang,Qianchun Zhang,Yun Wu
The detection of food pigments is essential for ensuring food quality and safety. This study presents the efficient preparation of fluorine-doped carbon quantum dots (F-CQDs) through a simple solvothermal reaction using Zanthoxylum and NH 4 F as precursors. The resulting F-CQDs displayed optimal fluorescence characteristics, along with a quantum yield of 4.81 %. Notably, the as-prepared F-CQDs exhibited excellent photostability and were effectively quenched by amaranth, demonstrating their potential as reliable probes for amaranth detection. The probe showed a strong linear response ( R 2 = 0.9992) to amaranth concentrations of 0.15–75 μM, yielding a limit of detection as low as 50 nM. Validation tests across various food matrices, including bayberry, a sugar-sweetened beverage, and candies, yielded good recovery rates of 89.0 %–119 % and a relative standard deviation of less than 4.9 % ( n = 5), indicating high reliability. These findings suggest that F-CQDs are viable candidates for detecting amaranth in foods, which can help enhance food safety and quality control.
研究领域:
食品科学
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分析化学
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纳米技术
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荧光光谱学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Qianchun Zhang,Jiaxin Liu,Yongxia Ao,Fengling Tian,Xiaofang Tian,Xinyu Luo,Meijie Xie
Red-emissive carbon dots (CDs) play a crucial role in various food detection applications due to their strong fluorescence in the red luminescence region. Typically, nitrogen-doped CDs are synthesized from diverse precursors and predominantly emit blue fluorescence, which limits their advancement. In this study, we developed an efficient strategy to synthesize novel red-emissive nitrogen-doped carbon dots (R-NCDs) from L-malic acid and urea using a simple solvothermal approach. The synthesized R-NCDs exhibited optimal excitation and emission wavelengths of 540 nm and 630 nm, respectively, and achieved a quantum yield of 14.3 %. Additionally, the fluorescence of the R-NCDs was effectively quenched by the food coloring agent amaranth, allowing for a linear range of 0.050–75 µM and a detection limit of 15 nM. Furthermore, we established a new and efficient fluorescence detection platform capable of detecting trace amounts of amaranth in soda, fruit gummies, hard candies, vodka, preserved fruits, juices, and cocktails, yielding recovery rates ranging from 88.0 % to 119 % with a relative standard deviation of 1.1–3.9 %. Notably, this study is unique as it presents the first synthesis of R-NCDs based on L-malic acid and urea for the detection of amaranth content in various food samples, emphasizing its novelty and significance.
研究领域:
食品科学
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材料化学
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分析化学
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产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
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2024
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IF 5.3
Zhigao Ma,Bingyan Jiang,Dietmar Drummer,Lu Zhang
An electrolyte solution incorporating sodium saccharin and an alkynyl compound was provided to electroform Ni-P-PTFE mold inserts with both low internal stress and good self-lubricating properties. The results showed that with 5 g·L −1 sodium saccharin and 1 mL·L −1 alkynyl compound, the internal stress reached a minimum of −114 MPa, an 82 % reduction from the −646 MPa observed without additives. The presence of sodium saccharin and alkynyl compound in the electrolyte solution reduced the hydrogen evolution reaction current from 15.2 to 12.9 mA at the operating cathode potential of −1 V and decreased the RTC (111) from 100 % to 90 %. The reduction of internal stress in the electrodeposited Ni-P-PTFE composites was attributed to the decreased hydrogenation strain, diminished Ni (111) texture intensity, and the partial incorporation of alkynyl compound reaction products into the deposits, which weakened the connections between crystallites. Finally, 5 g·L −1 sodium saccharin and 1 mL·L −1 alkynyl compound was applied to electroform Ni-P-PTFE mold insert with micro features. Only slightly pile-up defects at the corner of grooves were observed on the polymer chips demolded from Ni-P-PTFE mold insert, demonstrating its good self-lubricating property.
研究领域:
材料科学
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电化学工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Jing Xiao,Dali Zhuo,Jiayuan Tang,Jihong Chen,Chao Tan,Shu Zhang,Shichao Li,Zhirong Zou
The analysis of cyclamate in food samples holds a significant implication for human health, necessitating the development of a selective, sensitive and user-friendly analytical method for analysis of cyclamate. Herein, a pressure meter-based analytical kit was established and applied to selective and sensitive detection of cyclamate. Cyclamate undergoes a selective reaction with NaNO 2 within a hermetically sealed reaction vessel. The reaction produces cyclohexene and N 2 , leading to a discernible pressure change that can be accurately measured using a portable gas pressure meter. Within the concentration range of 20–1000 mg/L, gas pressure values exhibit a proportional relationship with cyclamate concentrations, with a limit of detection of 5.6 mg/L. This analytical kit was further applied to assess cyclamate levels in various beverages and a standard quality control sample (BWQ8791-2016), with recoveries of 93–108 %. It is a simple, easy used, selective and sensitive analytical system for analysis of cyclamate in beverages.
研究领域:
食品安全
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分析化学
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产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
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2024
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IF 5.3
Zhigao Ma,Bingyan Jiang,Dietmar Drummer,Lu Zhang
In micro-injection molding, polymer/mold interface adhesion may cause severe deformation or damage of micro features on the molded products. To reduce the interface adhesion and improve the molding quality of polymer microfluidic chip, Ni-P-PTFE (polytetrafluoroethylene) ternary composite was adopted to electroform mold insert with self-lubricating properties. The influence of phosphorous acid concentration on the composition and performance of Ni-P-PTFE composites was systematically investigated. Our results demonstrated that the content of P and PTFE particles in the composites raised with an increase in phosphorous acid concentration. Elevated phosphorous acid concentration adversely affected the current efficiency and slowed down the deposition rate, allowing sufficient contact time for PTFE to transfer and adsorb onto the electrode surface. The hardness of the deposited nickel increased from 310 HV to 510 HV in the Ni-P-PTFE composites. Furthermore, the Ni-P-PTFE composites demonstrated excellent self-lubricating properties, showcasing friction coefficients of 0.15 and 0.25 during the initial and steady stages, respectively, representing a notable reduction of 66.7 % and 64.3 % compared to pure nickel. This study provides a novel approach to fabricate molds with self-lubricating properties, which contributes to the high-precision injection molding of polymer-based micro devices.
研究领域:
材料科学
·
制造工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）