Polymers
·
2021
·Vol. 13
·期 3
·442
·
IF 4.329
Bilal Muhammad Khan,Li-Xin Zheng,Wajid Khan,Aftab Ali Shah,Yang Liu,Kit-Leong Cheong
Marine rhodophyte polysaccharides have a wide range of described biological properties with nontoxic characteristics, and show great potential in prebiotics and the functional foods industries. However, there is a virtual lack ofGracilaria blodgettiipolysaccharides (GBP) profiling and their bioactivities. This study was designed while keeping in view the lack of physical and chemical characterization of GBP. This polysaccharide was also not previously tested for any bioactivities. A linear random coil conformation was observed for GBP, which was found to be a polysaccharide. A significant sulfate (w/w, 9.16%) and 3,6-anhydrogalactose (AHG,w/w, 17.97%) content was found in GBP. The significant difference in its setting (27.33 °C) and melting (64.33 °C) points makes it resistant to increasing heat. This, in turn, points to its utility in industrial scale processing and in enhancing the shelf-life of products under high temperatures. A radical scavenging activity of 19.80%, 25.42% and 8.80% was noted for GBP (3 mg/mL) in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (ABTS) and hydroxyl radical (HO) scavenging assays, respectively. Therefore, the findings suggest thatGracilaria blodgettiipolysaccharides display a good antioxidant potential and may have potential applications in the functional food industry.Keywords:red algal;Gracilaria blodgettii;polysaccharide;antioxidant
研究领域:
海洋生物学
·
食品科学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Gang Yu,Qiaozhi Zhang,Yanbo Wang,Qing Yang,Hejun Yu,Huan Li,Jian Chen,Linglin Fu
Marine red algae are rich sources of sulfated polysaccharides as bioactive ingredients and hydrocolloid molecules. In this study, sulfated polysaccharides (YGAP, EGAP, and NGAP) were extracted from Gelidium amansii s that were harvested from three different sea areas of China. Characterization of these polysaccharides shows that they contain 3.7–4.0% sulfate and are mainly composed of galactose , glucose, fucose, arabinose, and mannose . The FT-IR and 13 C NMR analysis indicate that the sulfated polysaccharides are agar-type galactans containing 3,6 anhydro-α- l -galactopyranose and β- d -galactopyranose residues. By using various in vitro models, all sulfated polysaccharides, particularly EGAP, exhibit the ability to scavenge O 2 •– and OH· radicals and reducing power. Additionally, in a glucose-glycated BSA system, the polysaccharides show an inhibitory effect on protein glycation and formation of specific advanced glycation end-product. Their anti-glycative property is attributed to their anti-oxidant activity and trapping capacity of methylglyoxal. Given that these polysaccharides possess agar-like structures, they were used to prepare bioactive films and expectedly, transparent and homogeneous films were obtained. The films display high water barrier properties with retained antioxidant and anti-glycation activities as above. These results expand the current knowledge of G. amansii s polysaccharides as nutraceuticals and hydrocolloids, which would benefit their applications in the food industry.
研究领域:
食品科学
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海洋生物学
·
营养学
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2021
·
IF 4.401
Minxi Wan,Haoyu Zhao,Jiacai Guo,Lulu Yan,Daojing Zhang,Wenmin Bai,Yuanguang Li
C-phycocyanin is a natural blue-colored biliprotein with various biological activities. Commercial C-phycocyanin from Spirulina platensis has poor stability, which severely limits its application in food, medicine, cosmetics, and other industries. Extremophilic red algae Galdieria sulphuraria is found in hot springs with a pH of 1–3 and temperature above 40 °C. Hence, C-phycocyanin from G. sulphuraria has the potential to withstand high temperatures. This study aimed to compare the stability of C-phycocyanins from G. sulphuraria and S. platensis under different pH, temperature, stabilizer concentration, and light conditions. The results showed that C-phycocyanin from G. sulphuraria was more stable than that from S. platensis under all conditions, especially in the range of 50–65 °C, and in neutral environment of pH 7. After 30 min incubation at 60 °C and pH 7, the color preservation rate of C-phycocyanins from G. sulphuraria and S. platensis were 86.66% and 60.83%, respectively. The kinetic analysis revealed that under the influence of the stabilizer, the stabilities of both phycocyanins were improved. However, C-phycocyanin from G. sulphuraria obtained the highest mid-unfolding temperature of 73.00 °C compared with 69.81 °C for that of C-phycocyanin from S. platensis . In addition, the antioxidant capacity of two phycocyanins was similar. Therefore, C-phycocyanin from G. sulphuraria is an excellent alternative to the current commercial C-phycocyanin.
研究领域:
生物化学
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食品科学
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分子生物学
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DOI
高影响因子
BIOCONJUGATE CHEMISTRY
·
2025
·
IF 3.9
Sheng Lin,Xia Cheng,Xiyi Chen,Jinxuan He,Wenxiu An,Qiufang Bai,Qixian Chen,Lie Ma,Jianhua Hu,Yue Wang,Xiabin Lan
The refinement of dynamic molecular mechanisms regulating mRNA release kinetics represents a critical frontier in advancing the synthetic mRNA delivery systems. This study details the synthesis of an intriguing ROS-responsive cationic block copolymer (pM-pBD) via reversible addition−fragmentation chain transfer (RAFT) polymerization, employing biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) and charge-reversible (2-acryloyl)ethyl(boronic acid benzyl)diethylammonium bromide (BD) as monomeric precursors. The synthesized copolymer facilitates electrostatic-driven self-assembly with anionic mRNA. Mechanistically, the pBD block exhibits ROS-mediated charge transition, enabling stimulus-dependent molecular decomposition and promoting mRNA payload liberation, thereby establishing spatial regulation of translational activity. Furthermore, intracellular ROS modulation experiments revealed that systemic ascorbic acid administration selectively enriches reactive oxygen species within tumor microenvironments. This redox microenvironment significantly amplifies pM-pBD nanocomplex-mediated mRNA expression by an order of magnitude across multiple carcinogenic cell lines, validating the ROS-responsive characteristics of our rationally designed delivery platform. In vivo studies demonstrated the highest mRNA expression levels in tumors when aided by ascorbic acid adjuvants, despite lower tumor accumulation of mRNA compared to renal and hepatic sequestration post intravenous administration of the pM-pBD nanocomplex. This spatial expression pattern correlates with ascorbic acid-mediated intratumoral ROS accumulation, which promotes cargo release and subsequent protein synthesis of approximately 6.4-fold enhancement. Our approach, integrating redox-responsive polymer design with organ-specific pharmacological modulation, signifies a transformative advancement in targeted nucleic acid delivery. By merging stimulus-responsive materials science with tumor microenvironment biology, this methodology provides a foundation for spatially controlled mRNA expression, presenting an innovative strategy for precision oncology applications.
研究领域:
生物材料
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肿瘤学
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药剂学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
3 Biotech
·
2020
·Vol. 10
·期 4
·1-9
·
IF 1.798
Xie Xu-Ting,Zhang Xiao,Liu Yang,Chen Xian-Qiang,Cheong Kit-Leong
This study determined the composition of the monosaccharide, 3, 6-anhydrogalactose (AnGal), in red algae and explored the potential whitening activity of the extract. Using gas chromatography–mass spectrometry (GC–MS), the AnGal composition of six different species of red seaweed ( Porphyra haitanensis , Gracilaria chouae , Gracilaria blodgettii , Gracilaria lemaneiformis , Eucheuma galetinae , and Gelidium amansii ) was successfully analyzed, revealing molar ratios ranging from 1.0:1.0 to 1.0:3.1 of AnGal and galactose (Gal), respectively. Employing the tyrosinase inhibition assay, the skin-whitening effect of AnGal red seaweed polysaccharides was determined. Polysaccharides from P. haitanensis , G. chouae , and G. blodgettii as well as their degradation products showed higher tyrosinase inhibitory activity (inhibition rates 24.2–26.8%). These results suggest that the GC–MS approach could conveniently be used in quality control or for the quantitative determination of AnGal and Gal in red seaweed polysaccharides as well as exploring their potential application in cosmetic and functional food products. The findings here exhibited that red seaweed polysaccharides and their degradation products were potential ingredients for cosmeceutical industries.
研究领域:
海洋生物学
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化妆品科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 18
·14220
·
IF 5.6
Yaping Xu,Zhengwen Li,Yue Wang,Chujie Li,Ming Zhang,Haiming Chen,Wenxue Chen,Qiuping Zhong,Jianfei Pei,Weijun Chen,Guido R. M. M. Haenen,Mohamed Moalin
It has been reported that in an oxidative environment, the flavonoid2R,3R-dihydroquercetin (2R,3R-DHQ) oxidizes into a product that rearranges to form quercetin. As quercetin is a very potent antioxidant, much better than2R,3R-DHQ, this would be an intriguing form of targeting the antioxidant quercetin. The aim of the present study is to further elaborate on this targeting. We can confirm the previous observation that2R,3R-DHQ is oxidized by horseradish peroxidase (HRP), with H2O2as the oxidant. However, HPLC analysis revealed that no quercetin was formed, but instead an unstable oxidation product. The inclusion of glutathione (GSH) during the oxidation process resulted in the formation of a2R,3R-DHQ-GSH adduct, as was identified using HPLC with IT-TOF/MS detection. GSH adducts appeared on the B-ring of the2R,3R-DHQ quinone, indicating that during oxidation, the B-ring is oxidized from a catechol to form a quinone group. Ascorbate could reduce the quinone back to2R,3R-DHQ. No2S,3R-DHQ was detected after the reduction by ascorbate, indicating that a possible epimerization of2R,3R-DHQ quinone to2S,3R-DHQ quinone does not occur. The fact that no epimerization of the oxidized product of2R,3R-DHQ is observed, and that GSH adducts the oxidized product of2R,3R-DHQ on the B-ring, led us to conclude that the redox-modulating activity of2R,3R-DHQ quinone resides in its B-ring. This could be confirmed by chemical calculation. Apparently, the administration of2R,3R-DHQ in an oxidative environment does not result in ‘biotargeting’ quercetin.Keywords:2R,3R-dihydroquercetin;redox modulation;quercetin;quinone;epimerization
研究领域:
生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2025
·
IF 1.6
Zhao Fan,Zhang Wei,Chen Yuantao
The zinc-based metal–organic framework (IRMOF-3) was assembled on the surface of Galdieria sulfuraria (Gs) via a one-pot method to fabricate the composite adsorbent Gs/IRMOF-3. Results show that the material achieves optimal adsorption performance at pH = 6. The adsorption process follows the Freundlich isotherm model and pseudo-second-order kinetic model, demonstrating that it is dominated by multi-molecular layer chemical adsorption and represents a spontaneous endothermic process. This study provides a novel strategy for developing efficient and eco-friendly uranyl adsorption materials.
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产品关联
DOI
高影响因子
Journal of Functional Foods
·
2016
·
IF 3.973
Tong Di,Guijie Chen,Yi Sun,Shiyi Ou,Xiaoxiong Zeng,Hong Ye
In this work, crude polysaccharides from Gracilaria rubra (GRPS) and its three purified fractions, GRPS-1-1, GRPS-2-1 and GRPS-3-2, were obtained through hot water extraction and chromatographic purification with DEAE-52 cellulose column and Sephadex G-50 column. The three purified fractions, being homogeneous heteropolysaccharides with average molecular weights of 1310, 691, 923 kD and sulfuric radical contents of 5.96, 8.46 and 12.03%, respectively, were all composed of galactose and fucose. GRPS-2-1 and GRPS-3-2 had little toxicity to L-02 cells. Crude GRPS and its purified fractions exhibited strong scavenging activities against ABTS and superoxide radicals and lipid peroxidation inhibition. At a cellular level, GRPS-3-2 showed the strongest protective effect on H 2 O 2 -induced oxidative injury in PC12 cells. Moreover, GRPS-3-2 exhibited a much stronger immunostimulating activity on RAW264.7 cells than GRPS-1-1 and GRPS-2-1. The results suggested that GRPS, especially GRPS-3-2, could be developed as a promising functional food supplement.
研究领域:
海洋生物学
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药理学
·
生物化学
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产品关联
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2022
·Vol. 70
·期 2
·697-706
·
IF 2.724
Shijin Wu,Bijiao Wang,Hongde Yan
Asymmetric reduction of ( R )-carvone and ketoisophorone by an engineered ene-reductase from Galdieria sulphuraria (GsOYE) combined with glucose dehydrogenase for NADPH regeneration were studied. A semi-rational protein engineering was used to enhance the activity and selectivity of GsOYE. Upon the sequence alignment and molecular docking results, two amino acid residues at positions 66 and 270 were selected as saturation mutation sites. Finally, a single substitution variant of GsOYE-N270A with complete conversion (100%) and diastereoselectivity (de p >99%) for reduction of ( R )-carvone and a double substitution variant GsOYE-Y66P/N270H with improved stereoselectivity for reduction of ketoisophorone were obtained.
研究领域:
生物化学
·
蛋白质工程
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催化
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）