LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Linling Li,Wenxin Guo,Lu Wang,Shuiyuan Cheng,Hua Cheng
Chitosan (CTS) exhibits notable moisturizing and film-forming capabilities, whereas nano selenium (SeNPs) demonstrates antioxidant and antibacterial properties. In the present study, a CTS/Se film-forming agent was prepared by dissolving CTS, glycerol, and SeNPs in 1% (v/v) glacial acetic acid through heating and ultrasonic treatment. The results revealed that SeNPs fused with CTS via intermolecular forces, thereby enhancing the tensile strength of the CTS film. The water contact angle of the CTS film increased with an increase in SeNPs concentration. The thermal stability and water retention properties of CTS/Se were superior than CTS. In a storage experiment, the content of soluble solids in tomatoes coated with CTS/Se was higher than that in tomatoes coated with CTS and the control. The CTS/Se coating inhibited gas exchange both inside and outside the film, leading to increased activities of antioxidant enzymes. This reduced the oxidation of vitamin C in the tomatoes and decreased the content of malondialdehyde. The CTS/Se film inhibited the growth of bacteria on the tomato surface and mitigated the decline of aldehyde, alcohol and ketone aroma compounds. Consequently, the CTS/Se coating alleviated the softening, aging and rotting of tomatoes. Feeding experiments conducted on mice verified the food safety of the CTS/Se.
研究领域:
食品科学
·
植物病理学
·
材料科学
✓ 已验证
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Xiaoxue Zhang,Mengmeng Zhong,Rui Zhou,Wenxiu Qin,Youbin Si
Microbial reduction of Se(IV) to Se(0) is extremely important for the detoxification of selenium oxyanion, but the reductive mechanism is still controversial. In this study, the reduction of Se(IV) by Shewanella oneidensis MR-1 and Shewanella putrefaciens was systematically investigated. The results showed that excessive Se(IV) severely inhibited the growth of microorganisms and Se(IV) reduction. The extracellular accumulation of Nano-Se(0) particles was shown to be approximately 250–650 nm in diameter. Riboflavin facilitated Se(IV) reduction, while rotenone , as an electron inhibitor, could completely inhibit Se(IV) reduction, validating the extracellular electron transport process for biosynthesized Nano-Se(0). Importantly, the amorphous Nano-Se(0) synthesized by S. oneidensis MR-1 were mixtures of helical chains and Se 8 rings, while those synthesized by S. putrefaciens were mainly composed of helical chains. FTIR results demonstrated that Nano-Se(0) particles were wrapped by some proteins, organic substances and lipids , exhibiting superior dispersibility and biocompatibility. Biogenic Nano-Se(0) particles can also be considered for technological applications in various fields.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Zhijia Zhang,Shujun Huang,Xin Huang,Tianbing Zhou,Tao Zhang,Yuxin Xu,Mengmeng Li,Jialing Wang,Shuai Wang,Zhan Hu,Ranfeng Sun,Dong Li
The principal disease impacting pepper production is Phytophthora blight , which nano‑selenium (nano-Se) controls well but has high storage requirements and low heat resistance. Varying pectin amounts yielded pectin-modified nano-Se (nano-Se@PT) with 0.1 %, 0.5 %, 1 %, and 2 % concentration. As pectin content grew, nano-Se@PT particle size reduced, zeta potential value rose, and thermogravimetric temperature climbed. The incorporation of nano-Se@PT can improve its stability and thermal resistance at 40 °C and 70 °C. Nano-Se@PT outperformed nano-Se in fungicidal and Phytophthora capsici inhibition. In vitro antifungal experiments showed that nano-Se@PT could significantly inhibit mycelium growth with an EC 50 of about 4 mg/L. The particles adsorbed on the surface of mycelium, inducing death by destroying cell membranes, mitochondria, and nuclei. Non-targeted metabolomics analysis showed that nano-Se@PT treatment disrupted the mycelium growth and metabolism and changed amino acids, nucleotides, and sugar levels. In vivo experiments, 2 % nano-Se@PT had the lowest disease index and the best preventive therapy. Rhizosphere soil microbial analysis showed that nano-Se@PT recruited beneficial microorganisms such as Bacteroides, Lactobacillus, and Bifidobacterium in pepper plants, improving their Phytophthora capsici resistance and growth. The findings suggest that nano-Se@PT might be a unique pepper disease control technique and shed light on plant fungal disease management.
研究领域:
植物病理学
·
微生物学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Jingyuan Wang,Ronggang Zhai,Yifan Ma,Haoyu Chen,Danyang Jing,Huaiyu Yang,Yi Wang
Owing to their small size, morphology and release modification properties, nanopesticides are considered promising alternative strategies for enhancing biological activity and minimizing pesticide losses. In this study, we used a colloidal self-assembly method to develop a morphology-stable, regularly rod-shaped nanoselenium pesticide carrier (NSer), which was further modified with chitosan. After loading penthiopyrad (PEN), the biological activity of NSer@PEN and its impact on the physiological and biochemical processes of plants were further compared with those of spherical nanoselenium pesticides (NSes@PEN) and commercial materials (20 % PEN SC). The biological activities were quantified through the EC 50 values, which revealed that NSer@PEN (0.71 mg/L) and NSes@PEN (1.09 mg/L) exhibited significantly greater activity against Colletotrichum orbiculare Arx compared to 20 % PEN SC (2.70 mg/L). Moreover, through further investigation into the impact of nanopesticides on plant root exudates, Fourier transform infrared spectroscopy (FTIR) and two-dimensional correlation spectroscopy (2D-COS) analysis revealed that the ketone C O bond exhibited the strongest binding affinity, and the C O bond of phenols contributed significantly to the binding of cucumber root exudates induced by NSer@PEN, resulting in a mild response of the plant. The morphology-dependent nanoselenium carriers developed in this work are expected to enhance biological activity and reduce plant stress caused by pesticides, tackling one of the application challenges of pesticides.
研究领域:
植物生理学
·
农业科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Green Processing and Synthesis
·
2021
·Vol. 10
·期 1
·178-188
·
IF 2.83
Su Wen,Yang Hui,Wang Chuang
Nano-selenium was synthesized using lemon juice as a reducing agent. The experiments showed that pH value affected greatly the shape and the size of the prepared nano-selenium. At pH 9, lemon juice could reduce 50 mmol/L of selenite ions to nano-selenium with particle size between 50 and 90 nm, which was spherical and well dispersed. Lemon juice acted as both a reducing agent and a stabilizer in the synthesis of nano-selenium, in which the chemical interaction between biomolecules and the nano-selenium surface was the basis for the stable existence of nano-selenium. The selenite concentration influenced the formation of nano-selenium, and a low selenite concentration was beneficial to obtain small particles. The achieved nano-selenium exhibited a strong antioxidant activity positively related to concentration. The comparative study showed that the antioxidation of nano-selenium is weaker than that of vitamin C but higher than that of lemon juice.
Graphical abstract
Green synthesis of nano-selenium from lemon juice, selenium nanoparticles coated with macromolecules, and antioxidant activity.
研究领域:
生物化学
·
纳米生物学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Mengdi Zhang,Jindong Gao,Md. F. Kulyar,Wanhe Luo,Guodong Zhang,Xiaoqi Yang,Tianguang Zhang,Haihang Gao,Yuxuan Peng,Jiabin Zhang,Muhammad Altaf,Samah Attia Algharib,Donghai Zhou,Jianzhong He
Acute renal failure is a common clinical disease in canines, affecting antioxidant levels and decreasing the body's resistance. This study aims to explore the therapeutic mechanism of Nano-selenium in acute renal failure. The histopathological and imaging changes of kidney tissue were observed with the gene and protein expression levels of Keap1, Nrf2, HO-1, and NQO1 in the kidney. According to our findings, adding nano-selenium can effectively reduce the concentration of CRE and BUN in blood and kidney tissues. It increased the activity of GSH-PX and SOD by an effective reduction of MDA. Through pathological and imaging observations, it was found that adding nano-selenium could improve the kidney tissue structure of acute renal failure. The results of the RT-qPCR experiment showed that after the addition of nano-selenium, the mRNA expression of the Keap1 gene decreased significantly. In contrast, the mRNA expression of the Nrf2, HO-1, and NQO1 genes increased significantly. The experimental results were further verified by western blot and immunohistochemical analysis. Hence, the nano-selenium intervention improved kidney function and increased antioxidant levels in canines suffering from acute renal failure with the involvement of the Keap1-Nrf2/ARE signaling pathway.
研究领域:
兽医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Xian Guo,Borui Zhang,Yutong Chen,Zhi Jia,Xiaoyu Yuan,Li Zhang,Jie Liu,Yanan Liu
Clinical trials based on a single molecular target continue to fail, and the adverse effects of Aβ protein aggregation and neuroinflammation need to be solved and treatment of Alzheimer's disease. Herein, by designed a nano-sized flower mesoporous selenium transport carrier (Met@MSe@Tf) with high enzyme-like activity, metformin (Met) was loaded, and transferrin (Tf) was modified to bind to transferrin receptor to promote receptor-mediated transport across the BBB. In the AD lesion environment, with the acidic environment response dissociation, promote the release of metformin by nanoflower to achieve therapeutic effect in the brain lesion site. Metformin, a major anti-diabetic drug in diabetic metabolism, has been found to be a promising new therapeutic target in neurodegenerative diseases. Further studies showed that the metformin drug release from the designed and synthesized transport nanoparticles showed high intrinsic activity and the ability to degrade the substrate involved, especially the degradation of Aβ deposition in the cortex and hippocampus, increased the phagocytosis of microglia, thus relieving neuroinflammation simultaneously. Collectively, in vivo experiments demonstrated that Met@MSe@Tf significantly increased the number of NeuN-positive neurons in the hippocampus of AD mice, promoted neurovascular normalization in the brain, and improved cognitive dysfunction in AD transgenic AD mice. Thus, it provides a preclinical proof of concept for the construction of a highly modular accurate drug delivery platform for Alzheimer's disease.
研究领域:
药理学
·
神经科学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2024
·
IF 4.5
Min Wang,Tianming Dai,Dan Zhu
Myocardial infarction, commonly known as a heart attack, is the irreversible necrosis of heart muscle secondary to prolonged ischemia. In this study, we developed multifunctional injectable hydrogel for promising treatment of myocardial infarction that combination with mesenchymal stem cells (MSCs) and nanoselenium-ruthenium (Se-Ru NPs)-loaded collagen (COL)/hyaluronic acid (HA) (termed as Hydrogel). Efficient analytical and spectroscopic methods systematically analyzed the physicochemical analysis of developed Se-Ru NPs. In vitro biocompatibility of rat cardiomyoblast (H9c2) assay showed that hydrogel has excellent cell viability, as determined by CCK-8 and LDH assays, which defined the appropriate use of hydrogels for MI therapy. The migration assay displayed ∼95% migration for Hydrogel/Se-Ru@MSCs compared to the rest of the groups on the H9c2 cells. The study demonstrated that Hydrogel/Se-Ru@MSCs exhibited a 4-fold decrease of inflammatory marker expressions than LPS-induced H9c2 and RAW 264.7 cells. Further, the apoptosis confirmed that established Hydrogel/Se-Ru decreased the LPS-induced cell death. When the blended Hydrogel/Se-Ru group was injected into MI SD rat, in vivo studies revealed enhanced cardiac function over improved LV wall thickness and significantly reduced infarct size compared to the MI group. The outcomes established that the developed injectable hydrogels significantly impacted myocardial infarction recovery.
研究领域:
生物材料学
·
心血管医学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhiying Shao,Xiaoyi Jiang,Qinlu Lin,Simiao Wu,Siming Zhao,Xinyang Sun,Yunhui Cheng,Yong Fang,Peng Li
The presence of potentially toxic elements and compounds poses threats to the quality and safety of fruit juices. Among these, Hg(II) is considered as one of the most poisonous heavy metals to human health. Traditional chitosan-based and selenide-based adsorbents face challenges such as poor adsorption capacity and inconvenient separation in juice applications. In this study, we prepared nano‑selenium functionalized chitosan gel beads (nanoSe@CBs) and illustrated the synergistic promotions between chitosan and nanoSe in removing Hg(II) from apple juice. The preparation conditions, adsorption behaviors, and adsorption mechanism of nanoSe@CBs were systematically investigated. The results revealed that the adsorption process was primarily controlled by chemical adsorption. At the 0.1 % dosage, the adsorbent exhibited high uptake, and the maximum adsorption capacity from the Langmuir isotherm model could reach 376.5 mg/g at room temperature. The adsorbent maintained high adsorption efficiency (> 90 %) across a wide range of Hg(II) concentrations (0.01 to 10 mg/L) and was unaffected by organic acids present in apple juice. Additionally, nanoSe@CBs showed negligible effects on the quality of apple juice. Overall, nanoSe@CBs open up possibilities to be used as a safe, low-cost and highly-efficient adsorbent for the removal of Hg(II) from juices and other liquid foods.
研究领域:
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Changfang Lu,Yutong Wang,Wei Zhang,Yu Zhang,Yinyin Chen,Gang Xiang,Xianxiang Wang
The crosslinking of glutaraldehyde (GA) and nano-selenium coated chitosan (CS-SeNPs) afforded composites (GA–CS–SeNPs) which exhibit new properties. In addition to having mimic enzyme activity of CS-SeNPs, these composites exhibit fluorescence, which CS-SeNPs did not possess. The GA–CS–SeNPs can catalyze the chromogenic process of oxidation of substrate TMB at 20 °C and pH of 3.5, and under the excitation wavelength of 440 nm, the composites can emit strong fluorescence. The presence of mercury (II) (Hg 2+ ) inhibited the color process of catalytic oxidation of 3,3,5,5, -tetramethylbenzidine (TMB) and enhanced the fluorescence intensity of the composites to some extent. Therefore, using the fluorescence and mimicking enzyme properties of GA–CS–SeNPs, a fluorescent and colorimetric (dual-mode) determination of Hg 2+ was developed with good selectivity and high sensitivity. The linear determination ranges of Hg 2+ were 0–55 μM and 0–50 μM for fluorescent and colorimetric assays, respectively, and the limits of detection (LOD) for the dual-mode assay were as low as 0.34 μM and 0.40 μM, respectively. In addition, this new method was successfully implemented for dual-mode determination of Hg 2+ in real samples.
研究领域:
分析化学
·
环境科学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Jianshuang Jiao,Juan Yu,Haiyu Ji,Anjun Liu
In the present study, the previously obtained macromolecuar-weight Astragalus polysaccharide (average molecular weight of 1.61 × 10 6 Da) was used as a stabilizer and dispersing agent for nano-composites preparation by modifying selenium nanoparticles, and then the anti-hepatoma activity on HepG2 cells was investigated as well. Results showed that the nano-composites were obtained under polysaccharide concentration of 2 mg/mL and selenium/polysaccharide mass ratio of 1:15, and exhibited symmetrical spheroid with an average diameter of 62.3 nm, which has a good stability for 35 days at 4 °C. Furthermore, the in vitro anti-hepatoma experiments demonstrated that the composites could significantly inhibit the proliferation of HepG2 cells in a dose-dependent manner, and could induce the morphological changes, arrest the cell cycle in S phase, finally triggering HepG2 cells apoptosis through mitochondrial pathway. These data revealed that the composites had the potential to be a novel therapeutic drug or adjuvant for hepatoma-bearing patient treatments.
研究领域:
药学
·
癌症生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 17
·3523
·
IF 4.967
Fengqin Wang,Man Du,Lixia Kai,Shuai Du,Weilian Hu,Yizhen Wang,Yuanzhi Cheng
To provide a safe and effective supplement of the essential trace element selenium, we focused on the biosynthesis of nanoselenium (SeNPs) via probiotics. A novel kind of exopolymer-functionalized nanoselenium (SeEPS), whose average size was 67.0 ± 0.6 nm, was produced byBacillus subtilisSR41, whereas the control consisted of exopolymers without selenium (EPS). Chemical composition analysis, Fourier transform infrared (FTIR) spectroscopy and high-performance liquid chromatography (HPLC) confirmed that SeEPS and EPS shared similar polysaccharide characteristic groups, such as COO- and C=O, and contained not only 45.2–45.4% of sugars but also 23.5–24.7% of proteins and some lipids. Both SeEPS and EPS were primarily composed of mannose, amino glucose, ribose, glucose and galactose. Furthermore, to identify the biologically active component of SeEPS, three kinds of selenium particles with different stabilizers [Se(0), bovine serum albumin-Se and EPS-Se] were synthesized chemically, and their ability to scavenge free radicals in vitro was compared with that of SeEPS and EPS. The results revealed that EPS itself exhibited weak superoxide and hydroxyl radical scavenging abilities. Nevertheless, SeEPS had superior antioxidant properties compared to all other products, possibly due to the specific structure of SeNPs and exopolymers. Our results suggested that exopolymer-functionalized SeNPs with specific monosaccharide composition and structure could eventually find a potential application as an antioxidant.Keywords:exopolymer-functionalized nanoselenium;monosaccharide composition analysis;free radical scavenging ability
研究领域:
生物技术
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）