JOURNAL OF NANOPARTICLE RESEARCH
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2015
·Vol. 17
·期 10
·1-10
·
IF 2.184
Chen Daquan,Wang Guohua,Song Weiguo,Zhang Qiang
Most anticancer drugs cannot kill cancer stem cells (CSCs) effectively, which lead to the failure of anticancer chemotherapy, such as relapse and metastasis. In this study, we prepared a multifunctional oligosaccharides of hyaluronan (oHA) conjugates, oHA-histidine-menthone 1,2-glycerol ketal (oHM). The oHM conjugates possess pH-sensitive menthone 1,2-glycerol ketal (MGK) as hydrophobic moieties and oHA as the target of CD44 receptor. Anticancer drugs, curcumin(Cur) and paclitaxel(PTX), were loaded into oHM micelles via self-assembly. Then, oHM micelles were mineralized through controlled deposition of inorganic calcium and phosphate ions on the nanoparticular shell via a sequential addition method to fabricate the “nano-eggs.” The formed nano-eggs had a smaller size (120.6 ± 4.5 nm) than oHM micelles (158.6 ± 6.4 nm), indicating that mineralization made the appearance of compact nanoparticles. Interestingly, when the nano-eggs were put into the acidic conditions (pH 6.5), their outer shell(inorganic minerals) will be destroyed with the larger size, while the “nano-eggs” were stable under pH 7.4. For both nano-eggs and oHM micelles, the Cur and PTX were released in a sustained manner depending on the pH of the solution. However, the nano-eggs showed much lower released than the oHM micelles due to the dissolution of the inorganic minerals and pH-sensitive ketal at mildly acidic environments (pH 6.5). In vivo study, the nano-eggs could get to the tumor site more effectively than oHM micelles. CSCs were sorted by a side population assay from MDA-MB-231 breast cancer cell lines over-expressing CD44 receptors. Antitumor activity was also evaluated on MDA-MB-231 xenografts in nude mice. The antitumor efficacy indicated that nano-eggs with co-delivery of Cur and PTX produced the strongest antitumor efficacy, and nano-eggs showed strong activity against cancer stem cells. These double pH-sensitive nano-eggs may provide a promising strategy for drug delivery to both cancer cells and cancer stem cells.
研究领域:
药学
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肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Fuge Niu,Yu Jiao,Rui Zhang,Jiamei Fan,Christos Ritzoulis,Weichun Pan,Ang Li
This work studied the quantitation of myofibrillar protein isolated from giant squid by three methods (Bradford, biuret, and direct ultraviolet absorbance). The results were examined in relation to compositional size exclusion chromatography, static light scattering, zeta-potential and thermal analysis; comparisons between the apparent vs. the true protein concentration revealed the existence of disk-like nano protein particles (NPP) with a height of 2–3 nm, diameters ranging from several tens nm to 140 nm, and fractal dimensions d f of less than 1.3. In order to probe the heterogeneity of NPP particles, their properties were studied under consecutive dilutions: the d f decreased from 1.265 to 1.087, the zeta-potential increased from −4.55 mV to −1.83 mV, the denaturation temperature reduced from 63.9 °C to 58.6 °C, and the endothermic enthalpy reduced from 0.529 J s −1 to 0.362 J s −1 . In addition, ca. 90% protein molecules in solution were aggregated to form NPP.
研究领域:
食品科学
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生物物理学
·
蛋白质化学
✓ 已验证
DOI
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2023
·
IF 2.5
Tian Wang,Hong-Mei Li,Bao-Ying Wen,Rajapandiyan Panneerselvam,Yue-Jiao Zhang,An Wang,Fan-Li Zhang,Shangzhong Jin,Jian-Feng Li
Dioxin is a highly toxic and carcinogenic pollutant created during industrial production and waste incineration . Pollutant monitoring has made extensive use of surface-enhanced Raman spectroscopy (SERS) as a quick, non-destructive, sensitive, and affordable analytical method. However, the fabrication of a SERS substrate with ultrahigh sensitivity is a challenging task, the detection cost is expensive. Currently, these are the challenges faced by SERS technique for the detection of dioxin pollutants. In this paper, we present Au nanocakes (Au NCs) as an easy-to-build SERS substrate for sensitive SERS detection of dioxins in real samples. Based on three-dimensional finite-difference time-domain (3D-FDTD) calculations, the enhancement factor of the substrate following aggregation is approximately 10 10 . Using a portable Raman spectrometer, the limit of detection for dioxins such as 1-chloro-dibenzo-p-dioxin (1-CDD), 2,8-dichloro-dibenzo-p-dioxin (2,8-DCDD), and 2,3,7-trichloro-dibenzo-p-dioxin (2,3,7-TrCDD) in clean water reached as low as 5, 5, and 10 ng/mL, respectively. As a real-world application, the same toxic pollutants were detected in sewage water samples. Our findings could lead to the development of novel SERS-based sensors for the rapid detection of dioxins in real-world scenarios. Moreover, a portable Raman spectrometer is used to detect the pollutants, which is easy to use and inexpensive.
研究领域:
环境科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Silin Du,Guangyu Ma,Xiang Li,Minghao Chao,Liying Fan,Zhiqiang Zhao,Rongze Tang,Junjie Li,Chaonan Jing,Yufan Liu,Dehong Yu,Zhao Liu,Fenglei Gao
To overcome the resistance of tumor cells to death through a single pathway, PANoptosis, a novel synergistic mode of cell death, can simultaneously activates pyroptosis, apoptosis, and necroptosis. Most current strategies for inducing PANoptosis focused on amplifying reactive oxygen species (ROS) through exogenous stimuli, while the regulatory role of tumor cell metabolic characteristic in this process has long been overlooked. We propose a ROS-Metabolism dual axis synergistic induction of PANoptosis, simultaneously enhancing antitumor immune responses, and construct a novel bionic nanoplatform named CVOVM. This platform consists of CoVO x nanocakes and the glutamine transporter inhibitor V-9302, with 4T1 cell membranes as camouflage. In tumors, CVOVM initiates a series of catalytic reactions: CoVO x promotes numerous ROS generation, while V-9302 disrupts glutamine metabolism, depleting glutathione (GSH) and nicotinamide adenine dinucleotide (NAD + ), thereby triggering the assembly of PANoptosome primarily composed of NLRP3 and NLRC5, which initiates pyroptosis, apoptosis, and necroptosis. The occurrence of PANoptosis promotes immunogenic cell death (ICD) to remodel tumor immunosuppressive microenvironment, and enhances immunotherapy and inhibits lung metastasis. Overall, we present a previously unreported mechanis to achieve the synergistic induction of PANoptosis through ROS amplification and metabolic inhibition, enhancing antitumor immunity, and potentially providing new opportunities and possibilities for combination cancer therapy.
研究领域:
癌症研究
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纳米医学
·
免疫治疗
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细胞死亡机制
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活性氧信号通路
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肿瘤代谢
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仿生纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xu Liu,Yuan Zhao,Cong Gao,Xiaohuan Sun,Shixin Li,Jie Han
Understanding the unique role of supramolecular chirality in nano-protein/cell interactions, as opposed to molecular chirality, is crucial for elucidating the origin of life. However, this aspect has received inadequate attention. In this study, L/D-glutamic acid-based amphiphiles (L/D-GluC16), which possess molecular chirality, were synthesized. Additionally, through the manipulation of solvent effects, left/right-handed helical nanofibers ( M / P -GluC16), endowed with supramolecular chirality, were assembled from L/D-GluC16. With the aid of molecular dynamics (MD) simulations, the interaction between M / P -GluC16, L/D-GluC16, and a model protein (bovine serum albumin, BSA) was investigated at the thermodynamic and spatial orientational levels. Combined with the experimental result of protein adsorption efficiency, a more favorable chirality-dependent binding of M / P -GluC16 to protein compared to L/D-GluC16 was confirmed. Furthermore, driven by the binding-induced conformational changes and subsequent functional disruptions of proteins, M / P -GluC16 exhibited a greater chirality-specific cancer cell inhibitory efficacy compared to L/D-GluC16, highlighting the more significant impact of supramolecular chirality on nano-protein/cell interactions than molecular chirality.
研究领域:
纳米技术
·
生物物理学
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细胞生物学
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计算生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）