TALANTA
·
2017
·
IF 4.162
Fangfang Li,Xuan Hu,Fengyi Wang,Baozhan Zheng,Juan Du,Dan Xiao
A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO 2 NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA and NP surface, which induced complete quenching in the FAM-DNA fluorescence due to a photo induced electron transfer (PET) process. It was found that ATP can readily displace adsorbed DNA from nanoceria surface because of the stronger coordination ability of ATP with nanoceria, and the nanoceria-based competitive binding resulted in over 7-fold fluorescence enhancement. Over a wide range from 0.1 nM to 1.5 μM, a good linear relationship between the fluorescence intensity and the concentration of ATP was obtained and the detection limit was estimated to be as low as 54 pM. This method was successfully used to analyze ATP in a single drop of blood and human urine.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Shi Gang Liu,Siyi Liu,Shujuan Yang,Qian Zhao,Jiehong Deng,Xingbo Shi
Hydrogen peroxide (H 2 O 2 ) is widely used as disinfectant and bleaching agent in food industry, but excessive amounts of H 2 O 2 residue in foods can cause serious adverse effects on human health. Herein, a fluorescent sensing strategy for rapid detection of H 2 O 2 in foods is described based on a nanohybrid system of nanoceria and carbon dots (CDs). Nanoceria can reduce the fluorescence intensity of CDs, but the presence of H 2 O 2 further quenches the fluorescence remarkably, which is attributed to the H 2 O 2 -promoted electron transfer. Based on H 2 O 2 concentration-dependent fluorescence quenching of CDs/nanoceria, the fluorescence method for H 2 O 2 determination is established with a linear range of 0.1–100 μM and a detection limit of 0.047 μM. Meanwhile, the smartphone is utilized as a portable platform for facile and low-cost detection of H 2 O 2 , offering a promising method for rapid sensing of H 2 O 2 on site. The fluorescent strategy is applied to H 2 O 2 residue detection in milk, dried bean curd, and chicken feet samples successfully, suggesting its great potential for applications in food and agricultural fields.
研究领域:
食品科学
·
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2021
·Vol. 11
·期 5
·1168
·
IF 5.076
Yuanpei Lan,Xuewen Xia,Junqi Li,Xisong Mao,Chaoyi Chen,Deyang Ning,Zhiyao Chu,Junshan Zhang,Fengyuan Liu
Oxygen vacancies (OVs) have critical effects on the photoelectric characterizations and photocatalytic activity of nanoceria, but the contributions of surface OVs on the promoted photocatalytic properties are not clear yet. In this work, we synthesized ceria nanopolyhedron (P-CeO2), ceria nanocube (C-CeO2) and ceria nanorod (R-CeO2), respectively, and annealed them at 600 °C in air, 30%, 60% or pure H2. After annealing, the surface OVs concentration of ceria elevates with the rising of H2concentration. Photocatalytic activity of annealed ceria is promoted with the increasing of surface OVs, the methylene blue photodegradation ratio with pure hydrogen annealed of P-CeO2, C-CeO2or R-CeO2is 93.82%, 85.15% and 90.09%, respectively. Band gap of annealed ceria expands first and then tends to narrow slightly with the rising of surface OVs, while the valence band (VB) and conductive band (CB) of annealed ceria changed slightly. Both of photoluminescence spectra and photocurrent results indicate that the separation efficiency of photoinduced electron-hole pairs is significantly enhanced with the increasing of the surface OVs concentration. The notable weakened recombination of photogenerated carrier is suggested to attribute a momentous contribution on the enhanced photocatalytic activity of ceria which contains surface OVs.Keywords:surface oxygen vacancies;nanoceria;photocatalytic property;band gap;photoinduced carriers
研究领域:
材料科学
·
化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Oncology
·
2023
·
IF 4.7
Zhang Lidong,Liu Xiaoguang,Mao Yinghua,Rong Shu,Chen Yonghong,Qi Yong,Cai Zhipeng,Li Hong
Tumor hypoxic environment is an inevitable obstacle for photodynamic therapy (PDT) of melanoma. Herein, a multifunctional oxygen-generating hydrogel loaded with hyaluronic acid-chlorin e6 modified nanoceria and calcium peroxide (Gel-HCeC-CaO2) was developed for the phototherapy of melanoma. The thermo-sensitive hydrogel could act as a sustained drug delivery system to accumulate photosensitizers (chlorin e6, Ce6) around the tumor, followed by cellular uptake mediated by nanocarrier and hyaluronic acid (HA) targeting. The moderate sustained oxygen generation in the hydrogel was produced by the reaction of calcium peroxide (CaO2) with infiltrated H2O in the presence of catalase mimetic nanoceria. The developed Gel-HCeC-CaO2 could efficiently alleviate the hypoxia microenvironment of tumors as indicated by the expression of hypoxia-inducible factor -1α (HIF-1α), meeting the “once injection, repeat irradiation” strategy and enhanced PDT efficacy. The prolonged oxygen-generating phototherapy hydrogel system provided a new strategy for tumor hypoxia alleviation and PDT.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 13
·9997
·
IF 4.6
Zhendong Fu,Jiahe Qiu,Ping Gong,Danhong Zhang,Liping Wang
The precise and sensitive quantification of total antioxidant capacity (TAC) is indispensable for evaluating the quality of foods rich in antioxidants. In this investigation, a novel nanozyme exhibiting robust oxidase activity was synthesized via a sonochemical doping process utilizing copper ions and the modification of 2-propylimidazole onto nanoceria. Subsequently, a CeO2@Cu-PrIm/ox-TMB assay system was successfully formulated, furnishing a practical and highly sensitive analytical tool for TAC determination. The colorimetric sensor exhibited a linear response over a concentration range of 1 μM to 70 μM, accompanied by a limit of detection (LOD) of 1.26 μM under meticulously screened conditions. This assay displayed substantial practical utility for TAC analysis in fruits, attributed to its exceptional accuracy and selectivity. This research endeavor may offer a novel direction for the design of nanozymes and colorimetric biosensors possessing heightened oxidase activity, thereby advancing the field of analytical chemistry and food science. Schematic depicting the reaction system of CeO2@Cu-PrIm/ox-TMB assay for the determination of TAC.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xueran Shi,Yingbo Cao,Lin Cong,Tiance Gu,Jing Wang,Qian Han
Due to serious harm of the tetracycline (TC) residue to human health, here a multifunctional nanoceria in situ-derived porphyrin heterogeneous framework (CeO 2 -POF) was innovatively applied to electrochemiluminescence (ECL) and colorimetric dual-mode sensing platform for TC detection. Concretely, the CeO 2 -POF fully interprets a “kill three birds with one stone” strategy. Firstly, CeO 2 -POF integrated ECL luminophore (“bird” one) and co-reaction accelerator (“bird” two), obtaining a 1.6-fold stronger ECL emission than that of tetrakis (4-carboxyphenyl) porphyrin (TCPP) ligand. Secondly, compared with two self-separated signal probes, the single CeO 2 -POF heterostructure exhibits enhanced ECL and peroxidase-like properties (“bird” three). CeO 2 -POF showed high catalyst activity toward H 2 O 2 and TMB, with color changes monitored via smartphone. Under optimized conditions, the dual-mode sensor for TC achieved a satisfied ECL detection limit of 0.57 pM and colorimetric detection limit of 0.52 nM. Compared with the commercial ELISA kit, the dual-mode sensing strategy exhibited satisfactory recovery in foods.
研究领域:
分析化学
·
食品安全
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemCatChem
·
2018
·Vol. 11
·期 2
·737-743
·
IF 4.674
Wenjing Guo,Mian Zhang,Zhangping Lou,Min Zhou,Peng Wang,Hui Wei
Graphical Mn 1 Ce 5 solid solution with the highest Mn content exhibited the highest peroxidase mimicking activity. Both the surface oxygen (O β ) and the synergistic effect between Ce and Mn played key roles in the peroxidase mimicking activity of the Mn doped nanoceria, demonstrating Mn doped nanoceria as promising peroxidase mimics and also provides an effective and facile strategy for modulating the catalytic activity of nanozymes. Nanoceria has been developed as a biocompatible and robust enzyme mimic (called ceria nanozyme) and applied in bioanalysis and therapeutics. However, its catalytic activity such as peroxidase mimic is still moderate, which in turn has limited its promising applications. To tackle this issue, herein we reported an effective strategy to modulate the peroxidase mimicking activity of nanoceria by doping transition metals. We discovered that doping of the first row of transition metals ( i. e ., Mn, Fe, Co, Ni, and Cu) modulated the activity of nanoceria and with the same doping ratio the activities followed the below order: Mn 1 Ce 10 >Co 1 Ce 10 >Fe 1 Ce 10 >Cu 1 Ce 10 >CeO 2 >Ni 1 Ce 10 . By further varying the Mn/Ce ratio, Mn 1 Ce 5 with the highest peroxidase mimicking activity was obtained (13-fold higher compared with the pristine CeO 2 ). Meanwhile we found that the homogenous doping of Mn into the nanoceria matrix without phase segregation ensured the formation of Mn 1 Ce 5 solid solution. From our experiments and discussion, we concluded that the surface oxygen (O β ) and the synergistic effect between Ce and Mn played key roles in the enhancement of Mn 1 Ce 5 peroxidase mimicking activity. This study not only demonstrates that Mn-doped nanoceria exhibits higher active peroxidase mimics but also provides a promising strategy to modulate the catalytic activity of nanozymes.
研究领域:
生物分析
·
纳米医学
·
材料科学
·
酶学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 8
·3563-3572
·
IF 2.7
Wendi Lv,Ziyuan Gao,Huijuan Wu,Lizhi Liu,Huiyong Wang,Baozhan Zheng,Juan Du,Yong Guo
Highly efficient and low-cost materials for the degradation of organic dyes in wastewater remain the focus of environmental protection and water pollution control, in which, nanozymes have been at the forefront for the development of a new platform and aroused more and more attention. In this work, a nanoceria@shaddock peel-derived nanocomposite (Ce@CSP) with excellent peroxidase-like performance in methyl violet (MV) degradation as well as ascorbic acid (AA) detection was synthesized by a simple one step in situ pyrolysis of cerium ammonium nitrate (Ce(NH4)2(NO3)6) and pretreated shaddock peel. By means of the cooperative-reinforcing peroxidase-like activity of the porous carbon material originating from shaddock peel (CSP) and the loaded CeO2 nanoparticle, the obtained nanozyme Ce@CSP displayed a superior catalytic ability in the catalyzed oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. Specifically, a synergetic absorbent property was also demonstrated by the porous carbon material, bringing the target molecules in close proximity to the nanozyme catalyst and therefore enabling effective decoloration of MV and colorimetric detection of AA with a linear range of 1.6–17.3 μM and a detection limit of 1.07 μM. The current work has realized effective decoloration of MV in real water samples and provided a wide range of potential applications of low-cost and eco-friendly materials in organic dye treatment.
研究领域:
环境科学
·
材料科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lei Zhan,Jia Ying Zhou,Chun Mei Li,Yuan Fang Li,Cheng Zhi Huang
Rapid and reliable detection of foodborne pathogens is a key requirement for food safety. Traditional rapid ELISA methods assessed by the ability of horseradish peroxidase to oxidize a chromogenic substrate such as 3, 3′, 5, 5′-tetramethylbenzidine in the presence of H 2 O 2 may result in the high rate of negative responses because of the instability of H 2 O 2 and product. Here, we report the use of redox active nanoceria as colorimetric indicator in immunoassay taking Staphylococcus aureus as a model. The nanoceria is fully water-soluble and can be stored as least 90 days in the refrigerator while maintaining the same performance. Moreover, it does not require external H 2 O 2 , as glucose oxidase used in the immunoassay can catalyze the oxidation of glucose to produce H 2 O 2 in situ , which is more robust in signal readout. Taken together, these results demonstrate that nanoceria are potentially suitable as colorimetric substrate in the development of novel enzyme immunoassays.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Jinyi Zhang,Jie Wang,Jing Liao,Yao Lin,Chengbin Zheng,Juewen Liu
Catalytic cascades have drawn much attention by avoiding the isolation of intermediates and due to high atom economy. Yet, developing an efficient, one-pot biocatalytic cascade remains challenging. Combined with the selectivity of biological enzymes and tunable activity of nanozymes, we herein demonstrate an effective bio-nanozyme cascade formed by glucose oxidase (GOx) and in situ-generated nanoceria. The prepared H2O2–nanoceria complex shows strong oxidative activity for common chromogenic substrates under physiological conditions, which are the optimal reaction conditions for most biological enzymes. Interestingly, GOx not only provides H2O2 for the second step reaction but also simultaneously leads to 7.4-fold enhancement of activity. We characterized the process of in situ generation of nanoceria at pH 7.0 and how proteins boost the activity by enhancing product desorption. In addition, the proposed one-pot bio-nanozyme cascade shows high stability and analytical performance for serum glucose with a detection limit of 5 μM.
研究领域:
生物化学
·
纳米医学
·
催化反应
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 9
·1410-1417
·
IF 7.571
Baofu Ma,Jianyu Han,Kun Zhang,Qike Jiang,Zhigang Sui,Zhixin Zhang,Baofeng Zhao,Zhen Liang,Lihua Zhang,Yukui Zhang
Nanozymes have great potential applications in tumor treatment due to their good stability, high biocompatibility, easy preparation and versatility. However, it remains a challenge to design highly active nanozymes with tumor cell targeting. Herein, three nanoceria structures (nanoceria-rod; nanoceria polyhedra, abbreviated as nanoceria-poly.; and nanoceria-cube) with different surface oxygen vacancy concentrations are designed. Among them, nanoceria-rod shows the highest enzyme activity and tumor cell toxicity because of its highest concentration of oxygen vacancies on the surface. Further study shows that nanoceria-rod can selectively enter tumor cells because nanoceria-rod with a suitable isoelectric point (IEP) remains positively charged in the acidic microenvironment of the tumor but negatively charged in the physiological microenvironment of normal cells. Nanoceria-rod distributes in lysosomes and phagosomes to produce reactive oxygen species (ROS) in tumor cells. Finally, the mitochondrial membrane potential (MMP) was reduced, which caused cell apoptosis. This study provides an interesting new tumor-targeting therapy method, which could also be extended to other drug nanocarriers and diagnostic imaging nanomaterials for tumors.
研究领域:
纳米技术
·
癌症治疗
·
材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Suhua Jiang,Fukai Zhu,Yang Lin,Liu Ran,Fulin Lin,Haina Tian,Ting Hu,Peiyuan Wang,Canzhong Lu
Owing to residual tumor tissues around the treatment margins, it is difficult to totally ablate large tumors, eliminate disseminated and metastatic nodules as well as prevent the tumor recurrence simultaneously by conventional photothermal therapy (PTT). In this work, PTT combined reactive oxygen species oxidative therapy (ROT) was conducted via mesoporous polydopamine nanosphere with nanoceria doping (MPDA-Ce). After surface anchoring with Arg-Gly-Asp (RGD) molecules, the dispersive stability and tumor targeting of this innovative nanoplatform were substantially boosted. In vitro studies revealed that the unique nanotherapeutics had satisfactory photothermal conversion efficiency (η = 51.41%), continuous nanoceria release in cytoplasm of tumor cells after acid microenvironment (pH = 5.5) induced biodegradation. Significantly, hyperthermia amplified the abundant reactive oxygen species generation under 808 nm laser irradiation that potent ROT was realized with only 27.1% cells alive. Strikingly, Indocyanine green (ICG) loaded MPDA-Ce with RGD modification shows robust tumor targeting capability in breast carcinoma bearing Balb/C mice under fluorescent imaging in the second near-infrared window. The combined treatments resulted in complete tumor cells eradication under 808 nm laser illumination both in vitro and in vivo . This work highlights the promising application of MPDA-Ce as a powerful nanoplatform for ROT and PTT combined treatment in clinical.
研究领域:
癌症治疗
·
纳米医学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）