ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 26
·6571-6580
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IF 4.157
Liu Donghao,Yang Ping,Wang Fangqi,Wang Cong,Chen Li,Ye Sijing,Dramou Pierre,Chen
Jianhua,He Hua
Nanozymes were the novel research field to replace natural enzymes because of stability and low cost. However, the research on nanozymes was mainly focused on peroxidase, and there was little research about nanozymes with oxidase-like activity, especially mimic oxidase of small molecules related to human physiology. High levels of uric acid (UA) in the body can cause hyperuricemia and gout. And natural uricase cured this disease because it could oxidize UA. The oxidase-like activity of mixed valence state metal organic frameworks with cerium (MVSM) had been studied, but MVSM was found to have uricase-like activity in this article. The catalytic process of UA with MVSM was studied by a variety of analytical methods, which was similar to the natural uricase except for further oxidation of H 2 O 2 . The catalytic activity constants of MVSM were acquired by the Michaelis-Menten equation. MVSM had a better ability to catalyze UA in in vivo and in vitro experiments. An enzyme-free analysis–based mimic uricase for UA was established. All the experimental results proved that MVSM had a good prospect to replace the natural uricase. Graphical abstract
A nanomaterial, mixed valence state Ce-MOF (MVSM), with uricase-like activity has been found in vivo and in vitro. This material has potential to be a fluorescent analysis for detecting uric acid without uricase.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Zhen Chen,Ziqi Zhang,Yixin Yu,Yu Guo,Jing Liu,Zhiling Zhu
A universal theory for predicting the catalytic activity of hydrolytic nanozymes has yet to be developed. Herein, by investigating the polarization and hydrolysis mechanisms of nanomaterials towards amide bonds, carbocation charge was identified as a key electronic descriptor for predicting catalytic activity in amide hydrolysis. Through machine learning correlation analysis and the Sure Independence Screening and Sparsifying Operator (SISSO) algorithm, this descriptor was interpreted to associate with the d-band center and Lewis acidity on the nanomaterial surface. On this basis, copper nanoparticles (Cu NPs) were discovered to exhibit significant hydrolytic activity. Further, peptidomic analysis and molecular dynamics simulations showed that Cu NPs demonstrated substrate selectivity. In the presence of water molecules, hydrophobic amino acid residues were driven towards the nanomaterial surface by hydrophobic groups of proteins, leading to the preferential hydrolysis of peptide bonds linked to these residues. This study provided a theoretic framework for predicting highly efficient hydrolytic nanozymes with broad potential applications.
研究领域:
生物化学
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材料科学
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计算化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2019
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IF 4.916
Shao-Bin He,Liu Yang,Xiu-Ling Lin,Li-Ming Chen,Hua-Ping Peng,Hao-Hua Deng,Xing-Hua Xia,Wei Chen
In this study, a colorimetric sensing assay of isoniazid based on excellent oxidase-like activity of heparin sodium stabilized platinum nanoparticles (HS-PtNPs) has been demonstrated. The newly prepared HS-PtNPs exhibit a great dispersion with an average size distribution of 4.8 ± 0.6 nm, and maintain more than 90% catalytic activity under strong acid and alkali or long-term storage conditions, indicating a robust nanomaterial with attractive potential. The HS-PtNPs show distinct oxidase-like activity with an ultrahigh affinity ( Km = 0.01012 mM) for 3, 3′, 5, 5′-tetramethylbenzidine (TMB). More significantly, we found that the pyridine ring of isoniazid has a strong reductive hydrazyl substitution, which can compete with TMB for the catalytic site of HS-PtNPs resulting in a colorless solution. Accordingly, a colorimetric sensing of isoniazid was fabricated. A linear relationship for isoniazid was achieved in 2.5 × 10 −6 to 2.5 × 10 −4 M (R 2 = 0.998) with a low limit of detection 1.7 × 10 −6 M (S/N = 3). Recovery experiments in drug tablets show that the standard recovery rates were 95%–103%. The quantitative detection data for isoniazid in drug tablets calculated respectively from the standard method and this method exhibited a high correlation coefficient (a slope of 0.9995), suggesting that high accuracy in isoniazid detection.
研究领域:
分析化学
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纳米医学
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药物检测
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2021
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IF 9.229
Yuting Zeng,Yan Li,Xiaofeng Tan,Jindi Gong,Ziyu Wang,Yuhao An,Zhenqiang Wang,He Li
Among plentiful porous nanomaterials, noble metal aerogels taken as nanozymes attract broad attention in sensing applications with their distinct enzyme mimic functions. In the catalytic field, the heteroatom doping strategy is a kind of way with great promise in improving the enzyme mimic activity of noble metal aerogels. In this experiment, we find a type of creative materials that were prepared by the fast and simple method. Due to the unique porous structure and synergetic effect from doped atoms, PdRu aerogels co-doped with boron and nitrogen (B, N-PdRu aerogels) were prepared using NH3BH3 as a reductant, which present improved peroxidase mimicking activity. With the existence of H2O2, the oxidation of 3,3′,5,5′-tetramethylbenzidine was catalyzed by B, N-PdRu aerogels fairly efficiently, whose solution would be a blue appearance at optimum absorption wavelength 652 nm. Thus, by the tandem reaction bound to the enzyme glucose oxidase, the B, N-PdRu aerogels can be used for the sensitive determination of glucose. The new method has a good linear detection effect for glucose in the range of 10 μM to 2 mM. The minimum limit of detection can reach as low as 6 μM. This work will contribute to research on the rational design of metal aerogels based on the heteroatomic doping strategy and enhance the corresponding performance for a variety of applications.
研究领域:
材料科学
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生物传感
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催化化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Yifei Li,Yucui Zhang,Huan Jiang,Mengyao Qi,Xia Zhang,Baowang Zhu,Lei Han
Although the fact that H 2 O 2 is employed in the medical, food, and chemical industries, trace detection of H 2 O 2 remains a difficult and significant problem. For the detection of H 2 O 2 residues, SERS assays based on enzyme inhibition techniques show promise. As a result, we csynthesized CeO 2 @nanogel/Au nanoparticles with dual enzyme-like capabilities to detect hydrogen peroxide in this study. CeO 2 nanozymes possess both peroxidase-like and oxidase-like activities which could oxidize colorless 3,3′,5,5′-tetramethylbenzidine to blue TMB oxide. Surprisingly, after the addition of nanogel, CeO 2 @nanogel were prepared and both of its dual enzymatic activities decreased, especially the oxidase-like enzymes almost disappeared. To better investigate the enzyme catalytic activity, Au NPs were introduced and CeO 2 @nanogel/Au nanozymes were self-assembly prepared. Based on the significantly enhancement of CeO 2 @nanogel/Au nanozymes peroxidase activity, we developed a Raman detection method for H 2 O 2 with TMB ox as the probe molecule. The Raman intensity showed good linearity for the concentration of H 2 O 2 in the range of 10 -9 M−10 −1 M with a correlation coefficient of R 2 = 0.9922 and a detection limit of 2.68 × 10 -12 M. Thus, the proposed SERS mode sensor exhibited good selectivity and interference resistance, providing a new avenue for environmental monitoring.
研究领域:
生物化学
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分析化学
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环境科学
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产品关联
DOI
高影响因子
APPLIED CLAY SCIENCE
·
2021
·
IF 5.467
Ping Chen,Hui Zhong,Xiao-rong Li,Meisheng Li,Shouyong Zhou
Nanomaterials with intrinsic enzyme-like activities have attracted extensive research interest as promising alternatives to natural biological enzymes due to their advantages of low cost and high stability. Many artificial enzyme mimics have been successfully applied in the fields of sensing, environmental treatment, and oncotherapy. Herein, novel [email protected] 3 O 4 nanocomposites ( [email protected] 3 O 4 NCs) were fabricated by modifying Pal with Co 3 O 4 nanoparticles through a facile hydrothermal method . The obtained [email protected] 3 O 4 nanocomposites as nanozymes displayed enhanced peroxidase-like activity, which can catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H 2 O 2 ) to obtain colored products. Kinetic assay indicated that the [email protected] 3 O 4 peroxidase mimics showed a higher affinity towards TMB than that of natural horseradish peroxidase (HRP). Moreover, a sensitive and selective sensor for colorimetric determination of H 2 O 2 and ascorbic acid (AA) was successfully constructed by using [email protected] 3 O 4 nanocomposites as peroxidase mimics. Furthermore, the proposed assay for sensing of AA in model samples was evaluated with satisfactory results, demonstrating the potential applications in complex biological systems.
研究领域:
生物传感
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纳米材料科学
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医学诊断
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
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2018
·
IF 0
Yanfang He,Xiangheng Niu,Longhua Li,Xin Li,Wenchi Zhang,Hongli Zhao,Minbo Lan,Jianming Pan,Xifeng Zhang
Nanomaterials with enzyme-like characteristics (nanozymes) are an emerging alternative of traditional bioenzymes that holds great promise in a variety of fields. The activity and efficiency of most current nanozymes, however, are lower than those of natural enzymes, which will inevitably hinder their wider applications. Thus, the development of new nanozymes with favorable catalytic properties is urgently desired. In this work, we reported a coral-structured bimetallic PdCu nanozyme that exhibited enhanced peroxidase activity and efficiency. The coral-like nanozyme fabricated by a microwave-assisted wet-chemical method was composed of assembled PdCu branches. In a comparison with pure Pd, the bimetallic PdCu had an enhanced ability to catalyze the reaction of colorless tetramethylbenzidine (TMB) to its blue oxide (TMBox) in the presence of hydrogen peroxide (H2O2). X-ray photoelectron spectroscopy and theoretical calculation indicated that the insertion of Cu atoms into the Pd lattice would increase the Pd0 proportion and decrease the adsorption difficulty of H2O2, both of which finally resulted in improvements in the catalytic activity and efficiency. It was further found that thiocyanate (SCN–) could inhibit the color reaction by decreasing the activity of PdCu selectively. On the basis of this principle, SCN– in the much wider range of 0.001–100 μM could be detected, along with a limit of detection down to 1 nM.
研究领域:
材料科学
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生物化学
·
分析化学
✓ 已验证
产品关联
DOI
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