APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 7
·2703-2714
·
IF 5.56
Geng Rui,Sun Yaning,Li Rui,Yang Jifei,Ma Hongfang,Qiao Zixuan,Lu Qingxia,Qiao Songlin,Zhang Gaiping
Abstract African swine fever virus (ASFV) causes a highly contagious and often lethal swine viral disease, and leads to tremendous economic losses to the swine industry. Unfortunately, there are no vaccines and effective antiviral agents available to prevent and control ASFV outbreaks. Therefore, it is necessary to develop simple and rapid strategies to monitor ASFV-infected pigs to restrain its spread. In the current study, ASFV capsid protein p72 was expressed along with its chaperone pB602L to form trimers in human embryonic kidney 293 (HEK293) cells. The p72 trimers were subsequently labeled with colloidal gold to develop a immunochromatographic strip. The strip showed high specificity to ASFV-positive serum and no cross-reactivity to other swine virus positive sera. Importantly, the strip showed a higher sensitivity of detecting ASFV antibodies in both positive standard serum and clinical serum samples than a commercial enzyme-linked immunosorbent assay (ELISA) kit. Taken together, these results demonstrate the strip as a reliable diagnostic tool against ASFV infection, which will be appropriate for application in prevention and control of ASFV.
Key points
• ASFV p72 trimers were successfully generated . • A colloidal gold strip was developed based on ASFV p72 trimers . • The strip is appropriate for detecting ASFV antibodies in the field .
研究领域:
兽医学
·
分子生物学
·
诊断技术
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Dan Qiao,Zhen Zhang,Lingshuang Wang,Wei Sheng,Qiliang Deng,Shuo Wang
Test strips as one of micro-devices are widely concerned in the fields of point-of-care detection and in-field diagnosing. For the purpose of on-site detection of tyramine, a carbon dots-molecularly imprinted fluorescent sensing test strip with high selectivity has been successfully obtained via a rapid in-situ preparation procedure (about 2.5 h). Here, the layer composed of carbon dots-molecularly imprinted polymer (CDs-MIP) with the function of the recognition and signal transduce has been formed on the surface of the filter paper strips via the sol-gel method. The developed test strip displayed a high specificity (Imprinting factor, IF = 4.96); and its fluorescence emission was quenched linearly with the increase of tyramine concentration from 0.5 to 10.0 mg L −1 . The limit of detection (LOD) is 0.059 mg L −1 (3 σ/s ). The application potential was verified by sensing of tyramine in rice vinegar and white rice vinegar with the recoveries of 90.9-104.4% (RSD < 5.8%). Many advantages including a rapid preparation and high selectivity make the fluorescence strip suitable for on-site detection of tyramine. In addition, the preparation procedure can also be easily transferred to the preparation of the fluorescence strip toward other targets.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2015
·Vol. 15
·期 2
·4291-4301
·
IF 2.245
Min Feng,Dezhao Kong,Wenbing Wang,Liqiang Liu,Shanshan Song,Chuanlai Xu
A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection ofPantoea stewartii subsp. stewartii(Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.Keywords:Pantoea stewartii subsp. stewartii(Pss);immunochromatographic test strip;monoclonal antibody
研究领域:
植物病理学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Jialin Hu,Xinxin Xu,Liguang Xu,Hua Kuang,Chuanlai Xu,Lingling Guo
Wheat gliadin is one of the most important causes of gluten sensitivity. This study aimed to develop a sandwich enzyme-linked immunosorbent assay (ELISA) and gold nanoparticle-based lateral flow (LFIA) strips to detect wheat allergen in food. Through cell screening, ten monoclonal antibodies (mAbs) against gliadin and after optimization were obtained, a pair of mAbs suitable for its detection (capture antibody: mAb 7; detection antibody : HRP-labeled mAb 6) were identified. Based on two of these antibodies, a sandwich ELISA with limit of detection (LOD) of 60 ng/mL in negative milk, and more importantly, negligible cross-reactivity to other allergens was developed. The average recoveries for gliadin in negative milk were 99.16%–100.07% using the sandwich ELISA. We also developed LFIA strips for the rapid detection of gliadin with visual limit of detection (vLOD) of 25 ng/mL and calculated LOD value of 6.56 ng/mL in negative milk. The test results of positive samples obtained from the LFIA strips were highly consistent with those of the sandwich ELISA. Thus, the developed LFIA strip is an effective and reliable tool for the rapid and on-site detection of wheat allergen in milk.
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Yanlei Wang,Feng Ding,Xiaoshuai Sun,Shijin Chen,Huarong Huang,Hong Chen
Hypochlorous acid (HClO) as well as its ionic form (ClO − ), representative of reactive oxygen species (ROS), are essential players in all sorts of biological processes. The abnormal level of each can lead to the onset of various diseases. Besides, Sodium hypochlorite , a commonly-used bleaching agent in our daily lives, could also result in breathing and skin problems when overexposed. Therefore, developing a molecular chemosensor for sensing HClO is of biological and environmental importance. Though many such chemosensors have been reported, new HClO chemosensors with different sensing performances may still come in handy in certain situations. In this work, we have developed a new coumarin-based chemosensor, CM-hbt , for realizing both ratiometric and colorimetric imaging detection of HClO in live cells. Notably, we further explored its application in sensing HClO in plant mung beans as well as fabricated an easy-to-use paper strip apparatus for facilitating its quick detection, which is seldomly seen in other HClO chemosensors. All the analysis results confirmed the high sensitivity and selectivity of this novel chemosensor. DFT calculations were used to decipher the underlying sensing mechanism of CM-hbt . Overall, this work presents a novel chemosensor, CM-hbt , as a colorimetric and ratiometric chemosensor for realizing imaging detection of HClO in a variety of different model systems, which highlights its broad spectrum of application potentials.
研究领域:
生物化学
·
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Mingya Wang,Shengling Li,Junli Shi,Yongzheng Liu,Duanlin Cao,Linxiu Zhao
A novel, highly sensitive “on-off-on” fluorescent probe ( E )- N ’-((1 H -imidazol-2-yl) methylene) -4-(5, 5-difluoro-1, 3, 7, 9-tetramethyl-5 H -4 λ 4 ,5 λ 4 -dipyrrolo [1, 2- c :2′, 1′- f ] [1,3,2] diazaborinin-10-yl) benzohydrazide ( BDB ) was developed in this study, and its structural and spectral properties were characterized by 13 C NMR, 1 H NMR, X-ray, ESI-MS, ultraviolet and fluorescence photometry. BDB could specifically and efficiently identify Cu 2+ among many other metal ions with a fluorescence quenching efficiency of 92.7% and a recognition limit of 0.65 μM in CH 3 CN/HEPES (7:3, v/v, pH = 7.23) buffer. The energy difference between BDB and BDB -Cu 2+ complex was calculated by DFT. The BDB -Cu 2+ complex could continuously identify GSH in the same system and show an obvious fluorescence enhancement response with a recovery efficiency of approximately 91% and a low recognition limit of 0.02 μM, which is significantly lower than the physiological concentration of GSH in living cells. The applicability of BDB is verified by the strip test. BDB can also be used to detect GSH in HeLa cells, which expand the application of fluorescent chemical sensors in biology.
研究领域:
分析化学
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生物化学
·
材料科学
·
生物成像
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale Research Letters
·
2016
·Vol. 11
·期 1
·1-8
·
IF 2.584
Yan Xinyu,Wang Kan,Lu Wenting,Qin Weijian,Cui Daxiang,He Jinghua
Carbohydrate antigen 72-4 (CA72-4) is an important biomarker associated closely with diagnosis and prognosis of early gastric cancer. How to realize quick, sensitive, specific, and quantitative detection of CA72-4 in clinical specimens has become a great requirement. Herein, we reported a CdSe/ZnS quantum dot-labeled lateral flow test strip combined with a charge-coupled device (CCD)-based reader was developed for rapid, sensitive, and quantitative detection of CA72-4. Two mouse monoclonal antibodies (mAbs) against CA72-4 were employed. One of them was coated as a test line, while another mAb was labeled with quantum dots and coated onto conjugate pad. The goat anti-mouse IgG was immobilized as a control line. After sample was added, a sandwich structure was formed with CA72-4 and these two mAbs. The fluorescent signal from quantum dots (QD)-labeled mAb in sandwich structure was related to the amount of detected CA72-4. A CCD-based reader was used to realize quantitative detection of CA72-4. Results showed that developed QD-labeled lateral flow strips to detect CA72-4 biomarker with the sensitivity of 2 IU/mL and 10 min detection time. One hundred sera samples from clinical patients with gastric cancer and healthy people were used to confirm specificity of this strip method; results showed that established strip method own 100 % reproducibility and 100 % specificity compared with Roche electrochemiluminescence assay results. In conclusion, CdSe/ZnS quantum dot-labeled lateral flow strips for detection of CA72-4 could realize rapid, sensitive, and specific detection of clinical samples and could own great potential in clinical translation in near future.
研究领域:
医学诊断
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生物化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yali Wang,Shuangbo Zhang,Jiang Bian,Joseph Brake,Chaoyang Luo,Chuanwen Sun,Xiaobin Wu
Mercury has caused severe harm to the environment and human health. A novel biological screen method was developed and identified a Hg 2+ chelator BDTH2. Both biological and chemical methods demonstrated BDTH2 displayed a high specificity and strong binding capacity for Hg 2+ . Toxicological experiments of BDTH2 demonstrated its good safety. To enhance the sensitivity, functional composite BDTH2-AuNPs were synthesized, and the system exhibited strong specificity to Hg 2+ and excellent anti-interference capabilities. The optimized colorimetric method and the paper test strip method showed a visual detection limit of 3 nM less than 30 s and 5 nM within 1 min respectively. This system was evaluated for detecting spiked Hg 2+ concentrations in environmental water and six agricultural food samples and showed high consistence with those of ICP-MS. Therefore, this study provides a novel, rapid, and economical method for on-site detecting of Hg 2+ in water and agricultural products.
研究领域:
环境科学
·
分析化学
·
毒理学
✓ 已验证
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Jiajia Sun,Liqiang Liu,Shanshan Song,Gang Cui,Hua Kuang
The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2022
·
IF 3.268
Kemeng Qin,Mingyue Ding,Chensi Zhang,Xijie Zhang,Yexuan Mao,Meng Dang,Zizhe Li,Youyi Wang,Shaohui Zhang,Yuhang Sun,Zhibin Zhang,Gaiming Zhao,Yu Li,Qian Li,Xiya Zhang
To improve the detection sensitivity of neomycin (NEO) in milk, we produced a sensitive monoclonal antibody (mAb) against NEO and developed a lateral flow immunoassay based on amorphous carbon nanoparticles (ACNPs-LFA). First, we conjugated NEO to carrier protein to prepare mAbs. We obtained six mAbs: mAb 1C6, mAb 1D3, mAb 2D3, mAb 4D5, mAb 5D1, and mAb 5H1. We characterised the mAbs by indirect competitive enzyme-linked immunosorbent assay and selected the most sensitive mAb based on the half-maximal inhibitory concentration (IC50). We selected mAb 4D5 (IC50 = 0.15 ng/mL) for the development of LFA. MAb 4D5 was labelled with ACNPs (ACNPs-mAb 4D5) by electrostatic absorption. Under optimised conditions, 5.4 μg mAb 4D5 coupling with 1 mL ACNPs, NEO-OVA at concentration of 80 μg/mL, 3 μL ACNPs-mAb 4D5 were used to develop LFA. The cut-off value was 8 ng/mL. Therefore, our developed ACNPs-LFA is suitable for on-site detection of NEO residues.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYST
·
2024
·
IF 3.6
Xuyang Ma,Liqiang Liu,Shanshan Song,Hua Kuang,Chuanlai Xu,Xinxin Xu
Glyphosate (GLY) is widely applied in agriculture and horticulture as a herbicide. The development of genetically modified plants has caused abuse of GLY, with excessive residues potentially causing harm to human health. Consequently, a novel method needs to be built to detect GLY in soybeans and corn. Computer simulation was used to design an excellent hapten which was used to produce an anti-GLY monoclonal antibody (mAb) with outstanding sensitivity and affinity, and its 50%-inhibitory concentration (IC50) was 128.59 ng mL−1. Afterwards, an immunochromatographic assay strip was developed based on the mAb. In soybeans and corn, the visual detection limits were 1 mg kg−1 and 0.2 mg kg−1, while the cut-off values were 50 mg kg−1 and 5 mg kg−1, respectively. The reliability of the strips was proved by the existing methods. Thus, a rapid method to detect GLY residues on-site in soybeans and corn was established.
研究领域:
农业科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Wen-Jie Zhu,Da-Qian Feng,Meng Chen,Zhi-Dong Chen,Rong Zhu,Hai-Lin Fang,Wei Wang
In this contribution, we first developed a semiquantitative method for the detection of glucose with self-calibration based on bienzyme colorimetry by using tree-shaped paper strip. The GOD/HRP bienzyme system was utilized to amplify the color signal in the aqueous phase. Moreover, we employed a paper as microfluidic media for running colorimetric assay, while tree-shaped paper strip was designed to ensure uniform microfluidic flow for multiple branches. Our proposed method gives direct outcomes which can be observed by the naked eye or recorded by a simple camera. The linear range is from 1.0 × 10 −3 to 11.0 × 10 −3 M, with a detection limit of 3 × 10 −4 M. Furthermore, the effect of detection condition has been investigated and discussed comprehensively. The result of determining glucose in human serum is consistent with that of detecting standard glucose solution by using our developed approach. A low-cost, simple, and rapid colorimetric method for the simultaneous detection of glucose with self-calibration on the tree-shaped paper has been proposed.
研究领域:
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）