Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 12
·1734-1743
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IF 4.776
Shuhao Wang,Fei Zan,Yu Ke,Gang Wu
Introducing or grafting molecules onto biomaterial surfaces to regulate cell destination via biophysical cues is one of the important steps for biomaterial design in tissue engineering. Understanding how cells feel the substrate makes it easier to learn the mechanism behind cell–material interaction. In this study, on a glass substrate, we constructed poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths via a surface-induced atom transfer radical polymerization (SI-ATRP) method. FTIR-ATR and XPS tests of the formed polymer brushes indicate that these brushes have characteristic chemical structures of PHEMA; the polymer brush length revealed by the AFM tests increases linearly with reaction time. Cell lines of BMSCs, ATDC5, and human chondrocytes (HC) were cultured on these substrates to evaluate proliferation, adhesion, and differentiation. Our results demonstrated that the cells cultured on the substrates with short PHEMA brushes developed a spread morphology and organized actin fibers as compared to the cells cultured on those with long brushes. Different cell lines showed different responses depending on the PHEMA brush length. Cells cultured on long PHEMA brushes displayed a more rounded shape, higher gene expression of FAK and integrin, and lower gene expression of NCAM and N-cadherin as compared to those, especially ATDC5 cells, cultured on short PHEMA brushes. On PHEMA brushes with a long length, the cell lines express higher cartilage-specific genes including Sox9 and Col2 and GAG in ECM. The results suggest that polymer brushes having different lengths may interfere with the behavior of the cells cultured on them.
研究领域:
生物材料学
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组织工程学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Ke Deng,Yafei Wang,Jinsheng Li,Xianli Fan,Lei Wang,Javad Harati,Pengfei Chen,Lijun Wang,Zheng Zhou,Xianggui Chen,Jua Kim,Haobo Pan,Tailin Guo
Surface patterns are widespread in many organisms, providing the abilities of camouflage, recognition, sexual selection, and survival in creatures. Inspired by these features, structural color stripes over large and complex surfaces were presented based on a meniscus rapidly receding assembly process triggered by lowered pressure and heat. The pattern method allows building blocks to rapidly self-organize into structural color stripes with adjustable morphology and color within several minutes on 2D or complex 3D surfaces. We demonstrate that the structural color striped pattern can be used in anti-counterfeiting, mimicking structural color organisms, and manipulation of stem cell behaviors at multiple levels. This lowered pressure and heat triggered pattern method for engineering scalable structural color stripes provides a powerful tool which cannot be achieved by conventional methods and will promote the wide application of structural color pattern in sensing, artificial robots, anti-counterfeiting, and so on.
研究领域:
材料科学
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生物仿生学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Xueshi Luo,Shuyun Zhang,Binghong Luo,Hong Li
Nanostructure provides a closer structural support approximation to native bone architecture for cells and further regulates cell's behavior, resulting in the formation of functional tissues. In this work, three engineering collagen templates with oriented fiber architectures were fabricated via electrospinning (Es), plastic compression and tensile (PCT), and dynamic shear stress (SS) methods. Under the observation of POM, SEM, AFM and TEM, the PCT-template and SS-template are packed with well-oriented nanofibers with the native collagen architecture of 67 nm D-periodicity, and the mechanical properties conferred to the templates are better than that of the Es-template. When mentioning the cell's behavior, MC3T3-E1 adhered to grow along the alignment of collagen fiber orientation when cultured on the PCT-template and SS-template. The SS-template with nano- and micro-ordered architecture guided cells to stretch their plasma along with the orientation of collagen fiber, produce more aligned Type I collagen fibers and promote significantly higher osteogenic differentiation of MC3T3-E1 than the PCT-template and Es-template. Overall, it is strongly argued the feasibility of hierarchical collagen fiber architectures for bone tissue regeneration.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2017
·
IF 2.986
Shenglei Feng,Fupo He,Jiandong Ye
In this study, for the first time honeycomb β-tricalcium phosphate (β-TCP) scaffolds were fabricated through an extrusion technique. The physicochemical properties and cell behaviors of the honeycomb β-TCP scaffolds were investigated. The results showed that scaffolds were characterized by ordered channel-like macropores and unidirectional interconnection. The pore structure and mechanical strength could be tailored by changing the parameters of extrusion molds. The pore size of scaffolds was in the range of 400–800 µm approximately, while their compressive strength parallel to the pore direction and porosity ranged from 14 to 20 MPa and 60–70%, respectively. The in vitro cell behavior demonstrated that cells could well attach on the surfaces and grow into the inner channel-like pores of the scaffolds; the scaffolds with higher porosity showed better cell proliferation but poorer cell differentiation. The honeycomb scaffolds fabricated by extrusion technique are potential candidate for bone tissue engineering.
研究领域:
生物材料学
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组织工程学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Jiao Yang Lu,Fu Rui Zhang,Xue Zhi Ding,Li Qiu Xia,Wei Tao Huang
Extracellular matrix (ECM) with various micro/nano features is an important chemical and physical clue to control cell behaviors. Fish scale surface has rich structures and similar components to ECM, but there are no reports on directly using the natural surface of fish scales to guide cell behavior. Herein, natural interface of waste fish scales can be directly, simply, green, and low-costly used to regulate cell behavior. A series of characterization showed that the natural surface of fish scales is rich in micro/nano structures (groove, short ridge, fibers), contain collagen and hydroxyapatite, has suitable wettability and no cytotoxicity. These natural structures on waste fish scales have adjustable cell guidance abilities, which are consistent with the anisotropy levels of these structures (ordered fibers > grooves > damaged fibers > short ridges). Moreover, cell contact interaction with micro/nano topographies on the fish scale surface is dynamically adjustable with growth time, and also increased the chemoresistance of cancer cells to drug (irinotecan and cisplatin). Due to its advantages of wide material sources, waste recycling, patterning-free, and pretreatment-free, directly using biowastes (fish scales) not only provides a new opportunity for the development of in vitro anisotropic culture platform, but also helps to deepen the understanding and application of natural materials.
研究领域:
生物材料学
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细胞生物学
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再生医学
✓ 已验证
产品关联
DOI
高影响因子
DENTAL MATERIALS JOURNAL
·
2025
·
IF 1.9
Ting YANG,Wenjie HU,Kwok-Hung CHUNG,Fuhua YANG,Hua YANG
Sandblasted with large grits and acid-etched (SLA) surface topography has been a common method of implant surface modification to promote bone-implant integration in titanium-based dental implant manufacturing. However, the acid etching protocols used are various. The aim of this investigation was to validate the effect of different acid-etched treatments of sandblasted titanium surfaces on enhancing osteogenic potential. Five different titanium surface groups were prepared and tested including: i) smooth (Control), ii) sandblasted (SL), iii) sandblasted and acid-etched with H2SO4/HCl mixed solution (SLA/SOCl), iv) sandblasted and acid-etched with H2SO4 solution (SLA/SO), and v) sandblasted and acid-etched with HF solution (SLA/HF). The surface characterization, protein adsorption, and cell behavior were examined to determine the osteogenic potential. Compared with the control group, other surface treated groups showed rougher surface, better wettability, more protein absorption, and cell viability, especially in the SLA/SOCl surface group. The results indicated that SLA surfaces prepared with H2SO4/HCl mixed acid solution provided significant potential to promote osteogenesis.
研究领域:
生物材料学
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口腔医学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 29
·4703-4710
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IF 0
Hui Yang,Huijun Zeng,Lijing Hao,Naru Zhao,Chang Du,Hua Liao,Yingjun Wang
Understanding the shape effect of hydroxyapatite (HAp) microparticles on cellular behavior is important for enabling new kinds of biological and biomedical applications. However, it is still a challenge to prepare HAp microparticles with different shapes but similar physicochemical properties, and then to investigate their relationships with cellular behavior. Herein, we developed a novel, facile route to regulate the morphology of HAp microparticles, and investigated the interaction between the particles and bone marrow mesenchymal stem cells (BMSCs). Our results revealed that the shape of HAp has a strong influence on cellular behavior, and that the sphere-like particles performed better than the rod-like particles. These findings highlight the importance of the shape characteristics of HAp microparticles, and may provide new insights for the utility of HAp-based materials.
研究领域:
生物材料
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细胞生物学
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组织工程
✓ 已验证
DOI
PubMed
JOURNAL OF SURGICAL RESEARCH
·
2009
·
IF 1.875
Ole Goertz,Stefan Baerreiter,Andrej Ring,Birger Jettkant,Tobias Hirsch,Adrien Daigeler,Hans U. Steinau,Stefan Langer
Background The breakdown of skin microcirculation and the leukocyte–endothelium interaction are assumed to play a key role in the pathophysiology of frostbite injuries. However, little is known as yet. The aim was to develop an in vivo frostbite model to monitor microcirculatory changes and angiogenesis after frostbite injury. Materials and Methods Deep partial thickness frostbite injuries were inflicted with a no-touch-technique to the ears of hairless mice ( n = 9). To this end, a gas jet of nitrogen vapor (T = –195,8 ± 2.7°C) was delivered onto an area of 1.9 mm 2 for 1,5 s. Intravital fluorescent microscopy in combination with FITC-dextran and Rhodamin 6 G as fluorescent dyes was used to assess microcirculatory changes, leukocyte behavior, and angiogenesis during the 14 d of wound healing. Results The area of no perfusion decreased significantly over the observed period, and perfusion was almost completely restored due to angiogenesis by d 14 (day 1: 1.89 [mm 2 ] ± 0.44SEM, d 14: 0.02 ± 0.01). No post-traumatic extension of the trauma could be observed. Edema formation increased significantly up to d 7. The number of adherent leukocytes showed a significant increase during the first 7 d. Functional vessel density showed a significant post-frostbite decrease to 60% of the baseline value. Conclusions This novel frostbite model provides a simple and nonetheless highly effective technique of creating locally limited reproducible frostbite injuries using a no touch technique. Tissue damage can be fully attributed to the thermal trauma, and the model allows repetitive intravital fluorescent microscopy of the microcirculation, leukocyte–endothelium interaction, and angiogenesis.
研究领域:
医学
·
生物学
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Yanli Liu,Jiazhu Xu,Yan Zhou,Zhaoyang Ye,Wen-Song Tan
Cellular behaviors can be affected by both surface chemistry and topography of biomaterials substrates. The object of the present study was to investigate how pore structure and bioactive molecules regulate the adhesion and proliferation of rabbit bone marrow-derived mesenchymal stem cells (rMSCs) in synergy. Poly(ε-caprolactone) (PCL) films with a honeycomb-like porous structure were fabricated via a breath-figure method, and then further coated with bioactive molecules including four combinations of polyelectrolytes (GEL/CS, GEL/HA, CHI/CS and CHI/HA) via a layer-by-layer self-assembly (LBL) process. rMSCs were seeded on these films to evaluate both adhesion and proliferation. It was shown that a uniform honeycomb-like porous structure with a pore size of 18.87 ± 3.25 μm was obtained in the films. Bioactive molecules were proven to be successfully deposited on the films, the amount of which increased with the number of assembled layer. Both surface pore structure and assembled molecules play significant roles on the adhesion and proliferation of rMSCs. Notably, the spatial distribution of cells (either entrapped in pores or spreading over pores) on porous films was also dictated by the identity of the assembled molecules. These findings might be fundamental to design bioactive scaffolds for tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Zhibo Yang,Xiaopeng Yu,Jiajie Chen,Wenping Ma,Jianxin Hao,Chengtie Wu
The ordered topological micro/nanostructures of scaffolds play a pivotal role in regulating bone development, remodeling, and regeneration. Nevertheless, achieving the integration of ordered micro/nanostructures into 3D scaffolds remains a formidable challenge. In this context, a brushing-assembly strategy is developed to construct 3D bioactive scaffolds with highly ordered micro/nanostructures. Such an engineered scaffold exhibits a positive regulatory effect on the behavior and fate of bone resident cells, such as mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs), through mechanical stimulation provided by the ordered micro/nanostructures, while also allowing for the precise spatial distribution of multiple cell types through assembly. In vivo experiments demonstrate that scaffolds with ordered nanostructures possess the potential to accelerate vascularized bone regeneration. Overall, this work proposed a universal strategy for the fabrication of bioactive scaffolds with ordered topological micro/nanostructures, bridging the gap between 3D scaffolds and ordered surface microstructures for tissue engineering.
研究领域:
组织工程
·
再生医学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Chen Shuo,Wang Xinqing,Zhang Dong,Huang Zhenhua,Xie Yina,Chen Fangping,Liu Changsheng
Poly (l-lactic acid) (PLLA) is a biocompatible, biodegradable material with piezoelectric properties, making it a promising candidate for providing self-powered stimulation to accelerate tissue repair. Repairs to various tissues, such as bone, cartilage, and nerve, necessitate distinct piezoelectric characteristics even from the same material. However, the extensive utilization of PLLA piezoelectric scaffolds in various tissue is hindered by their low and single piezoelectric constants. In this study, PLLA nanofiber membranes with enhanced and adjustable piezoelectric constants (d33) were fabricated through oriented electrospinning. By carefully controlling the parameters of the spinning solution, a steady increase in d33 values from 0 to 30 pC/N was achieved. This advancement allows tailoring of PLLA nanofiber membranes to meet various piezoelectric requirements of different tissues. As an example of tailoring the optimal piezoelectric constants, we developed PLLA-2-0, PLLA-2-10, PLLA-2-15 and PLLA-2-20 nanofiber membranes with d33 values of 0, 5, 10 and 15 pC/N, respectively. The impact of varying piezoelectric constants of PLLA nanofiber membranes on cellular behavior and repair efficacy were validated through in vitro cellular experiments and in vivo mandibular critical defect repair. The results indicated that PLLA-2-20 demonstrated superior cell proliferation rate up to 130% and an osteogenic differentiation level approximately twice of the control. In addition, PLLA-2-20 significantly promoted cell adhesion and migration, and the cell aspect ratio was about 5 times higher than that of the control group. In vivo, PLLA-2-20 optimal restorative effects on rat mandibles via endogenous mechanical force-mediated piezoelectric stimulation, leading to complete histological restoration within 8 weeks. These findings highlight the potential of the PLLA membranes with high and adjustable d33 by a straightforward process. This study provides a novel approach for the development of highly electroactive membranes tailored to specific tissue repair needs.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Jiao Yang Lu,Qiu Yan Zhu,Xin Xing Zhang,Fu Rui Zhang,Wei Tao Huang,Xue Zhi Ding,Li Qiu Xia,Hong Qun Luo,Nian Bing Li
Due to rapid change in information technology, many consumer electronics become electronic waste which is the fastest-growing pollution problems worldwide. In fact, many discarded electronics with prefabricated micro/nanostructures may provide a good basis to fulfill special needs of other fields, such as tissue engineering, biosensors , and energy. Herein, to take waste optical discs as an example, we demonstrate that discarded electronics can be directly repurposed as highly anisotropic platforms for in vitro investigation of cell behaviors, such as cell adhesion, cell alignment, and cell-cell interactions. The PC12 cells cultured on biocompatible DVD polycarbonate layers with flat and grooved morphology show a distinct cell morphology, indicating the topographical cue of nanogrooves plays a key role in guidance of neurites growth. By further monitoring cell morphology and alignment of PC12 cells cultured on the DVD nanogrooves at different differentiation times, we find that cell contact interaction with nanotopographies is dynamically adjustable with differentiation time from initial disorder to final order. This study adds a new dimension to not only solving the problems of supply of materials and fabrication of nanopatterns in neural tissue engineering, but may also offering a new promising way of waste minimization or reuse for environmental protection.
研究领域:
生物医学工程
·
组织工程
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）