BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Chunyan Li,Shuting Wang,Xiaopeng Du,Xiaosong Cheng,Meng Fu,Ning Hou,Dapeng Li
In this study, three bacteria with high Fe- and Mn-oxidizing capabilities were isolated from groundwater well sludge and identified as Acinetobacter sp., Bacillus megaterium and Sphingobacterium sp. The maximum removal ratios of Fe and Mn (99.75% and 96.69%) were obtained by an optimal combination of the bacteria at a temperature of 20.15 °C, pH 7.09 and an inoculum size of 2.08%. Four lab-scale biofilters were tested in parallel for the removal of iron and manganese ions from groundwater. The results indicated that the Fe/Mn removal ratios of biofilter R4, which was inoculated with iron- and manganese-oxidizing bacteria and a biofilm-forming bacterium, were approximately 95% for each metal during continuous operation and were better than the other biofilters. This study demonstrated that the biofilm-forming bacterium could promote the immobilization of the iron- and manganese-oxidizing bacteria on the biofilters and enhance the removal efficiency of iron and manganese ions from groundwater.
研究领域:
微生物学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Ziqi Liu,Xingjia Yin,Naidong Xiao,Xiaoqiong Wan,Jinlong Hu,Yumei Hua,Guanglong Liu,Jianwei Zhao
Submerged macrophytes have important impacts on the denitrification and anaerobic ammonia-oxidizing (anammox) processes. Leaf damage in these plants probably changes the rhizosphere environment, affecting organic acid release and denitrifying bacteria . However, there is a lack of comprehensive understanding of the specific changes. This study investigated these changes in the rhizosphere of Potamogeton crispus with four degrees of leaf excision. When 0 %, 30 %, 50 % and 70 % of leaves were excised, the concentrations of total organic acid were 31.45, 32.67, 38.26, and 35.16 mg/L, respectively. The abundances of nirS -type denitrifying bacteria were 2.10 × 10 10 , 1.59 × 10 10 , 2.54 × 10 10 , and 4.67 × 10 10 copies/g dry sediment, respectively. The abundances of anammox bacteria were 7.58 × 10 9 , 4.59 × 10 9 , 3.81 × 10 9 , and 3.90 × 10 9 copies/g dry sediment, respectively. The concentration of total organic acids and the abundance of two denitrification microorganisms in the rhizosphere zone were higher than those in the root zone and non-rhizosphere zone. With increasing leaf damage, the number of OTUs in the Pseudomonas genus of nirS- type denitrifying bacteria first increased and then decreased, while that of the Thauera genus was relatively stable . The overall increase in the OTU number of anammox bacteria indicated that leaf damage promotes root exudates release, thereby leading to an increase in their diversity. The co-occurrence network revealed that the two denitrification microorganisms had about 60.52 % positive connections in rhizosphere while 64.73 % negative connections in non-rhizosphere. The abundance and community composition of both denitrification microorganisms were positively correlated with the concentrations of various substances such as oxalic acid , succinic acid , total organic acids and NO 2 - -N. These findings demonstrate that submerged plant damage has significantly impacts on the structure of denitrification microbial community in the rhizosphere, which may alter the nitrogen cycling process in the deposit sediment. Synopsis This study reveals leaf damage of macrophyte changed the rhizosphere denitrification microbial community , which is helpful to further understand the process of nitrogen cycle in water.
研究领域:
生态学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Yuhang Yang,Bing Li,Mingxi Li,Zhiyi Deng,Zuoyi Chen,Jinhua Wu
Zero-valent iron (Fe 0 ) usually suffers from organic acid complexation and ferrochrome layer passivation in Cr(VI) removal from bioleached wastewater of Cr slag. In this work, a synergetic system combined Fe 0 and mixed hetero/autotrophic bacteria was established to reduce and stabilize Cr(VI) from bioleached wastewater. Due to bacterial consumption of organic acid and hydrogen, severe iron corrosion and structured-Fe(II) mineral generation (e.g., magnetite and green rust) occurred on biotic Fe 0 surface in terms of solid-phase characterization, which was crucial for Cr(VI) adsorption and reduction. Therefore, compared with the abiotic Fe 0 system, this integrated system exhibited a 6.1-fold increase in Cr(VI) removal, with heterotrophic reduction contributing 3.4-fold and abiotic part promoted by hydrogen-autotrophic bacteria enhancing 2.7-fold. After reaction, the Cr valence distribution and X-ray photoelectron spectroscopy indicated that most Cr(VI) was converted into immobilized products such as Fe x Cr 1-x (OH) 3 , Cr 2 O 3 , and FeCr 2 O 4 by biotic Fe 0 . Reoxidation experiment revealed that these products exhibited superior stability to the immobilized products generated by Fe 0 or bacteria. Additionally, organic acid concentration and Fe 0 dosage showed significantly positive correlation with Cr(VI) removal within the range of biological adaptation, which emphasized that heterotrophic and autotrophic bacteria acted essential roles in Cr(VI) removal. This work highlighted the enhanced effect of heterotrophic and autotrophic activities on Cr(VI) reduction and stabilization by Fe 0 and offered a promising approach for bioleached wastewater treatment .
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2018
·Vol. 303
·期 5
·1700590
·
IF 2.69
Qian Wang,Yunbo Feng,Min He,Yanping Huang,Weifeng Zhao,Changsheng Zhao
Herein, ene-functionalized dopamine is first coated onto a polyether sulfone membrane surface to introduce double bonds as anchoring sites for hydrogel film. Then, a hydrogel film is synthesized and simultaneously attached onto the membrane via photoinduced crosslinking copolymerization of N -isopropylacrylamide (NIPAAm) and methacryloxyethyltrimethyl ammonium chloride. The thickness of the hydrogel film can be well-controlled ranging from the nanometer to micrometer scales, which can retain the permeability of the membrane. Water contact angle and swelling tests demonstrate that the modified membrane shows thermoresponsive surface wettability. The quaternary ammonium salts in the hydrogel film can kill the attached bacteria efficiently and the dead bacteria can be detached by reducing the temperature below the lower critical solution temperature of poly(NIPAAm). The modified membrane also shows improved hemocompatibility, confirmed by prolonged clotting time and low hemolysis ratio. Hence, the designed thermoresponsive antibacterial hydrogel film with enhanced hemocompatibility has great potential to be applied in biomedical areas.
研究领域:
生物医学工程
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材料科学
✓ 已验证
DOI
高影响因子
BMC MICROBIOLOGY
·
2025
·Vol. 25
·期 1
·1-12
·
IF 4.2
Yang Manli,Zuo Shanningmei,Liu Ahui,Zhong Nana,Lu Xueping,Liu Xun,Jiang Xiasen,Hu Tao,Liu Yuntong,Ren Xiaogang,Zhou Kang,Wu Chuansheng
Background Bisphenol A (BPA), a widespread environmental pollutant, has been extensively studied for its effects on bacteria and plant, but its impact on rhizosphere bacterial communities and plant root traits is less understood. At the same time, the role of bacteria in helping plants resist adversity is widely recognized, but the relationship between BPA-induced with rhizosphere bacterial changes and root development is still unclear. Therefore, this study investigated the effects of varying BPA concentrations (1.5, 17.2, and 50 mg/L) on soybean root traits and rhizosphere bacterial communities, as well as the relationship between them. Result The results revealed that BPA exposure significantly altered root traits, with root length, surface area, volume, and tip numbers being suppressed at 50 mg/L, while lower concentrations (1.5 and 17.2 mg/L) promoted root elongation and thickening. Bacterial community composition shifted notably, with Bacillota increasing and Pseudomonadota decreasing in relative abundance across all BPA treatments. Alpha diversity, measured by richness and Shannon_e indices, increased slightly at lower BPA concentrations, while beta diversity (Bray_Curtis and UniFrac) analysis showed significant differences, particularly at 50 mg/L. Community assembly processes (βNRI and βNTI) were dominated by deterministic mechanisms at lower BPA concentrations but shifted toward stochastic processes at 50 mg/L. Correlation analysis revealed significant relationships between bacterial community dynamics and root traits (Principal component PC1 and PC2), with alpha diversity indices influencing root traits represented by PC2 and beta diversity indices showing a negative correlation with PC1. Conclusions BPA exposure not only alters root morphology and bacterial community structure but also highlights the intricate interplay between rhizosphere bacteria and plant roots under BPA stress. This study contributes to the theoretical understanding of plant–microbe interactions in contaminated environments and may inform future research on microbial involvement in plant stress responses.
研究领域:
植物科学
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环境毒理学
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微生物生态学
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农业污染
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Shan Ye,Lei Jiang,Jimin Wu,Chen Su,Chaobo Huang,Xiufeng Liu,Wei Shao
In this study, we report the design and fabrication of a novel biocompatible sponge with excellent antibacterial property, making it a promising material for wound dressings. The sponge is formed by grafting amoxicillin onto regenerated bacterial cellulose (RBC). It was observed that the grafted RBC could enhance the antibacterial activity against fungus, Gram-negative, and Gram-positive bacteria. The morphology of strains treated with the grafted RBC and fluorescent stain results further demonstrated the antibacterial ability of the fabricated sponge. Moreover, a cytocompatibility test evaluated in vitro and in vivo illustrates the nontoxicity of the prepared sponge. More importantly, the wound infection model reveals that this sponge can accelerate the wound healing in vivo. This work indicates the novel sponge has the huge potential in wound dressing application for clinical use.
研究领域:
生物材料学
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医学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2017
·
IF 4.747
Huiqiong Yan,Xiuqiong Chen,Huangwang Song,Jiacheng Li,Yuhong Feng,Zaifeng Shi,Xianghui Wang,Qiang Lin
Although bacterial cellulose (BC) and bacterial cellulose nanocrystals (BCNs) have considered to be edible, non-toxic, biocompatible and biodegradable, there is still the dearth of reports of BC and BCNs as the emulsifiers of Pickering emulsions with respect to the applications in foods , cosmetics and medicines. In this work, BC with ultrafine network architecture and 8–40 nm crystalline microfibrils in diameter was produced by Acetobacter xylinum , and then it was hydrolyzed by sulfuric acid followed by the oxidation of hydrogen peroxide to prepare BCNs. The physicochemical properties of BC and BCNs were contrastively evaluated by FT-IR, XRD, TGA and dynamic light scattering (DLS). The emulsifying performances of BC and BCNs in the stabilization of olive oil Pickering emulsion were further evaluated by optical microscope (OM) and fluorescent microscope (FM). Experimental results showed that the sulfuric acid hydrolysis of BC induced the removal of the amorphous components and the cleavage of the crystalline microfibrils, making BCNs possess the initiating decomposition temperature of 350 °C, the crystallinity index (CrI) of 89.6%, average size of 259.6 nm with PDI of 0.26 and zeta potential at about −34.8 mV, which exhibited high thermal stability and good emulsifying performance in comparison with BC. Furthermore, the effects of pH and ionic strength on emulsion stability were also investigated. In comparison to BC, BCNs were more sensitive in response to the change of pH and ionic strength.
研究领域:
食品科学
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2025
·Vol. 15
·期 11
·778
·
IF 4.3
Weimin Ning,Mingxuan Li,Lei Jiang,Mei Yang,Maoyan Liu,Yong Liu
Bacterial leaf blight (BLB) is a destructive disease caused byXanthomonas oryzae pv. oryzae(Xoo). It has been proven that BLB adversely influences the growth and production of rice, resulting in substantial losses in yield. Nanoparticle–antimicrobial compounds possess excellent physicochemical properties, which have generated groundbreaking applications in protecting rice against BLB attacks. However, there is less research focused on the interaction between nanoparticles and the microbiome of BLB rice leaves, particularly the structure and function of endophytic bacteria, which are essential to plant health and pathogenesis. Therefore, the study explored how Cu-Ag nanoparticles influenced the endophytic bacteria’s composition and functions in healthy and BLB rice leaves. The data demonstrated that the relative abundance of beneficial bacteria,Burkholderiales,Micrococcales, andRhizobiales, increased after the introduction of Cu-Ag nanoparticles on the leaves of BLB rice. The examination of PAL activity demonstrated that nanoparticles limited the spread ofXooin rice leaves. Furthermore, endophytic community functional prediction demonstrated that nanoparticles may regulate the physiological process associated with potential stress resistance and growth-promoting function in the endophytic communities. This investigation may enhance the understanding of interactions between nanoparticles and the composition of rice endophytic microbiome, which can contribute to the exploration and application of nanomaterials in crop pathogen management.
研究领域:
植物病理学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Hairong Zhang,Haijun Guo,Bo Wang,Silan Shi,Lian Xiong,Xinde Chen
In this work, bacterial cellulose (BC) was activated by ethylenediamine (EDA) and then dissolved in lithium chloride/N,N-dimethylacetamide (LiCl/DMAc) aqueous solutions. The resulting transparent solution was cast on a glass plate to prepare regenerated BC. Then cationic BC was prepared homogeneously by the reaction between regenerated BC and 3-chloro-2-hydroxypropyl-trimethyl ammonium chloride (CHPTAC) in a NaOH/urea aqueous solution. Structure and properties of the BC and its products were characterized by different techniques such as X-ray diffraction (XRD), Fourier transform spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermo-gravimetric analysis (TGA). The results showed that there was no significant difference between the structures of BC, activated BC and regenerated BC. The effects of different temperature and molar ratio of CHPTAC to anhydroglucose unit (AGU) on the degree of substitution (DS) value were examined. The DS values of cationic BC ranged between 0.21 and 0.51.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yulong Lan,Yao Wang,Xiaoliang Qi,Erya Cai,Yajing Xiang,XinXin Ge,Hangbin Xu,Xiaojing Chen,Ying Li,Yizuo Shi,Jianliang Shen,Zhiyong Liao
Management of diabetic wounds becomes increasingly challenging as bacterial infections intensify the inflammation. Employing polysaccharide hydrogels with inherent antibacterial qualities can significantly reduce the need for antibiotics to manage infections in diabetic wounds. The typical approach to achieving antibacterial outcomes with hydrogels relies on the penetration of bacteria into their porous architecture. Such penetration not only takes time but can also prolong inflammation, thus impeding the healing of wounds. Hence, the quick capture and eradication of bacteria are essential for optimizing the hydrogel's antibacterial performance. Herein, we introduce a multifunctional polysaccharide hydrogel dressing—designated as HAQ—created for managing bacterial infections in diabetic wounds. This dressing is based on hyaluronic acid, which is modified with methacrylic anhydride, and special functional groups are added to the modified hyaluronic acid matrix: phenylboronic acid for capturing bacteria and quaternary ammonium chitosan for bacterial destruction. As expected, the HAQ system exhibits robust antibacterial effectiveness against both methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa in vitro and in vivo. Consequently, HAQ stands as a promising hydrogel dressing with intrinsic antibacterial capabilities and offers significant potential for managing diabetic wounds infected by drug-resistant bacteria.
研究领域:
生物材料学
·
医学
·
感染控制
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Liuqian Yang,Yaoyu Luo,Yikai Zhou,Chuixiu Huang,Xiantao Shen
Antibiotic resistance has been a worsening global concern and selective elimination of antibiotic-resistant bacteria (ARB) while retaining the co-existed beneficial bacteria has been essential in environmental protection, which having attracted considerable interest. In this work, by integrating the whole cell imprinting and epitope imprinting strategy, magnetic bacterial imprinted polymers (BIPs) towards ARB were synthesized with interfacial biomimetic mineralization followed by a screening process. The binding data showed that the BIPs owned highly specific affinity towards the target bacteria. Taking advantage of this specific binding ability of BIPs, a two-step selective antimicrobial approach was developed. Remarkably, the BIP nanoantibiotics (nAbts) could efficiently destroy ARB without harming the beneficial bacteria. In comparison with the non-bacterial imprinted polymers, the biocompatible BIP nAbts showed a 12.5-fold increase in the survival percentage for the beneficial bacteria in wastewater. To the best of our knowledge, this is the first time that bacterial imprinting via interfacial biomimetic mineralization was developed, and also the first report of killing ARB without harming the beneficial bacteria in wastewater. We believe that this strategy provides a new insight into the design of novel affinity materials for the selective elimination of ARB in biological treatment for environmental protection.
研究领域:
环境科学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG DEVELOPMENT RESEARCH
·
2019
·Vol. 80
·期 6
·750-757
·
IF 1.742
Chang Zheng,Wen Hou,Jun Liu,Xiao-Fang Xu,Jing Lin,Ping-Hua Sun,Wei-Min Chen
The prevalence of multidrug resistance among clinically significant bacterial pathogens underlines a critical need for the development of new classes of antibacterial agents with novel structural scaffolds. Cajaninstilbene acid (CSA), which is isolated from pigeonpea leaves, has shown potent antibacterial activity. In this study, a series of 2-hydroxyl-4-methoxyl-3-(3-methylbut-2-en-1-yl)-6-(4-phenylbenzoylamino)benzoic acid derivatives derived from CSA were designed and synthesized, and their antibacterial activities were evaluated. Several synthesized compounds exhibit better antibacterial activity than CSA against Staphylococcus aureus , Staphylococcus epidermidis , and two strains of methicillin-resistant S. aureus . Meanwhile, the results of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assays illustrate the good selectivity between bacteria and normal cells of the most active compounds 6u and 6v . Furthermore, well combinations with bacterial RNA polymerase of 6u arising from docking study imply the possible mechanism of antibacterial activity of these synthetic compounds.
研究领域:
药物化学
·
微生物学
·
合成化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）