African Journal of Pharmacy and Pharmacology
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2017
·Vol. 12
·期 47
·582-590
·
IF 0
Raphael N. Alolga,Enos Mais,Vitus Onoja
The quality of botanicals has been reported to be influenced by their places of cultivation. Although, ginger remains a common health remedy both in Ghana and China, no comparative study exists to the best of the authors’ knowledge, on the anti-inflammatory properties of ginger from these two countries. The aim of this study was to comparatively assess the anti-inflammatory effect of the methanolic extracts of fresh ginger samples from Ghana and China and identify biomarker(s) that is/are diagnostic of acute inflammation via untargeted metabolomics. A phytochemical assessment of the ginger extracts was initially performed with high performance liquid chromatography- quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS). Low (200 mg/kg) and maximum (400 mg/kg) doses the ginger extracts from each country were assessed using Sprague-Dawley (SD) rats against groups for positive (indomethacin, 10 mg/kg) and negative controls (no drug). Blood samples were taken from the retro orbital vein at four time points: 1, 4, 8 and 24 h after induction of inflammation. The levels of tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) were determined in the plasma samples. The metabolomics study was also performed with HPLC-QTOF/MS and the data analyzed with MetaboAnalyst 3.0 and SIMCA 14.1. Phytochemical evaluation revealed marked differences in terms of the compounds deemed to have anti-inflammatory activities in the ginger samples from the two countries. Ginger extract from Ghana showed superior anti-inflammatory effects over that from China. In the metabolomics study, L-valine was identified as the diagnostic biomarker of acute inflammation from 8 differential metabolites identified.
研究领域:
植物学
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药理学
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代谢组学
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DOI
CHINESE JOURNAL OF CHEMISTRY
·
2024
·
IF 5.5
Ning Li,Shanshan Xiao,Xiaoxiong Jin,Ziyuan Song
Comprehensive Summary The structural and surface properties of nanomedicines play an important role in determining their biological fate. Surface chemistry of nanomedicines is one of the key parameters, where researchers used various strategies such as PEGylation to avoid the undesired clearance of nanoparticles (NPs) for enhanced targeting effect. Nevertheless, the fine-tuning of surface chemistry through polymer functionalization remains largely unexplored. In this concise report, we find that the incorporation of only 10 mol% valine residues into the surface-anchored poly( L -glutamic acid) significantly lowered the macrophage uptake of NPs. The introduction of other hydrophobic amino acid residues, however, increased the NPs internalization instead. The chirality and the side-chain structure of valine played an important role in the unexpected uptake behavior. We believe this work highlights the impact of slight changes of the surface-anchored polymer structure on the behavior of NPs, drawing people's attention to the careful design of surface chemistry to optimize the nanomedicine design.
研究领域:
生物医学工程
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纳米医学
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免疫学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Shang Wu,Jian Ji,Lina Sheng,Yongli Ye,Yinzhi Zhang,Xiulan Sun
Disinfectants are widely used in food production and environmental sanitation to prevent illness, but bacteria resistance to these disinfectants and co-resistance to antibiotics pose a threat to public health. This study investigated the impact of commonly used disinfectants on the resistance of Salmonella Typhimurium (ST) to disinfectants and antibiotics, and explored the metabolic mechanisms underlying the resistance changes. The results showed that subinhibitory concentrations of disinfectants had a minor impact on the resistance of ST to four disinfectants. However, chlorine-containing disinfectants stress enhanced bacteria resistance to ampicillin, while quaternary ammonium compounds stress increased resistance to tetracycline and gentamicin. Untargeted metabolomics analysis revealed significant changes in glutathione metabolism and lysine and valine degradation pathways after disinfectant exposure. Specifically, ST activated lysine decarboxylation, leading to a significant decrease in lysine levels after benzalkonium chloride exposure, while valine and leucine degradation pathways were activated by sodium hypochlorite stress. The addition of downregulated L-lysine and L-valine increased the sensitivity of ST to antibiotics, providing further evidence for the findings of metabolomics. This study provides guidance for the proper use of disinfectants in food processing and establishes a strategy based on metabolomics to control antibiotic-resistant bacteria.
研究领域:
微生物学
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代谢组学
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食品安全
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公共卫生
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产品关联
DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2020
·Vol. 77
·期 6
·1016-1023
·
IF 1.746
Yue Xue,Yan Bingyu,Wang Shuai,Gao Wen,Zhang Ruiyan,Dong Huijun
The glycopeptide A40926 biosynthesized by Nonomuraea gerenzanensis is a precursor of the second generation glycopeptide antibiotic dalbavancin. The skeleton of this glycopeptide consists of seven amino acids and is biosynthesized by the NRPS gene module. L-valine, a branched amino acid, is also a significant precursor for A40926 production. This study details the use of pH-responsive alginate–chitosan microspheres loaded with L-valine prepared by internal emulsification gelation. The effects of process and formulation variables on microsphere size, loading capacity, and encapsulation efficiency were investigated. Then, effects on A40926 production by the pH-responsive microspheres were evaluated in a 10-L fermenter. Results demonstrated that use of the pH-responsive microspheres could improve A40926 yield from 465 to 602 mg L −1 in a 10-L scale fermenter.
研究领域:
微生物学
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生物工程
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药学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Li Xin,Ma Yuntian,Wang Xin,Lü Xin
Macronutrients participate in vital biological processes and are critical in regulating organismal health. DL-norvaline, a nonproteinogenic amino acid, is an important pharmaceutical intermediate and a functional food ingredient for obesity control. This study aimed to explore the ameliorative effect and underlying mechanisms of DL-norvaline on lipid metabolism in high-fat diet-induced obese mice. The results showed that DL-norvaline inhibited adipogenesis and lipid accumulation in 3T3-L1 adipocytes, ameliorated tissue weight, food efficiency ratio and serum biochemical markers in obese mice. The combined transcriptomics and lipidomics analysis showed that lipid metabolism of adipose tissue such as sphingolipid and glycerophospholipid metabolism was ameliorated in obese mice after DL-norvaline treatment, particularly ceramides, via regulating peroxisome proliferators-activated receptor signaling pathway. This study offered a complementary perspective for exploring the effects of amino acids on lipid metabolism and speculated the possible mechanisms of ameliorating lipid-metabolism disorders by DL-norvaline in obese mice.
研究领域:
生物化学
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分子生物学
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营养学
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药理学
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产品关联
DOI
高影响因子
Acta Biomaterialia
·
2017
·
IF 6.319
Jun Deng,Mengyun Yao,Changyou Gao
Nanoparticles (NPs) can have profound effects on cell biology. However, the potential adverse effects of gold nanoparticles (AuNPs) with different surface chirality and structures have not been elucidated. In this study, monolayers of poly(acryloyl- l ( d )-valine ( l ( d )-PAV) chiral molecules were anchored on the surfaces of gold nanocubes (AuNCs) and nanooctahedras (AuNOs), respectively. The l -PAV-AuNCs and d -PAV-AuNCs, or the l -PAV-AuNOs and d -PAV-AuNOs, had identical physicochemical properties in terms of size, morphology and ligand density except of the reverse molecular chirality on the particle surfaces, respectively. The l -PAV capped AuNCs and AuNOs exhibited larger cytotoxicity to A549 cells than the D-PAV coated ones, and the PAV-AuNOs had larger cytotoxicity than PAV-AuNCs when being capped with the same type of enantiomers , respectively. The cytotoxicity was positively correlated with the cellular uptake amount, and thereby the production of intracellular reactive oxygen species (ROS). Statement of Significance • Gold nanoparticles with different structure and surface chirality are fabricated. • The structure and surface chirality at the nanoscale can influence cytotoxicity and genotoxicity. • A new perspective on designing nanoparticles for drug delivery, bioimaging and diagnosis.
研究领域:
纳米生物学
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毒理学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2012
·Vol. 125
·期 4
·3092-3099
·
IF 1.289
Suwen Ye,Licheng Tan,Yiwang Chen,Junchao Wei,Weihua Zhou
A series of aliphatic biodegradable poly(butylene succinate)- co -oligo( L -valine) (PBSVAL) copolyesters were synthesized with dimethyl succinate, 1,4-butanediol (BDO), and oligo( L -valine) (OVL) as reagents. The GPC results show that the weight average molecular weight ( M w ) of the copolyester with a feed ratio (PBS/OVL) of 0.9/0.1 (PBSVAL 10 ) is 4.9 × 10 4 g/mol, and its polydispersity index is 1.68. All the copolyesters were more thermally stable than the pristine poly(butylene succinate) (PBS). The incorporation of valine units into the copolyesters eventually led to the decrease of the crystallization temperature, melting temperature, while increase of crystallization enthalpy and melting enthalpy. The crystal structure of the copolyesters is similar to pure PBS except for higher degrees of crystallinity; also there was a trend to higher degrees of crystallinity with increasing content of valine. From the enzymatic degradation experiment, it is clear that the PBSVAL 10 copolyester shows less weight loss as compared to pure PBS which is due to PBSVAL 10 with higher degree of crystallinity. The morphology of the copolyester films after degradation was also studied by the scanning electron microscopy. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Jibao Zhang,Yan Li,Zhenyuan Yin,Praveen Linga,Tianbiao He,Xiang Yuan Zheng
Hydrogen (H 2 ) emerges as a promising future clean energy for the low-carbon and sustainable future due to its high energy density and no pollutant or greenhouse gas emissions after combustion. The advantage of H 2 storage in solid-hydrate form is the requirement of only moderate pressure and temperature compared to compressed H 2 storage and the relatively lower energy consumption compared to liquid H 2 storage. However, the slow kinetics of H 2 hydrate formation is one major challenge to breakthrough for the large-scale development of hydrate-based H 2 storage. In this study, we introduced an environmental-benign kinetic promoter ( L -valine, L -Val) and coupled it with a thermodynamic promoter tetrahydrofuran (THF) using stoichiometric concentration (5.56 mol%) to enhance H 2 hydrate formation kinetics. 0.3 wt% L -Val coupled with 5.56 mol% THF yields the optimal kinetic enhancement, and the maximum volumetric H 2 uptake ( V H2 ) is 29.83 ± 1.22 volume of H 2 /volume of H 2 -THF hydrate (v /v). The reusability of L -Val is proved in five consecutive cycles of H 2 -THF hydrate formation and dissociation with consistently superior H 2 uptake. Furthermore, it was identified that cooling rate ( R c ) was an important factor determining the H 2 -THF hydrate formation kinetics. Lower R c (0.05 K/min) shows an optimal promoting effect of V H2 , which was 105.29% higher than that at higher R c (0.3 K/min). A mechanism is proposed to explain the effect of R c on H 2 -THF hydrate formation kinetics. The maximum gravimetric H 2 ( G H2 ) reported in our study reaches 0.26 ± 0.01 wt%, which was superior in current literature below 15.0 MPa. The results provide insights into speeding up H 2 uptake in solid-hydrate form and have potential applications in hydrate-based hydrogen storage.
研究领域:
化学工程
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能源存储
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材料科学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Huibo Chi,Yan Gu,Tingting Xu,Feng Cao
To study the cellular uptake mechanism of multifunctional organic–inorganic hybrid nanoparticles and nanosheets, new chitosan–glutathione–valine–valine-layered double hydroxide (CG-VV-LDH) nanosheets with active targeting to peptide transporter-1 (PepT-1) were prepared, characterized and further compared with CG-VV-LDH nanoparticles. Both organic–inorganic hybrid nanoparticles and nanosheets showed a sustained release in vitro and prolonged precorneal retention time in vivo, but CG-VV-LDH nanoparticles showed superior permeability in the isolated cornea of rabbits than CG-VV-LDH nanosheets. Furthermore, results of cellular uptake on human corneal epithelial primary cells (HCEpiC) and retinal pigment epithelial (ARPE-19) cells indicated that both clathrin-mediated endocytosis and active transport of PepT-1 are involved in the internalization of CG-VV-LDH nanoparticles and CG-VV-LDH nanosheets. In summary, the CG-VV-LDH nanoparticle may be a promising carrier as a topical ocular drug delivery system for the treatment of ocular diseases of mid-posterior segments, while the CG-VV-LDH nanosheet may be suitable for the treatment of ocular surface diseases.
研究领域:
药学
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纳米技术
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眼科学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Zhongqian He,Huaming Yu,Dongping Chen,Xuyan Ni,Chunshuang Yan,Chade Lv,Yuejiao Chen
Graphical Valine was introduced as an aqueous electrolyte additive. The functional groups within the additive molecules were utilized to stabilize the electrode/electrolyte interface (EEI), thereby achieving a reversible dendrite-free zinc anode. The potential for scale-up application has been acknowledged by researchers for rechargeable aqueous zinc-ion batteries (ZIBs). Nonetheless, the progress of the development is significantly impeded due to the instability of the interface between the zinc anode and electrolyte. Herein, efficient and environmentally benign valine (Val) were introduced as aqueous electrolyte additive to stabilize the electrode/electrolyte interface (EEI) via functional groups in additive molecules, thus achieving reversible dendrite-free zinc anode. The amino groups present in Val molecules have a strong ability to adsorb on the surface of zinc metal, enabling the construction of anchored molecular layer on the surface of zinc anodes. The strongly polar carboxyl groups in Val molecules can act as ion-transport pumps to capture zinc ions in the electric double layer (EDL) through coordination chemistry. Therefore, this reconstructed EEI could modulate the zinc ion flux and simultaneously suppress side reactions and dendritic growth of Zn. Consequently, a long stable cycling up to 1400 h at a high current density of 20 mA cm −2 is achieved. Additionally, Zn//V 2 O 5 full cell with Val additive exhibit enhanced cyclability, retaining 77 % capacity after 3000 cycles, displaying significant potential in promoting the commercialization of ZIBs.
研究领域:
材料科学
·
电化学
·
能源存储
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Deng Jun,Wu Sai,Yao Mengyun,Gao Changyou
Chirality is one of the ubiquitous phenomena in biological systems. The left handed (L-) amino acids and right handed (D-) sugars are normally found in proteins and in RNAs and DNAs, respectively. The effect of chiral surfaces at the nanoscale on cellular uptake has, however, not been explored. This study reveals for the first time the molecular chirality on gold nanoparticles (AuNPs) functions as a direct regulator for cellular uptake. Monolayers of 2-mercaptoacetyl-L(D)-valine (L(D)-MAV) and poly(acryloyl-L(D)-valine (L(D)-PAV) chiral molecules were formed on AuNPs surface, respectively. The internalized amount of PAV-AuNPs was several times larger than that of MAV-AuNPs by A549 and HepG2 cells, regardless of the chirality difference. However, the D-PAV-AuNPs were internalized with significantly larger amount than the L-PAV-AuNPs. This chirality-dependent uptake effect is likely attributed to the preferable interaction between the L-phospholipid-based cell membrane and the D-enantiomers.
研究领域:
纳米技术
·
细胞生物学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2021
·
IF 3.764
Wei Li,Jing Yang,Shichao Du,Estevao Macaringue,Yan Wang,Songgu Wu,Junbo Gong
l-Valine crystallizes in a flake-like shape generally; however, compared with the flake-like products, spherulitic l-valine has better filterability, flowability, and bulk density and has potential for wider industrial applications in the future. In this study, l-valine spherulites have been successfully prepared by an evaporative crystallization method in the presence of no more than 0.5% hydroxypropyl methyl cellulose (HPMC). The spheroidization of l-valine proceeds via a layer-by-layer transformation from an initial flake-like shape to a petal-like form, and then it continues to grow into the final sphere-like shape. Molecular dynamics simulations show that HPMC interacts with hydrophilic (001) faces preferentially and promotes the continuous layer growth process. Only the substituent groups of hydroxypropyl and methyl exist simultaneously within a certain spatial distance and, in a viscosity of 40–50 mPa·s, can promote the formation of l-valine spherulites. The particle size increased with the increase in the HPMC dosage and decrease of temperature or evaporation rate. This effect of HPMC promoting the spherical formation provides a method to enrich the design of spherulites and new insights into the fabrication mechanism of spherulitic growth.
研究领域:
化学
·
材料科学
·
晶体学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）