BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2025
·
IF 2.7
Maobin Xie,Dingshan Zhang,Jiayu Liu,Yao Jiang,Jin Bai,Hai Qiao
The aggregation of suspended particles caused by the coffee-ring effect (CRE) disrupts uniform distribution and limits accurate transmission electron microscopy (TEM) observation. Here, we show that glutaraldehyde (GA) suppresses CRE in aqueous polystyrene microsphere droplets and enables more uniform outer membrane vesicles (OMVs) deposition for TEM. Specifically, the addition of GA (0%–15% v/v) to 1% w/v, 1 µm red polystyrene microsphere suspensions progressively reduced ring formation: The ratio of maximum-to-minimum gray values decreased from 2.40 ± 0.22 (0% GA) to 1.11 ± 0.06 (15% GA), and mean CRE width dropped from ∼97 to ∼3.2 µm. Microsphere radial velocity decreased from 4.25 ± 0.37 (0% GA) to 0.89 ± 0.08 µm/s (15% GA), whereas bulk solution viscosity increased concomitantly (from ∼4 to ∼232 mPa s at 15% GA), indicating viscosity-mediated suppression of radial capillary flow. Addition of sodium dodecyl sulfate (SDS) partly restored CRE, consistent with reduction of surface-tension gradients that drive Marangoni reflux. Applying GA (6% v/v) to OMVs TEM preparation produced homogeneous OMVs counts across center, middle, and edge regions, without observable morphological alteration. We conclude that GA suppresses CRE primarily by (i) increasing viscosity to inhibit radial capillary flow and (ii) establishing surface-tension-driven Marangoni backflow that redistributes particles away from the contact line. GA thus provides a practical, fixation-compatible approach to improve TEM sample uniformity for biological nanoassemblies.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Kai Zheng,Zhaomiao Liu,Siyu Zhao,Nan Zheng,Chenchen Zhang,Yan Pang,Xiang Wang
Precise control of cell lateral positions in microchannels is critical for high-accuracy cell isolation, with such positioning primarily governed by lateral migration. Among the various factors influencing lateral migration, interphase forces play a key but underexplored role. In this study, a simulation model was developed to resolve the evolution of interphase interaction during cell–cell collisions. Using this model, we systematically analyze cell migration under the combined influence of lateral lift forces and interphase force. The results show that under low Reynolds number (Re) conditions, lateral lift forces dominate, and cells gradually reach equilibrium positions through repeated collision and separation. As Re increases, however, collisions occur with greater relative velocity, generating stronger interphase forces that drive rapid separation and induce transient but pronounced shifts in lateral cell position, typically persisting for only a few milliseconds, with peak displacements of about 25% of the equilibrium position. Experimental validation of the simulation results is achieved through microcapillary flow tests with blood cells and particles, supporting the simulation model’s predictive accuracy. Notably, we observe the formation of transient secondary rings under high Re conditions, which are attributed to interphase interactions. This study delivers mechanistic insights into intercellular dynamics in confined microflows and establishes a rigorous modeling framework for the design of high-throughput, high-purity cell isolation systems.
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AICHE JOURNAL
·
2025
·Vol. 72
·期 2
·e70145
·
IF 4
Liang Yuan,Yao Wu,Hai-feng Cong,Xin-gang Li
Helical liquid-bridge flow, a novel semi-constrained flow, shows significant potential for enhancing gas-liquid mass transfer and reactions. Research confirms millimeter-scale spiral bridges offer high efficiency and low pressure drop in distillation, absorption, and reaction, while micrometer-scale flow enables controllable gas-liquid contact in microchemical engineering. However, behavioral differences across scales remain unclear, and quantitative models for spiral design and flow prediction are lacking. This study systematically investigated flow characteristics and morphology evolution in millimeter and micrometer liquid bridges. Then, a multi-parameter flow model was established based on the experiments. In addition, the consistency was improved from 0.825 to 0.996 by minimizing the surface energy optimization model. The model can accurately predict key parameters such as the liquid bridge profile, providing a reliable theoretical basis and effective methods for the selection and optimization of helical structures in gas-liquid mass transfer and reaction processes at different scales.
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PROGRESS IN ORGANIC COATINGS
·
2025
·
IF 7.3
Jingwen Zhao,Peng Zhu,Huapeng Zhang
At present, hydrophobic surfaces with simple polymer array structures such as hemispheres, columns, trapezoidal steps, or cones (with a small top and a large bottom) can be obtained by direct negative moulding. However, complex structures, such as spherical, with large upper and small lower structures, are difficult to be obtained by negative moulding at once. Here, a new preparation strategy for hydrophobic epoxy resin coatings containing spherical microparticle arrays was proposed, using a surface porous polystyrene (PS, solvent soluble) film template-assisted negative moulding. Firstly, the preparation process of surface porous polystyrene films by dry casting from a ternary PS/polyethylene glycol (PEG)-toluene mixture would be optimized in depth, such as the dissolution & dispersion method, humidity, total concentration of polymer, PEG molecular weight, and PS/PEG mass ratio. Secondly, the structures and the water contact angle of the subsequently obtained epoxy resin array coatings would be comparatively studied with those of surface porous PS films. This work will provide a simple, efficient, and inexpensive preparation route for epoxy resin coatings containing spherical microparticle arrays.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Jingyi Guo,Na Liu,Jingfu Liu,Jianjian Wu,Fei Ge
Microplastics (MPs) act as vectors for co-existing pollutants, altering their environmental behavior and ecological risks. Yet the impact of MPs on the chronic effects of co-existing pollutants remains poorly understood. Herein, we explored the role of polystyrene (PS) MPs in facilitating the transgenerational transfer (F 0 -F 4 ) and reproductive toxicity of zinc oxide nanoparticles (ZnO NPs) in Daphnia magna ( D. magna ) under chronic dietary exposure. Results showed that PS MPs facilitated the transgenerational transfer of ZnO NPs, resulting in prolonged transport of ZnO NPs by one generation. This was attributed to that PS MPs impaired Zn excretion of D. magna via significantly inhibiting the molting rates by 40% ( p < 0.05), promoting the transfer of Zn to offspring. Besides, confocal microscopy confirmed Zn enrichment in the ovum and umbilical cords. Molecular docking simulation revealed stable Zn 2 + binding to vitellogenin (VTG) protein via hydrogen bonds (-1200 kJ/mol), indicating VTG-mediated maternal transfer of Zn. Furthermore, PS MPs facilitated the multi-generational reproductive impact of ZnO NPs to extend to the fourth generation (F 3 ). Transcriptomic analysis identified significant inhibition of prolactin signaling pathways, with the key genes Signal Transducer and Activator of Transcription ( STAT ) and VTG decreasing by 80.4% and 7.8% in the female generation (F 0 ), respectively, inhibiting oocyte development. Overall, these findings confirmed that MPs facilitated the reproductive toxicity and transgenerational transfer of co-existing pollutants, providing critical insights for long-term ecological risk assessment of MPs and co-existing pollutants combined exposure in aquatic systems.
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LAB ON A CHIP
·
2026
·
IF 6.3
Xian Chen,Chuanchao Zhang,Yaping Wang,Xuexin Duan,Yunhua Gao
Micro-mixing plays an important role in the biochemical analyses using miniaturized total analysis system (µTAS) or lab-on-chips. However, the existing micromixers primarily rely on the specific medium and lack the capability of being activated on-demand, and it is rather challenging to ensure the adequate analyte capture and fluid washing on the surface of each probe particle during the assay steps. Herein, we demonstrated a novel acoustic chaotic micromixer actuated by a Lamb wave resonator (LWR) array, which integrated all steps from the initial sample pretreatment, chaotic probe particle mixing, analytes trapping, fluid washing to the ultimate signal acquisition, thereby fulfilling the “sample-in-answer-out” medical needs. The high-speed microscale acoustic streaming vortices triggered by the four LWRs in the confined micro-channel can be utilized to accelerate the particles’ velocity and enhance the collision between probe particle and target analyte. In addition, chaotic particle mixing effect was realized by on-demand controlling the direction and magnitude of the four-in-one drag force based on the programmable acoustic stimuli of each independent LWR. Besides, a compact cartridge was specifically designed to accommodate the acoustic chaotic micromixer and peripheral accessories (including RF signal source, CMOS optical sensor and mini pump), thus presenting a handheld device for point-of-care applications. Finally, the trace prostate specific antigen (PSA) derived from fingertip blood samples was effectively detected. Owning to the compact size, on-chip chaotic particle mixing, fast and sensitive detection performance, the proposed acoustofluidic system is very suitable for the on-site diagnostic applications.
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SmartMat
·
2026
·Vol. 7
·期 1
·e70064
·
IF 14.3
Shuai Zhang,Kezhou Chen,Lin Xi,QingXin Luan,Jiayi Liu,Tiegen Liu,Lili Hou
Optically switchable functions are highly interesting for AI applications in smart displays, memory encryption, and neuromorphic computing. The use of molecular photoswitches to precisely modulate functionalities at the nanoscale, such as photoluminescence (PL), is essential for developing photoswitchable nanomaterials. However, it is challenging to achieve the universal regulation of full-color PL, particularly for the efficient optical modulation while maintaining both high PL quantum yield (PLQY) and effective photoswitchable states. Herein, we report a simple and efficient photoswitchable system achieved through the hybridization of quantum dots (QDs) and diarylethene (DAE) photoswitches. The PL intensity of the three primary colors (RGB) can be reversibly switched on and off using the same DAE upon exposure to specific light. Our design exhibits a maximum PLQY exceeding 90% in the on state while maintaining a PL on/off ratio approaching 100, which represents one of the current highest values. The excellent performance relies on the synergistic energy transfer mechanism of the Förster resonance energy transfer and triplet energy transfer. Our photoswitchable materials enable the straightforward preparation of RGB QDs@DAE films for applications in reversible patterned display and information encryption. The patterns and information can be erased and rewritten multiple times, offering flexible and reusable smart materials toward data storage, advanced display, and anti-counterfeiting technologies.
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ADVANCED MATERIALS
·
2026
·
IF 29.1
Lihao Guo,Youbin Zheng,Shuxiang Xu,Bingbing Feng,Kan Wang,Rou Huang,Gandong Zhou,Chutong Liu,Rawan Omar,Danyao Qu,Jinbao Li,Min Zhang,Weiwei Wu,Guangjian Zhang,Lina Huang,Hossam Haick,Miaomiao Yuan
Long-term monitoring of electrolyte dynamics is essential for managing chronic diseases. Conventional diagnostic tests are steadily evolving toward greater convenience, personalization, and accuracy, while wearable microneedle sensors offer a compelling alternative. However, their long-term biocompatibility is hampered by the mechanical stiffness required to penetrate the skin. Here we report a crosslinking–recombination bovine serum albumin (CR-BSA) coating that undergoes a unique stiff-to-soft transition, reconciling insertion capability with tissue compatibility. For Na + sensing, CR-BSA serves as a functional buffer layer, delivering high sensitivity (70.6 µA/decade) and stability for more than 14 days, while significantly reducing inflammation and fibrosis compared with commercial Nafion coatings. CR-BSA–coated microneedle sensors achieve reliable continuous monitoring of both acute and chronic hyponatremia and hypernatremia for over five days. This stiff-to-soft coating strategy overcomes a central barrier to microneedle sensor integration, advancing the prospects of long-term, minimally invasive electrolyte monitoring for chronic disease management.
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ACS Applied Nano Materials
·
2026
·
IF 5.5
Shao-Ye Su,Liu-Liu Yang,Xin-Rui Shi,Fa-Wen Wu
The practical application of metal–organic frameworks (MOFs) in water remediation is often hindered by their powdery nature, which causes aggregation, difficult recovery, and potential secondary pollution. To address this, we pioneer an integrated strategy of chemical bonding and in situ epitaxial growth to fabricate, for the first time, a robust and substrate-anchored cyclodextrin-based MOF (CD-MOF) composite. The innovation centers on using ethylenediamine-modified β-cyclodextrin (EDA-β-CD) as chemically grafted nucleation sites on aldehyde-functionalized polystyrene to guide the oriented growth of CD-MOF, yielding a well-adhered PS-CD-MOF composite. This novel material exhibits excellent adsorption of sulfonamide antibiotics (e.g., 5.55 μg·mg–1 for sulfadimethoxine) with rapid kinetics. More importantly, it demonstrates outstanding reusability over multiple cycles while maintaining structural integrity, thereby overcoming a key limitation of powder MOFs. Its versatile adsorption across different sulfonamides confirms the success of the synthesis strategy and highlights its potential as a stable, recyclable platform for waste liquid.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Zhongwei Wang,Zhu Liu,Yipeng Tang,Wulin Yang,Yan Qing,Yiqiang Wu,Shoulu Yang
Most industrial adhesives are derived from petroleum-based feedstocks, raising concerns about sustainability and environmental impact. Although bio-based alternatives have been explored, they often suffer from limited mechanical strength and poor water resistance. In this study, a high-performance bio-based epoxy adhesive was developed using raw black liquor as a direct source of kraft lignin. The synthesis involved epoxidation followed by cross-linking polymerization with diethylenetriamine and maleic anhydride. After thermal curing at 180 °C for 2 h, the adhesive exhibited an exceptional lap shear strength of 32 MPa on aluminum substrates, outperforming all previously reported lignin-based adhesives and even most commercial adhesives. This enhanced adhesion is attributed to structural amido-ester covalent bonds in the adhesive, as well as the multiple three-dimensional hydrogen bond networks formed between the hydroxyl/amino groups on the amido-ester chains and the substrate interface. The adhesive also demonstrated a level of water resistance. The experimental results were strongly supported by DFT and MD simulations, which confirmed the role of interfacial hydrogen bonding in adhesion. This work presents a scalable approach for valorizing lignin-rich industrial waste streams into advanced adhesive systems with competitive performance.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Huining Zhang,Baixiang Wang,Yakun Dong,Jianping Han,Yi Zhao
To effectively address the potential threats posed by microplastics (MPs) to the environment and human health, this study drew inspiration from the naturally occurring biocompatible substance dopamine. We prepared poly(dopamine) (PDA)-modified graphene oxide (GO) ink and successfully constructed a poly(dopamine)-functionalized graphene macro-adsorption network (3DGO/PDA) via direct ink writing (DIW) printing technology. The incorporation of PDA significantly enhances the adsorption active sites of 3DGO/PDA, endowing it with outstanding adsorption performance toward polystyrene (PS) microspheres. Concurrently, the exceptional three-dimensional network pore structure of 3DGO/PDA provides efficient mass transfer pathways and abundant pore space for the adsorption and storage of polystyrene. Experimental results indicate that under pH= 5 conditions, 3DGO/PDA3 achieves an 86 % (PS initial concentration of 200 mg/L, temperature of 313.15 K) removal rate for polystyrene, maintaining stable adsorption performance after multiple cycles. Density functional theory (DFT) calculations indicate that the adsorption of PS onto the adsorbent involves a synergistic interaction of multiple mechanisms, with amino-dominated chemical adsorption playing a dominant role. Therefore, by combining the functionalization design of polydopamine with 3D printing technology and leveraging density functional theory calculations at the electronic level, the enhanced adsorption mechanism was systematically elucidated. This provided new design ideas and a theoretical basis for the development of efficient microplastic adsorption materials.
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Ruidan Li,Li Tian,Yujia Song,Yanjia Guo,Guangping Ma,Pengfei Han,Hanyue Jiang,Wenzhuo Wang,Tengying Ma
In this paper, a dual-mode electrochemiluminescence-electrochemical (ECL-EC) sensor was constructed using Ru(bpy) 3 2+ @Ti 3 CN and Zn 4 Sn 3 O 5 via multi-step amplification strategy for the detection of sodium saccharin (SS). The maximum ECL/EC initial signals were obtained by the synergistic action of Ru(bpy) 3 2+ @Ti 3 CN and Zn 4 Sn 3 O 5 in the tri-n-propylamine (TPrA) solution. Given that SS reacted with Ru(bpy) 3 2+ , the ECL/EC signal values both decreased. The relevant mechanism was investigated. Under the best optimization conditions, the ECL detection exhibited a linear range of 5.0 × 10 −15 to 5.0 × 10 −10 mol·L −1 with a limit of detection (LOD) of 1.67 × 10 −15 mol·L −1 (S/N = 3), while EC detection showed a linear range of 5.0 × 10 −9 to 5.0 × 10 −4 mol·L −1 with the LOD of 1.67 × 10 −9 mol·L −1 (S/N = 3). Satisfactory results were obtained in a variety of actual samples.
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