MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Su Han Wang,Min Lin,Jing Liu,Jian Ping Wang,Ning Peng Wu
The quinolone efflux pump (QepA) protein of a fluoroquinolones-resistant Escherichia coli was grafted on Fe 3 O 4 to synthesize a type of magnetic composite Fe 3 O 4 @QepA. This material was combined with 4 fluorescent tracers to develop a semi-homogeneous fluorescent array on 96-well microplate for determination of fluoroquinolones (FQs) in chicken, and the results were analyzed by using linear discriminant analysis method. Results showed this method could discriminatively determine 15 FQs at the concentration ≥10 ng/g, and could identify each drug species at the concentration ≥2 ng/g. The method exhibited satisfactory recoveries in chicken sample (61.7 %–119.8 %). The analysis results for some unknown samples were consistent with a liquid chromatography mass spectrometry method. This is the first paper reporting a semi-homogeneous fluorescent array for discriminative determination of small molecule substances, and this array can be used as an accurate tool to determine the 15 FQs in meat sample.
研究领域:
分析化学
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微生物学
·
食品安全
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产品关联
DOI
高影响因子
Optoelectronics Letters
·
2024
·Vol. 20
·期 12
·741-748
·
IF 0.8
Zulpiye Hasanjan,Dou Xiaozong,Zhang Hongyan
In this study, a water-soluble fluorescent probe based on copper nanoparticles (CuNPs) was synthesized using a hydrothermal method with bovine serum albumin (BSA) (BSA@CuNPs) as a protectant, and it was utilized to efficiently detect the concentration of tetracycline (TC). Upon the addition of TC to the BSA@CuNPs probe, its fluorescence intensity is obviously diminished due to the keto-enol functions C=O group in TC connects with the hydroxyl group on the BSA@CuNPs probe to form a non-fluorescent complex, which can be successfully utilized for detecting TC concentration. Linear range and detection limit for TC detection are 0.1–130 µM and 90 nM. Furthermore, the BSA@CuNPs probe was applied to detect TC concentrations in real meat samples, with recoveries ranging from 95.40% to 102.13%, and the relative standard deviation ( RSD ) was below 2.73%. Therefore, BSA@CuNPs probe exhibits high sensitivity and selectivity for TC, which will be used for the study of TC analysis in meat.
研究领域:
分析化学
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食品科学
·
纳米技术
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DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 22
·5011-5017
·
IF 5.227
Jiaxi Yin,Hairong Cui,Ling Lei,Kangbing Wu
Graphene nanosheets (GS) were prepared by ultrasonic exfoliation of bulk graphite in N-methyl-2-pyrrolidone with the assistance of sodium pyrophosphate. The obtained GS suspension was modified on a glassy carbon electrode (GS/GCE), and then functionalized at different voltages (e.g. 1.0, 1.4 and 1.6 V) for 2 min in pH 7.0 phosphate buffer. The electrochemically functionalized GS/GCE (i.e. EGS/GCE) possesses more oxygen-containing groups and a higher defect level. More importantly, the active response area, electron transfer ability and interface adsorption capacity of the EGS/GCE enhanced remarkably. The possible mechanism of the performance enhancement is discussed, and the sensing application of the EGS/GCE in the detection of nitrofurazone (NFZ) is investigated. Compared with the GS/GCE, the EGS/GCE is much more active for NFZ oxidation and greatly increases the detection sensitivity. As a result, a highly sensitive electrochemical detection method has been developed for NFZ, with a detection limit of 2.1 nM. The practical application of the EGS/GCE was tested in fish meat samples, showing good accuracy and feasibility.
研究领域:
分析化学
·
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Zhiming Yan,Biqing Hu,Qianlian Li,Sunxian Zhang,Jie Pang,Chunhua Wu
In this work, covalent organic frameworks SNW-1 incorporated polyacrylonitrile (SNW-1@PAN) nanofiber was synthesized and used as pipette tip solid phase extraction (PT-SPE) adsorbent for preconcentration of five sulfonamides (SAs) in meat samples. The SNW-1@PAN nanofiber was fabricated via two steps, including the hydrothermal synthesis of SNW-1 and co-electrospinning of the SNW-1/PAN mixture. The three dimensional networks of electrospun nanofiber provide both low backpressure and rapid mass transfer in PT-SPE application, thus preventing the problems of high backpressure and leakage caused by directly packing SNW-1 into PT-SPE cartridge. The SNW-1@PAN nanofiber packed PT-SPE cartridge was used to extract SAs in meat samples followed by high performance liquid chromatography (HPLC). The effects of different parameters influencing the extraction and desorption efficiency were intensively investigated and optimized. Under the optimum conditions, the linear ranges of SAs were in the range of 5–125 ng mL −1 with correlation coefficients above 0.998, and the limits of detection of the five SAs ranged from 1.7 to 2.7 ng mL −1 . The interday and intraday precision (RSD) for five replicate extractions of the five SAs (50 ng mL −1 for each) was in the range of 5.4–9.2%. The applicability of the proposed method was examined by analyzing the SAs in the pork and chicken samples. The recoveries of the five SAs varied in the range of 86–111% with good precision, showing the good capability of the proposed method for the determination of SAs in the complicated real samples.
研究领域:
分析化学
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材料科学
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食品科学
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 10
·1938-1946
·
IF 2.667
Pang Yue-Hong,Sun Miao,Shen Xiao-Fang,Yue Qi,Ma Tian-Tian,Yang Cheng
Benzo[a]pyrene (BaP) is a kind of trace pollutant commonly found in environment, food, and organism. It has been widely concerned because of its strong carcinogenicity to the human body. In this paper, covalent organic framework TpBD, in situ growth on titanium wire, was used as the coating of solid-phase microextraction (SPME) fiber and coupled with gas chromatography–flame ionization detector (GC-FID) for the detection of trace BaP in roast meat samples. The linear range of the developed method under the optimized experimental conditions was 0.02–15 μg L −1 , the detection limit (S/ N = 3) was 0.006 μg L −1 , and the limit of quantification (S/ N = 10) was 0.02 μg L −1 . The intra-day and inter-day precision of a single fiber were 1.73% and 2.27%, respectively. The relative standard deviation (RSD) between fiber-to-fiber was investigated by three as-fabricated fibers was 6.09%. Moreover, the enrichment factor of the fabricated TpBD SPME fiber for BaP was 4914, and the fiber can withstand at least 150 cycles of adsorption and desorption without significantly decrease in extraction efficiency (< 3.6%). Finally, the developed method was successfully applied to the detection of BaP in roast meat samples with the recoveries from 95.4 to 108.5%.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
高影响因子
ELECTROPHORESIS
·
2020
·Vol. 42
·期 4
·465-472
·
IF 3.081
Qiuyue Xu,Beibei Chen,Man He,Bin Hu
Ti (IV)-modified vinyl phosphate magnetic nanoparticles (Fe 3 O 4 @SiO 2 @KH570-PO 4 -Ti (IV)) was prepared for simultaneous extraction of multiple arsenic species, followed by high performance liquid chromatography (HPLC)– inductively coupled plasma mass spectrometry (ICP-MS) analysis. Inorganic arsenic (iAs), dimethyl arsenic acid (DMA), monomethyl arsenic acid (MMA), p -amino phenyl arsenic acid ( p -ASA), 4-hdroxyphenylarsenic acid (4-OH), phenyl arsenic acid (PAA), and 3-nitro-4-hydroxyphenylarsenic acid (ROX) were investigated as interest analytes. It was found that they were quantitatively adsorbed on Fe 3 O 4 @SiO 2 @KH570-PO 4 -Ti (IV) at pH 5, and desorbed completely with 0.1 mol/L sodium hydroxide solution. Enrichment factor of 100-fold was obtained by consuming 100 mL sample solution. Under the optimal conditions, the method combining MSPE with HPLC-ICP-MS presented a linear range of 1–5000 ng/L for seven arsenic species. The limits of detection were 0.39, 0.60, 0.23, 1.85, 0.54, 0.48, and 0.84 ng/L for DMA, MMA, p -ASA, iAs, 4-OH, PAA, ROX, with the relative standard deviations ( c = 10 ng/L, n = 7) of 3.6, 3.9, 5.5, 12.4, 6.1, 5.8, 5.0, respectively. The accuracy of the method was validated by analyzing BCR 627 Tuna fish. The application potential of the method was further evaluated by chicken muscle and liver samples. No target arsenic species were detected in these samples, and good recoveries (80.6–123%) were obtained for the spiked samples at low, medium, and high concentration levels.
研究领域:
分析化学
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环境科学
·
食品科学
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DOI
高影响因子
Food Analytical Methods
·
2018
·Vol. 12
·期 1
·1-11
·
IF 2.245
Chang Qingyun,Zang Xiaohuan,Wu Tong,Wang Mengting,Pang Yachao,Wang Chun,Wang Zhi
A functionalized covalent organic framework (COF) was synthesized via room-temperature solvent-free mechano-chemical grinding method and used as a solid-phase extraction adsorbent for the extraction of eight biogenic amines (tryptamine, n-butylamine, phenylethylamine, cadaverine, putrescine, tyramine, spermine, and spermidine) in diverse meat samples. The target biogenic amines were derived with dansyl chloride to form stable fluorogenic derivatives for their high-performance liquid chromatography–fluorescence detection. The main important experimental parameters were investigated and optimized by both one-factor-at-a-time and response surface methodologies. Under the optimum conditions, a linear response for the biogenic amines was observed in the concentration range of 5.0–800.0 μg L −1 with the coefficients of determination ( r 2 ) ranging from 0.9944 to 0.9981. The limits of detection of this method (at a signal-to-noise ratio of 3) for the analytes ranged from 0.92 to 2.57 μg L −1 . The method recoveries of the target biogenic amines for five meat samples ranged from 80.3 to 115%, with relative standard deviations lower than 12%. The results indicate that the prepared COF is an effective adsorbent for the enrichment of biogenic amines.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Tian-Tian Ma,Xiao-Fang Shen,Cheng Yang,Hai-Long Qian,Yue-Hong Pang,Xiu-Ping Yan
Covalent organic framework TpBD was grafted on stainless steel wire with polydopamine as a linker. The fabricated TpBD bonded stainless steel wire was used as the solid-phase microextraction fiber to extract sixteen polycyclic aromatic hydrocarbons (PAHs) for subsequent GC-MS/MS determination in grilled meat samples. The developed method gave the limits of detection ( S/N = 3) from 0.02 (pyrene)-1.66 (naphthalene) ng L −1 and enhancement factors from 1069 (naphthalene)-10879 (benz(a)anthracene). The relative standard deviations (RSDs) for intra-day and inter-day study are in the range of 2.6%–8.5% and 4.5%–9.4%, respectively. The fiber-to-fiber RSDs for three parallel prepared fibers were 5.3%–10.0%. One TpBD bonded fiber can stand at least 200 cycles without significant loss of extraction efficiency. The developed method was successfully applied for the determination of trace PAHs in grilled meat samples with recoveries from 85.1% to 102.8%.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 41
·5025-5031
·
IF 2.073
Teng Zhang,Jing Liu,Jian Ping Wang
The residues of amantadine and rimantadine in foods of animal origin are dangerous to consumers. This study used adamantane as the dummy template for the first time to synthesize a molecularly imprinted polymer capable of simultaneously recognizing amantadine and rimantadine. This polymer was used as the recognition reagent to prepare a chemiluminescence sensor on the conventional 96-well microtiter plate for the determination of the two drugs in chicken and pork samples. The results showed that one test could be finished within 20 min, and the sensor could be reused 6 times. Due to the 4-(imidazol-1-yl)phenol enhanced luminol–H2O2 signal system, the limit of detection for the two analytes was 1.0 pg mL−1. The recoveries of the two analytes from the standard fortified blank meat samples were in the range of 67.2–93.5%. Therefore, the sensor could be used as a simple, rapid, sensitive, specific and recyclable tool for the routine monitoring of the residues of amantadine and rimantadine in meat.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 145
·期 14
·5011-5018
·
IF 3.978
Xinyang Zhang,Jianan Wen,Lili Lian,Xianhong Ma,Xiyue Wang,Dawei Lou
3D magnetic porous carbon (MPCK) was prepared using the metal–organic framework (MOF) MIL-100(Fe) as the carbon precursor by carbonisation and KOH activation strategies. Carbonisation and activation ensured that MPCK possessed excellent structural and thermal stability, strong magnetic responsiveness and high surface area. MPCK was used as an adsorbent for the magnetic solid-phase extraction of clenbuterol and ractopamine from mutton samples. The concentration levels of analytes were determined by ultra-high performance liquid chromatography-mass spectrometry. Under optimised extraction conditions, the peak area responded linearly to analytes over the concentration range from 0.05 μg L−1 to 40 μg L−1 (r ≥ 0.9972). The detection limits of clenbuterol and ractopamine were found to be 0.130 μg kg−1 and 0.150 μg kg−1, respectively. The satisfactory recoveries in mutton samples ranging from 95.64% to 114.65% indicated that 3D porous carbon is a promising adsorption material for the extraction of clenbuterol and ractopamine from complex biological matrixes.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Wei Zhang,Peipei Zhou,Wei Liu,Huili Wang,Xuedong Wang
Owing to matrix complexity, the development of green and convenient pretreatment methods is crucial for routine monitoring of organic contaminants in meat samples. Dicationic ionic liquids (DILs) possess two active centers, thus resulting in superior physicochemical properties over monocationic species. Herein, the hydrophilic DILs and in-situ metathesis reaction salt were introduced into NiFe 2 O 4 -based magnetic effervescent tablets for enhancing adsorption/extraction of five typical polycyclic aromatic hydrocarbons (PAHs) in meats. Also, the synthesized NiFe 2 O 4 nanoparticles displayed larger specific surface area than others (CoFe 2 O 4 , Fe 3 O 4 and ZnFe 2 O 4 ), thereby supplying higher adsorption capacity for PAHs. The integration of DILs with NiFe 2 O 4 into magnetic effervescent tablets realized highly efficient adsorption/extraction, rapid magnetic separation/retrieval and CO 2 -induced vigorous dispersion into one synchronous step. After hydrolysis of the ground meat samples using 10 μM KOH, the filtered solution was diluted and subjected to magnetic effervescent tablet-assisted microextraction procedures. Optimized experimental variables were as follows: an effervescent tablet containing 0.7 g Na 2 CO 3 and NaH 2 PO 4 with molar ratio of 1:2; 60 mg [C 4 (MIM) 2 ]Br 2 ; 20 mg NiFe 2 O 4 ; 45 mg Na[N(CN) 2 ] and 500 μL toluene as elution solvent. The effervescent reaction-enhanced microextraction was combined with high performance liquid chromatography and fluorescence detection, rendering limits of detection as low as 0.01–0.07 μg kg −1 with high recoveries (82.3–104.7%) for five PAHs in raw, boiled, charcoal-grilled and smoked meats. To the best of our knowledge, this is the first report on the additive action between DILs and NiFe 2 O 4 in an effervescent reaction-enhanced microextraction, showing great analytical potential in meat matrices.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Juan Wang,Zhiyan Chen,Zhiming Li,Yaling Yang
A simple and rapid magnetic nanoparticles (MNPs) based dispersive micro-solid-phase extraction (D-μ-SPE) method coupled with HPLC-DAD has been proposed for simultaneous determination of three estrogens (17β-estradiol (E2), estrone (E1) and diethylstilbestrol (DES)) in pork samples. In this paper, the synthesis of cetyltrimethyl ammonium bromide (CTAB)-coated Fe 3 O 4 @caprylic acid NPs as an efficient sorbent for its high surface area, excellent adsorption capacity, good dispersion ability and high super-paramagnetic property was successfully applied to adsorb estrogens. Vortex was used to enhance mass transfer rate as it provided mild and effective mixing of sample solution and increased the contact between analytes and MNPs. The parameters affecting the extraction efficiency were investigated in detail. The dosages of sorbent and eluate are 100 μL and 500 μL, respectively. The extraction equilibrium was achieved within 2 min and the MNPs can be reused. The proposed technique provided high recoveries (93.3–106.7%), good linearity (0.9993–0.9999), low LODs (0.021–0.033 ng mL −1 ) and repeatability (RSD% = 1.87–2.92).
研究领域:
分析化学
·
食品安全
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）