Arabian Journal of Chemistry
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2023
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IF 6
Bushra Rafique,Rizwan Ullah Khan,Aysha Sarfraz Rizvi,Muhammad Irfan,Ghulam Murtaza,Lili Qiu,Min Xue,Zihui Meng
There is a demand for simple, selective, and efficient assays for the determination of clinically important metabolites such as creatinine for healthcare. Creatinine is the by-product of muscle energy metabolism and is excreted by the kidneys. To measure creatinine in the human serum, a creatinine imprinted photonic crystal hydrogel (CIPC hydrogel) for naked-eye detection is developed. CIPC hydrogel utilizes polystyrene-based two-dimensional (2D) photonic crystal colloidal arrays (PCs-array) embedded in the polyacrylamide hydrogel containing methacrylic acid which imprinted the creatinine template. The nanocavities in the hydrogel produced after the removal of the template bind to and recognize creatinine in the serum samples. The binding is selective and specific for creatinine. The binding is observed as shrinkage of the hydrogel volume and a decrease in the particle spacing which is monitored through changes in the Debye diffraction ring diameter and a visible blue-green to blue color shift. The binding event and the mechanism are investigated by molecular dockings. The CIPC sensor demonstrates a limit of detection (LOD) of 2.45 ± 1.6 µM, a linear detection range (25–500 µM), and recovery from 85.6 % to 99.9 % in the serum samples. CIPC hydrogel is available for the rapid and quantitative onsite detection of creatinine in the human serum sample.
研究领域:
生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
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2020
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IF 5.339
Shi-En Chen,Jingwen Hu,Ping Yan,Jing Sun,Wenhui Jia,Shuyun Zhu,Xian-En Zhao,Huwei Liu
Urinary sarcosine was considered to be a potential biomarker for prostate cancer (Pca). In this work, an integrated strategy of multiplex tags chemical isotope labeling (MTCIL) combined with magnetic dispersive solid phase extraction (MDSPE), was proposed for specific extraction and high-throughput determination of sarcosine by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). In the past three months, we have developed 8-plex MTCIL reagents with excellent qualitative and quantitative performance. In this work, the multiplexing capacity of MTCIL reagents (MTCIL360/361/362/363/364/365/366/375/376/378/379/381) was increased 1.5-fold from 8-plex to 12-plex. MTCIL359 was prepared and used to label sarcosine standard as internal standard (IS). The structural analogue derivative (MTCIL373-sarcosine) of all targeted MTCIL-sarcosine derivatives was synthesized and used as a novel dummy template to prepare dummy magnetic molecularly imprinted polymers (DMMIPs). The integration of MTCIL and DMMIPs procedures were extremely favorable to excellent chromatographic separation and efficient mass spectrometric detection. The labeling efficiency, chromatographic retention and mass spectrometry responses of MTCIL reagents were consistent for sarcosine. In a single UHPLC-MS/MS run (2.0 min), this method can simultaneously quantify sarcosine in 12-plex urine samples and achieve unbiased concentrations comparison between different urine samples. Analytical parameters including linearity (R 2 0.989–0.997), detection limits (0.02 nM), precision (2.6–11.5%), accuracy (96.1–107.4%), matrix effect, labeling and extraction efficiency were carefully validated. The proposed method was successfully applied for urinary sarcosine determination of healthy male individuals and Pca patients. It was found that the sarcosine concentrations in these two groups were statistically extremely significantly different ( P < 0.001). The developed method was a powerful analytical tool to substantially promote the analysis throughput and large-scale experiments about the potential biomarker research.
研究领域:
分析化学
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医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ADVANCED POWDER TECHNOLOGY
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2024
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IF 4.2
Han Hao,Tiantian Ren,Cuilu Quan,Wei Guo,Jing Wang
Pharmaceutical cocrystal is an emerging strategy not only to improve physicochemical properties, but also to generate synergistic effects. In this work, a novel cocrystal composed by 5-fluorouracil (5-FU) and sarcosine (SAR) was successfully assembled by wet powder grinding method. The cocrystal was characterized by single crystal X-ray diffraction (SCXRD), powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and Raman spectra. The differences of 1 H NMR spectroscopy between 5-FU/SAR physical mixture (PM) and cocrystal hinted the existence of supramolecular interactions between 5-FU and SAR in solid and solution. Bioavailability of cocrystal was increased than 5-FU. Most importantly, the weak interactions between 5-FU and SAR in cocrystal solution induced the superior cellar inhibition activity on 4T1 and BEL-7402 cells compared than 5-FU and PM. Cocrystal can interfere with the stability of methionine cycle and tetrahydrofolate (FH 4 ) level through the SAR-Gly-Met pathway, enhance the inhibition of thymidylate synthase (TS) compared to 5-FU, thereby interfering with cell cycle and inducing cell apoptosis. The results provided the novel strategy to develop the cocrystal drugs using bioactive amino acids as precursors to enhance the efficacy.
研究领域:
药学
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肿瘤学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
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2023
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IF 6.6
Xiaofang Zhang,Wenhuang Xiao,Shenghui Xie,Gaochao Fan,Xinlin Shi,He Meng,Haipeng Yang
Hydrogen peroxide (H 2 O 2 ) is an important signaling molecule that regulates many biological processes. It is of great value to realize rapid, accurate, highly sensitive and low-cost H 2 O 2 detection in the immediate detection of trace disease markers. Low-density nanoelectrode arrays have a high signal-to-noise ratio and are advantageous for trace detection. In this paper, low-density Pt nanoelectrode arrays were successfully prepared by a simple method of ion sputtering combined with electrodeposition based on a track-etched polycarbonate membrane template. The arrays had a detection limit as low as 0.03 μM for H 2 O 2 at a constant potential, a sensitivity of 125.9 μA mM −1 , and a linear range spanning nearly four orders of magnitude (0.1–655 μM). The developed sarcosine sensors based on these arrays had a very low detection limit (0.08 μM) and wide linear range (0.5–64.5 μM) for the rapid detection of trace amounts of sarcosine in serum. With a wide linear range and low detection limits, this hydrogen peroxide sensing platform has great potential for use in the rapid electrochemical detection of trace substances.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2021
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IF 13.273
Zhiqiang Ma,Lijun Yang,Yutong Wang,Mengfan Wang,Wei Qi,Zhimin He
Peptide assembly provides a new approach for the construction of enzyme-like catalysts. However, low stability is still a big challenge for the peptide-based enzyme mimics because the non-covalently driven structures are often destroyed when exposed to environmental changes. Inspired by the catalytic centre of natural horseradish peroxidase (HRP) and the biological function of dityrosine in structural proteins, in this study, we presented the construction of a stable peptide-based HRP mimic (CCA-YH) through introducing covalent bonds . CCA-YH was obtained through the co-assembly of YYHYY and hemin, followed by a ruthenium-mediated photo-crosslinking reaction. Catalytic activity assay demonstrated that CCA-YH displayed enhanced catalytic ability than the non-covalent co-assemblies (CA-YH). Remarkably, CCA-YH exhibited superior long-term stability than CA-YH and natural HRP under different pH, temperature, ion strength and organic medium environments, as well as a favorable reusability. Finally, CCA-YH was applied to the colorimetric detection of uric acid and sarcosine in human urine, which displayed satisfactory sensitivity, specificity and reproducibility. This is the first report the construction of stable peptide-based enzyme mimic based on covalent co-assembly strategy.
研究领域:
生物化学
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分析化学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Hanyu Wang,Yanting Qi,Dan Wu,Qin Wei
In this study, lead(II) sulfide (PbS) nanocrystals were modified on nickel(II)oxide nanosheets (NiO NSs) via the chemical bath method. Afterwards, Au nanoparticles (NPs) were also modified successfully. A photoelectrochemical (PEC) self-powered platform for detecting sarcosine with high PEC activity was constructed. The capacity of NiO NSs to be loaded with other sensitizing materials was mainly attributed to its porous structure and large specific surface area. Under optimum conditions, the constructed PEC self-powered cathodic sensor for detecting sarcosine exhibited a linear range in 5.0 × 10 −8 –5.0 × 10 −2 mol/L with a detection limit (LOD) of 1.7 × 10 −8 mol/L. The biosensor demonstrated good reproducibility, acceptable stability and high specificity, thus confirming its potential application in the detection of other similar substances.
研究领域:
分析化学
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材料科学
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生物传感器
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电化学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Ya Jiang,Qingqing Jiang,Daming Fan,Mingfu Wang,Yueliang Zhao
The effect of acrolein, a lipid oxidation product, on the formation of the heterocyclic aromatic amine 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) was investigated in a chemical model containing glycine, creatinine, and glucose. Acrolein addition at 0.02–0.2 mmol increased MeIQx formation, while high levels of acrolein (>0.2 mmol) did not further increase MeIQx formation. Moreover, acrolein addition decreased the residue of glycine and creatinine but increased the residue of glucose; it also increased the formation of volatile intermediates in the MeIQx-producing chemical model. Further analysis indicated that acrolein can react with glycine, creatinine, and MeIQx to eliminate them. These results revealed that acrolein was able to contribute to MeIQx formation as a consequence of the comprehensive ability of acrolein to facilitate Strecker degradation of glycine, increase the formation of volatile intermediates, and react with glycine, creatinine, and MeIQx. In addition, the oxidation of minced salmon increased the content of MeIQx in the roasted salmon patties, further supporting the potential contribution role of lipid oxidation products in the formation of MeIQx.
研究领域:
食品科学
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化学
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毒理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）