FOOD CHEMISTRY
·
2025
·
IF 9.8
Hongxia Feng,Wenhui Ye,Wenqin Wang,Mingfa Song,Juncai Hou,Yunhe Chang
Idesia polycarpa ( I. polycarpa ) oil by-products are rich in phospholipids (PLs), yet research on their composition and functionality remains limited. This study compared six degumming methods-hydration degumming (HD), acid degumming (AD), phospholipase A 1 degumming (PLA 1 D), simple phospholipase C degumming (SPLCD), deep phospholipase C degumming (DPLCD), and compound degumming (CD) –to evaluate their effects on PL characteristics. The results showed that enzymatic degumming methods, particularly CD, achieved the highest degumming rate of 94.7 %, significantly outperforming non-enzymatic methods. Lipidomic profiling revealed that all treatments resulted in phospholipids enriched with polyunsaturated fatty acids (PUFAs), especially linoleic acid (C18:2). Enzymatic treatments produced PLs enriched in lysophosphatides, while non-enzymatic methods retained mostly intact phospholipids. These findings suggest that I. polycarpa oil by-products, particularly those treated by enzymatic degumming, can be a valuable and sustainable source of PUFA-rich phospholipids, with potential applications in the food, pharmaceutical, and cosmetic industries.
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PubMed
高影响因子
Environmental Technology & Innovation
·
2024
·
IF 6.7
Mengyao Pan,Yuwei Jin,Yanli Ye,Wenbin Jiang,Lisha Zhu,Wangyang Lu
Silk is a promising textile resource, but its degumming treatment still lacks efficient and non-polluting methods. In this paper, an innovative silk degumming process combining microwave pre-treatment and steam degumming is introduced. When the technical parameters of microwave-steam synergistic degumming were 10 min of microwave, 120 ℃ of steam temperature, and 30 min of steam time, the degumming efficiency of the specimen was three times of the traditional Na 2 CO 3 degumming method, and the energy consumption was only one-half of the traditional method. In terms of time efficiency, the traditional Na 2 CO 3 degumming took 2.75 h, while this novel method took only 0.75 h. Among the samples, the mechanical properties of the specimens treated with microwave for 10 min were the best, with the warp breaking strength of 542.20 N and the weft breaking strength of 356.19 N. Meanwhile, the hygroscopicity and air permeability were also the best compared to the traditional treating samples. This indicates that microwave-steam synergistic degumming technology is a clean technology with the advantages of high sericin degumming rate, short degumming time, low energy consumption, and no use of any chemicals. This method is conducive to achieving better and faster sustainable development.
研究领域:
纺织科学
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环境科学
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材料科学
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
·
IF 4.6
Li Zhou,Tianyu Zhang,Timothy J. Tse,Martin J.T. Reaney,Siya Zhao,Zilun Cao,Shu Wang,Zhigang Hu,Bin Li,Chuanrong Hu,Dongping He,Fenfen Lei,Qinfeng Zhang
Degumming, a critical process in the edible oil industry, is essential for removing phospholipids. Traditional methods such as water-degumming and acid-degumming have limitations in this regard. This study aimed to assess the efficacy of different degumming techniques, including acid, single and multi-enzyme methods, on rice bran oil (RBO). The investigation also focused on the impact of these techniques on the physicochemical characteristics and preservation of micronutrients in RBO during the degumming process. The primary phospholipids identified were phosphatidylethanolamine (29.51%), phosphatidylcholine (37.00%), and phosphatidylinositol (24.49%). Acid degumming removed 84.23% of phospholipids, while a significantly higher removal rate of 98.7% was achieved with the combination of phospholipase A1 & phospholipase C. The degumming process effectively inhibited oxidation in RBO, leading to a substantial increase in the oxidation induction time from 5.7 to 10.0 hours. Furthermore, multi-enzyme degumming showed slightly greater radical-scavenging activity compared to single enzyme degumming in RBO. However, the levels of micronutrients such as phenols, sterols, tocopherols, squalene, and oryzanol were reduced by 6.27% to 22.17%. This study provides a comprehensive analysis of the effects of different degumming processes on the physicochemical properties, fatty acid profiles, antioxidant capacities, and preservation of micronutrients in RBO.
研究领域:
食品科学
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生物化学
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高影响因子
Polymers
·
2023
·Vol. 15
·期 16
·3383
·
IF 5
Xueping Liu,Qian Huang,Peng Pan,Mengqi Fang,Yadong Zhang,Shanlong Yang,Mingzhong Li,Yu Liu
Removing sericin from the periphery of silk without damage to silk fibroin (SF) to obtain high-molecular-weight SF is a major challenge in the field of SF-based biomaterials. In this study, four neutral proteases, subtilisin, trypsin, bromelain and papain, were used to degum silk, and the degumming efficiency of the proteases and their influence on the molecular weight (MW) of regenerated silk fibroin were studied. The results indicated that all four neutral proteases could remove sericin from silk almost completely, and they caused less damage to SF fibers than Na2CO3degumming did. The degumming efficiency of trypsin and papain was strong, but they caused relatively high damage to SF, whereas bromelain caused the least damage. The results of sodium dodecyl sulfate–polyacrylamide gel electrophoresis, gel permeation chromatography and shear viscosity showed that the MWs of regenerated SF derived from neutral protease degumming were significantly higher than that of SF derived from Na2CO3degumming. The MW of regenerated SF derived from bromelain degumming was the highest, while the MWs of regenerated SF derived from papain and trypsin degumming were relatively low. This study provides an efficient and environmentally friendly biological degumming method for obtaining high-molecular-weight silk fibroin.Keywords:silk fibroin;degumming;neutral protease;molecular weight
研究领域:
生物材料学
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蛋白质工程
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jiaojiao Chen,Yiwei Zhang,Mei Zhao,Xinyi Zan,Xiaohong Pan,Cunsheng Zhang,Ziwei Chen,Hossain M. Zabed,Xianghui Qi
Pectate lyase (PL) holds significant potential for applications in various industries. However, the existing PL was unable to adapt to thermo-alkaline industrial environments. In this work, a PL from Caldicellulosiruptor bescii DSM 6725 (hereafter, CbPelD) was studied to disclose its structural and biochemical properties. CbPelD consisted of a putative signal peptide sequence, a short linker region, and a catalytic domain (PelD-II), along with an exoacting β-fructofuranosidase domain (PelD-I). In molecular dynamics simulation, PelD-II demonstrated significantly higher activity and thermal stability compared to other truncated enzymes of CbPelD. The optimal pH and temperature for PelD-II were 10.0 and 60 °C, respectively, with the specific activity increasing by 164–223% in the presence of Ca2+ or Ni2+. The exotype PelD-II exhibited efficient degradation of polygalacturonic acid (PGA) into unsaturated galacturonic acid (uG1), digalacturonic acid (uG2), and trigalacturonic acid (uG3). In the bioscouring assay, PelD-II exhibited a significant increase in the wetted fabric area to 6.072 ± 0.684 cm2, which was about 7.9 times higher than untreated fabric. Scanning electron microscopy further revealed that treatment with PelD-II resulted in a smoother fiber surface, providing direct visual confirmation of the enzyme’s action. These results highlight the potential of PelD-II for applications in the textile industries.
研究领域:
酶学
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生物技术
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纺织工业
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Xuan Liu,Weifei Wang,Long Xu,Zhong Li,Qingqing Xu,Bo Yang,Dongming Lan,Yonghua Wang
Phospholipids (PLs), as natural additives and nutritional supplements, are recovered from the gum phase during the degumming process of crude vegetable oils. The molecular species of phospholipids recovered from crude soybean (SO), flaxseed (FO) and peanut oil (PO) by different degumming processes, including water (WD), acid (AD), PLA 1 (EDA), PLC (EDC) and PLA 1 -PLC (EDAC) were investigated. According to the PLS-DA model, a total of 30, 38 and 21 characteristic markers were identified using UPLC-Q-TOF-MS in the phospholipids recovered from SO, FO and PO by different degumming processes, respectively. PC (18:2/18:2) and PE (18:2/18:2) were the main phospholipids for WD and AD, LPC (18:2) and LPE (18:2) were the main phospholipids for EDA and EDAC, and PI (16:0/18:2) and PI (16:0/18:1) were served as characteristic phospholipids for EDC. In addition, a lamellar liquid-crystalline phase was observed in the recovered phospholipids, which indicated that the microstructure of phospholipids recovered from crude vegetable oils were significantly influenced by different degumming processes. These differential molecular species of phospholipid products provide useful information to facilitate the potential utilization of phospholipid products as emulsifiers in food and as ingredients in the pharmaceutical industries.
研究领域:
食品科学
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生物化学
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高影响因子
Journal of Natural Fibers
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2023
·
IF 3.5
Boya Li,Kai Nie,Linlin Li,Gang Fu,Chunguo Liu,Shouwu Gao,Guangting Han,Tao Zhao,Wei Jiang
The Sesamum indicum bast contains more hemicellulose, lignin, and flaky parenchymal cells than the commonly used ramie bast, which are rigid for the degumming process. We explored a facile and greener method by using glycolic acid microwave-assisted treatment combined with alkaline treatment to effectively and low-cost prepare the high-quality Sesamum indicum single fibers. The chemical and physical properties of fibers were analyzed by chemical composition analysis, SEM, FTIR, XRD, TG, and GPC analysis. The residual gum content was decreased from 34.4% to 4.2%, resulting in a cleaner micro-surface of the Sesamum indicum fibers with increased cellulose crystallinity (75.2%–82.5%) and thermal stability (357°C–365°C). The fiber fineness (2.5 dtex) and breaking tenacity (4.9 cN/dtex) of the fibers produced by the novel treatment method exhibited the same level compared with the traditional two-step alkaline fibers. Besides, both the consumption of water and the chemicals was decreased compared with traditional degumming. This work provided a novel green and facile degumming method for processing the natural bast fibers.
研究领域:
材料科学
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植物学
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高影响因子
Journal of Natural Fibers
·
2022
·
IF 3.507
Yinzhi Yang,Yuqing Liu,Yuxi Fang,Yanfei Teng,Kaiyang Fang,Jinpeng Xie,Xinwang Cao,Zhongmin Deng
The bamboo shoot shell fibers were extracted by using environmental protection oxidant sodium percarbonate combined with alkali oxygen bath method. To explore the influence of various factors on its degumming, the efficiency of degumming through single-factor and orthogonal experiments was assessed. The results showed that the used method had a good effect on the degumming of bamboo shoot shell fiber, and most of the non-cellulose components such as hemicellulose, lignin, and pectin had been removed. After the treatment, the cellulose content and crystallinity were 73.19% and 61.40%, respectively. Taking the degumming rate as the index, the factors such as dosage of sodium percarbonate and hydrogen peroxide, soaking temperature, and time in alkali boiling treatment were optimized. The results showed that the degumming rate was 69.17% when the scouring temperature was 90°C, the alkali boiling time was 120 min, the dosage of sodium percarbonate was 24 g/L, and the dosage of hydrogen peroxide was 40 mL/L. The structure and properties of bamboo shoot shell fiber were analyzed by scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analyzer (TGA). The prepared bamboo shoot shell fibers would be considered as a promising and sustainable raw material for the textile industry and other prospects in the future.
研究领域:
材料科学
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纺织工程
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高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 13
·8375-8386
·
IF 5.044
Fan Fangwei,Zhu Mengting,Fang Kaiyang,Xie Jinpeng,Deng Zhongmin,Wang Xianfeng,Zhang Zhen,Cao Xinwang
This paper reports an improved traditional fiber degumming method, where sisal fibers were treated by alkali oxygen and pectinase, respectively, after the solute alkali pretreatment. To explore the influence of various factors on its degumming, efficiency of degumming through single factor and orthogonal experiments was aasessed. The results showed that pectinase/alkali-oxygen method after the first alkali treatment had a good effect on the degumming of sisal fiber, and most of the non-cellulose components such as hemicellulose, lignin and pectin had been removed. After pectinase treatment, the cellulose content and crystallinity were 71.87% and 66.29%, respectively. After alkaline oxygen treatment, the cellulose content was 77.16%, and the crystallinity was 69.09%. In terms of degumming rate, alkali oxygen treatment worked better than pectinase treatment, the degumming rate of pectinase method was about 10%, while that of alkali-oxygen method was more than 20%. In other hand, the pectinase method was much milder and had less damage to fibers. It would provide some references for the future application and development of sisal fiber.
研究领域:
生物化学
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材料科学
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhong Li,Huilin Zhou,Xuan Liu,Weifei Wang,Dongming Lan,Yonghua Wang
The limited availability of phospholipase A 1 (PLA 1 ) has posed significant challenges in enzymatic degumming. In this study, a novel PLA1 (UM2) was introduced to address this limitation, which had a unique thermo-responsive ability to switch phospholipase and lipase activities in response to temperature variations. Remarkably, UM2 displayed an unprecedented selectivity under optimized conditions, preferentially hydrolyzing phospholipids over triacylglycerols—a specificity superior to that of commercial PLA 1 . Moreover, UM2 demonstrated high efficiency in hydrolyzing phospholipids with a predilection for phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A practical application of UM2 on crude flaxseed oil led to a dramatic reduction in phosphorus content, plummeting from an initial 384.06 mg/kg to 4.38 mg/kg. Broadening its industrial applicability, UM2 effectively performed enzymatic degumming for other distinct crude vegetable oils with a unique phospholipid composition. Collectively, these results highlighted the promising application of UM2 in the field of oil degumming.
研究领域:
生物化学
·
食品科学
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DOI
PubMed
高影响因子
TEXTILE RESEARCH JOURNAL
·
2018
·
IF 1.54
Xiang Yeping,Yu Jianyong,Liu Liu,Zhang Ruiyun,Qu Yongshuai,Jing Miaolei
In this study, we found an environmentally friendly system in the degumming of hemp fiber without using a strong acid and alkali. The chemo-enzymatic modification and degumming of hemp fiber used an acetic acid–sodium acetate buffer solution containing laccase, hemicellulase and 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) under a mild condition (pH = 5, T = 50℃). Through comparing and analyzing the physical and chemical properties of fiber products under different treatments, we found the method involving treatment with TEMPO/laccase/hemicellulase not only greatly removed most of the gum attached on the surface of the raw hemp fiber, but also introduced surface-active aldehyde and carboxyl groups into the fiber. The performances of the hemp fibers with different treatments were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. We also found the product was finer, with a lower content of non-cellulosic components and it had better water uptake properties (moisture sorption and water retention value), which has potential application values in textile industries.
研究领域:
材料科学
·
生物技术
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xuan Liu,Weifei Wang,Zexin Zhao,Long Xu,Bo Yang,Dongming Lan,Yonghua Wang
Enzymatic degumming is an essential refining process to improve oil quality. In this study, a monoacylglycerol lipase GMGL was derived from marine Geobacillus sp., and was found that not only took monoacylglycerol (MAG) as substrate, but also had activity toward lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE) and glycerolphosphatidylcholine (GPC). Binding free energy showed LPC and LPE could bind with enzyme stably as MAG. It presented great potential in the field of enzymatic degumming. The phosphorus content in crude soybean oil decreased from 680.50 to 2.01 mg/kg and the yield of oil reached to 98.80 % after treating with phospholipase A1 (Lecitase Ultra) combined with lipase GMGL. An ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was developed to identify 21 differential phospholipids between crude soybean oil and enzymatic treatment. This work might shed some light on understanding the catalytic mechanism of monoacylglycerol lipase and provide an effective strategy for enzymatic degumming.
研究领域:
食品科学
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酶学
·
脂类化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）