Journal of Pharmaceutical Analysis
·
2019
·
IF 4.44
Dongsheng Xu,Elena Sánchez-López,Qiqin Wang,Zhengjin Jiang,María Luisa Marina
An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mobile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolution performance. 27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested, and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of them. Analytical characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity, precision, accuracy, limits of detection (LOD) and quantitation (LOQ) showing good performance to be applied to the enantiomeric determination of these amino acids in dietary supplements. LOD and LOQ values were 9.3 and 31 μM for norvaline enantiomers and 7.5 and 25 μM for tryptophan enantiomers, respectively. The contents of d -norvaline and d -tryptophan were below their respective LODs in all the analyzed samples. Quantitation of l -tryptophan and l -norvaline showed good agreement with the labeled contents except for one sample which did not show presence of l -norvaline, contrary to the label indication.
研究领域:
分析化学
·
生物化学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Yongmei Wang,Zongshan Zhao,Jianyuan Qin,Huan Liu,Aifeng Liu,Mengmeng Xu
A simple, green and nondestructive method based on terahertz fingerprint peaks has been developed for rapid in situ analysis of l -histidine and α-lactose in dietary supplements. Fingerprint absorption peaks of l -histidine and α-lactose located at 0.77 and 0.53 THz could be directly used for identification and quantitation of these analytes in commercial dietary supplements. Compared with the partial least squares regression model (PLSR), the linear least squares regression (LLSR) method based on peak areas presented better performance, with the linear correlation coefficients of 0.9899 and 0.9910 for l -histidine and α-lactose, respectively. Furthermore, analysis time per sample can be shortened to less than 1 min due to the narrower spectral acquisition region. The accuracies were 94.8–110% and 98.9–110%, comparable to those of ion chromatography for l -histidine and high-performance liquid chromatography for α-lactose. The results presented great potential of the developed method for rapid in situ analysis of nutrients in dietary supplements.
研究领域:
分析化学
·
营养学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 21
·2685-2692
·
IF 3.1
Huanan Huang,Yihui Chen,Meilin Ni,Jing Du,Chunyan Hou,Xiaoqiang Qiao,Guozhou Cao,Tingting Wang
Raw halloysite was purified by using sodium hexametaphosphate and utilized as the solid-phase extraction sorbent for the determination of biguanides from dietary supplements. The purified halloysite was characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The purified halloysite interacted with biguanides through hydrophilic interaction and ion exchange on account of its abundant hydroxyl groups and negative charge. Compared with traditional extraction methods based on hydrophobic interaction and/or ion exchange, the purified halloysite adsorbed more biguanides due to hydrophilicity and ion exchange, with a sample loading volume of up to 100 mL at least. Excellent reproducibility of halloysite purification was achieved, with within-batch (n = 3) and batch-to-batch (n = 3) relative standard deviations in the ranges of 1.5–4.2% and 5.6–8.8%, respectively. Coupled with reversed-phase liquid chromatography-tandem mass spectrometry, a low limit of detection of 0.3 μg kg−1 was obtained. The intra- and inter-day mean recoveries of the biguanides spiked at three levels in dietary supplements were within the ranges of 88.5–107.2% and 86.4–102.0%, respectively. The intra- and inter-day precisions were within the ranges of 1.5–6.4% and 5.4–9.9%, respectively. These results indicated that the developed method is efficient for the determination of trace biguanides in dietary supplements.
研究领域:
分析化学
·
药物分析
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Hongyan Li,Yiying Fu,Wangbao Gong,Guangjun Wang,Zhifei Li,Jingjing Tian,Kai Zhang,Ermeng Yu,Yun Xia,Wenping Xie,Jun Xie
The pregnane X receptor (PXR) / nuclear factor-kB (NF-kB) signaling pathway plays a critical role in regulating toxin-induced inflammation and apoptosis in mammals. Whether dietary curcumin (CUR) can prevent copper (Cu)-induced liver injury via this signaling pathway remains to be established in aquatic animals. Juvenile largemouth bass ( Micropterus salmoides ) were exposed to dietary Cu and CUR treatments for 8 weeks. The results showed that chronic Cu exposure induced oxidative stress, causing liver function damage and liver injury. Cu exposure stimulated inflammation by regulating nf-kb and pro-inflammatory genes such as tnfα and il-1β and promoted apoptotic signals in the liver by modulating bcl2 and casp3 mRNA levels. In addition, Pearson correlation analysis verified that inflammatory and apoptotic responses were important indicators of Cu-induced liver injury. CUR attenuated stress responses by enhancing the antioxidant system. Importantly, CUR significantly stimulated PXR mRNA and protein levels in the Cu + CUR group and suppressed NF-κB activation to inhibit the inflammatory and apoptotic signaling cascade. These results suggest that CUR may be an effective activator of PXR in teleost fishes, exerting cytoprotective effects on Cu-induced liver injury via a PXR-mediated NF-κB repression mechanism. In conclusion, this study is the first to demonstrate that CUR may act as a potent PXR ligand that exerts hepatoprotective effects against Cu-induced liver injury. The findings shed light on the specific regulatory role of the PXR/NF-κB signaling pathway in liver pathogenesis and its potential as a therapeutic target in teleost fishes.
研究领域:
水生毒理学
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分子生物学
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营养学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2020
·
IF 3.004
Shi Shi,Yuanyang Wu,Minghao Zhou,Qiaoyuan Cheng
In this paper, an ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF HRMS) method was developed and validated for screening, confirmation and quantitation of 31 anti-impotence compounds potentially illegally added to herbal-based dietary supplements. The analytes were well separated by the mobile phase consisted of 0.1% formic acid solution and acetonitrile with gradient elution at a flow rate of 0.3 mL/min. The MS analysis was operated in positive mode and the mass error of the 31 compounds were below 2.9 ppm. The method validation showed good linearity with coefficients of determination (r 2 ) higher than 0.9973 for all analytes. LODs and LLOQs ranged from 0.005 to 0.50 μg/g or μg /mL and from 0.02 to 1.24 μg /g or μg/mL, respectively. The accuracy was in the range of 86.6% to 113.7%, while the intra-and inter-day precision were in the ranges of 0.9–7.6% and 0.9–11.4%, respectively. The absolute and relative matrix effect were in the range of 65.8–115.6% and 0.6–13.3%. The mean recoveries were in the range of 80.5–116.9%. The stability ranged from 0.4% to 8.5%. Among 200 batches of herbal-based dietary supplements, sildenafil and/or tadalafil were found to be added illegally in two samples, while not very high concentration of icariin was detected in one sample. The Q-TOF mass spectrometry has been proved to be a very powerful and efficient tool for rapid screening of 31 anti-impotence compounds potentially illegally added to herbal-based dietary supplements, ensuring food safety and public health.
研究领域:
分析化学
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食品安全
·
公共卫生
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Qiaoyuan Cheng,Linjun Shou,Cen Chen,Shi Shi,Minghao Zhou
In this paper, an ultra-high-performance liquid chromatography coupled with linear ion trap quadrupole Orbitrap high resolution mass spectrometry (UHPLC-LTQ-Orbitrap HRMS) method was developed and validated for identification, confirmation and quantitation of illegal adulterated weight-loss drugs in plant dietary supplements. 13 wt-loss drugs were well separated by the gradient elution of 10 mmol/L ammonium acetate − 0.05% formic acid H 2 O and acetonitrile at a flow rate of 0.2 mL/min within 12 min. The MS analysis was operated under the positive ion and in full MS/dd-MS 2 (data-dependent MS 2 ) mode. The full MS scan with resolution at 60 000 FWHM and narrow mass windows at 5 ppm acquired data for identification and quantitation, and dd-MS 2 scan with resolution at 15 000 FWHM obtained product ions for confirmation. The method validation showed good linearity with coefficients of determination (r 2 ) higher than 0.9951 for all analytes. Meantime, all the LOD and LLOQ values were in the respective range of 0.3–2 and 1–9 ng/g. The accuracy, intra- and inter-day precision were in the ranges of −1.7 to 3.4%, 1.7–5.0% and 1.9–4.4%, respectively. The mean recoveries ranged from 85.4 to 107.1%, while the absolute and relative matrix effect were in the corresponding range of 98.2–108.6% and 2.6–8.7%. Among 120 batches of weight loss plant dietary supplements, sibutramine and fluoxertine or both were positive in 29 samples. In general, LTQ-Orbitrap HRMS technology was a powerful tool for the analysis of illegal ingredients in dietary supplements.
研究领域:
分析化学
·
药学
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DOI
PubMed
高影响因子
STARCH-STARKE
·
2022
·
IF 2.688
Mengyu Zhang,Wenshuai Wang,Wenxue Sun,Jianjun Zhang,Le Jia
This study investigates the efficacy of chewable tablets made from the Pleurotus djamor zinc polysaccharide (PCT-Zn) against the nonylphenol (NPs) induced male reproductive damage in mice. The trial takes the form of constructing a model by infusing NPs into mice, followed by treatment with PCT-Zn solution infusion 1 h later, for a total of 8 weeks. It is found that PCT-Zn shows potential effects on increasing the testicular coefficients, enhancing the antioxidant activities, and inhibiting the apoptotic and inflammatory responses, which indicate that PCT-Zn has the effect of alleviating the NPs-induced reproductive damage. The results of the experiment suggest that the P. djamor zinc polysaccharide can be used as a dietary supplement to develop appropriate products to alleviate reproductive damage caused by environmental pollutants.
研究领域:
环境毒理学
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生殖生物学
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药理学
·
营养科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Changyan Dong,Yi Cheng,Meihui Zhang,Mianhua Chen,Zhongli Yan,Sumei Zhou,BenxuYang,Qingbin Guo,Changlu Wang,Shufen Wu
In this study, four Monascus pigments (ankaflavin, AK; monascin MS; rubropunctatin, O 1 ; monascorubrin, O 2 ) were proved to exhibit considerable anti-glycation properties in bovine serum albumin (BSA)-fructose model. AK (40.62 %) and MS (48.38 %) were found to exert lower inhibitory effects on the formation of fluorescent advanced glycation end products (AGEs) than aminoguanidine (59.4 %), while O 1 (90.64 %) and O 2 (93.82 %) displayed much stronger abilities. AK and MS could trap methylglyoxal (MGO) with maximum capture rates of 85.67 % and 84.90 %, respectively, and only mono-MGO adducts of them were detected. LC-Orbitrap MS/MS analysis revealed that four pigments significantly altered the type and reduced the number of the glycated sites and they all covalently bound to BSA, with O 1 and O 2 possessing high reactivity. Altogether, AK and MS suppressed fluorescent AGEs formation mainly via trapping MGO and covalently interacting with BSA, and blocking free amino groups was the dominant mechanism for O 1 and O 2 . These findings presented new insights into Monascus pigments as dietary supplement for inhibiting protein glycation.
研究领域:
生物化学
·
食品科学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Tian Wei,Yongyi Zhang,Jianxin Huang,Jinyi Yang,Yuanxin Tian,Yudong Shen
In recent years, phosphodiesterase type 5 enzyme inhibitors, including tadalafil, sildenafil, and their analogues have emerged as illegal ingredients in dietary supplements. It can cause serious clinical side effects in consumers, resulting in diverse symptoms. To rapidly screen illegal drugs in dietary supplements, a dual-labeled probe time-resolved fluorescence immunochromatography assay (DL-TRFICA) based on two broad-spectrum antibodies labeled with time-resolved fluorescent microspheres was established for the simultaneous qualitative and quantitative detection of nine sildenafil-like and three tadalafil-like adulterants. The half-maximal inhibitory concentration (IC 50 ) of sildenafil-like and tadalafil-like adulterants were 2.49–5.88 and 2.15–2.78 ng mL −1 , the limit of detection were 0.08–1.66 and 0.59–1.85 ng mL −1 , and the visualization value (cut-off value) were 31.25–250 and 50–62.50 ng mL −1 , respectively. Notably, the potential practical applications of DL-TRFICA evaluated by conducting a quantitative evaluation of sildenafil and tadalafil adulterants in dietary supplement samples (herbal spirit drinks, healthcare oral liquids, and herbal medicine pills) demonstrated a recovery rate ranging from 80.0% to 125.8% with a coefficient of variation of less than 15%, which concluded that the DL-TRFICA approach could be used in real sample detection.
研究领域:
药物分析
·
分析化学
·
食品安全
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2019
·
IF 3.775
Tingyan Hu,Kegang Linghu,Siqi Huang,Maurizio Battino,Milen I. Georgiev,Gokhan Zengin,Defang Li,Yun Deng,Y.T. Wang,Hui Cao
Significant evidence indicated that flaxseed ( Linum usitatissimum ) possesses various positive health aspects such as reducing the risk of cancer and cardiovascular diseases. The fatty acids are considered to be responsible for these benefits of flaxseed. Herein, the in vitro effects of flaxseed extract on the growth and apoptosis of human breast cancer MCF-7 cells were investigated. The MCF-7 cells treated with flaxseed extract showed a dose-dependent decrease in cell viability. The flaxseed extract induced reactive oxygen species and the flow cytometric analysis demonstrated that flaxseed fatty acids triggered apoptosis of MCF-7 cells, which was also shown by the loss of mitochondrial membrane potential and caspase cascade reaction. Thus, the flaxseed extract regulated the growth of MCF-7 cells and induced apoptosis. Eventually, the flaxseed could be used as a dietary supplement to prevent breast cancer.
研究领域:
肿瘤学
·
营养学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2019
·Vol. 43
·期 11
·e13028
·
IF 1.358
Meiling Han,Ai Li,Ting Shen,Jiaxin Meng,Yongqin Lei,Xian Zhang,Pengyuan Liu,Linxin Gan,Lei Ao,Houhua Li
The study aims to analyze the phenolic compounds in Malus spp. and evaluate their antioxidant and pro-apoptotic effects in BGC-803 gastric cancer cells. The results showed that cyanidin-3-galactoside was the main polyphenol in Malus “Royalty” (MR), while catechin, epicatechin, and proanthocyanidin B1 and B2 contents were higher in Malus “Cinnabar” (MC) and Malus micromalus (MM) than in MR fruits. The total polyphenol content, total flavonoid content, and antioxidative properties of Malus spp. fruits followed an order of MR > MC > MM. Fruit extracts could inhibit BGC-803 cells growth and induce apoptosis, with IC 50 values of 0.47, 0.36, and 0.31 mg/ml for MR, MC, and MM, respectively. Furthermore, fruit extracts induced cell apoptosis through increasing Bcl-2 and Bcl-xl (pro-apoptosis) expression and inhibiting Bax and Bak (anti-apoptosis) expression, thereby accelerating cell apoptosis. Taken together, these results demonstrate that red fruits (i.e., MR and MC) were more effective against cancer cells than green fruits (i.e., MM). Practical applications Fruits of Malus spp. are ≤5 cm in size, considerably smaller than apples. They are rich in various natural bioactive compounds that are often consumed as a dietary supplement or used as natural raw materials for function food. In the current study, it is comprehensively characterized profile and bioactivity of phenolic metabolites in Malus spp. fruits of different colors, and found that red fruits are more effective in reducing the free radicals and inducing cancer cells apoptosis than green fruits. These findings are valuable for food technologists and food manufacturers, especially those who produce crabapple supplement. The study investigated the molecular mechanism of how Malus spp. fruits exert anti-cancer functions. This lays a theoretical foundation for future research on developing anti-cancer function food and provides helpful guidance for fruit market management and fruit processing industry.
研究领域:
药理学
·
肿瘤学
·
食品科学
·
植物科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）