INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yachong Zhu,Yehong Chen,Chaojun Wu,Dongmei Yu,Qijun Ding,Ronggang Li
In this study, cellulose nanofibrils (CNFs) were directly prepared after autohydrolysis of eucalyptus raw materials. The effect of different autohydrolysis times on the hydrogen bond structure, micro-structure and properties of CNFs were analyzed by FTIR peak fitting, two-dimensional infrared, TGA, TEM and X-ray diffractometer. The results showed that the autohydrolysis treatments could obviously lead to the sequence of hydrogen bond changes in CNFs as follows: v (weak hydrogen bond) > v (free hydroxyl) > v (O(3)H.O(5)) > v (O(2)H.O(6)) > v (O(6)H.O(3′)), and further impact in crystallite size of CNFs. With the increase of the autohydrolysis strength, the crystallite size of CNFs on the 1 1 ¯ 0 crystal plane could be reduced by up to 26%, but the crystallite size on the 110 crystal plane and the 200 crystal plane increased by 24.5% and 22.6%, respectively. These microstructure changes finally increased the crystallinity index of CNFs, and then resulted in their high thermal stability.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Xiaohui Guo,Yingjuan Fu,Fengshan Zhang,Xiaoliang Li,Na Liu
Autohydrolysis of wood chips prior to separating lignin and cellulose is an effective method to pre-extract hemicelluloses, where the change of the structure and chemical composition in the hydrolyzed wood chips inevitably impact the subsequent separation efficiency of the remaining components. In this work, autohydrolysis of poplar wood chips under different intensity was carried out, and the relationships between the chemical/physical changes in the hydrolyzed wood chips and the accessibility of remaining components was evaluated. The results indicated that the removal of most hemicelluloses and minor lignin increased the porosity, specific surface area, and internal hydrophilicity of the wood chips, enhancing the accessibility of wood components to alkali solution as well as the diffusion of the alkali-soluble substance outward. The hydrophobic lignin or lignin-like substance accumulated on the surface of the wood chips could rapidly dissolve into the alkali solution, thus, having no adverse effect on the permeation of alkali solution into the wood chips, although they might create a barrier blocking the passage of water penetration.
研究领域:
林产化学与工艺学
·
生物质转化
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Wei Tang,Xinxing Wu,Chen Huang,Caoxing Huang,Chenhuan Lai,Qiang Yong
Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification.
研究领域:
生物化学
·
可再生资源技术
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2023
·
IF 7.4
Xinxing Wu,Zhiwei Fan,Shadrick Mwansa,Chen Huang,Qiang Yong
Exogenous ash penalties cause straw pulp residues to exhibit low autohydrolysis performance due to an organic–inorganic colloid medium. In this study, H 2 O 2 was applied following the soil organism to oxidize the organic components playing a role in the buffer system by increasing the negative charge on the surface of the particulate ash, thus reducing the acid buffer and cation exchange capacity of the pretreated wheat straw pulp residue (WSPR). The results showed that with the enzymatic efficiency increasing concentrations (0–3%) of H 2 O 2 , the enzymatic efficiencies of pretreated WSPR improved significantly with increasing H 2 O 2 concentration (0–3%) from 49.73 to 88.27%. A linear correlation was observed between these increases and the accessibility of the substrate to the enzymes, the substrate's surface charge, and the lignin's available surface area. The addition of 2% H 2 O 2 in WSPR autohydrolysis was able to increase the production of glucose and xylooligosaccharides by 1.58 and 2.38 times, respectively. This work demonstrated an innovative option for producing fermentable sugars from WSPR by effectively abolishing the self-buffering effects during autohydrolysis.
研究领域:
生物化学
·
可再生资源与环境科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Junjun Zhu,Han Zhang,Ningxin Jiao,Yuxin Xiao,Dawei Shi,Yong Xu
This study proposed a clean sequential process, including autohydrolysis, the hydrolysis of endoxylanase, and adsorption with activated carbon (AC), that assisted refining poplar ( Populus spp.) into low degree of polymerization (DP) xylooligosaccharides (XOS). First, autohydrolysis was conducted at 170 °C for 50 min. The autohydrolysate was then hydrolyzed with 1.0 International Unit (IU) per mL of endoxylanase to increase the concentration of low DP XOS (X2-X4) from 2.98 to 6.42 g/L. The recovery of XOS from the XOS rich enzymatic hydrolysis liquor reached 96.5% using 10% AC with a gradient elution of ethanol, and the highest purity of XOS was 98.2% from the 40% ethanol eluent. Finally, the separated spent liquor (SL) obtained after AC adsorption was assayed for bioactivity on rice ( Oryza sativa L.). Spent liquor revealed a positive dose-dependent bioactivity toward rice growth, which promoted seed germination, plant height, root length and fresh weight. These effects were attributed to an appropriate amount of acetic acid and XOS, which could be used as plant biostimulants.
研究领域:
生物化学
·
植物学
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产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2019
·Vol. 43
·期 1
·111-122
·
IF 2.371
Li Mingfu,Guo Chenyan,Luo Bin,Chen Changzhou,Wang Shuangfei,Min Douyong
An autohydrolysis pretreatment with different conditions was applied to sugarcane bagasse to compare the impacts of the physicochemical properties and hydrolytic inhibitors on its enzymatic hydrolysis. The results indicate that the autohydrolysis conditions significantly affected the physicochemical properties and inhibitors, which further affected the enzymatic hydrolysis. The inhibitor amount, pore size, and crystallinity degree increased with increasing autohydrolysis severity. Furthermore, the enzymatic hydrolysis was enhanced with increasing severity owing to the removal of hemicellulose and lignin. The physicochemical obstruction impeded the enzymatic hydrolysis more than the inhibitors. The multivariate correlated component regression analysis enabled an evaluation of the correlations between the physicochemical properties (and inhibitors) and enzymatic hydrolysis for the first time. According to the results, an autohydrolysis with a severity of 4.01 is an ideal pretreatment for sugarcane bagasse for sugar production.
研究领域:
生物技术
·
可再生能源
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DOI
PubMed
高影响因子
Biotechnology for Biofuels
·
2020
·Vol. 13
·期 1
·1-9
·
IF 4.815
Wu Xinxing,Tang Wei,Huang Chen,Huang Caoxing,Lai Chenhuan,Yong Qiang
Background Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.Results Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~ 84.3–61.4% to 72.3–53.0% by loading (1–30 g/L) sodium phosphate and sodium humate during WS autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~ 75.4–77.2% to 47.3–57.7%.Conclusion The existence of different types soil conditioner model compounds results in various component fractions from autohydrolyzed WS in the process of autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS autohydrolysis and enzymatic hydrolysis.
研究领域:
生物化学
·
农业科学
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DOI
高影响因子
Journal of Cleaner Production
·
2025
·
IF 10
Shuyang Shi,Baozhong Ma,Hui Yang,Zhihe Cao,Yubo Liu,Yongqiang Chen,Chengyan Wang
In bauxite-scarce China, aluminum extraction from alternative resources via the acid leaching process is promising. However, critical gaps persist: (1) pyrolysis yields lightweight alumina with limited market capacity, and (2) direct electrolytic alumina synthesis from acid leach liquor remains unexplored, especially alumina particle size control. The production of coarse boehmite as an alumina precursor via high-temperature autohydrolysis was investigated in this paper. First, the hydrolysis characteristics of Al 3+ were revealed by thermodynamic analysis. Subsequently, the experimental parameters and hydrolysate particle size were optimized through a series of experiments. Under optimal conditions, an Al hydrolysis ratio of 82.6 % and a median particle size of 27.1 μm for polycrystalline boehmite were achieved. The morphological evolution of boehmite was revealed by SEM. The hydrolysis path was determined by analyzing the variation of Al-ion species with pH. Kinetics demonstrated that the hydrolysis process followed heterogeneous nucleation and was chemically controlled, with an apparent activation energy of 52.2 kJ/mol. Finally, a novel process for electrolytic Al 2 O 3 preparation and simultaneous HNO 3 regeneration using coal gangue and fly ash as raw materials was proposed. This study establishes a technological foundation for producing electrolytic alumina from aluminum-containing solid wastes, providing a novel route for large-scale aluminum resource recovery.
研究领域:
化学工程
·
材料科学
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产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2023
·Vol. 25
·期 15
·5968-5978
·
IF 9.8
Jiahui Wei,Haonan Zhang,Shengcheng Zhai,Hao Ren,Huamin Zhai
Pure subcritical water autohydrolysis is an economical and green biorefinery method and potentially applicable technology. Elucidating and regulating the energy input effects on the structural dissociation and chemical depolymerization of lignocellulosic biomass will progress the industrialization of pure subcritical water autohydrolysis biorefineries. In this work, a method for determining the degree of tissue dissociation was invented. Combined with the analysis of the microstructure, ultrastructure, chemical composition, and aggregation state, this paper reveals the process and mechanism of the P-factor effect as an energy input measurement on the bio-structural dissociation and chemical depolymerization during naked oat stem autohydrolysis. A method of using the P-factor to regulate the biostructural dissociation and chemical depolymerization was developed. For the first time, a critical point for the autohydrolysis of naked oat stem in pure subcritical water between 170–210 °C was found to be at a P-factor = 233, around which an essential change in biostructural dissociation and chemical depolymerization occurred. The findings indicate that the control of naked oat-stem tissue and cell dissociation, ultrastructure, and chemical depolymerization can be accomplished using the P-factor as an energy input measurement for autohydrolysis. The revealed mechanism and method created in this study enable the stepwise separation of gramineae tissues, cells, and major chemical components, enabling a full-composition multi-purpose biorefinery of lignocellulose.
研究领域:
生物质能
·
植物化学
·
绿色化学工程
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Yaqian Cai,Qianyu Yu,Hanying Zhao
Self-assembly of proteins into well-organized proteinosomes has attracted great interest due to the potential medical and biological applications of the structures. Herein, a new concept of electrostatic assisted fabrication of proteinosomes is proposed. The self-assembly is performed by using multi-step dialysis approach, where negatively charged bovine serum albumin-poly( N -isopropylacrylamide) (BSA-PNIPAM) bioconjugate and positively charged enzyme (lysozyme or trypsin) are initially dissolved in phosphate buffer (PB) solution at a high salt concentration, and subsequently the protein solution is dialyzed against PB solutions at low salt concentrations, resulting in the formation of biofunctional proteinosomes. Transmission electron microscopy (TEM), cryo-TEM and light scattering results all demonstrate the formation of hollow structures. The wall of a proteinosome is composed of BSA and enzyme (lysozyme or trypsin), and PNIPAM chains of the bioconjugate are in the corona stabilizing the structure. In comparison with the native enzymes, the enzyme molecules in the assemblies basically retain their bioactivities. The proteinosomes formed by BSA-PNIPAM and lysozyme can be dissociated in the presence of trypsin, and those self-assembled by BSA-PNIPAM and trypsin are able to be self-hydrolyzed, resulting in the dissociation of the structures in aqueous solution. The size and morphology changes of the proteinosomes in the hydrolysis are studied.
研究领域:
生物技术
·
材料科学
·
生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）