Journal of Analytical Science and Technology
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2024
·Vol. 15
·期 1
·1-8
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IF 2.5
Li Yunxiao,Wang Qiuxia,Wang Yali
The abnormally expressed microRNAs (miRNAs) serve as crucial indicators for disease diagnosis and are strongly associated with the progression of various diseases. The quantification of miRNAs is highly significant for the clinical diagnosis and treatment of various types of cancers. This study utilizes the CRISPR/Cas13a system, which combines CRISPR RNA (crRNA) and CRISPR-associated Cas13a, to recognize the miRNA directly and specifically, thus activating the trans-cleavage activity of Cas13a. By integrating the CRISPR-Cas13a system with an exonuclease-iii (Exo-iii)-assisted chain cleavage of silver ions (Ag+)-aptamer and an Ag+ -based color reaction, a method for the detection of miRNA that is specific and sensitive is developed. This approach demonstrated exceptional sensitivity in detecting miRNA, with a low detection limit of 5.12 fM. Moreover, the suggested method was effectively utilized to precisely measure the amount of miRNA in intricate biological samples, demonstrating its practical applicability. Furthermore, due to its elevated sensitivity and simple probe design and fluorophore labeling, the suggested colorimetric technique holds great potential for use in clinical diagnostics.
研究领域:
分子生物学
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医学诊断
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生物技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2016
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IF 4.712
Ru-Jia Yu,Wei Ma,Mao-Pan Peng,Zhi-Shan Bai,Yi-Tao Long
A novel redox-mediated chromogenic reaction was demonstrated based on the reaction between HAuCl 4 and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), which generate various color responses from red to green in the resulting solutions. Various redox substance could be used to mediate the reaction and trigger a distinct color response. We established a sensitive hydrogen peroxide colorimetric sensor based on the redox-mediated chromogenic reaction and depicted the application both in detection of enzyme and in an immunoassay . Combining the traditional chromogenic reagent with gold nanoparticles , our assay has the advantage in short response time (within three minutes), high sensitivity (10 −12 g mL −1 for HBsAg) and stability.
研究领域:
生物化学
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医学检测
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纳米技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
·
IF 4.098
Min Huang,Fenghua Geng,Yongxiang Wang,Congying Shao,Gen Liu,Maotian Xu
The photometric method is widely used in real clinical tests due to its simple operation, low cost and convenient. Many of the reported colorimetric ALP assays so far are non- ratiometric because the detection was based on changes in absorbance at a single wavelength. The development of novel colorimetric and ratiometric assay is of importance for quantitatively measuring target with high accuracy. The challenge in the design of ratiometric photometric assay is that the chromophore must have a significant spectral shift before and after binding to the target. Here, we report a colorimetric and ratiometric photometric sequential assay of AA and ALP based on the complexation between ARS and Cu 2+ and redox reaction between AA and Cu 2+ . The absorption band of ARS centered at 425 nm (yellow color), which could be shifted to 510 nm (red color) upon Cu 2+ binding. However, as far as we know, this classic color reaction has not been used to develop a ratiometric photometric method to sequentially detect AA and ALP, although photometric methods based on the regulation of other color reagents with oxidizing metal ions have been reported. The proposed sensing system shows a limit of detection for ALP at 0.24 U L −1 and could be applied for detecting ALP in newborn calf serum. The established sensing system makes a useful contribution to the detection of ALP in complex clinical samples.
研究领域:
临床检验
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生物化学
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医学诊断
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DOI
PubMed
高影响因子
SENSORS
·
2018
·Vol. 18
·期 8
·2679
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IF 2.475
Zheng-Jun Xie,Xian-Yu Bao,Chi-Fang Peng
A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+by centrifugation. It was found that the CH3Hg+reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+detection in environmental samples.Keywords:methylmercury;gold nanoparticles;enzyme mimic;chromogenic reaction
研究领域:
环境科学
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纳米生物学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2017
·
IF 2.625
Mi Zhang,Xiaobing He,Jie Wei
In this paper, we have developed a paper-based optical imaging material responding to visible light. This material consists of a thioxanthone (TX) derivative with the additional phenyl carbazole chromophore, namely 1-phenyl-11,12a-dihydrothiochromeno (3,2-b) carbazol-13 (5H)-one (TX-PC), and leuco crystal violet (LCV). TX-PC is newly synthesized and characterized by 1 HNMR, FTIR and high-resolution mass spectrometer. As a visible light absorbing Type II photoinitiator, TX-PC has an excellent absorption property in the visible region in the absence of an added co-initiator. In other words, TX-PC degrades into lively free radicals when irradiating under visible light which resulting in a color reaction on LCV . The degree of reaction is characterized by UV–vis spectrophotometer. In this study, this material is used for imaging within visible light because of the high efficiency color reaction. We coat a paper substrate with this TX-PC/LCV solution and cover with a pattern photomask. Under the irradiation of the visible light, color reaction occurs to irradiated area while the rest remains colorless. Thus the two areas are distinguished to obtain a clear image as same as the original version.
研究领域:
材料科学
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光学
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DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2020
·
IF 0.936
Jia-Hui ZHAO,Guo-Yong LIU,Shuang WANG,Sha-Sha LU,Jian SUN,Xiu-Rong YANG
Dopamine is a crucial monoamine neurotransmitter involved in multiple cognitive functions and pathological processes. Herein, we described a rapid chromogenic and fluorogenic reaction between dopamine and naphthoresorcinol under ambient conditions, and found that the product possessed intense visible absorbance and high fluorescence quantum yield (78%) in aqueous solution. Through electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis, the plausible reaction mechanism between dopamine and naphthoresorcinol was deduced, and the structure of the reaction product was confirmed. Inspired by the explicit mechanism and accessible reaction conditions, a straightforward and selective assay for dopamine concentration was developed with colorimetric and fluorescent dual-modal signals based on the in situ reaction.
研究领域:
生物化学
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分析化学
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神经科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2020
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IF 6.785
Yong Liu,Jiguang Li,Guangfa Wang,Baiyi Zu,Xincun Dou
Although colorimetric detection based on reagents has been widely used in the fields of practical trace analysis, its versatility for detecting multitargets remains the most challenging problem. As a proof of concept, a general colorimetric reagent based on potassium isopropanol (C3H7KO) and dimethyl sulfoxide for one-step instantaneous detection and discrimination of typical military and improvised explosives was designed. Vivid colors from none to purple red, blue green, yellow green, and green were shown, respectively, when detecting 2,4,6-trinitrotoluene (TNT), 2,4-dinitrotoluene (DNT), elemental sulfur (S), and potassium permanganate (KMnO4). The unique design including the specific nucleophilic addition reaction and the base-catalyzed oxidation-induced electron transfer ensures perfect selectivity even upon facing more than 20 interferents. It is further experimentally demonstrated that the confinement effect introduced by Tween-20 plays an essential role in enhancing the color signal on the surface and thus boosts the detection performance even with a mass as low as 1.45 ng. The applicability of this versatile colorimetric reagent was further verified by integrating the reagent onto paper strips for the in-field identification of TNT, DNT, S, and KMnO4 with the help of a portable smartphone-based microscope apparatus, and a practical detection mass of 10.3 ng could be realized. We expect the present colorimetric reagent design strategy would pave a way for one-step instantaneous visual detection toward trace multianalytes.
研究领域:
分析化学
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爆炸物检测技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Yuping Sun,Jianhui Wang,Qianying Lu,Jiaxue Zhang,Yanyan Li,Yushuang Pang,Cheng Yang,Qian Wang,Desheng Kong
Stretchable sweat sensors are promising technology that can acquire biomolecular insights for health and fitness monitoring by intimate integration with the body. However, current sensors often require microfabricated microfluidic channels to control sweat flow during lab-on-body analysis, which makes effective and affordable sweat sampling a significant practical challenge. Here, we present stretchable and sweat-wicking patches that utilize bioinspired smart wettable membranes for the on-demand manipulation of sweat flow. In a scalable process, the membrane is created by stacking hydrophobic elastomer nanofibers onto soft microfoams with predefined two-dimensional superhydrophobic and superhydrophilic patterns. The engineered heterogeneous wettability distribution allows these porous membranes to achieve enhanced extraction and selective collection of sweat in embedded assays. Despite the simplified architecture, the color reactions between sweat and chemical indicators are inhibited from directly contacting the skin to achieve a largely improved operation safety. The sensing patches can simultaneously quantify pH, urea, and calcium in sweat through digital colorimetric analysis with smartphone images. The construction with all compliant materials renders these patches soft and stretchy to achieve conformal attachment to the skin. Successfully analyzing sweat compositions after physical exercises illustrates the practical suitability of these skin-attachable sensors for health tracking and point-of-care diagnosis.
研究领域:
生物医学工程
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材料科学
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传感技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2017
·Vol. 64
·期 7
·795-803
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IF 0.935
Junyu Lu,Lianqiang Wei,Dongmei Yao,Xiuju Yin,Hongfang Lai,Xiuxiang Huang
β-AgVO 3 nanorods have been demonstrated to exhibit intrinsic peroxidase-like activity. The oxidation of glucose can be catalyzed by glucose oxidase (GO x ) to generate H 2 O 2 in the presence of O 2 . The β-AgVO 3 nanorods can catalytically oxidize peroxidase substrates including o -phenylenediamine (OPD), 3,3′,5,5′-tetramethylbenzidine (TMB), and diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) by H 2 O 2 to produce typical color reactions: OPD from colorless to orange, TMB from colorless to blue, and ABTS from colorless to green. The catalyzed reaction by the β-AgVO 3 nanorods was found to follow the characteristic Michaelis–Menten kinetics. Compared with horseradish peroxidase and AgVO 3 nanobelts, β-AgVO 3 nanorods showed a higher affinity for TMB with a lower Michaelis–Menten constant ( K m ) value (0.04118 mM) at the optimal condition. Taking advantage of their high catalytic activity, the as-synthesized β-AgVO 3 nanorods were utilized to develop a colorimetric sensor for the determination of glucose. The linear range for glucose was 1.25–60 μM with the lower detection limit of 0.5 μM. The simple and sensitive GO x -β–AgVO 3 nanorods–TMB sensing system shows great promise for applications in the pharmaceutical, clinical, and biosensor detection of glucose.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2013
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IF 2.894
Qi Han,Wenyi Dong,Hongjie Wang,Tongzhou Liu,Feiyun Sun,Yulei Ying,Xiangling Yan
In this study, the effects of anions on the decolorization reaction by potassium ferrate(VI) were investigated for the first time and a comparative investigation between potassium ferrate(VI) and potassium permanganate was carried out. Anions such as Cl − , SO 4 2 - , and NO 3 - showed favorable effects on color removal, while, CO 3 2 - and PO 4 3 - displayed inhibitory effects. The effects of the oxidant concentration, initial solution pH, dye concentration and temperature on color removal were studied, and the removal efficiencies of chemical oxygen demand (COD) and total organic carbon (TOC) were also examined. The decolorizing capacity of ferrate(VI) was better than potassium permanganate. When dye concentration was 100 mg/L, oxidants were both 150 mg/L, 100% color removal was achieved by K 2 FeO 4 treatment while only 62.04% by KMnO 4 . Ferrate(VI) has stably high decolorizing efficiency up to 85% in a wide pH range from 4.0 to 9.0. However, potassium permanganate only had better efficiency under acidic solution (100% color removal at pH 3.0, while only 78.05% at pH 9.0). At the temperature of 60 °C, approximately 80% decolorizing efficiency was achieved by ferrate(VI) while potassium permanganate only 59.37%. Ferrate(VI) acquired 58.33% COD and 24.15% TOC removal while potassium permanganate only 42.86% and 9.94% respectively. The oxidizing order was that color > COD > TOC both for K 2 FeO 4 and KMnO 4 .
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Dan Huo,Xipeng Zhang,Jiaxin Wei,Jinhua Wang,Qiang Zhang,Qiulin Yang,Hongxiang Zhu,Fengshan Zhang,Guigan Fang,Ting Wu
The toxic volatile organic compounds (VOCs), especially formaldehyde (FA), released from decoration materials pose a great threat to human health. In this study, formaldehyde adsorption performance of the specially formulated nanocellulose/chitosan aerogel (CNFCA) was investigated in simulated atmosphere. The physicochemical property of the composite aerogel was characterized, which had a large specific surface area (153.67 m 2 /g), a rough surface and an ultra-thin and porous structure. The composite aerogel showed excellent adsorption capacity for the formaldehyde, its theoretical maximum adsorption capacity was as high as 83.89 mg/g, and the adsorption process was more in accordance with the pseudo-second-order kinetics. The chromogenic reaction between the 4-amino-3-benzo-5-mercapto-1,2,4-triazolium (AHMT) and CNFCA was found that the color of the composite aerogel was depended on the free formaldehyde concentration. Based on this phenomenon, a colorimetric card was proposed and built to detection the formaldehyde in the atmosphere. Moreover, the adsorption mechanism research was found that the CNFCA with a multilayer structure belonged to physicochemical complex adsorption.
研究领域:
材料科学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2016
·Vol. 63
·期 1
·257-265
·
IF 2.98
Haizhou Lin,Qingang Xiong,Yuan Zhao,Jingping Chen,Shurong Wang
In this work, a green reaction system of CO 2 -water-isopropanol was developed for 5-hydroxymethylfurfural (HMF) production. The conversion of fructose in a CO 2 -water system was first investigated, and the results showed this system could promote the formation of HMF compared to a pure water system. Then, isopropanol was introduced into the CO 2 -water system and the HMF formation became better because the solvent effect of isopropanol increased the tautomeric composition of fructofuranose, which was easy to form HMF. The existence of isopropanol was found to greatly suppress secondary reactions where HMF was converted to levulinic acid and insoluble humin. Meanwhile, the effects of reaction parameters on the conversion of fructose to HMF in the CO 2 -water-isopropanol system were analyzed, and a high HMF yield of 67.14% was obtained. Finally, to further illustrate the merits of CO 2 -water-isopropanol system, productions of HMF from other carbohydrates were tested and satisfactory yields were achieved. © 2016 American Institute of Chemical Engineers AIChE J , 63: 257–265, 2017
研究领域:
化学工程
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可再生化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）