Chinese Journal of Natural Medicines
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2021
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IF 3
Di ZHANG,Bing YANG,Shi-Quan CHANG,Sheng-Suo MA,Jian-Xin SUN,Lin YI,Xing LI,Hui-Mei SHI,Bei JING,Ya-Chun ZHENG,Chun-Lan ZHANG,Feng-Guo CHEN,Guo-Ping ZHAO
This study was to investigate the protective effect of paeoniflorin (PF) on hydrogen peroxide-induced injury. Firstly, “SMILES” of PF was searched in Pubchem and further was used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets. Injury-related molecules were obtained from GeenCards database, and the predicted targets of PF for injury treatment were selected by Wayne diagram. For mechanism analysis, the protein-protein interactions were constructed by String, and the KEGG analysis was conducted in Webgestalt. Then, cell viability and cytotoxicity assay were established by CCK8 assay. Also, the experimental cells were allocated to control, model (200 μmol·L −1 H 2 O 2 ), SB203580 10 μmol·L −1 (200 μmol·L −1 H 2 O 2 + SB203580 10 μmol·L −1 ), PF 50 μmol·L −1 (200 μmol·L −1 H 2 O 2 + PF 50 μmol·L −1 ), and PF 100 μmol·L −1 (200 μmol·L −1 H 2 O 2 + PF 100 μmol·L −1 ) groups. We measured the intracellular ROS, Hoechst 33258 staining, cell apoptosis, the levels of Bcl-xl, Bcl-2, Caspase-3, Cleaved-caspase3, Cleaved-caspase7, TRPA1, TRPV1, and the phosphorylation expression of p38MAPK. There are 96 potential targets that may be associated with PF for injury treatment. Then, we chose the “Inflammatory mediator regulation of TRP channels” pathway for the experimental verification from the first 10 KEGG pathway. In experimental verification, H 2 O 2 decreased the cell viability moderately ( P < 0.05), and 100 μmol·L −1 PF increased the cell viability significantly ( P < 0.05). Depending on the difference of intracellular ROS fluorescence intensity, PF inhibited H 2 O 2 -induced reactive oxygen species production in Schwann cells. In Hoechst 33258 staining, PF reversed the condensed chromatin and apoptotic nuclei following H 2 O 2 treatment. Moreover, Flow cytometry results showed that PF could substantially inhibit H 2 O 2 induced apoptosis ( P < 0.05). Pretreatment with PF obviously reduced the levels of Caspase3, Cleaved-caspase3, Cleaved-caspase7, TRPA1, TRPV1, and the phosphorylation expression of p38MAPK after H 2 O 2 treatment ( P < 0.05), increased the levels of Bcl-2, and Bcl-xl ( P < 0.05). PF inhibited Schwann cell injury and apoptosis induced by hydrogen peroxide, which mechanism was linked to the inhibition of phosphorylation of p38MAPK.
研究领域:
神经生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
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2022
·
IF 9.336
Yu Zhao,Haofang Wan,Jiehong Yang,Yan Huang,Yu He,Haitong Wan,Chang Li
Natural deep eutectic solvent (NaDES) is widely applied in the extraction of nutrients from natural resources as a greener alternative for fossil solvent. In the present work, 27 different NaDESs were screened for the extraction of paeoniflorin (PF) and galloyl paeoniflorin (GPF) from Radix Paeoniae Rubra (RPR). After screening and extraction parameter optimization, the extraction yields of PF and GPF reached up to 182.8 mg/g and 77.4 mg/g with the selected NaDES, ChCl-Sor. Furthermore, the antioxidant activity in vitro and neuroprotectivity in vivo of the ‘ready-to-use’ extracts were evaluated comprehensively. Especially in vivo , the cerebral ischemic/ reperfusion injury model was established in rats and the protective effects of the RPR extracts were determined. The results not only proved that NaDES is a valuable green extraction media, but also indicated the safety and potential pharmaceutical application of NaDES based ‘ready-to-use’ extracts from medical plants.
研究领域:
药理学
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神经科学
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绿色化学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2017
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IF 3.359
Fengli Chen,Kailin Mo,Qiang Zhang,Shimin Fei,Yuangang Zu,Lei Yang
In the present work, a simple and efficient method for steam distillation of paeonol and simultaneous extraction of paeoniflorin from the root bark of Paeonia suffruticosa Andr. (Cortex Moutan) was developed using an ionic liquid as the reaction medium and microwave irradiation. A series of 1-alkyl-3-methylimidazolium ionic liquids with different anions and alkyl chain lengths were evaluated for this method. The optimum conditions were 1.25 M 1-butyl-3-methylimidazolium bromine as the extraction solvent, 20 mL/g liquid–solid ratio, 25 min microwave irradiation time, 700 W microwave irradiation power, and 60–80 mesh particle size. Under these conditions, satisfactory yields of paeoniflorin (80.49 ± 2.58 mg/g) and paeonol (10.52 ± 0.36 mg/g) were obtained. The initial purity of paeonol was 84.95%, and this increased to >99% with simple recrystallization. The ionic liquid extraction solution, either with or without trace paeonol, was passed through HPD100B macroporous resin for enrichment and separation of paeoniflorin and recycling of the ionic liquid used. Recycling and reuse of the ionic liquid were achieved successfully, which could reduce ionic liquid requirements and the process cost. The method was validated using stability, recovery, and repeatability experiments. The proposed method is very promising for separation of natural compounds, especially for small molecules that can be isolated by steam distillation.
研究领域:
植物化学
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天然产物化学
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中药学
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DOI
高影响因子
MOLECULES
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2016
·Vol. 22
·期 1
·5
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IF 2.465
Huifang Zhang,Xiaofang Li,Ke Wu,Mengke Wang,Pu Liu,Xinsheng Wang,Ruixue Deng
Paeonia ostiiis a traditional medicinal plant popularly used in China. This study intended to evaluate the antioxidant properties and the chemical components of the flavonoid-rich extracts from the flowers ofP. ostii. The results showed that the flavonoid-rich extracts from the flowers ofP. ostiihad strong scavenging capacities on 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), hydroxyls, superoxide anions, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner. Five flavonoids, dihydrokaempferol (1), apigenin-7-O-β-d-glucoside (2), apigenin-7-O-β-d-neohesperidoside (3), kaempferol-7-O-β-d-glucopyranoside (4), and kaempferol-3-O-β-d-glucopyranosyl-7-O-β-d-glucopyranoside (5), were isolated from the flavonoid-rich extracts of the flowers ofP. ostii. High-performance liquid chromatography (HPLC) analysis revealed that compounds3and4were abundant in theP. ostiiflower and in flavonoid-rich extracts. The main components of the flower ofP. ostiiare flavonoids. The high antioxidant activity of the flavonoid-rich extracts may be attributed to the high content of flavonoids. The five isolated flavonoids were the primary antioxidant ingredients, and may play important roles in the strong antioxidant activities of this flower. Based on the obtained results, the flower ofP. ostiicould be a potential source of natural antioxidants in food and pharmaceutical applications.Keywords:flavonoids of peony flower;Paeonia ostii;natural antioxidant;chemical constituents
研究领域:
植物化学
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药理学
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DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Wei Zhu,Sai Zhu,Shan Jiang,Jiuyu Yin,Yonggui Wu,Xiaomei Luo
Background Cisplatin (CIS), a widely administered chemotherapeutic agent, is associated with acute kidney injury (AKI). Lansoprazole (LPZ), commonly prescribed to mitigate chemotherapy-induced gastrointestinal complications, may further elevate AKI risk. Paeoniflorin (Pae), a bioactive compound derived from traditional Chinese medicine, demonstrates renal protective properties in AKI, yet its mechanism against CIS and LPZ induced AKI remains undefined. Methods A composite AKI model was established in vivo and in vitro using CIS and LPZ. The protective effect of Pae on the injury of renal tissue or renal tubular cells in mice was observed, followed by Pae pretreatment. The target of Pae was found through network pharmacology, and the binding ability of the target was verified by CETSA experiment and molecular docking. Finally, the target gene was knocked out or over expressed to verified whether Pae regulates necroptosis through this target. Results It was found that renal injury could be exacerbated by LPZ in the CIS induced AKI. Pae could alleviate CIS and LPZ induced AKI. The target gene of Pae was TNFAIP3, which played an important role in necroptosis. The TNFAIP3 could bind well to Pae in molecular docking and CETSA experiment. The necroptosis and inflammatory responses in AKI induced by CIS and LPZ could be also inhibited by Pae in vivo and in vitro. Overexpression of TNFAIP3 played the same cellular protective role as Pae preconditioning. In contrast, Pae could not continue to play a protective role after TNFAIP3 was knocked down. Conclusion The traditional Chinese medicine Pae holds promise as a potential therapeutic agent for AKI, with TNFAIP3 representing an effective therapeutic target in this pathology.
研究领域:
药理学
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分子生物学
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肾脏病学
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转化医学
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肿瘤学
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DOI
PubMed
高影响因子
BIOMATERIALS
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2020
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IF 10.317
Sha Xiong,Zhongjun Li,Yao Liu,Qun Wang,Jingshan Luo,Xiaojia Chen,Zhongjian Xie,Yuan Zhang,Han Zhang,Tongkai Chen
The treatment of Parkinson's disease (PD) is hampered by the blood-brain barrier (BBB). As such, there is an urgent need for the development of a novel nanoplatform capable of penetrating the BBB in order to effectively treat PD. In the present study, we utilized black phosphorus nanosheets (BP) containing the brain-targeting ligand lactoferrin (Lf) and loaded with Paeoniflorin (Pae) to obtain Lf-BP-Pae. Through an effective photo-thermal effect, these Lf-BP-Pae particles were capable of traversing the BBB and effectively treating PD in a targeted manner. Importantly, this BP-based nanoplatform was capable of achieving satisfactory biocompatibility and biosafety with excellent anti-Parkinsonian efficacy, making it ideal for clinical applications.
研究领域:
神经科学
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纳米医学
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药物递送
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DOI
PubMed
高影响因子
Journal of Essential Oil Bearing Plants
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2019
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IF 0.688
Gaoming Lei,Chaoying Song,Haiyu Shi,Yihao Xing,Xue Song,Guojin Liang
Parameter effects and kinetics of ultrasound assisted ionic liquid mediated hydro-distillation (UILHD) were investigated. An ionic liquid 1-ethyl-3-methylimidazolium bromide ([C2C1im]Br) was employed both as a substrate under sonication and as an additive during distillation. Paeonia suffruticosa Andr. ‘Jitsugetsu Nishiki’, a widely cultivated ornamental plant in China, was selected as a case study. The optimal condition of UILHD was [C2C1im]Br concentration of 10 %, ultrasonic power of 400 W and pretreatment duration of 20 min. Under such a condition, the extraction equilibrium could be basically reached at 35 min of distillation. The kinetics obeyed pseudo-first-order equation. Calculated values of maximum essential oil yield and first-order rate constant were 1.53 g and 0.117 min-1, respectively. The main essential oil components of flowers of P. suffruticosa Andr. ‘Jitsugetsu Nishiki’ were 1,3,5-trimethoxybenzene (16.6 %), tricosane (8.7 %), nerol (8.6 %), nonadecane (6.4 %) and nonanal (5.5 %). The most abundant chemical class was non-terpene hydrocarbons (44.6 %) followed by non-terpene oxygenated compounds (31.2 %) and oxygenated terpenes (22.2 %). UILHD proved to be a promising technique for efficient isolation of essential oils.
研究领域:
植物化学
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提取技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2017
·Vol. 52
·期 11
·2352-2361
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IF 1.64
Xiaoli Sun,Wengang Li,Jian Li,Yuangang Zu,Xiuhua Zhao
Summary In this study, β-cyclodextrin (β-CD) was used to form an inclusion complex with peony seed oil (PSO), in order to improve its stability, transport convenience and bioavailability. Single-factor optimisation method was applied to determine the optimum inclusion conditions. The inclusion complexes were prepared by saturated solution method and characterised by GC-MS, SEM, FT-IR, XRD, DSC and 1 H NMR analyses. All results suggested that PSO was successfully entered into the β-CD cavity. What is more, inclusion rate of PSO reached 68.72 ± 2.14%, resulting in a product of 25.97% PSO content, and the unsaturated fatty acids, similar to PSO, accounted for 90.05% of all fat acids in complex. The linolenic acid in inclusion complex exhibited 64.84% higher bioavailability than that in PSO. The inclusion complex possessed of better stability for storage and transportation. Overall, the fabricated complex improved the feature of PSO and thus could be used for novel applications.
研究领域:
食品科学
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药物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Xiaofeng Min,Li Lu,Xuetao Xu,Yi Wen,Xi Zheng
Paeonol , as one effective tyrosinase inhibitor, had been used as food preservative and clinical medication for skin disorders. In this study, the inhibition mechanism and binding behavior of paeonol to tyrosinase and its anti-browning property were investigated using multi-spectroscopic and molecular docking methods. Activity assay and kinetic results confirmed paeonol as a reversible mixed-type tyrosinase inhibitor. Results of the mechanistic studies were clarified using fluorescence quenching , synchronous fluorescence, CD spectra and 3D fluorescence, and showed that the binding of paeonol to tyrosinase might change the chromophore microenvironment and conformation of tyrosinase to inhibit enzyme catalytic activity. Molecular docking results revealed the detailed binding between paeonol and tyrosinase. Moreover, paeonol could prevent the browning of fresh-cut apples, as well as inhibiting PPO and POD activities and increasing APX activity. All above findings established a reliable basis for the inhibitory mechanism of paeonol against tyrosinase and therefore contributed to its application in anti-browning.
研究领域:
食品科学
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生物化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2025
·Vol. 26
·期 1
·1-17
·
IF 4
Gu Qifei,Xiao Wuqing,Zhu Yingyin,Zhong Wanling,Sui Xue,Liu Yongchao,Zhang Jie,Wu Huichao,Zhou Rui
Paeoniflorin is a natural pharmaceutical ingredient with a widely biological activity. However, as a hydrophilic drug, the problem of low transdermal rate limits its clinical application. To overcome this shortage, LUVs were used as biocompatible carriers of paeoniflorin in this study. We prepared paeoniflorin-loaded large unilamellar vesicles (LUVs) with W/O/W structure by microfluidics. We used four kinds of chips to prepare paeoniflorin LUVs and explored the effects of the chip structures on LUVs properties applying both experiments and numerical simulations. The difference of fluid mixing mechanisms was analyzed among four different channels, including straight and curved structures. Then we evaluated the differences in skin permeability among the three groups, paeoniflorin aqueous solution group, drug-loaded liposome group and blank liposome & drug mixture group, using the abdominal skin of male mice. The results showed that the structure of the microfluidic channel was a key factor affecting the flow rate and mixing efficiency. The mixing efficiency further affected the liposome size. The mixing efficiency of curved channel was not better than that of a straight channel due to the low flow rate and long mixing time. By the results of transdermal experiments, LUVs could reduce the transdermal time and increase the total transdermal amount. LUVs effectively improved the transdermal absorption efficiency of paeoniflorin. In conclusion, paeoniflorin LUVs with highly efficient transdermal were successfully prepared by using microfluidics. We explored the underlying fluid dynamics that lead to variations in the preparation with different chip structures. The transdermal effect of the LUVs was verified. Graphical
研究领域:
药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
INFLAMMOPHARMACOLOGY
·
2018
·Vol. 27
·期 5
·969-974
·
IF 3.304
Zhao Mingyi,Zhou Peng,Yu Jun,James Asenso,Xiao Feng,Wang Chun,Wei Wei
Paeoniflorin-6′-O-benzene sulfonate (code: CP-25) is a novel ester derivative of paeoniflorin (Pae). Compared to Pae, CP-25 has higher lipid solubility, bioavailability and better bioactivity. However, the tissue distribution and excretion of CP-25 still remain unknown. The LC–MS method was applied to investigate the tissue distribution and excretion of CP-25 in rats. As such, 50 mg/kg of CP-25 and Pae were administered to rats in multiple doses via an oral route. CP-25 and Pae were distributed widely and rapidly in all the tested tissues. Compared with Pae, the concentrations of CP-25 were almost increased evidently in most tissues. The highest CP-25 level was found in the liver (1476.33 ± 535.20 ng/g, male; 1970.38 ± 177.21 ng/g, female) at 3 h, and a high concentration of CP-25 was detected in male and female intestine, synovium, muscle, lung, and brain. Following a single oral dose of 50 mg/kg of CP-25 in rats, the total excretion of CP-25 was merely 21.8% (18.40, 3.19 and 0.22% for feces, bile and urine, respectively) in males; and was approximately 21.3% (14.04, 7.16 and 0.14% for feces, bile and urine, respectively) in females. The results indicated that the CP-25 concentration was higher in major tissues than Pae; CP-25 was primarily excreted through the feces; and there were gender-related differences in the tissue distribution and excretion.
研究领域:
药理学
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中药学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2016
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IF 3.138
Jun-Jun Shi,Jian-Guo Zhang,Yu-Han Sun,Jie Qu,Ling Li,Chandan Prasad,Zhao-Jun Wei
The sequential extraction of peony seed dreg polysaccharides (PSDP) with hot buffer (HBSS), chelating agent (CHSS), dilute alkaline (DASS) and concentrated alkaline (CASS) yielded four different polysaccharide fractions. Based on their absorptions at 3600–3200 cm −1 and 1200–800 cm −1 , these fractions were confirmed to be polysaccharides . The properties of four PSDPs displayed some slight differences. The CASS showed the highest peak temperature and endothermic enthalpy. The emulsifying activity and emulsifying stability of four PSDPs exhibited a dose-dependent pattern; HBSS showed the highest emulsifying activity, and CHSS displayed the longest emulsifying stability. The four PSDPs also exhibited wide variations in their antioxidant activities. For example, i) CASS showed the highest DPPH radical scavenging activity, reducing power and ABTS radical scavenging activity; ii) HBSS exhibited the highest hydroxyl radical scavenging activity, and iii) CHSS displayed the higher ferrous ions chelating ability than others.
研究领域:
食品科学
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天然产物化学
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碳水化合物化学
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功能食品
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抗氧化研究
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）