JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2022
·Vol. 103
·期 2
·770-778
·
IF 4.125
Yuteng Fu,David Julian McClements,Shunjing Luo,Jiangping Ye,Chengmei Liu
BACKGROUND Rutin is a natural bioactive flavonoid that is poor in water solubility and chemical stability. Encapsulation can be used to protect bioactive molecules from chemical or physical decomposition during food processing and storage. Thus, the effect of initial particle size on the ability of oil-in-water emulsions to retain rutin during storage was investigated. RESULTS Rutin was encapsulated in oil-in-water emulsions with different mean surface-weighted diameters: d 3,2 = 0.56 μm (small), 0.73 μm (medium), and 2.32 μm (large). As expected, the resistance of the emulsions to coalescence and creaming during storage increased as the particle size decreased due to weakening of the colloidal and gravitational forces acting on the droplets. The concentration of rutin in the emulsions decreased during storage (28 days), which was mainly attributed to photodegradation of the flavonoid. The loss of rutin from the emulsions during storage was fitted using a second-order equation. The rutin degradation rate constant k decreased and the half-life t 1/2 increased with decreasing droplet size, which was attributed to the stronger encapsulation and light scattering by smaller oil droplets reducing the amount of light that can penetrate into the emulsions. CONCLUSION This study has important implications for the design of more efficacious emulsion-based delivery systems for incorporating health-promoting nutraceuticals into foods. © 2022 Society of Chemical Industry.
研究领域:
食品科学
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化学工程
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营养学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Alkassoumi Hassane Hamadou,Jiyao Zhang,Chen Chao,Bin Xu
This work evaluated the effect of pectin (P) and chitosan (CH) dual coating on rutin (R) loaded nanoliposomes (NLs). Besides R loaded NLs (RNLs), CH coated RNLs (CH-RNLs) and P coated CH-RNLs (P–CH-RNLs) were prepared. The results showed a size increase of NLs due to the polymer coatings, confirming a successful deposition of CH and P onto the surfaces of NLs. A surface charge reversal was observed after coating RNLs and CH-RNLs with CH and P, respectively. The coating resulted in a significant decrease in encapsulation efficiency (EE) from 88.19% in RNLs to 78.89% in CH-RNLs and 73.44% in P–CH-RNLs. Due to protection provided by the P–CH barrier, P–CH-RNLs displayed better thermal, pH, ionic and oxidative stability. NLs were more stable at 4 °C as compared to 25 °C over 8 weeks, and P–CH-RNLs exhibited the best performance overall. This study revealed P–CH coating as a potential route to produce stable NLs for the delivery of hydrophobic rutin.
研究领域:
药剂学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
Frontiers in Pharmacology
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2025
·
IF 4.8
Yuxin Shen,Fengsheng Hong,Hualu Wu,Xiaohai Chen,Hailun Xia,Ren-ai Xu,Guanyang Lin,Lu Shi
This study aimed to examine the potential drug-drug interaction (DDI) between vandetanib and luteolin in vivo and in vitro, with the objective of establishing a scientific foundation for their appropriate utilization in clinical settings. Sprague-Dawley (SD) rats were randomly divided into two groups: a control group (vandetanib administered by gavage alone) and an experimental group (vandetanib and luteolin administered together). A series of blood samples were collected at different time intervals. The plasma concentrations of vandetanib and its metabolite N-demethyl vandetanib in rats were determined using an ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Incubation systems were set up with rat liver microsomes (RLM) and human liver microsomes (HLM) to measure the Michaelis-Menten constant (Km) and half-maximum inhibitory concentration (IC50) values. Additionally, the inhibitory mechanism of luteolin on vandetanib was also investigated. Ultimately, the molecular mechanism of inhibition was examined through the utilization of molecular docking techniques. In vivo animal experiment results showed that compared with the control group, the AUC(0-t) and Cmax of vandetanib in the experimental group were significantly increased. The findings from the in vitro experiments revealed that luteolin exhibited a moderate inhibitory effect on the metabolism of vandetanib. The IC50 values for RLM and HLM were determined to be 8.56 μM and 15.84 μM, respectively. The identified inhibition mechanism was classified as mixed. This study utilized molecular docking analysis to provide additional evidence supporting the competitive inhibition of luteolin on vandetanib in CYP3A4. The data presented in our study indicated a potential interaction between vandetanib and luteolin, which may necessitate the need for dose adjustment during their co-administration in clinical settings.
研究领域:
药理学
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临床医学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2020
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IF 3.306
Zhifeng Cai,Siying Chen,Xinru Ma,Dang Na,Jie Zhao,Tianqi Wu,Caifeng Zhang
For the first time, we developed a facile, green and low-cost strategy to prepare blue-green emitting fluorescence copper nanoclusters (Cu NCs) by using tyrosine as a protecting agent and hydrazine hydrate as a reducing agent. The tyrosine-protected Cu NCs (Tyr-Cu NCs) were analyzed through fluorescence spectroscopy, UV–vis absorption spectroscopy, fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The as-prepared Cu NCs was well dispersed and possessed good water solubility, excellent photostability and resistance to sodium chloride. The fluorescence intensity of Tyr-Cu NCs (around 480 nm under the excitation wavelength of 435 nm) was obviously quenched in the presence of rutin. According to this characteristic, a selective and sensitive fluorescence analytical method for the determination of rutin was established. The analytical method had a good linear relationship between F 0 /F and the concentrations of rutin (range from 0.1–60 μM) and the detection limit (LOD) was 0.016 μM. The possible mechanism of fluorescence quenching was caused by the IFE and static quenching. Meaningfully, the fluorescent probe was successfully employed to detect rutin in bovine serum samples.
研究领域:
分析化学
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生物医学
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产品关联
DOI
高影响因子
Journal of Environmental Sciences
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2022
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IF 6.796
Yunhao Zhang,Yi Qian,Ming Zhang,Weichuan Qiao
The wastewater discharge from the process of chrome plating, which contains 6:2 chlorinated polyfluorinated ether sulfonate (F-53B) and chromium (Cr), may be toxic to biofilm. In this study we found that the biofilm formed by Pseudomonas aeruginosa PAO1 was inhibited by exposure to a combination of F-53B and Cr(VI). The combined pollution damaged the cell membranes and the structure of the biofilm, and inhibited the production of the Pseudomonas quinolone-based signal, which affected biofilm formation. Moreover, the secretion of extracellular polymeric substances decreased as a result of this combined exposure. Exposure to F-53B and Cr(VI) individually or in combination could induce the excessive accumulation of intracellular reactive oxygen species (ROS), and the ROS positive rate of the bacteria increased under the treatment with 0.2 mmol/L of Cr(VI) and 250 nmol/L of F-53B, respectively. In addition, the activities of superoxide dismutase (SOD) and catalase (CAT) were enhanced for scavenging ROS in the bacteria that were exposed to Cr(VI) and F-53B. As an antioxidant, rutin was used to repair the toxicity of Cr(VI) and F-53B towards the biofilm formed by the bacteria. When rutin was added to the bacteria medium, with either Cr(VI) or F-53B as pollutant, or with the combined pollutants, the extracellular protein content of the bacteria recovered to 0.84, 0.94, and 0.85 times that of the control, respectively. Meanwhile, the accumulation of ROS and the activities of SOD and CAT decreased, which indicated that the addition of rutin can alleviate the oxidative stress and promote the antioxidant stress system.
研究领域:
环境科学
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微生物学
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毒理学
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DOI
PubMed
高影响因子
Italian Journal of Animal Science
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2022
·
IF 2.552
Shun Chen,Huijuan Liu,Jiaqi Zhang,Binbin Zhou,Su Zhuang,Xiaofang He,Tian Wang,Chao Wang
This study investigated the effects of different doses of rutin on the growth performance, immunity, intestinal barrier, and antioxidant capacity of broilers. A total of two hundred and fifty-six 1-day-old male broilers were divided into 4 groups and fed basal diets supplemented with 0 (control group), 250, 500 and 1,000 mg rutin/kg, respectively, for 42 days. In the starter period (days 1–21) and whole trial period (days 1–42), broilers fed diets containing 500 mg rutin/kg had significantly (p < 0.05) higher average daily feed intake, body weight and average daily gain and better feed conversion ratio. Dietary 500 mg rutin/kg increased the villus height, villus height to crypt depth ratio and villus area, while reducing the B cell lymphoma 2 associated X mRNA expression (p < 0.05). Dietary 500 mg rutin/kg increased the level of IgA in serum and decreased serum tumour necrosis factor-α (TNF-α) content, and the nuclear factor kappa-B, interleukin-2 and TNF-α mRNA expression in jejunal mucosa (p < 0.05). Simultaneously, 500 and 1,000 mg rutin/kg significantly decreased serum diamine oxidase activity, D-lactic acid and lipopolysaccharide concentration, increased activities of total superoxide dismutase and total antioxidant capacity in jejunal mucosa (p < 0.05), and upregulated mRNA expressions of genes related to intestinal barrier and antioxidant capacity in jejunal mucosa (p < 0.05). These findings indicate that dietary rutin, especially at 500 mg/kg, improves broilers’ growth performance, intestinal barrier function, immunity, and antioxidant capability, which may be related to the nuclear factor erythroid-2-related factor 2 (Nrf2) pathway.HighlightsDietary rutin improved growth performance, jejunal morphology, and intestinal barrier function.Dietary rutin enhanced the immunity via inhibiting NF-κB, and improved antioxidant capacity via activating Nrf2/HO-1 pathway in broilers.The optimal dose of rutin was 500 mg/kg in broiler diet.
研究领域:
动物营养学
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免疫学
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肠道生理学
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产品关联
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 5
·1055
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IF 4.6
Qi Chen,Hui He,Yanghong Zhu,Xiang Li,Junhao Fang,Zhexi Li,Panghui Liu,Lin Zhou,Yufang Pan,Guoyu Wu
Individuals infected with COVID-19 often experience the distressing discomfort of pharyngitis. Thus, it is crucial to develop novel drugs to improve therapeutic options. In this study, we investigated the interaction between bioactive compounds isolated from Acanthopanax trifoliatus (L.) Merr and proteins associated with COVID-19 and pharyngitis through in silico analysis. Several molecules demonstrated high affinities to multiple targets, indicating significant potential for alleviating pharyngitis and other COVID-19-related symptoms. Among them, rutin and isochlorogenic acid C, two major components in Acanthopanax trifoliatus (L.) Merr ethanol extracts, were further experimentally demonstrated to exhibit strong inhibitory effects against SARS-CoV-2 and to possess significant anti-inflammatory activities. Inhibition of over 50% in several key genes was observed, demonstrating the efficacy of in silico methods in identifying high-affinity target binders. Our findings provide a theoretical foundation for the development of Acanthopanax trifoliatus (L.) Merr as a novel multi-target therapeutic agent for both COVID-19 and pharyngitis.
研究领域:
药理学
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生物信息学
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病毒学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Lili Liu,Le Yang,Hui Chen,Weiwei Cheng,Yue Ding,Feng Xiao
Lysozyme (LYZ) is an excellent natural food preservative and can also be used as a bioactive carrier loading small molecules to enhance its stability and antioxidant properties. This research explored the intricate mechanism of interaction between LYZ and rutin. Multiple spectroscopic techniques was used first to confirm that rutin caused a fluorescence burst in LYZ. LYZ amino acid microenvironment was altered. The main driving forces driving the formation of the complex between rutin and LYZ were hydrogen bonding and van der Waals forces. In addition, the incorporation of rutin improved the overall stability and oxidation resistance of the complexes. The results of molecular docking and molecular dynamics simulation further show that rutin and LYZ are stably bound by hydrogen bonds and other interactions. The investigation contributed precious information for the development of novel natural preservatives and the design of advanced small molecular carriers.
研究领域:
食品科学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 36
·4736-4743
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IF 3.1
Wanyu Pang,Yali Gao,Tuoping Hu,Xuemei Ma
Rapid and accurate determination of luteolin is of great significance for pharmaceutical quality control. Herein, a disposable and sensitive luteolin sensor was fabricated by a hydrothermal method with carbon paper as substrate where ZIF-8 grew on GR in situ. Notably, the large specific surface area of ZIF-8 provided active sites on the electrode surface and the ability of GR to promote electron transfer greatly improved the sensitivity towards the oxidation of luteolin. Under the optimum conditions, the ZIF-8@GR/CP showed excellent detection performance for luteolin with a linear detection range of 0.04–3.2 μM and 3.2–120 μM, with LOD of 12 nM (S/N = 3). Furthermore, this disposable and sensitive sensor was successfully applied for the quantitative detection of luteolin in a capsule of Lamiophlomis rotata.
研究领域:
分析化学
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药学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Huijia Song,Haipin Zhang,Yulian He,Ruixia Gao,Yue Wang,Wenting Wang,Lisa D. Pfefferle,Xiaoshuang Tang,Yuhai Tang
Rutin (RT), a widely distributed natural flavonoid compound, has been generally utilized as an important active ingredient owing to its considerable biomedical and economic value. Inspired by the structure features of densely-packed bayberry and well-orientated honeycomb, a novel type of magnetic molecularly imprinted polymers (HB-TI-MMIPs) with abundant high-affinity and uniformly-distributed binding sites was rationally constructed for the selective enrichment of RT from Sophora japonica. The polymerization conditions, physicochemical properties, and adsorption performance of the imprinted nanomaterials were systematically investigated. The optimized HB-TI-MMIPs display a high adsorption capacity, fast adsorption rate, and satisfactory selectivity towards RT. Meanwhile, the proposed analytical methodology using HPLC, with HB-TI-MMIPs as adsorbents, successfully applied to enrich and detect RT from Sophora japonica with high recoveries (87.2–94.6%) and good RSDs (lower than 4.3%). Therefore, the fabricated HB-TI-MMIPs with a fast magnetic responsivity and desirable adsorption performance would be attractive in plant active ingredients extraction fields.
研究领域:
植物化学
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分析化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xinyu Lu,Yingying Li,Xuemin Duan,Yifu Zhu,Ting Xue,Liangmei Rao,Yangping Wen,Qingyun Tian,Yue Cai,Quan Xu,Jingkun Xu
A novel nanozyme sensor based on the electro-synthesized molecularly imprinted conducting poly(3,4-ethylenedioxythiophene) (PEDOT) nanocomposite with graphene-like two-dimensional layered molybdenum disulfide (MoS 2 ) was successfully prepared for electrochemical detection and analysis of luteolin in Gnaphalium affine. MoS 2 -MIPs were prepared facilely by electrochemical polymerization of monomer 3,4-ethylenedioxythiophene in the presence of both modifier MoS 2 and template molecule luteolin that can change the morphological structure and specific surface area of PEDOT, enhance electronic conductivity of MoS 2 , and improve the recognition capability and electrocatalytic capacity of electrode interface. The morphological structure of MoS 2 -MIPs was characterized by SEM, and its electrochemical performance and sensing parameters were investigated by voltammetry. The electrochemical response of luteolin was recorded by MoS 2 -MIPs in the linear concentration range from 3 × 10 -7 to 3 × 10 -5 Μ with limit of detection of 0.04 μΜ under the optimal conditions, which was successfully employed for electrochemically detecting luteolin in Gnaphalium affine with acceptable recoveries in comparison with high-performance liquid chromatography. This will provide a new nanozyme sensing platform comprised of electro-synthesized molecularly imprinted conducting polymers and graphene-like two-dimensional layered nanomaterials.
研究领域:
分析化学
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材料科学
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电化学
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产品关联
DOI
高影响因子
Current Research in Food Science
·
2023
·
IF 6.3
Yuchen Shangguan,Jing Ni,Lili Jiang,Yang Hu,Chuanbo He,Ying Ma,Guohong Wu,Hejian Xiong
In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed according to the Box-Behnken design of response surface methodology. The experimental conditions for flavonoid extraction were optimized, and naringin was separated from the extracted flavonoids using Sephadex LH-20 column chromatography. Experimental results showed that the influence of factors on the extraction rate of flavonoids from pomelo peels was in the order of ethanol concentration > solid-to-liquid ratio > extraction time, and the optimal extraction parameters were 85% ethanol concentration, 1:20 solid-to-liquid ratio, and 4-h extraction time for extracting flavonoids from pomelo peels. Under these conditions, the yield of flavonoids was 6.07 ± 0.06 mg/g. After three times of extraction, the flavonoid extraction rate reached 96.55%, and the residual naringin in the pomelo peels was 0.017 mg/g, at which point the bitterness in the pomelo peels disappeared. Two components, namely, PF1 and PF2, were separated from the crude flavonoid of pomelo peels through Sephadex LH20 column chromatography. PF2 was identified as naringin by high-performance liquid chromatography tandem mass spectrometry, with a purity of 95.7 ± 0.23%. Both flavonoids and PF2 exhibited good in vitro radicals scavenging activities on DPPH, ABTS, superoxide anion and hydroxyl.
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）