AMB Express
·
2018
·Vol. 8
·期 1
·1-8
·
IF 1.719
Liang Xiao-Sheng,Liu Chun,Long Zhu,Guo Xiao-Hua
Spore counting in probiotic Bacillus cultures using dipicolinic acid (DPA) as a marker was studied for developing a rapid and simple detection method. The newly developed method is based on the fluorescence enhancement by a new chelating agent, which forms a complex with EuCl 3 and DPA. The results showed that 1,2-cyclohexanediamine-N,N,N′N′-tetraacetic acid (CyDTA) greatly enhanced the fluorescence intensity in all selected chelating agents. The optimal composition of the fluorescence complex DPA-Eu-CyDTA had a detection limit of 0.3 nM of DPA. Metal ions in high concentrations, including Cu 2+ , Fe 2+ , Fe 3+ , Al 3+ , and Zn 2+ , might lower the detection sensitivity, which could be eliminated by diluting the sample with the metal ions below 10 μM. The maximum release of DPA was achieved by heating treatments at 121 °C for at least 10 min for two types of Bacillus endospores. The spore concentrations and corresponding released DPA fluorescence intensities were linearly associated (coefficient R 2 = 0.9993 and 0.9995 for Bacillus subtilis MA139 and Bacillus licheniformis BL20386, respectively). The detection limit for both strains reached approximately 6800 spores/mL. The verification results showed that the DPA fluorimetry assay developed in the present study was fully consistent with the plate-counting assay. The study shows that the fluorescence complex DPA-Eu-CyDTA can be reliably used for the detection of endospores in Bacillus fermentation for the production of probiotics.
研究领域:
微生物学
·
生物技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2025
·
IF 7.1
Haixu Zhang,Sen Yang,Xilu Zhang,Yaxin Pei,Mengxiao Chen,Hongge Chen,Jiran Zhang
Cyromazine, a widely used insect growth regulator, accumulates in livestock manure and poses risks to beneficial insects such as black soldier fly larvae (BSFL) during waste bioconversion. This study isolated two bacterial strains capable of degrading cyromazine, Bacillus subtilis CYR1 (CYR1) and Bacillus velezensis CYR2 (CYR2), from contaminated chicken manure. Both strains demonstrated significant degradation efficiency via hydrolysis of cyromazine to cyanuric acid, a less toxic metabolite. However, in chicken manure systems, degradation efficiency reached a plateau at approximately 20%, likely due to preferential utilization of alternative nitrogen sources and inherent metabolic constraints. These findings highlight the potential of microbial remediation to mitigate cyromazine contamination while underscoring the need for strain optimization and comprehensive ecotoxicological assessments.
研究领域:
环境科学
·
微生物学
·
昆虫学
✓ 已验证
产品关联
DOI
高影响因子
Agriculture-Basel
·
2024
·Vol. 14
·期 6
·881
·
IF 3.3
Ximei Xu,Xiaofeng Yue,Du Wang,Mengxue Fang,Li Yu,Fei Ma,Nanri Yin,Xuefang Wang,Baocheng Xu,Liangxiao Zhang,Peiwu Li
Cadmium is the main heavy metal contaminant of food in the world. The extent of cadmium pollution in peanut in China remains unclear. To determine the cadmium pollution level in peanut, samples from the main producing regions in China were assessed. The findings revealed that the cadmium pollution level in Chinese peanuts was relatively low. Moreover, the Aflatoxin Rhizobia CoupleB. amyloliquefaciens,B. laterosporu,B. mucilaginosus,E. ludwiggi(ARC-BBBE) microbial inoculants on cadmium contamination in peanut were evaluated. The fertilization methods were categorized into conventional fertilization and conventional fertilization supplemented with 60 kg/hectare of microbial inoculant ARC-BBBE as the base fertilizer. The cadmium contents in the soil and peanut plant parts were detected and analyzed. The results demonstrated that the microbial inoculant ARC-BBBE significantly reduced the total cadmium content in peanut, as well as the available cadmium and exchangeable cadmium in soil. Furthermore, the pH and urease and alkaline phosphatase activities in soil were significantly enhanced, suggesting that the microbial inoculant ARC-BBBE decreased cadmium content in soil and reduced the cadmium uptake by plants through a combination of the action of the bacteria itself and the secretion of extracellular substances. This ultimately achieves the goal of reducing the cadmium content in peanut seeds.
研究领域:
环境科学
·
农业
·
微生物学
·
土壤学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Rui Zhou,Dong-Jie Cui,Qi Zhao,Kai-Kai Liu,Wen-Bo Zhao,Qian Liu,Ruo-Nan Ma,Zhen Jiao,Lin Dong,Chong-Xin Shan
The microbial spoilage of soybeans during soaking process severely deteriorates the quality of soybean products and threatens human health. Herein, water-soluble aminated zinc oxide nanoparticles (ZnO NPs) were developed to effectively control the microbial spoilage in soybeans during soaking. ZnO NPs achieved significant inactivation of three dominant spoilage bacteria ( bacillus cereus , bacillus megaterium and enterococcus faecium ) isolated from the deteriorated soybeans, which could adhere to the bacterial surface and damage the cell wall/membrane, but also generate large amounts of reactive oxygen species (ROS). Compared to two commercial ZnO, water-soluble ZnO exhibited superior antibacterial properties due to producing more ROS and bacteria-adhered ability. After ZnO NPs treatment, the content of the residual Zn (51.1 mg/kg) in soybeans was the safety standards of Zn element in soybeans products for human). Therefore, the water-soluble ZnO NPs showed great potentials as efficient and safe antimicrobial agents for soybeans preservation during soaking process.
研究领域:
食品科学
·
微生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2015
·
IF 3.082
Lei Rao,Zhenzhen Xu,Yongtao Wang,Feng Zhao,Xiaosong Hu,Xiaojun Liao
The objective of this study was to investigate the inactivation of the Bacillus subtilis spores by high pressure CO 2 combined with high temperature (HPCD + HT) and to analyze the clumping effect of the spores on their HPCD + HT resistance. The spores of B . subtilis were subjected to heat at 0.1 MPa and HPCD at 6.5–25 MPa, and 82 °C, 86 °C, and 91 °C for 0–120 min. The spores were effectively inactivated by HPCD + HT, but a protective effect on the spores was also found, which was closely correlated to the pressure, temperature and time. The spores treated by HPCD + HT at 6.5 and 10 MPa exhibited a two-stage inactivation curve of shoulder and log-linear regions whereas the spores at 15–25 MPa exhibited a three-stage inactivation curve of shoulder, log-linear and tailing regions, and these curves were well fitted to the Geeraerd model. Approximately 90% of pyridine-2,6-dicarboxylic acid (DPA) was released after HPCD + HT and the 90% DPA release time depend on the pressure and temperature. Moreover, the spore clumping in suspensions was examined by dynamic light scattering. The particle size of the spore suspensions increased with the increase of pressure, temperature and time, indicating the spore clumping. 0.1% Tween 80 as a surfactant inhibited the spore clumping and increased the inactivation ratio of the spores by HPCD + HT. These results indicated that the spore clumping enhanced the spores' resistance to HPCD + HT and induced a protective effect.
研究领域:
微生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Processes
·
2025
·Vol. 13
·期 9
·2854
·
IF 2.8
Min Wang,Chunjing Yu,Xiaoyu Zhao,Junhao Liu,Haochen Zhai,Meng Qi,Xiumei Zhang,Yinsong Liu
Microbial-enhanced oil recovery (MEOR) is a promising technology for oilfield development. To improve MEOR efficiency, two functional strains—Bacillus mucilaginosus ZZ-8 and Bacillus amyloliquefaciens ZZ-11—were isolated and purified. The growth characteristics, biosurfactant production, and crude oil emulsification performance of these strains were systematically evaluated through single-strain cultures and a co-culture system (ZZ-8: ZZ-11 = 1:1). The results demonstrated that the co-culture system exhibited superior growth and functional performance compared to monocultures. The cell-free supernatant significantly reduced oil–water interfacial tension, decreasing the contact angle from 53.56 ± 1.3° to 28.78 ± 0.82°, thereby enhancing crude oil detachment from rock surfaces and improving oil displacement efficiency. Gas chromatography (GC) analysis further confirmed the co-culture system’s pronounced degradation of long-chain alkanes (C17–C35). In oil sand washing experiments, the 1:1 mixed-strain fermentation broth achieved a crude oil elution rate of 84.39%, representing an 89.80% increase over uninoculated medium. This study not only validates the synergistic effect of the B. mucilaginosus–B. amyloliquefaciens co-culture system in enhancing oil recovery but also provides a theoretical foundation and innovative strategy for its practical application in MEOR technology.
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Lu Zhao,Youzhi Zhou,Song Qin,Panpan Qin,Jianlin Chu,Bingfang He
β-Galactosidases can catalyze the hydrolysis of lactose and the synthesis of galacto-oligosaccharides (GOS) from lactose. The catalytic activity of β-galactosidases is usually inhibited by galactose and glucose, which hampers the complete hydrolysis of lactose in food products. In this report, a β-galactosidase (denoted as BMG) from Bacillus megaterium YZ08 without the inhibition by galactose and glucose is the first to our knowledge reported. Efficient secretory expression of BMG was successfully achieved in Bacillus subtilis WB800. With the increasing of galactose and glucose, the activity of BMG dramatically enhanced. With addition of 0.6 M galactose or 1 M glucose, the initial activity to o NPG hydrolysis was increased by 2.1-fold and 4.3-fold, respectively. Moreover, the synthetic rate of galacto-oligosaccharides was enhanced by 1.1-fold and 1.9-fold with the addition of 0.7 M galactose or glucose, respectively. The GOS (278 g/L) from 600 g/L lactose by BMG were efficiently produced within 12 h, and the substrate lactose was completely used up. These results suggest that BMG shows a potential application in the food industry.
研究领域:
生物化学
·
生物技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Ping Zhu,Yan Zeng,Peng Chen,Yan Men,Jiangang Yang,Xiaoping Yue,Jiangang Zhang,Yueming Zhu,Yuanxia Sun
A one-pot two-enzyme reaction system was developed to produce high-fructose syrup (HFS) containing healthy rare sugar d-allulose from Jerusalem artichoke (JA). Inulin in JA was converted through the cascade reaction of a novel exo-inulinase from Bacillus velezensis (BvInu) and D-allulose 3-epimerase from Ruminococcu s sp. (RDAE). BvInu and RDAE were expressed in Bacillus subtilis and successfully secreted into the supernatant, which decrease the production cost and avoid enzyme purification. The optimal temperature and stability of extracellular BvInu significantly increased, thereby promoting the catalytic activity of the two enzymes for the cascade reaction in one pot. Inulin in JA powder was almost completely converted into monosaccharides in the treatment with the optimal ratio of BvInu : RDAE (80 : 40 U/g inulin) at 50 °C for 2 h. The ratio of D-glucose, D-fructose, and D-allulose in the product was approximately 1:3:1, and the yield on JA powder was 67%. The system exhibits potential high-valued application of non-grain crops on producing HFS with D-allulose.
研究领域:
生物技术
·
食品科学
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
He Ying,Huang Ao,Liu Yun
Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, a strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies—including chemical treatment, irradiation, and their combinations—were employed, resulting in four high-activity positive mutants (C-7, I-12, CI 7–12, and IC 13–14). Among these, the CI 7–12 mutant exhibited a significantly higher enzymatic activity, showing a 3.12-fold increase (312.2%) compared to the wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving a PLD activity of 35 U/mL. The enzyme demonstrated stability over a wide temperature range (30–60 °C) and pH range (6–10), with a half-life of 128 days. Kinetic analysis revealed a V max of 20.04 μmol/h and a K m of 7.13 μmol/mL, indicating efficient activity. In bioconversion experiments, the PLD-enriched fermentation broth catalyzed the conversion of PC to PS, achieving a 53.0% conversion rate and a 92.3% selectivity for PS in a two-phase system. These findings expand the potential sources of PLD and underscore its applicability for PS production in biotechnological applications.
研究领域:
微生物学
·
生物化学
·
工业生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Guo-Qiang Li,Zheng-Yang Huo,Qian-Yuan Wu,Yun Lu,Hong-Ying Hu
An ultraviolet (UV)-based advanced oxidation process (AOP) for disinfecting water is introduced in this paper. This study aimed to evaluate the potential of UV light-emitting diodes (UV-LEDs)/chlorine AOP (UV/Cl 2 ) for Bacillus subtilis spore inactivation. Chlorine was combined with 265 and 280 nm LEDs (UV 265 /Cl 2 , UV 280 /Cl 2 ) and investigated. The addition of 4.0 mg/L of free chlorine at pH 7.0 in the presence of 125 mJ/cm 2 of UV irradiation resulted in an additional 1.8-log reduction in UV 265 /Cl 2 and 1.5-log reduction in UV 280 /Cl 2 . There was no observed enhancement in spore inactivation with the addition of a radical scavenger, t-BuOH, which indicated the role of • OH in the synergistic effect. To quantitatively evaluate the synergism, the primary treatment with UV/Cl 2 was followed by further UV or Cl 2 treatment. After UV/Cl 2 pretreatment at different pH levels, the 265 and 280 nm LEDs treatment enhanced an approximate 0.4–0.5-log reduction compared to UV only, and Cl 2 treatment enhanced an approximate 0.7–1.1-log reduction compared to Cl 2 only. In addition, at pH 7.0, in UV 265 /Cl 2 -Cl 2 and UV 280 /Cl 2 -Cl 2 , the inactivation rate constant k increased by approximately 2 and 1.5 times, respectively. The CT for the lag phases ( CT lag ) reduced to approximately 67 and 58%, respectively. Similar results were obtained at pH 7.5 and 8.0, and in the secondary effluent. The synergistic effect on spore inactivation suggested that the pathogen inactivation efficiency of sequential UV and chlorine disinfection processes, which are commonly applied, can be significantly enhanced by adding chlorine prior to UV treatment .
研究领域:
微生物学
·
环境科学
·
水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Xiqian Tan,Anqi Sun,Fangchao Cui,Qiuying Li,Dangfeng Wang,Xuepeng Li,Jianrong Li
Edible film is now a trend in the food packaging industry. In this study, edible films were prepared by adding two Bacillus spp. ( Bacillus amyloliquefaciens Y11 and Bacillus velezensis Y12) to a cassava starch and carboxymethyl cellulose sodium matrix. The structural, physicochemical, and biological characteristics of the film were analyzed, and its application in salmon preservation was explored. The film had a dense structure and no pores, indicating that its polymeric components were compatible with each other. The addition of Bacillus spp. increased the antioxidant activity of the film and its ability to eliminate hydroxyl radicals (84.57% and 91.86%, respectively). The film also showed good antibacterial activity against several pathogens and underwent complete degradation in natural soil within 12 days. The film significantly reduced the total coliform count of salmon and extended its shelf life by 3 days, demonstrating its value as a food-packaging material.
研究领域:
食品科学
·
材料科学
·
微生物学
·
食品包装
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Fermentation-Basel
·
2025
·Vol. 11
·期 3
·119
·
IF 3.3
Liang Wu,Yong Xu
Bacillus subtilis(B. subtilis) has booming application prospects in the feed industry as a value-added probiotic. Considering the intermediate and end saccharides of glucan and xylan digestion during lignocellulose biorefinery processing, the various digested saccharides were detected to disclose the bio-uptake and fermenting characteristics by solo or co-fermentation, especially with respect to five monosaccharides, cellobiose, and functional food additive—xylo-oligosaccharides (XOS).B. subtiliscan directly ferment XOS without xylose, achieving a cell yield of 0.71 g/g, while it hardly utilizes cellobiose except glucose co-fermentation. With a 5 g/L glucose addition and co-fermentation, bacterial cell yield was improved to 0.70 g/g. In total,B. subtilishad a 0.56–0.90 g/g cell yield from corncob-digested saccharides and the highest saccharide-uptaking rate of 98%. This study provides critical insights and experimental data to advance lignocellulosic biorefinery technologies, particularly for probiotics production and simplification of enzymatic hydrolysis, as well as the potential application in high value-added XOS biopurification.
研究领域:
生物工程
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）