JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Wei Zhong,Jin Zou,Yanyu Xie,Jing Yang,Mingfang Li,Shuwu Liu,Yansha Gao,Xiaoqiang Wang,Limin Lu
Herein, a highly sensitive and selective electrochemical sensor for the detection of benomyl (BN), a typical benzimidazole fungicide was proposed based on hierarchical nano-Cu x O decorated MWCNTs-COOH/MXene (nano-Cu x O-MWCNTs-COOH /MXene) composite. The ternary heterostructure presents good electrical conductivity, large specific surface area, and high catalytic activity, exhibiting excellent electrocatalytic activity with enhanced electrochemical signals towards the oxidation of BN. Besides, the embedded nano-Cu x O particles possess strong affinity toward benzimidazole molecules, which could promote the preconcentration of BN, further enhancing the sensitivity, as well as ensuring the high selectivity. Benefitting from the synergistic effect of components in the composite, the developed sensor presents a broad linear range from 10.0 nM to 10.0 μM with a low limit of detection of 3.0 nM (S/N = 3). Meanwhile, the nano-Cu x O-MWCNTs-COOH/MXene electrode also displays good long-term stability and repeatability. Furthermore, this method was successfully applied for the detection of BN in apple samples, illustrating its promising practicability.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 9
·1869
·
IF 4.6
Lili Chen,Shuya Xue,Xin Li,Linbo Deng,Jiapeng Li,Jing Zhou,Yansha Gao,Xuemin Duan,Limin Lu
The development of sensitive and selective methods for detecting pesticide residues has become paramount for ensuring food safety. In this work, a high-performance molecularly imprinted electrochemical sensor based on the composite of Cu-BTC- and FeCo-ZIF-derived N-doped carbon (FeCo@NC), synthesized by pyrolysis and electrodeposition, was developed for Benomyl (BN) detection. The materials were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). In this sensing system, the Cu-BTC/FeCo@NC composite used as the electrode substrate displayed a large specific surface area, high electronic conductivity, and rich active catalytic sites, demonstrating excellent electrocatalytic ability toward BN oxidation. Meanwhile, Cu-BTC, with its abundant surface functional groups, facilitated strong hydrogen bonding interactions with the imprinted template molecule of 3,4-ethylenedioxythiophene (EDOT), promoting the formation of a uniform molecularly imprinted membrane on the substrate material surface. The introduced MIP-PEDOT could enhance the selective recognition and enrichment of the target BN, leading to an amplified detection signal. Thanks to the synergistic effects between Cu-BTC/FeCo@NC and MIP-PEDOT, the proposed sensor achieved a low detection limit of 1.67 nM. Furthermore, the fabricated sensor exhibited high selectivity, reproducibility, and interference resistance in detecting BN. The method has been successfully applied to the determination of BN in vegetable and fruit samples, indicating its potential for use in practical applications.
研究领域:
食品科学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2022
·Vol. 169
·期 4
·047506
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IF 4.386
Lulu Xu,Yao Xiong,Ruimei Wu,Xiang Geng,Minghui Li,Hang Yao,Xu Wang,Yangping Wen,Shirong Ai
An emerging machine learning (ML) strategy for the fabrication of nanozyme sensor based on multi-walled carbon nanotubes (MWCNTs)/graphene oxide (GO)/dendritic silver nanoparticles (AgNPs) nanohybrid and the voltametric determination of benomyl (BN) residues in tea and cucumber samples is proposed. Nanohybrid is prepared by the electrodeposition of dendritic AgNPs on the surface of MWCNTs/GO obtained by a simple mixed-strategy. The orthogonal experiment design combined with back propagation artificial neural network with genetic algorithm is used to solve multi-factor problems caused by the fabrication of nanohybrid sensor for BN. Both support vector machine (SVM) algorithm and least square support vector machine (LS-SVM) algorithm are used to realize the intelligent sensing of BN compared with the traditional method. The as-fabricated electrochemical sensor displays high electrocatalytic capacity (excellent voltammetric response), unique oxidase-like characteristic (nanozyme), wide working range (0.2–122.2 μM), good practicability (satisfactory recovery). It is feasible and practical that ML guides the fabrication of nanozyme sensor and the intelligent sensing of BN compared with the traditional method. This work will open a new avenue for guiding the synthesis of sensing materials, the fabrication of sensing devices and the intelligent sensing of target analytes in the future.
研究领域:
分析化学
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纳米技术
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农业科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-10
·
IF 5.7
Shi Min,Xue Shu-Ya,Peng Guan-Wei,Xu Jing-Kun,Gao Yan-Sha,Liu Shu-Wu,Duan Xue-Min,Lu Li-Min
Graphdiyne (GDY) has attracted a lot of interest in electrochemical sensing application with the advantages of a large conjugation system, porous structure, and high structure defects. Herein, to further improve the sensing effect of GDY, conductive MWCNTs were chosen as the signal accelerator. To get a stable composite material, polydopamine (PDA) was employed as connecting bridge between GDY and MWCNTs-NH 2 , where DA was firstly polymerized onto GDY, followed by covalently linking MWCNTs-NH 2 with PDA through Michael-type reaction. The formed GDY@PDA/MWCNTs-NH 2 composite was then explored as an electrochemical sensor for benomyl (Ben) determination. GDY assists the adsorption and accumulation of Ben molecules to the sensing surface, while MWCNTs-NH 2 can enhance the electrical conductivity and electrocatalytic activity, all of which contributing to the significantly improved performance. The proposed sensor displays an obvious oxidation peak at 0.72 V (vs. Hg|Hg 2 Cl 2 ) and reveals a wide linear range from 0.007 to 10.0 µM and a low limit of detection (LOD) of 1.8 nM ( S / N = 3) toward Ben detection. In addition, the sensor shows high stability, repeatability, reproducibility, and selectivity. The feasibility of this sensor was demonstrated by detecting Ben in apple and cucumber samples with a recovery of 94–106% and relative standard deviations (RSDs) less than 2.3% ( n = 5). Graphical Abstract A sensitive electrochemical sensing platform was reported for benomyl (Ben) determination based on a highly stable GDY@PDA/MWCNTs-NH 2 composite.
研究领域:
电化学
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材料科学
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分析化学
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纳米技术
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Andong Wang,Dongsheng Zou,Zhihong Xu,Bin Chen,Xiaopeng Zhang,Falin Chen,Manyun Zhang
Repeated and high-dose carbendazim applications have caused serious soil carbendazim contamination, and eco-friendly and economical approaches have been suggested to promote carbendazim removal in agricultural soil . Spent mushroom substrate (SMS) is a special recycled resource after harvesting mushrooms and can be utilized in contaminated soil amendment. The SMS application into agricultural soil might increase antibiotic resistance gene abundances, and the health risks of SMS application might be reduced with reasonable management to adjust the related electron transport of soil nitrification or denitrification. In this study, the SMS and nitrification inhibitor dicyandiamide were used to remediate agricultural soil contaminated with the carbendazim, and the carbendazim contents, soil microbial biomass, activities and community and human disease genes were determined. Compared to the control treatment, the combined applications of SMS and dicyandiamide significantly decreased soil carbendazim content by 38.14% but significantly enhanced soil β -glucosidase, chitinase, arylsulfatase, urease and electron transfer system activities. The relative abundances of Proteobacteria and Actinobacteria were increased by 11.0% and 8.2% with the SMS application, respectively. The carbendazim residues were negatively correlated with the soil pH, electron transfer system activities and relative abundances of Proteobacteria and Actinobacteria. The relative abundances of human disease genes were also dramatically increased with the SMS application, but compared to the SMS alone, extra dicyandiamide application significantly reduced the relative abundances of human disease genes in soils. The SMS applications into fungicide-contaminated soils could generate double-edged sword effects of facilitating fungicide dissipation but leading to potential health risk increase, while applying the dicyandiamide with SMS might be an effective strategy to decrease the negative effect of health risk.
研究领域:
环境科学
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土壤学
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微生物学
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生态毒理学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·6857
·
IF 4.927
Min Shi,Guanwei Peng,Shuya Xue,Jingkun Xu,Yansha Gao,Shuwu Liu,Xuemin Duan,Limin Lu
In this work, a two-dimensional leaf-like framework-L embedded electrochemically reduced graphene oxide (ERGO@ZIF-L) was proposed as an outstanding electrode material for the sensitive electrochemical sensing of benomyl (BM). ZIF-L is surrounded by ERGO, which could effectively ensure the stability and dispersion of ZIF-L. With this unique combination, the prepared ERGO@ZIF-L displayed excellent synergistic characteristics with a large surface area, excellent conductivity, plentiful active sites, and high electrocatalytic properties, thus endowing it with high sensitivity for BM determination. The experimental parameters, such as solution pH, material volume, and accumulation time, were optimized. Under optimal conditions, the BM sensor showed a wide linear range (0.009–10.0 μM) and low-limit detection (3.0 nM). Moreover, the sensor displayed excellent stability, repeatability, and reproducibility, and good anti-interference capability. The method was successfully applied to detect BM in real-world samples.Keywords:two-dimensional MOFs;zeolitic imidazolate framework;ERGO;benomyl;electrocatalysts;electrochemical sensor
研究领域:
材料科学
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电化学
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分析化学
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纳米技术
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DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2017
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IF 3.778
Jing Yin,Feilong Zhu,Weiyu Hao,Qi Xu,Jin Chang,Huili Wang,Baoyuan Guo
Acylamino acid chiral fungicides (AACFs) are low-toxicity pesticides and considered as non-carcinogenic chemicals to laboratory animals. Though AACFs have potential toxicological effects on mammals by non-genotoxic mechanisms, the toxicoepigenomics of AACFs has not been documented. In this article, we explored toxiciepigenetics of metalaxyl , benalaxyl and furalaxyl through epigenetics research on lambda DNA under different concentration exposure. The toxicoepigenomic difference of stereoisomers was examined also. Our results showed that AACFs would affect methyltransferase activity resulting in modulating DNA methylation levels and pattern. The LOAEL of R-metalaxyl and S-metalaxyl were 30 mM and 0.3 mM, respectively. The LOAEL of (R, S)-benalaxyl and (R, S)-furalaxyl were 0.3 Mm and 30 mM, respectively. A significant dose-response effect between (R, S)-benalaxyl and global methylation level was observed. Global methylation level was more susceptible to S-enantiomer compared to R-enantiomer, which indicated enantiomers of AACFs have the enantioselectivity in toxiciepigenetics. Moreover, the dependence of the methylation inhibition on the chiral center of metalaxyl may suggest a considerable specificity of the compound of AACFs for DNA methyltransferases . The inhibition effect between R-enantiomer and S-enantiomer of AACFs on DNA methylation levels generated in this study is important for low-toxicity pesticides toxicoepigenomics evaluation.
研究领域:
毒理学
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表观遗传学
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分子生物学
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农药科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.7
Dongfang Guo,Xinyu Zhang,Zizhe Li,Meng Dang,Yexuan Mao,Tong Bu,Dan Hai,Lianjun Song,Xianqing Huang,Xiya Zhang
To avoid false-negative or false-positive results in immunoassays caused by non-uniform antibody cross-reactivity during the detection of the sum of albendazole sulfoxide (ABZSO), albendazole sulfone (ABZSO 2 ), and albendazole-2-aminosulfone (ABZNH 2 SO 2 ) (expressed as albendazole residues), the preparation of a group-specific antibody with homogeneous cross-reactivity is crucial for accuracy. Herein, a novel hapten was designed by introducing a urea structure containing active groups from 5-(propylthio)-1H-benzo[d]imidazole-2-amine. Through monoclonal antibody preparation technology, a broad-spectrum monoclonal antibody 9D9 (mAb 9D9) was prepared with IC 50 values of 0.59, 0.98, 0.41 and 1.13 ng/mL for ABZ, ABZSO, ABZSO 2 , and ABZNH 2 SO 2 , respectively. The ability of mAb 9D9 to uniformly recognize ABZ and its three metabolites was attributed to residues Phe-94 of CDRL3, Glu-103 of CDRH3, and Ser-97 of CDRL3, which formed π-alkyl, π-anion, and hydrogen bonds with the sulfur-containing side chain, benzene ring, and secondary amine on the benzimidazole ring, respectively. Finally, a rapid and accurate lateral flow immunoassay was established for the screening of ABZ and its three major metabolites in animal-derived foods.
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高影响因子
Cell Chemical Biology
·
2026
·Vol. 33
·期 2
·227-240
·
IF 9
Jinyao Chen,Ming Zhang,Yeji Wang,Zhiyu Xie,Fei Yang,Hongjun Yu,Di Zhang,Min Fang,Xiaoyi Luan,Xueyang Jin,Xue Wang,Yanan Qiao,Shicun Zheng,Xiuyun Li,Wenqiang Chang,Hongxiang Lou
The overexpression of drug exporters is a well-known mechanism driving antifungal drug resistance. Here, we investigate that converting the exporter to function as a drug importer provides an alternative approach to combat drug resistance. Using high-throughput screening, we identified quaternary ammonium compounds exhibiting hypersensitivity in Candida albicans strains overexpressing MDR1 . Further experiments involving CRISPR-Cas9-mediated deletion in C. albicans , heterologous expression of Ca Mdr1 in Saccharomyces cerevisiae and construction of Ca Mdr1-containing proteoliposomes confirmed Mdr1 acts as an importer of MKT-077, a potent cyanine derivative highly selective against MDR1 -overexpressing strains. By combining computational simulations and the site-directed mutagenesis, critical residues within the transmembrane domains of Ca Mdr1 involved in MKT-077 import were further identified. Moreover, MKT-077 exhibited therapeutic efficacy in Galleria mellonella infected with Mdr1-overexpressing C. albicans . This work opens an avenue to convert drug resistance into drug sensitivity-a paradigm-shifting strategy in antifungal drug discovery.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yueru Ma,Yuqing Yang,Zhiwei Liu,Hongyu Shi,Zixin Jin,Ziwuzhen Wang,Wei Sheng
High-sensitive fluorescence biomimetic sensor for CBZ is developed. • Ni-MOF is combined with specific adsorption capacity of MIP. • Ni-MOF@MIP probe shows high specificity and sensing sensitivity for CBZ. • Adsorption capacity of Ni-MOF@MIP for CBZ has good stability. A novel fluorescence biomimetic sensor with high selectivity and sensitivity for the detection of carbendazim (CBZ) in fruits, vegetables and grains is established. This sensor is assembled from nickel-based metal-organic framework (Ni-MOF) and molecularly imprinted polymer (MIP). Here, Ni-MOF serves as the fluorescent signal source and carrier, and MIP is coated on the surface of Ni-MOF as a biomimetic recognition element for CBZ to prepare Ni-MOF@MIP probe. The fluorescence of Ni-MOF@MIP will be quenched after adding CBZ, and the fluorescence difference can be applied to realize the quantitative detection of CBZ. The sensor shows wide linear range of 0.01 to 5.00 × 10 3 μg/L ( R 2 = 0.996), and the detection limit (LOD) is 8.4 × 10 −3 μg/L (S/N = 3). The recovery of CBZ in spiked fruits, vegetables and grains has been confirmed by high performance liquid chromatography (HPLC). The fluorescent sensor provides a promising strategy for the detection of CBZ residue in fruit, vegetable and grain samples. Download: Download high-res image (163KB) Download: Download full-size image
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Binbin Zhou,Xinyi Li,Xiaoqi Zheng,Meng Liang,Zitong Yang,An Liu,Liang Chen
A novel electrochemical sensor for highly sensitive and selective detection of carbendazim (CBD) in food was proposed in this study. Initially, a three-dimensional carbon composite material (CCM) was formed by simultaneously modifying reduced graphene oxide and reduced graphene nanoribbons on the glassy carbon electrode (GCE) to enhance sensitivity and serve as a stable sensing platform. Then copper nanoparticles were electrodeposited on CCM/GCE, and cyclic voltammetry scanning was performed in potassium ferrocyanide solution to deposit copper-based Prussian blue analogue on CCM/GCE, further improving the sensitivity of the sensor while giving the ability to self-report. Magnetic molecularly imprinted metal-organic framework materials were then dropped onto the electrode to enhance its detection specificity and adsorption capacity for CBD. The sensor has a wide linear range (0.25 nmol/L ∼ 1000 nmol/L) and a low detection limit (0.08 nmol/L), and the recovery rate of CBD in actual corn flour samples is 99.74 % ∼ 102.58 %.
研究领域:
食品科学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Chengxi Zhu,Dong Liu,Zhi Chen,Libo Li,Tianyan You
We reported a carbon nanohorns/gold nanoparticles composites-based impedimetric aptasensor for carbendazim (CBZ) detection in lettuce and orange juice at picogram levels. The increased electron-transfer resistance, resulting from the formation of CBZ-aptamer complex, was recorded by electrochemical impedance spectroscopy as the aptasensor response for CBZ. Under the optimal conditions, the proposed aptasensor displayed a linear response for CBZ ranging from 1 to 1000 pg mL −1 with a detection limit of 0.5 pg mL −1 . Noteworthy, the as-developed aptasensor displayed the lowest detection limit for CBZ among the previously reported methods. Common pesticides (atrazine, thiamethoxam , etc.) with 100-fold concentration did not interfere the CBZ detection. For CBZ detection in lettuce and orange juice, satisfactory recoveries were obtained with standard addition method. Statistics demonstrated that no significant differences were found between the data provided by standard HPLC-MS reference method and developed aptasensing method in term of accuracy and precision. We believe that the proposed aptasensor possesses a potential application for CBZ monitoring in agricultural product and food.
研究领域:
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）