BIOCHEMICAL SYSTEMATICS AND ECOLOGY
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2025
·
IF 2
Lue Hong,Zerong Wu,Jiahui Gong,Jiahui Ma,Shengzhi Liu
Alocasia cucullata (Lour.) Schott is a member of the Araceae family and is characterized by its unique leaf morphology and vibrant flowers; thus, it is a valuable addition to gardens and interior decor. However, A. cucullata may contain toxic compounds, including calcium oxalate crystals, lectins, and alkaloids, and their distribution within the plant tissues remains poorly understood. In this study, untargeted metabolomics was used to analyse the distribution of the bioactive compounds across the roots, stems, leaves, and guttation of A. cucullata . The toxic effects of these tissues on the MDA-MB-231 human breast cancer cell line were evaluated using the MTT assay. Microscopic techniques were utilized to examine the microstructure of the guttation and stems, and the focus was on the morphology of the calcium oxalate crystals. Liquid chromatography and ELISA kits were used to quantify the calcium oxalate and lectin contents in the various tissues. The results demonstrated that the active constituents, primarily fatty acids, phenylpropanoids, flavonoids, peptides, and alkaloids, were concentrated in the roots. Notably, guttation exhibited no toxicity and lacked both calcium oxalate crystals and significant lectin levels. This research enhances the understanding of the bioactive composition and safety profile of A. cucullata and provides a theoretical foundation for future scientific investigations and safe cultivation, with implications for both scientific and industrial applications.
研究领域:
植物学
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毒理学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
FITOTERAPIA
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2017
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IF 2.698
Ye Liu,Tian Tian,Heng-Yi Yu,Ming Zhou,Han-Li Ruan
An extensive phytochemical study on the stems and leaves of Schisandra viridis A. C. Smith led to the isolation of two novel highly oxygenated schinortriterpenoids, pre-schisanartanin P ( 1 ) and wuweizidilactone Q ( 2 ), together with four known ones ( 3–6 ). Their structures with absolute configurations were established on the basis of comprehensive spectroscopic methods, including HRESIMS, 1D and 2D NMR analyses and ECD experiments. All isolates were evaluated for their in vitro cytotoxicities against HepG2, MCF-7 and HT-29 cancer cell lines, none of them showed significant activities.
研究领域:
植物化学
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药物化学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
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2020
·Vol. 104
·期 2
·479-484
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IF 1.51
Lan Tao,Yu Congcong,Li Ren,Ma Zheng,Xi Xingjun,Chu Qiao
BackgroundSolanesol is an important pharmaceutical intermediate raw material, mainly used to synthesize coenzyme Q10, vitamin K2. It can be found prominent in potato stems and leaves. But now potato stems and leaves are always abandoned or discarded as they are not suitable for use as feed in aquaculture or other purposes. These agricultural waste resources can be reutilized as the corresponding extracts.ObjectiveTo develop a simple and standardized method for the detection of total solanesol in potato leaves and its extracts.MethodsN-hexane was chosen as the extraction solvent for three times in the solanesol extraction from potato leaves. HPLC-MS was used for the detection.ResultsThe LOQ was 0.3 µg/g and the linear range was from 0.1 to 50 µg/mL. The precision and stability were evaluated by the relative standard deviations (RSDs) of three samples (potato leaves, Extract-1, Extract-2) for interday and intraday. The accuracy of the method was evaluated by the recoveries of three different spiked concentrations of solanesol for three samples, and results showed it ranged from 80.7% to 99.0% with RSDs less than 8.7%.ConclusionsThe method we established can provide a simple and standardized way for the extraction and detection of total solanesol.HighlightsThe work laid a foundation for the resource reutilization of potato stem and leaf.
研究领域:
植物化学
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分析化学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
·
2023
·
IF 2
Yinping Li,Qing He,Xiaoqing Zhu,Zhufeng Geng,Yi Wang,Jin Li
Daphne altaica Pall. is an important plant in traditional Chinese medicine. Mineral elements play an important role in the formation of active ingredients in medicinal plants. In this study, 14 mineral elements were quantitatively determined by inductively coupled plasma emission spectrometry using the roots, stems and leaves of D. altaica. The distribution variance of mineral elements between different organs were analyzed by statistical analysis methods such as single factor analysis of variance (ANOVA), correlation analysis (CA), principal component analysis (PCA) and cluster heat map. The results show that the mineral element contents in corresponding parts of D. altaica exhibited significant variation, with the order from highest to lowest being K > Ca > Mg > Fe > Al > Mn > Na > Zn > Sr > Cr > Ni > Cu > Pb > Mo. Among them, the highest content of mineral elements are K, Ca, Mg, Mn, Cu and Mo in leaves, and the stems contains more Zn, Sr, Cr and Ni, while the content of mineral elements Fe, Al, Na and Pb in roots is much higher than the other two organs. The ANOVA revealed significant variations in the mineral element content across different regions of D. altaica (p < 0.05). Based on single factor analysis of variance, different parts of D. altaica had different fingerprint of mineral elements, and the concentration of the same mineral elements in different parts had significant difference (p < 0.05). The principal component analysis (PCA) model showed that the cumulative variance contribution rate of first two principal components was 99.10%, in which Mn, Mg and Ca have higher load values, which is consistent with the results of bi-plot and cluster analysis. These results indicated that there were great differences in absorption and utilization of mineral nutrient elements between roots, stems and leaves of D. altaica, and there was significant correlation among mineral elements, indicating that there was a complex synergistic and antagonistic effect among the elements. At the same time, the discriminant analysis of the characteristic elements provided a scientific basis for the quality control of D. altaica.
研究领域:
植物学
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药用植物研究
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化学分析
✓ 已验证
DOI
高影响因子
PHYTOMEDICINE
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2025
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IF 8.3
Meng Zhang,Rui Liu,Ting Zhou,Yuxin Guo,Hui Li,Wei Wu
Background Chronic fatigue syndrome (CFS) is a crippling illness with limited effective treatment options. Recent findings have suggested that ginseng stem and leaf saponins (GSLS) possess anti-fatigue effects. However, the detailed mechanisms by which GSLS impact CFS have yet to be fully elucidated. Purpose This study aimed to explore the efficacy and underlying mechanisms of GSLS in the treatment of peripheral and central fatigue in mice with CFS. Methods A CFS model was established in male C57BL/6 mice, which were then subjected to prolonged swimming tests. The validity of this model and the efficacy of GSLS were verified using the forced exhaustion swimming test and the Morris water maze test. The anti-fatigue mechanism of GSLS was verified using pathological section observations, biochemical index tests, neurotransmitter analysis, and Ultra-high performance liquid chromatography–high-resolution mass spectrometry (UHPLC-MS) metabolomics analysis. Results GSLS prolonged the swim time-to-exhaustion in CFS mice and reduced escape latency in the water maze, revealing their anti-fatigue and memory-improving effects. GSLS significantly protected both peripheral (liver) and central (hippocampus) tissues, as shown in pathological sections. Biochemical indices suggested that GSLS mitigate peripheral fatigue by reducing energy expenditure and oxidative damage and inhibiting the production of harmful metabolites. Furthermore, GSLS altered the abnormal levels of 14 neurotransmitters in the brains of CFS mice, including seven upregulated and seven downregulated neurotransmitters, highlighting their role in mitigating central fatigue through neurotransmitter regulation. Serum metabolomics analysis identified alterations in 19 metabolites across 11 metabolic pathways, thereby elucidating the potential mechanisms by which GSLS treat CFS. Conclusion GSLS ameliorate damage caused by both peripheral and central fatigue. They also act on multiple pathogenic mechanisms by modulating neurotransmitters and serum biomarkers, making them a potential therapeutic agent for CFS.
研究领域:
药理学
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代谢组学
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2018
·Vol. 98
·期 13
·4885-4894
·
IF 2.379
Lei Deng,Ai-Min Shi,Qiang Wang
INTRODUCTION Peanut stems and leaves (PSL) have traditionally been used as both a special food and a herbal medicine in Asia. The sedative–hypnotic and anxiolytic effects of PSL have been recorded in classical traditional Chinese literature, and more recently by many other researchers. In a previous study, four sleep-related ingredients (linalool, 5-hydroxy-4′,7-dimethoxyflavanone, 2′- O -methylisoliquiritigenin and ferulic acid), among which 5-hydroxy-4′,7-dimethoxyflavanone and 2′- O -methylisoliquiritigenin were newly found in Arachis species, were screened by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS). In the current study, quantitative examination of the above four ingredients was conducted. Serious fundamental functional studies were done in mice, including locomotor activity, direct sleep tests, pentobarbital-induced sleeping time tests, subthreshold dose of pentobarbital tests and barbital sodium sleep incubation period tests, to determine the material base for the sedative–hypnotic and anxiolytic effects of aqueous extracts of PSL. Furthermore, neurotransmitter levels in three brain regions (cerebrum, cerebellum and brain stem) were determined using UHPLC coupled with triple-quadrupole mass spectrometry (UHPLC/QQQ-MS) in order to elucidate the exact mechanism of action. RESULTS Aqueous extract of PSL at a dose of 500 mg kg −1 (based on previous experience), along with different concentrations of the above four functional ingredients (189.86 µg kg −1 linalool, 114.75 mg kg −1 5-hydroxy-4′,7-dimethoxyflavanone, 32.4mg kg −1 2′- O -methylisoliquiritigenin and 44.44 mg kg −1 ferulic acid), had a sedative−hypnotic effect by affecting neurotransmitter levels in mice. CONCLUSION The data demonstrate that these four ingredients are the key functional factors for the sedative–hypnotic and anxiolytic effects of PSL aqueous extracts and that these effects occur via changes in neurotransmitter levels and pathways. © 2018 Society of Chemical Industry
研究领域:
药理学
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植物化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 7
·2114
·
IF 4.927
Yi Li,Xia-Jin Liu,Shu-Lan Su,Hui Yan,Sheng Guo,Da-Wei Qian,Jin-Ao Duan
Present studies have shown that Flos Chrysanthemi has anti-inflammatory and other effects and regulates intestinal function, while the chrysanthemum stem and leaf as non-medicinal parts of chrysanthemum have similar chemical components with chrysanthemum, but the activity and mechanisms are rarely elucidated. Therefore, this study used a DSS-induced zebrafish inflammatory bowel disease model to study the anti-inflammatory and antioxidant effects of chrysanthemum stem and leaf extracts. The results indicate that DSS induction leads to increased secretion of acidic mucin in the intestines of juvenile fish, enlargement of the intestinal lumen and the emergence of intestinal inflammation. Compared with the model group, each administration group differentially inhibited the expression of IL-1β, IL-8 and MMP9 in DSS-induced zebrafish, while upregulating the activity of superoxide dismutase. The quantitative analysis results showed that the flavonoids (including Linarin, Diosmetin-7-glucoside, Tilianin, etc.) and phenolic acids (including Isochlorogenic acid C, Isochlorogenic acid A, 1,3-Dicaffeoylquinic acid, etc.) in the alcohol extract were closely related with both anti-inflammatory and antioxidant activity, while the polysaccharides were also shown a certain anti-inflammatory and antioxidant activity. In conclusion, this study suggests that the flavonoids, phenolic acids and polysaccharides from chrysanthemum stem and leaf extracts can improve inflammatory bowel disease of zebrafish by regulating the expressions of IL-1β, IL-8 and MMP9.Keywords:Flos Chrysanthemi;chrysanthemum stem and leaf;inflammatory bowel disease (IBD);zebrafish;UPLC-TQ/MS
研究领域:
药理学
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炎症研究
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水生毒理学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Lu Liang,Yuchen Liu,Yuxin Liu,Siyi Gan,Xuejin Mao,Yuanxing Wang
The herbal tea made from the leaves and stems of Cyclocarya paliurus is thought to be more flavorful than those made from the leaves only. However, evidence supporting this view is not available yet. 53 VOCs and 54 non-VOCs were identified via HS-SPME-GC-MS and UPLC-Q-TOF/MS, respectively, which were found to vary dramatically in three groups of samples (combination of stems and leaves, separated leaves, and separated stems). 11 VOCs and 18 non-VOCs potential markers are responsible for the change of metabolic effects through the biosynthesis of flavonoids, the synthesis of terpenoids, and the oxidation of fatty acids and carotenoids. In response, the stems were effective to enhance fatty odors, the initial astringency and bitterness and the aftertaste of sweetness of the leaves. Stems are conducive to increasing total phenolic content, total flavonoid content and antioxidant capacity of Cyclocarya paliurus herbal extract because of the increase in polyphenols. In addition, the stems have the potential to be used as raw materials for nerolidol and polyphenols extraction. This study has been the first to determine the contribution of stem to the flavor of Cyclocarya paliurus herbal tea and these results provide the theoretical basis for making Cyclocarya paliurus herbal tea with stems.
研究领域:
植物化学
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食品科学
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代谢组学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2018
·
IF 4.358
Shuyan Zhao,Tao Zhou,Lingyan Zhu,Bohui Wang,Ze Li,Liping Yang,Lifen Liu
N-ethyl perfluorooctane sulfonamide (N-EtFOSA) is an important perfluorooctanesulfonate (PFOS) precursor (PreFOS) which is used in sulfluramid. The present work studied the uptake, translocation and metabolism of N-EtFOSA in wheat ( Triticum aestivum L.), soybean ( Glycine max L. Merrill) and pumpkin ( Cucurbita maxima L.) by hydroponic exposure. Except for parent N-EtFOSA, its metabolites of perfluorooctane sulfonamide acetate (FOSAA), perfluorooctane sulfonamide (PFOSA), PFOS, perfluorohexane sulfonate (PFHxS) and perfluorobutane sulfonate (PFBS) were detected in the roots and shoots of all the three plant species examined. This suggested that plant roots could take up N-EtFOSA from solutions efficiently, and translocate to shoots. A positive correlation was found between root concentration factors (RCFs) of N-EtFOSA and root lipid content. Much higher proportion of N-EtFOSA transformation products in plant tissues than in the solutions suggested that N-EtFOSA could be in vivo metabolized in plant roots and shoots to FOSAA, PFOSA and PFOS, and other additional shorter-chain perfluoroalkane sulfonates (PFSAs), including PFHxS and PFBS. The results suggested that plants had biotransformation pathways to N-EtFOSA that were different than those from microorganisms and animals. This study provides important information about the uptake and metabolism of PreFOSs in plants.
研究领域:
环境科学
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植物生理学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Ying Feng,Bin Li,Juan Hu,Wei Liu,Xufang Tian,Yongxing Chen,Shan Lu,Yifei Liu
The essential oil extracted from the flowers of Chrysanthemum indicum var. aromaticum (CIA) has excellent biological properties; however, the stems and leaves of CIA often get wasted. Maximizing the use of these wastes will help solve the sustainability issues of CIA resources. The present study analyzed the chemical compositions of CIA flower essential oil (CO) and stem and leaf extract (CE) by gas chromatography–mass spectrometry (GC–MS) and ultra-performance liquid chromatography–mass spectrometry (UPLC-MS). Then, the phytochemical properties, stability, antioxidant capacity, and cell (human L929) toxicity of CO and CE were further evaluated. Finally, oil-in-water microemulsion (ME) and hyaluronic acid hydrogel systems composed of CO and CE were developed to deliver bioactive ingredients effectively. The analysis identified 64 and 31 compounds in CO and CE, respectively, and indicated them as readily available sources of natural antioxidants, such as flavonoids and phenols. In addition, the formulation of the ME system (CIA-ME) improved CO stability and increased CE loading; these optimal formulations (ME, CIA-ME, CIA-ME gel) exhibited good stability, rheological properties, and sustained release properties without causing cell toxicity and skin irritation. The CIA-ME significantly inhibited intracellular tyrosinase activity and decreased intracellular melanin production in a dose-dependent manner. It also demonstrated skin photoprotection and inhibited tyrosinase, collagenase, and elastase activities. Overall, CIA as a plant raw material can serve as a promising topical cosmetic or medicine prototype. Applying CO and CE in combination provides a basis for the sustainable utilization of CIA resources.
研究领域:
药学
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化妆品科学
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植物化学
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材料科学
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生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）