DYES AND PIGMENTS
·
2021
·
IF 4.889
Fanyong Yan,Jingru Sun,Yueyan Zang,Zhonghui Sun,Hao Zhang,Jinxia Xu,Xiang Wang
MXene quantum dots (MQDs) derived from MXene with photoluminescent properties have attracted considerable attention. Herein, nitrogen-doped MQDs (N-MQDs) were constructed by a solvothermal method using Ti 3 C 2 MXene as precursors and o-phenylenediamine (oPD) as the nitrogen source. The morphology, element composition and optical properties of N-MQDs were investigated by transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS) and spectroscopy etc. The obtained N-MQDs are dispersed quasi-spherical nanoparticles , mainly composed of carbon, oxygen and nitrogen, with yellow fluorescence at 580 nm and quantum yield of 5.42 %. In further, N-MQDs can detect Alizarin Red (ARS) based on inner filter effect (IFE) with linear range and detection limit of 0–80 μM and 1.21 μM, respectively. N-MQDs can detect ARS in real water samples, and were expected to have further applications in the fields of environmental monitoring and biosensing.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
POWDER TECHNOLOGY
·
2020
·
IF 4.142
Summaira Saghir,Zhenggang Xiao
Metal-organic frameworks (MOFs) are traditional porous materials considered as efficient and low-cost for the removal of organic dyes from polluted water. In this study, we report a new type of hierarchical porous zeolitic imidazole frameworks −67@ layered double hydroxide (ZIF-67@LDH) consisting of nanocubes decorated with nanosheets . The prepared ZIF-67@LDH contains versatile functionalities which exhibit superior adsorption efficiency and adsorption rate for Methyl Orange (MO) and Alizarine Red S (ARS). The influence of pH, temperature, and contact time were analyzed in detail. The kinetics study revealed that the adsorption of MO and ARS over ZIF-67@LDH followed pseudo-second order (PSO) kinetic model , and adsorption isotherms were best described by the Langmuir isotherm model. Adsorption isotherms confirm the monolayer adsorption of MO and ARS over ZIF-67@LDH. The prepared material also shows outstanding recyclability with negligible loss. Thus ZIF-67@LDH presented a promising candidate for treating MO and ARS containing wastewater.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2012
·
IF 4.555
Xi Ba,Liqiang Luo,Yaping Ding,Zhen Zhang,Yuliang Chu,Bijun Wang,Xiaoqian Ouyang
In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results.
研究领域:
生物化学
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分析化学
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材料科学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Dongxia Chen,Jingjin Zhao,Liangliang Zhang,Rongjun Liu,Yong Huang,Chuanqing Lan,Shulin Zhao
Calcium ion (Ca2+) plays crucial roles in the signal transduction pathways associated with various physiological and pathological events. Monitoring intracellular Ca2+ is of great significance for cell biology research. Here, we report the use of biomass quantum dots (BQDs) as a fluorescent reporter for imaging of intracellular Ca2+, based on the inner-filter-mediated luminescence which was assisted by a Ca2+ chelator alizarin red S (ARS). BQDs were prepared by hydrothermal heating of capsicum. The absorption of ARS overlaps with the excitation of the BQDs, the fluorescence of BQDs being quenched through the inner-filter effect. But the absorption of Ca-ARS complex red shifts and shows a poor inner-filter effect. Thus, Ca2+ can be detected by the inner-filter-mediated luminescence using the BQDs-ARS nanohybrid system. Using the proposed nanosystem, the imaging of intracellular Ca2+ and real-time monitoring of the Ca2+ level change under histamine stimulation were also achieved. Thus, this nanosystem holds potential applications in other Ca2+-related signal transduction study.
研究领域:
细胞生物学
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纳米技术
·
生物医学工程
·
荧光成像
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Environmental Science and Technology
·
2023
·
IF 3.1
Yang X.,Mao L.,Shuai H.,Rong Q.,Zhang S.,Lu H.
Fuchsin acid (FA) and other organic dye contaminants have a great harm to the environment and organisms. It is of importance to develop suitable materials with simple preparation process, low cost and excellent performance, for the purification of polluted water. Layered double hydroxides (LDH), as a kind of efficient anion adsorption materials, can be reused simply, but with poor solid–liquid separation efficiency. Herein, the magnetized modified CaAl-LDH (designated as M-CaAl-LDH) was successfully prepared by using cheap compounds such as CaO, AlCl 3 and Fe 2+ /Fe 3+ under the ultrasound-assisted co-precipitation condition. The crystal structure, surface morphology, and elemental compositions were characterized by XRD, FTIR-ATR, SEM–EDS, for the as-prepared LDH materials. The optimized results of removal effect for FA in the simulated sewage show that the removal rate was reached 92.15%, when 0.5 g M-CaAl-LDH was added to the simulated solution with initial pH = 7.5 after adsorption time of 60 min. As for CaAl-LDH before and after magnetic loadings, it is also found that the elimination capacity of M-CaAl-LDH is slightly lower than CaAl-LDH, which may be resulted from the loading of minor amounts of magnetic nano-Fe 3 O 4 with poor removal ability. Graphical Abstract
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Xin Zhang,Youli Wei,Yaping Ding
A glassy carbon electrode modified with poly(alizarin red)/electrodeposited graphene (PAR/EGR) composite film was prepared and applied to detect ciprofloxacin (CPFX) in the presence of ascorbic, uric acid and dopamine. The morphology and interface property of PAR/EGR films were examined by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The electrocatalytic oxidation of CPFX on AR/EGR was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The linearity ranged from 4 × 10 −8 to 1.2 × 10 −4 M with a detection limit (S/N = 3) of 0.01 μM. The modified electrode could be applied to the individual determination of CPFX as well as the simultaneous determination of CPFX, ascorbic acid, uric acid and dopamine. This method proved to be a simple, selective and rapid way to determine CPFX in pharmaceutical preparation and biological media.
研究领域:
分析化学
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电化学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 10
·H425
·
IF 3.12
Chunxiang Chen,Wen Chen,Jinlong Jiang,Lei Qian,Xue Zhang
In this study, nitrofurazone (NF), a commonly used but banned drug in aquaculture, was used as the research model for nitrofuran antibiotics. A poly-alizarin red modified glassy carbon electrode (PAR/GCE) was prepared by electropolymerization. On this modified electrode, NF can produce a sensitive and stable reduction peak. Other nitrofuran antibiotics, such as nitrofurantoin and furazolidone, showed similar responses. The reduction peak of NF was proportional to the concentration within the range of 3.0–50.0 and 50.0–200.0 μM, and the detection limit was 0.33 μM. The linear ranges of furantoin and furazolidone were 10–40 and 50–140 μM, respectively, with corresponding detection limits of 0.73 and 1.56 μM. The modified electrode was used to detect NF in the cultured water sample, and the recovery rate of the sample was 94.5%–104.8%. Thus, the result was satisfactory.
研究领域:
分析化学
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环境科学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2018
·Vol. 124
·期 12
·1-9
·
IF 1.604
Lin Shuang,Lin Xiang,Zhao Haiyan,Hasi Wuliji,Wang Li
Quantitative analysis always encounters difficulty in the field of surface-enhanced Raman spectroscopy (SERS) because of the lack of accurate and reliable analytical means. In this work, a new approach was demonstrated for the quantitative SERS measurement by self-assembled superlattice based on ultra-smooth Au nanospheres (NSs) embedded with 4-methylthiobenzoic acid (4-MBA) as the internal reference. Malachite green (MG) in water and chrysoidine in drinks with detection limit of 0.35 µM were successfully and quantitatively measured by employing Au@4-MBA@Au NSs superlattice as solid SERS substrate. Compared with conventional SERS methods, the fitting linearity of the relative SERS intensity vs. analyte concentrations has significantly improved by this new analysis means. As a result, our approach is a reliable analytical strategy to solve quantitative problems in SERS analysis, which can be easily applied for on-site detection and for preliminary monitoring of food samples.
研究领域:
分析化学
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纳米科学与技术
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食品安全检测
✓ 已验证
DOI
高影响因子
BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-11
·
IF 3.9
Cai Weiye,Sun Bin,Song Chao,Liu Fei,Wu Zhengliang,Liu Zongchao
Background In mouse, it was discovered that resveratrol (Res) enhanced osteoporosis (OP) by boosting osteogenesis. Besides, Res can also have an impact on MC3T3-E1 cells, which are crucial for the control of osteogenesis and thus increase osteogenesis. Although some articles have discovered that Res enhanced autophagy to promote the value-added differentiation of MC3T3, it is unclear exactly how this affects the process of osteogenesis in mouse. Therefore, we will show that Res encourages MC3T3-E1 proliferation and differentiation in mouse pre-osteoblasts and further investigate the autophagy-related mechanism for this impact.Methods (1) MC3T3-E1 cells were separated into blank control group and various concentrations (0.01, 0.1, 1, 10, 100µmol/L) of group in order to determine the ideal Res concentration. In the Res group, Cell Counting Kit-8 (CCK-8) was used to measure the proliferation activity of pre-osteoblasts in mice in each group after resveratrol intervention. Alkaline Phosphatase (ALP) and alizarin red staining were used to gauge the degree of osteogenic differentiation, and RT-qPCR was used to measure the expression levels of Runx2 and OCN in the osteogenic differentiation ability of the cells. (2) In the experiment, four groups were set up: the control group, 3MA group, Res group, and Res + 3MA group. To examine cell mineralization, ALP and alizarin red staining were utilized. RT-qPCR and Western blot detection of cell autophagy activity levels and osteogenic differentiation capacity in each group following intervention.Results (1) Resveratrol might increase the number of mice pre-osteoblast, with the impact being most pronounced at 10µmol/L (P < 0.05). The nodules developed substantially more often than in the blank control group, and Runx2 and OCN expressions significantly increased (P < 0.05). (2) In contrast to the Res group, after 3MA purine blocked autophagy, the Res + 3MA group’s alkaline phosphatase staining and the development of mineralized nodules were reduced. Runx2, OCN, LC3II / LC3I expression decreased, p62 expression increased (P < 0.05).Conclusion The present study partially or indirectly demonstrated that Res may, through increased autophagy, induce osteogenic differentiation of MC3T3-E1 cells.
研究领域:
药理学
·
细胞生物学
·
分子生物学
·
骨骼研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Wei Zhang,Xiaolei Liu,Ping Li,Wen Zhang,Hui Wang,Bo Tang
Elucidating the relationship between glycosylation and phosphorylation of protein post-translational modifications is of great significance for understanding most diseases. Mass spectrometry has been widely used in research of protein phosphorylation and glycosylation. However, to realize in situ and dynamic analysis of the levels of phosphorylation and glycosylation in cells and in vivo, mass spectrometry still has certain difficulties. Herein, a nano-MOF-based fluorescent probe with Zr(IV) and boric acid as the active center was designed and prepared. Fluorescence detection and imaging of phosphate is achieved through the specific interaction of Zr(IV) and phosphate. With aim to achieve specific recognition of glycosylation sites, the boronic acid group was modified in the MOF structure, and the fluorescence of the MOFs was regulated by the alizarin red. Thus, the glycosylation sites were recognized by the competition between alizarin red and glycosyl. Finally, the nanoprobe was successfully applied for in situ fluorescence imaging of the levels of glycosylation and phosphorylation in depressed mice.
研究领域:
生物医学工程
·
分子生物学
·
神经科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2020
·
IF 8.724
Zhenyu Zheng,Taotao Zhou,Rong Hu,Minjun Huang,Xiang Ao,Jun Chu,Tao Jiang,Anjun Qin,Zhongmin Zhang
Osteogenic differentiation is the basis of bone growth and repair related to many diseases, in which evaluating the degree and ability of osteogenic transformation is quite important and highly desirable. However, fixing or stopping the growth of cells is required for conventional methods to monitor osteogenic differentiation, which cannot realize the full investigation of the dynamic process. Herein, a new anion conjugated polymer featuring aggregation-induced emission (AIE) characteristics is developed with excellent solubility for in-situ monitoring the process of osteogenic differentiation. This novel polymer can bind with osteogenic differentiated cells, and the intracellular fluorescence increases gradually with the enhancement of osteogenic differentiation. Moreover, it possesses good biosafety with negligible effect on cell activity and osteogenic differentiation, which cannot be realized by the typical method of Alizarin Red S staining. Further study shows that the polymer crosses the cell membrane through endocytosis and enriches in lysosomes, whereas no obvious fluorescence is detected with other cells, including non-differentiated osteoblast cells, under the same conditions, demonstrating the high selectivity. This is the first fluorescent probe with excellent specificity to realize real-time observation of the process of osteogenic differentiation. Therefore, PTB-EDTA shows great promise in the study of osteogenic differentiation and related applications.
研究领域:
材料科学
·
生物技术
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
·
2022
·Vol. 59
·期 1
·1-9
·
IF 2.723
Chen Sunyu,Xiao Zhanhao,Jiang Wenjin
Osteogenic differentiation is a crucial process of new bone formation. This study aimed to explore the roles and mechanism of SRY-Box Transcription Factor 2 (SOX2) on proliferation and osteogenic differentiation of MC3T3-E1 cells. Bone morphogenetic protein 2 (BMP2) was used to induce the osteogenic differentiation of MC3T3-E1 cells. The expression of SOX2 was determined by quantitative real-time PCR (RT-PCR) at different time points after induction. The SOX2 overexpression plasmids were constructed and transfected into MC3T3-E1 cells. Osteogenic differentiation was evaluated by Alizarin Red S staining and alkaline phosphatase (ALP) assay. The expressions of osteogenic differentiation markers including runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) were detected by western blot assay. Luciferase reporter and CHIP assays were used to confirm that SOX2 regulated the transcriptional activation of leucine-rich repeat-containing G protein–coupled receptor 4 (LGR4). We found that SOX2 was down-regulated upon BMP2-induced osteogenic differentiation in MC3T3-E1 cells. Overexpression of SOX2 effectively inhibited osteogenic differentiation with decreased ALP activity, calcification, and osteogenic differentiation markers’ expression including Runx2, OPN, and OCN. LGR4 was identified as a target of SOX2, and the inhibitory effect of SOX2 on osteogenic differentiation was reversed by knockdown of LGR4. The present study confirmed that SOX2 suppressed osteogenic differentiation of MC3T3-E1 cells through targeting LGR4, which possesses a therapeutic strategy for bone formation and generation.
研究领域:
分子生物学
·
细胞生物学
·
骨骼生物学
·
转录调控
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）