Food Chemistry-X
·
2023
·
IF 6.1
Dan Chen,Ao Wang,Jialiang Lv,Chao Tang,Chang-hai Jin,Jun Liu,Xiaoxiong Zeng,Li Wang
Tea ( Camellia sinensis L.) flower polysaccharides (TFPS) have various health-promoting functions. In the present work, the structure of a purified TFPS fraction, namely TFPS-1-3p, and its in vitro digestive properties were investigated. The results demonstrated that TFPS-1-3p was a typical heteropolysaccharide consisting of rhamnose (Rha), arabinose (Ara), galactose (Gal) and galacturonic acid (GalA) with a molecular weight of 47.77 kDa. The backbone of TFPS-1-3p contained → 4)-α- d -Gal p A(-6-OMe)-(1 → 4)-α-Gal p A-(1 → and → 4)-α- d -Gal p A(-6-OMe)-(1 → 2,4)-α- l -Rha p -(1 → linkages. The branch linkages in TFPS-1-3p contained → 6)-β- d -Gal p -(1→, →3,6)-β- d -Gal p -(1→, →5)-α- l -Ara f -(1 → and → 3,5)-α- l -Ara f -(1 →. Subsequently, TFPS-1-3p could not be degraded under simulated human gastrointestinal conditions but could be of use to human fecal microbes, thereby lowering the pH and increasing the production of short-chain fatty acids (SCFAs) of the gut microenvironment and altering the composition of the gut microbiota. The relative abundance of Fusobacterium_mortiferum Megasphaera_elsdenii_DSM_20460, Bacteroides thetaiotaomicron , Bacteroides plebeius and Collinsella aerofaciens increased significantly, potentially contributing to the degradation of TFPS-1-3p.
研究领域:
食品科学
·
营养学
·
微生物学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Ying Zhou,Qiyuan Peng,Lanting Zeng,Jinchi Tang,Jianlong Li,Fang Dong,Ziyin Yang
After tea leaves, tea ( Camellia sinensis ) flowers are becoming a second tea plant resource because they contain not only functional metabolites similar to those found in tea leaves, but also predominant amounts of functional metabolites that only occur in tea leaves in small amounts. 1-Phenylethanol (1PE) is a predominant aroma compound found in tea flowers. A 1PE synthase in tea flowers was isolated, functionally characterized, and shown to have the highest catalytic efficiency for the conversion of acetophenone (AP). To determine why 1PE accumulates more in tea flowers than other plants, we compared their 1PE contents and used a stable isotope labeling method to elucidate the 1PE biosynthetic route. Supplementation with [ 2 H 8 ] l -phenylalanine and [ 2 H 5 ]AP suggested that most plants containing the enzyme/gene catalyzed the conversion of AP to 1PE. Furthermore, the availability of AP derived from l -phenylalanine was responsible for the difference in 1PE accumulation between tea flowers and other plants.
研究领域:
植物学
·
生物化学
·
植物代谢
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2016
·Vol. 21
·期 4
·1165-1174
·
IF 2.327
Guo Daying,Zheng Cong,Deng Wenjuan,Chen Xi’an,Wei Huifang,Liu Menglan,Huang Shaoming
Nitrogen-doped porous carbon plates have been prepared by simple and cost-effective pyrolysis carbonization of an easily available biomass-fallen camellia flower and followed by alkali activation. As-prepared nitrogen-doped porous carbon (aNPCP3) possesses a high specific surface area of 2318 m 2 g −1 and abundant micro/meso-pores. As a result, the aNPCP3 samples have been demonstrated to be electrodes for supercapacitors, displaying a high specific capacitance of 354 F g −1 at a current density of 0.2 A g −1 and excellent cycling stability. Further, the aNPCP3 samples used as sulfur host materials for lithium-sulfur batteries exhibit a high capacity of 1210 mAh g −1 and good cycling stability with a small capacity decay of about 0.1 % per cycle. Interestingly, it is found that their electrochemical performances are dependent on their specific surface area, pore structure, and heteroatom-doping content and type of carbon materials to a large extent. Cheapness, convenient resource, and good performance make these electrode materials displaying huge potential in cost-effective high-performance energy storage devices.
研究领域:
材料科学
·
能源存储
·
环境科学
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DOI
高影响因子
Food & Function
·
2017
·Vol. 8
·期 12
·4619-4629
·
IF 3.247
Dan Chen,Guijie Chen,Peng Wan,Bing Hu,Ligen Chen,Shiyi Ou,Xiaoxiong Zeng,Hong Ye
In the present study, digestion under saliva, simulated gastric and small intestinal conditions and fermentation in vitro of polysaccharides from the flowers of Camellia sinensis (TFPS) by human gut microbiota were investigated. The results indicated that human saliva and simulated gastric and intestinal juices had no effect on TFPS, while TFPS could be utilized by human fecal microbiota, which was proved from the decreased molecular weight and lower content of total or reducing sugars after fermentation under anaerobic conditions. It was found that pH in the fermentation system decreased, and the production of short-chain fatty acids was significantly enhanced. Furthermore, in vitro fermentation of TFPS altered the composition of gut microbiota, specifically in elevating the ratio of Bacteroidetes to Firmicutes and enriching Prevotella. The present results suggest that TFPS has the potential to be developed as functional foods to modify gut microbiota.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ChemNanoMat
·
2020
·Vol. 7
·期 1
·34-43
·
IF 3.384
Lihua Cao,Huiling Li,Zhaoxiu Xu,Ruirui Gao,Suqing Wang,Guoying Zhang,Shaohua Jiang,Wenhui Xu,Haoqing Hou
Graphical An integrated carbonization, activation, and nitrogen-doped process is applied to fabricate hierarchical porous carbon materials with controllable N content as electrode for supercapacitors. The symmetric supercapacitor delivers an energy density of 13.3 W h kg −1 (6 KOH) and 20 W h kg −1 (1 M Na 2 SO 4 ). Biomass-derived carbon materials are an amazing electrode material for supercapacitor, owing to their abundant, porous structure and composition. Herein, a controllable N content carbon material with hierarchical porous structure was fabricated via an integrated carbonization, activation and nitrogen-doping process. N-rich camellia pollen is used as carbon precursor, while NH 4 Cl is employed as both activation agent and dopant. The optimal N-doped carbon with higher N content (3.82 at%), suitable pore size distribution and larger specific surface area (810 m 2 g −1 ), provides abundant ion transport channels and exposes more accessible active sites. Thus, a high specific capacitance of 280 F g −1 (1 A g −1 ) in 6 M KOH (three-electrode system) can be obtained with making full use of N−HPC-1. Moreover, the assembled symmetrical supercapacitor delivers high energy densities both in 6 M KOH (13.3 Wh kg −1 ) and 1 M Na 2 SO 4 (20 Wh kg −1 ) that can be used for LED lighting. More than that, it is also demonstrates excellent cycle stability in 6 M KOH (85.4% after 20000 cycles at 20 A g −1 ). In view of the above-mentioned merits, this N-doped hierarchical porous carbon is anticipated to be a promising material for application in supercapacitors and other fields.
研究领域:
材料科学
·
能源科学
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产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 51
·32336-32343
·
IF 3.108
Ying Zhou,Lanting Zeng,Yinyin Liao,Fang Dong,Qiyuan Peng,Jianlong Li,Jinchi Tang,Naoharu Watanabe,Ziyin Yang
Insect attacks change the quantity of volatiles emitted from plants, but little is known about the effects on the volatiles' stereochemical configurations. 1-Phenylethanol (1PE) is a major internal volatile of tea (Camellia sinensis) flowers. In the present study, insect-damaged C. sinensis flowers in the field emitted a different (R)-/(S)-1PE pattern than that emitted from undamaged flowers. Laboratory experiments showed that changes in the (R)-/(S)-1PE ratio were caused by treatments with Thrips hawaiiensis (Morgan), a pest of cut flowers and vegetable crops. These treatments led to an increase in jasmonic acid (JA) through the activation of allene oxide synthase, which is involved in JA biosynthesis. Exposing flowers to exogenous JA induced an (R)-/(S)-1PE emission pattern that was similar to that induced by a T. hawaiiensis attack, suggesting that JA was involved in the changes in the stereochemical configuration of 1PE emitted from the flowers after insect treatments. This study provides new insights into the involvement of volatiles in interactions between plants and insects, showing that not only the quantity but also the stereochemical configuration of plant volatiles is affected by insects.
研究领域:
植物学
·
植物-昆虫互作
·
化学生态学
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Bo Lv,Lili Lu,Xuefan Feng,Xiaoping Wu,Xiaoming Wang,Xiong Zou,Fuqin Zhang
Photocatalysis is one of the most promising ways to realize artificial photosynthesis. The biologically inspired photocatalysts with 3D flower-like structures have attracted much attention. In this study, an effective method for the synthesis of composite photocatalytic material, NH 4 TiOF 3 /TiO 2 /g-C 3 N 4 , with a 3D camellia-like structure, was developed. The 3D hierarchical structure of the composite material enabled multiple refractions and reflections of light within the catalyst, which greatly improved the efficiency of the sunlight harvesting. The combination of NH 4 TiOF 3 and TiO 2 also effectively reduced the electron-hole recombination in the g-C 3 N 4 . To evaluate its photocatalytic performance, the prepared nanostructured composite materials were tested for the water-splitting with simulated sunlight. It showed the hydrogen evolution at the rate of 3.6 mmol/g/h, which is 4.0 times faster than that from the pure g-C 3 N 4 . The composite materials exhibited excellent cycling stability. The detailed mechanism of the Z-scheme heterojunction was also discussed. The proposed synthesis route for the creation of 3D flower-like hierarchical composites provides a new effective technique for developing efficient, active, and stable composite photocatalysts for hydrogen production.
研究领域:
材料科学
·
能源化学
·
光催化
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Hongxia Liu,Yingying Liu,Haolei Han,Chengyin Lu,Hongping Chen,Yunfeng Chai
Phenolamides (PAs) are important secondary metabolites present in plants with multiple bioactivities. This study aims to comprehensively identify and characterize PAs in tea ( Camellia sinensis ) flowers using ultra-high-performance liquid chromatography/Q-Exactive orbitrap mass spectrometry based on a lab-developed in-silico accurate-mass database. The PAs found in tea flowers were conjugates of Z/E -hydroxycinnamic acids ( p -coumaric, caffeic and ferulic acids) with polyamines (putrescine, spermidine and agmatine). The positional and Z/E isomers were distinguished through characteristic MS 2 fragmentation rules and chromatographic retention behavior summarized from some synthetic PAs. 21 types of PAs consisting of over 80 isomers were identified, and the majority of them were found in tea flowers for the first time. Among 12 tea flower varieties studied, they all possessed tris -( p -coumaroyl)-spermidine with the highest relative content, and C. sinensis 'Huangjinya' had the highest total relative contents of PAs. This study shows the richness and structural diversity of PAs in tea flowers.
研究领域:
植物化学
·
分析化学
·
茶学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Ying Zhou,Qiyuan Peng,Ling Zhang,Sihua Cheng,Lanting Zeng,Fang Dong,Ziyin Yang
1-Phenylethanol is a chiral flavor compound that has enantiomers, ( R )- and ( S )-1-phenylethanol, with different flavor properties. Given that isolating these enantiomers from plants is low yielding and costly, enzymatic synthesis presents an alternative approach. However, the genes/enzymes that specifically produce ( R )- and ( S )-1-phenylethanol in plants are unknown. To identify these enzymes in tea ( Camellia sinensis ) flowers, 21 short chain dehydrogenase (SDR) genes were isolated from tea flowers, cloned, and functionally characterized. Several recombinant SDRs in Escherichia coli exhibited activity for converting acetophenone to ( S )-1-phenylethanol (CsSPESs, >99.0%), while only one SDR produced ( R )-1-phenylethanol (CsRPES, 98.6%). A pair of homologue enzymes (CsSPES and CsRPES) showed a strong preference for NADPH cofactor, with optimal enzymatic reaction conditions of 45–55 °C and pH 8.0. Identification of the tea flower-derived gene responsible for specific synthesis of ( R )- and ( S )-1-phenylethanolsuggests enzymatic synthesis of enantiopure 1-phenylethanol is possible using a plant-derived gene.
研究领域:
生物化学
·
酶学
·
植物科学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Hao Xue,Tianyi Ma,Luoyilan Zhang,Jing Xiang,Ke Cheng,Hong Zheng,Yin Yuan
In bioanalysis and environmental monitoring, developing sustainable carbon dot nanomaterials from waste biomass as probes holds significant scientific value for advancing sustainable chemical sensing technology. The method not only enables the efficient transformation of waste biomass into functional nanomaterials but also presents a sustainable paradigm for biomass resource utilization. A one-step hydrothermal synthesis protocol was utilized for the fabrication of nitrogen self-doped carbon dots (NCDs), wherein camellia flowers functioned as dual sources of carbon and nitrogen. Furthermore, we constructed a novel chemiluminescence system (Na 2 SO 3 -CeS 2 O 8 -NCDs-H 2 SO 4 ) and applied it to the highly sensitive detection of the antiepileptic drug lamotrigine (LTG) in water. The chemiluminescence signal of LTG exhibited a concentration-dependent inhibitory effect within the system, featuring a linear dynamic range of 8 × 10 -5 M to 1 × 10 -3 M and a detection limit of 2.37 μM. The recovery rates of the target analyte in spiked real water samples ranged from 98.63% to 104.49%, validating the applicability and reliability of this method for detecting LTG in complex environmental matrices. The research offered an innovative approach to the resource utilization of waste biomass and the green analysis of trace drug residues in water samples.
研究领域:
环境监测
·
生物分析
·
纳米材料
·
化学传感
·
绿色化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2018
·Vol. 29
·期 6
·639-648
·
IF 2.337
Yaqian Ma,Menghan Liu,Ting Tan,Aiping Yan,Lan Guo,Kun Jiang,Changheng Tan,Yiqun Wan
Introduction Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. Objective To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection. Methodology Preparing an environmentally-friendly and effective solvent – deep eutectic solvents (DESs) – for compound extraction. Then investigating the influential factors of ultrasound-assisted extraction with DESs by the Box–Behnken design combined with response surface methodology. Results DES-5 synthesised with choline chloride and lactic acid (1:2) acquired excellent extractability for four flavonoids (quercetin 3- O -rhamnoside, kaempferol 3- O -rhamnoside and their aglycones) with different polarity. The proposed method, which could simultaneously determine four flavonoids with HPLC-UV detection for the first time, displays satisfactory recovery yields and high precision with inter-day relative standard deviation lower than 5.80%. Conclusion DESs could be promising solvents for efficiently and selectively extracting bioactive compounds from plant materials, and the analytical method for flavonoids of C. oleifera flowers could provide reference value for its application and be used in other plant resources.
研究领域:
分析化学
·
植物化学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）