JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Qing Shen,Gongshuai Song,Honghai Wang,Yanping Zhang,Yiwei Cui,Hujun Xie,Jing Xue,Haixing Wang
Shrimp waste contains a large number of bioactive lipids for preparing functional foods. In this study, a 1,2-dichloroethane based method for extracting phospholipids from shrimp waste was developed. The number of phospholipids recovered by 1,2-dichloroethane/methanol at a ratio of 1:2 (v/v) was 10.97 g/100 g, which was comparable with the performance of conventional methods. The lipidomics profile of the extract from shrimp waste was studied through gas chromatography with flame ionization detection (GC/FID) and hydrophilic interaction liquid chromatography coupled to QTrap mass spectrometry (HILIC-QTrap/MS) to reveal the effect of different extraction methods on the selectivity of lipids. It was found that the lipid extracts using different methods showed significant variation, while the diversity and percentage of each molecular species were generally the same. Besides, a large number of health beneficial dietary ω-3 polyunsaturated fatty acid (PUFA) structured phospholipids were detected, which indicated that shrimp waste was an important source with significant nutritional values.
研究领域:
食品科学
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生物化学
·
营养学
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产品关联
DOI
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2022
·
IF 3.313
Shiwei Xie,Dan Wei,Yongjian Liu,Lixia Tian,Jin Niu
Dietary fish meal (FM) and fish oil (FO) replacement are crucial for the sustainable development of aquaculture. An eight weeks feeding trial was conducted to evaluate the potential replacing FM with soy protein concentrate , and the suitable FO level for a low FM diet of Penaeus monodon . A diet containing 25% FM was used as the control (C diet), and three other diets were formulated to contain 15% FM, and supplemented with 3%, 2%, 1% FO [high FO (HF), medium FO (MF) and low FO (LF), respectively]. The experiment was conducted in quadruplicates (30 shrimp per replicate, average weight 1.00 ± 0.01 g). The results indicated that besides the final body weight, there were no differences in weight gain, feed efficiency and survival rate observed among the four groups. The results of the acute salinity stress showed that shrimp fed the C and MF diets showed a similar survival rate, which was significantly higher than those fed a LF diet. Shrimp that were fed HF, MF and LF diets showed a lower level of fatty acid transport-related mRNA expression in the hepatopancreas , compared with those fed the C diet. Shrimp fed HF, MF and LF diets showed lower expression levels of superoxide dismutase , endoplasmic reticulum protein 57 and immune deficiency (IMD) in the hepatopancreas than those fed the C diet. Shrimp that were fed a LF diet showed the lowest expression levels of inhibitor of apoptosis proteins, Toll and tumor necrosis factor receptor associated factor 6 (TRAF6) in the hepatopancreas, as well as the highest expression levels of Toll, extracellular signal-regulated kinase, Relish and TRAF6 in the intestine among four groups. Intestinal microstructure damage was observed in shrimp that were fed a LF diet, and intestinal endoplasmic reticulum (ER) swelling was observed in shrimp fed MF and LF diets. In conclusion, these results indicated that the growth performance of shrimp was not affected when the FM and FO contents decreased, whereas a low content of FM negatively influenced the immune response in shrimp by modulating IMD and ER stress related gene expression. Under low FM conditions, a suitable dietary FO level would benefit to the lipid transport, hepatopancreas and intestine health, as well as the immune response of shrimp.
研究领域:
水产养殖
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营养学
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分子生物学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Peng Zhong,Xiaoqun Wei,Yi Xu,Lulu Zhang,Anastasios Koidis,Yunle Liu,Yi Lei,Shaozong Wu,Hongtao Lei
In this work, an untargeted and pseudotargeted metabolomics combination approach was used for authentication of three shrimp species (Litopenaeus vanmamei, Penaeus japonicus, and Penaeus monodon). The monophasic extraction-based untargeted metabolomics approach enabled comprehensive-coverage and high-throughput analysis of shrimp tissue and revealed 26 potential markers. The pseudotargeted metabolomics approach confirmed 21 markers (including 9 key markers), which realized at least putative identification. The 21 confirmed markers, as well as 9 key markers, were used to develop PLS-DA models, correctly classifying 60/60 testing samples. Furthermore, DD-SIMCA and PLS-DA models were integrated based on the 9 key markers, with 59/60 and 20/20 samples of the species that were involved and uninvolved in model training correctly classified. The results demonstrated the potential of this untargeted and pseudotargeted metabolomics combination approach for shrimp species authentication.
研究领域:
食品科学
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代谢组学
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生物化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Le Wang,Mingwu Zang,Xiaoyu Cheng,Shouwei Wang,Xin Zhao,Bing Zhao,Dan Li
This study aimed to explore the effects of storage on lipid profiles changes and potential candidates of lipid oxidation in dried shrimps using chemical and lipidomics analysis. Results showed that peroxide value (POV) and thiobarbituric acid-reactive substances (TBARS) significantly increased. A total of 790 lipid molecular species belonging to 7 main classes were identified, among which glycerophospholipids (GPs, 72.91%) were the most abundant, followed by glycerolipids (GLs, 13.67%) and sphingolipids (SPs, 8.10%). Phosphatidylcholine (PC), phosphatidylethanolamine (PE), triglyceride (TG) and diglyceride (DG) as dominant lipid species were susceptible to oxidization/hydrolyzation mainly because of their unsaturated structures. Using multivariate statistical analysis, 163 differential lipid molecules were screened, including 131 and 32 differentially abundant lipids (DALs) accumulating at high and low abundance levels, respectively. After storage, most of phospholipids significantly decreased, while corresponding lysophospholipids increased. Particularly, it was found that TGs and DGs with fatty acids of 18:1 and 18:2 were more likely to be downregulated. Furthermore, 18 DALs were selected as potential biomarkers of lipid oxidation in dried shrimps. This study provided insights into lipid oxidation mechanism and the changes in lipid profiles of dried shrimps during storage.
研究领域:
食品科学
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生物学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 20
·3286
·
IF 4.7
Di Huang,Chengbin Liu,Huatian Zhou,Xianli Wang,Qicai Zhang,Xiaoyu Liu,Zhongsheng Deng,Danhe Wang,Yameng Li,Chunxia Yao,Weiguo Song,Qinxiong Rao
This study established novel and high-throughput strategies for the simultaneous analysis of 30 fluorinated emerging pollutants in different matrices from the shrimp aquaculture system in eastern China using UHPLC-MS/MS. The parameters of SPE for analysis of water samples and of QuEChERS methods for sediment and shrimp samples were optimized to allow the simultaneous detection and quantitation of 17 per- and polyfluoroalkyl substances (PFASs) and 13 fluoroquinolones (FQs). Under the optimal conditions, the limits of detection of 30 pollutants for water, sediment, and shrimp samples were 0.01–0.30 ng/L, 0.01–0.22 μg/kg, and 0.01–0.23 μg/kg, respectively, while the limits of quantification were 0.04–1.00 ng/L, 0.03–0.73 μg/kg, and 0.03–0.76 μg/kg, with satisfactory recoveries and intra-day precision. The developed methods were successfully applied to the analysis of multiple samples collected from aquaculture ponds in eastern China. PFASs were detected in all samples with concentration ranges of 0.18–0.77 μg/L in water, 0.13–1.41 μg/kg (dry weight) in sediment, and 0.09–0.96 μg/kg (wet weight) in shrimp, respectively. Only two FQs, ciprofloxacin and enrofloxacin, were found in the sediment and shrimp. In general, this study provides valuable insights into the prevalence of fluorinated emerging contaminants, assisting in the monitoring and control of emerging contaminants in aquatic foods.
研究领域:
环境科学
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分析化学
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水产养殖学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Xinghai Huang,Jingjin He,Lu Yao,Ziqi Zhu,Jianwei Luo,Peiyuan Li,Jianhua Wei,Haiyi Zhong
Intelligent food packaging, as an innovative direction for enhancing food quality monitoring capabilities, has garnered extensive attention among consumers and in the research community in recent years. This study constructed composite film materials using sodium alginate (SA) and polyvinyl alcohol (PVA) as the matrix, loaded with the natural active ingredient curcumin to evaluate the potential of the composite film as an indicator of shrimp freshness. The results revealed that the composite film had a thickness of less than 0.1 mm and could effectively block ultraviolet-visible light. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) analyses indicated that curcumin was uniformly distributed within the film matrix. This composite film exhibited strong antioxidant activity (ABTS scavenging rate: 82 %; DPPH scavenging rate: 47.9 %) and reliable pH-responsive characteristics, turning purple or reddish-brown when the pH exceeded 10. This property provides a simple and intuitive method for detecting changes in food quality. In terms of monitoring shrimp freshness, the composite film effectively indicated spoilage. The SA-PVA-CUR30 film changed from light yellow to purple on the fourth day, with a significant increase in the ΔE value (from 34.52 to 51.89), demonstrating optimal indicating performance. In conclusion, this pH-sensitive composite film offers new insights for innovation in food packaging and lays a foundation for the precise control of food quality.
研究领域:
食品科学
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材料科学
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分析化学
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食品安全
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智能包装
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Meiyu Chen,Tianyi Yan,Qinbo Jiang,Jiawen Li,Usman Ali,Yinuo Miao,Shahzad Farooq,Yaqin Hu,Hui Zhang
Electrospun nanofiber films exhibited significant potential as food freshness indicators (FFI) due to their structural advantages. Herein, a novel electrospinning film based on wheat gluten (WG), gelatin, and anthocyanins was fabricated to detect the freshness of shrimp. Compared with pure WG, an adjusted ratio of the WG to gelatin (W16E9, 16:9) improved physicochemical properties, reducing viscosity from 3.56 to 2.75 Pa.s and increasing conductivity from 1.53 to 2.06 mS/cm. SEM showed a transition from bead-like to fibrous morphology (691.6 nm diameter). The films exhibited improved thermal properties, and increased water solubility from 38 % to 45 %. The anthocyanin-enriched film (W16E9A2) maintained a stable purple-red color at −18, 4 and 25 °C for 14 days. It showed sensitivity to acetic acid and ammonia within 40 s, correlating with TVB-N and TVC in shrimp (R 2 = 0.988 and 0.962), distinguishing freshness within 36 h. This work provided a viable protein-based anthocyanin nanofiber film as FFI.
研究领域:
食品科学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yang Yang,Xuena Yu,Yanling Zhu,Yan Zeng,Chunshan Fang,Yu Liu,Shiwei Hu,Yaming Ge,Wei Jiang
To monitor the freshness of Penaeus vannamei during storage, a colorimetric film based on sodium alginate/sodium carboxymethyl cellulose incorporated with rose anthocyanins extract (RAE) was prepared. The results showed that the incorporation of RAE increased moisture content, water vapor permeability, and water contact angle of the colorimetric film. FTIR, XRD spectra, and SEM demonstrated that RAE had good compatibility with the film-forming substrate. The colorimetric film presented obvious color variation in the pH range of 2.0–12.0 and was sensitive to volatile ammonia. The colorimetric film exhibited a visual color change from pink to pale yellow to yellowish green during the storage of Penaeus vannamei at 4 °C. Significant correlations were observed between the color change of colorimetric film (ΔE) and the pH value or TVB-N content of Penaeus vannamei ( p < 0.05). Therefore, the colorimetric film shows great application potential to monitor the freshness of shrimp as intelligent packaging.
研究领域:
食品科学
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包装技术
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Huan Huang,Guocheng Zhou,Zhiyuan Meng,Ting Li,Linfeng Sun,Zhonglong Wang,Yiqin Yang
Biogenic amines (BAs), such as cadaverine (Cad), putrescine (Put), and spermine (Spe), serve as critical indicators of meat freshness. In this study, we developed a novel flavonol-derived fluorescent probe, 4-(3-((4-cyanobenzoyl)oxy)-4-oxo-2-(2,4,6-trimethoxyphenyl)-4H-chromen-7-yl)benzoic acid ( CTBA ). CTBA exhibited a sensitive and selective fluorescence response to BAs, with a pronounced emission peak at 510 nm that intensified proportionally with BA concentration. For Cad, the probe demonstrated a linear detection range of 0–45 μM, achieving a low limit of detection (LOD) of 0.36 μM. The interaction mechanism between CTBA and cadaverine was validated through 1 H NMR and high-resolution mass spectrometry (HRMS). Furthermore, a composite fluorescent film was fabricated by spin-coating CTBA with ethyl cellulose (EC), leveraging hydrogen-bonding interactions. This film was successfully applied for real-time monitoring of freshness in fish, and prawn. Our work introduces a pioneering bifunctional material capable of dual-purpose packaging and freshness detection, offering significant potential for enhancing food safety protocols.
研究领域:
食品安全
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分析化学
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传感器
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材料科学
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生物化学
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产品关联
DOI
高影响因子
CURRENT MICROBIOLOGY
·
2025
·Vol. 82
·期 9
·1-11
·
IF 2.6
Gu Yiwen,Lin Xiu,Lu Yanxu,Fang Shuguang,Hu Kun,Ju Xin,Li Liangzhi,Chen Zhi
The increasing use of antibiotics in shrimp aquaculture has raised concerns regarding ecological safety and the emergence of antibiotic resistance. This study aimed to isolate and characterize probiotic lactic acid bacteria (LAB) strains from the gut of Penaeus vannamei to evaluate their potential as antibiotic alternatives in aquaculture. Two strains, S1 and S2, were identified with 99.79 and 99.51% similarity to Pediococcus acidilactici DSM 20284 and Enterococcus lactis BT159, respectively. Based on minimum inhibitory concentration (MIC) assays, both strains exhibited high sensitivity to the tested antibiotics. They also demonstrated notable acid and bile salt tolerance, with S1 maintaining a survival rate of 30% at pH 5 and S2 achieving 40% survival at pH 6. Under 0.03% bile salt concentration, survival rates were approximately 60% (S1) and 45% (S2), while S1 retained 15% viability even at 0.9% bile salt. Enzymatic activity analysis indicated that both strains exhibited lower amylase activity compared to other hydrolytic enzymes, with S2 showing higher cellulase and protease activities than S1. Antimicrobial assays revealed that both strains exerted broad-spectrum inhibition against four indicator pathogens. S1 exhibited the strongest inhibitory effect against Aeromonas hydrophila (inhibition zone: 1.6–2.2 cm), whereas S2 showed the highest inhibition against Vibrio parahaemolyticus (1.5–2.4 cm). Feeding trials demonstrated that the combined application of S1 and S2 significantly enhanced shrimp body weight (6.05 ± 0.09 g vs. 9.23 ± 0.2 g, P < 0.01) and survival rate (62.49% ± 1.56 vs. 93.78% ± 2.55, P < 0.01). These findings highlight the potential of Lactobacillus strains as probiotics in shrimp farming, offering a sustainable approach to reducing antibiotic dependency and improving shrimp health and productivity.
研究领域:
水产养殖
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微生物学
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应用微生物学
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益生菌研究
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抗生素耐药性
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海洋生物学
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动物营养
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产品关联
DOI
PubMed
高影响因子
Journal of Macromolecular Science Part B-Physics
·
2025
·
IF 1.4
Yaping Huang,Botian Zhu,Xuyang Feng,Hualin Zhang,Haoyou Jiang,Yongfang Qian,Ying Wang
The aim of our research reported in this paper was to develop electrospun (Sc2O3-MgO)/alizarin/polycaprolactone/polyvinylpyrrolidone nanofiber membranes with pH-responsive and antibacterial properties. Scanning electron microscopy results showed that alizarin (ALI) was uniformly distributed in the polycaprolactone/polyvinylpyrrolidone (PCL/PVP) matrix, forming pH-responsive membranes. The X-ray diffractometer (XRD), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and thermal analysis results demonstrated that both ALI and Sc2O3-MgO loadings were effective. Moreover, the addition of 0.16 wt% ALI induced distinct color changes (from pale yellow to purple, pH = 2-12) in the ALI/PCL/PVP nanofiber membranes. This behavior stemmed from the hydroxyl group in ALI forming an intramolecular hydrogen bond with the carbonyl oxygen, enabling proton transfer. Furthermore, the ALI/PCL/PVP membranes containing 2.00 wt% Sc2O3-MgO showed good antibacterial effect against Escherichia coli (E. coli) within 24 h, of what indicating its strong antibacterial activity. In addition, a comprehensive 8-day analysis was conducted on the pH, total volatile basic nitrogen (TVB-N) and total number of live bacteria colonies of the shrimp, as well as the color changes in the film. The experiment proved that the shrimp not covered by the film became highly spoiled on the 4th day, while the shrimp covered with the SAPP-2-3 nanofiber membranes only reached a state of high spoilage on the 6th day. Therefore, the SAPP-2-3 nanofiber membranes can effectively extend the shelf life of the shrimp by 2 days. We believe this study provides a new strategy for the research of multifunctional smart food packaging films.
研究领域:
材料科学
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食品包装技术
·
纳米技术
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高分子科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Cong Liu,Yanli Dong,Junmin Wang,Qianqian Wang,Chun Wang,Qiuhua Wu
Phenolic environmental endocrine disruptors (EEDs) pose significant risks to the reproductive and nervous systems. Herein, we synthesized a magnetic OH-anchored porous polymer (M-Pue@TB-HCP2) by linking natural puerarin with 1,3,5-tris(bromomethyl)benzene for the first time. M-Pue@TB-HCP2 showed high extraction recovery (94 %-99 %) for phenolic EEDs (bisphenol S, bisphenol A, bisphenol B and p -tert-butylphenol) thanks to its amphiphilic sites supplying hydrogen bonding and π conjugation interaction. Moreover, M-Pue@TB-HCP2 displayed excellent reusability and long service life. Using M-Pue@TB-HCP2 as magnetic solid-phase extraction adsorbent, coupled with high-performance liquid chromatography-mass spectrometry, a novel method was established for detecting trace EEDs from shrimps, achieving low detection limit (0.80–5.50 ng g −1 for green shrimp, 1.00–4.00 ng g −1 for white shrimp), good spiked recovery of 80.3 %-117 %, relative standard deviation less than 5.7 %. The method has the merits of high sensitivity, good accuracy and excellent reproducibility, making it suitable for the trace analysis of EEDs in shrimps. This work not only provides a new option for rapid and accurate detection of trace EEDs in shrimps but also explores a new strategy for the preparation of efficacious adsorbents by utilizing sustainable raw materials.
研究领域:
环境科学
·
高分子化学
·
分析化学
·
食品安全
·
内分泌学
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纳米技术
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吸附技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）