Lin Xu,Qin Cai,Xueqin He,Xianda Liu,Guodong Dang,Weifeng Zhao,Changsheng Zhao
The decline of renal function for the patients with end-stage kidney disease (ESKD) leads to the loss of uric acid excretion , which may reduce the prognosis of patients and increase mortality. Although hemodialysis could remove most uric acid , the long-term hemodialysis patients commonly face the problems of exacerbating oxidative stress and subsequent complications such as inflammation effects. In this work, urate oxidase-like (UOD-like) nanoenzyme is added to the blood side of polyethersulfone (PES) hemodialysis membrane by a simple double-casting phase inversion method, aiming to simultaneously achieve antioxidation and scavenge uric acid. The results demonstrate that the Janus PES/UOD membrane exhibits the ability to scavenge RNS (ABTS + , 59.75 %) and ROS (H 2 O 2 , 97.76 %; OH, 74.45 %), while efficiently clears 54.62 % uric acid. In addition, the membrane has good blood compatibility and does not cause hemolysis, complement activation , or coagulation. Furthermore, the membrane exhibits the water flux of 56.83 mL/m 2 ·h·mmHg, the urea clearance ratio of 95.33 %, and the BSA retention ratio of 99 %. Overall, we develop a promising hemodialysis membrane with antioxidant, and it opens a new route to fabricate multifunctional hemodialysis membranes.
Yang Liu,Guiliang Li,Qiu Han,Haibo Lin,Qiang Li,Jing Hua,Fu Liu
Inhibiting thrombosis in extracorporeal dialyzer is predominantly determined by the compatible interface design. It is challenging to develop anticoagulant dialyzers for clinical heparin free dialysis. Herein, we develop an environmentally friendly approach to construct a hydrogel skin composed of poly (sodium alginate-acrylic acid) P (SA-AA) network on polysulfone (PSf) membranes via pre-seeding initiator into membrane matrix and subsequently crosslinking SA and AA in aqueous solution. Physicochemical properties of modified membranes with different SA/AA ratio were characterized by attenuated total reflectance fourier transform infrared spectra (ATR-FTIR) spectrum, proton nuclear magnetic resonance ( 13 C NMR), scanning electron microscopy (SEM) and atomic force microscope (AFM) and contact angle. Antifouling property was demonstrated by proteins adsorption and E. coli adhesion. Hemocompatibility was comprehensively evaluated by hemolysis ratio, anticoagulation activity (APTT and TT), complement activation, platelets activation and contact activation. Anticoagulant dialyzer was fabricated via skin implantation strategy to implement a chronic renal swine dialysis with heparin free administration. Clotting was significantly inhibited during the whole dialysis session, moreover, the dialyzer showed improved toxins clearance, including urea (~185 mL/min), creatine (~153 mL/min), inorganic phosphorous (~120 mL/min) and β 2 -microglobulin (~110 mL/min). The modified membrane bearing abundant carboxylate groups contributes to excellent hydrophilicity and Ca 2+ chelation capability, which is considered to be the predominant anti-clotting mechanism via both intrinsic and extrinsic cascades. The anticoagulant PSf dialyzer demonstrates promising clinical application for heparin free dialysis.
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