JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 39
·期 2
·287-295
·
IF 2.737
Li Wang,Zhong Ping Yao,Ping Li,Si-Bao Chen,Pui-Kin So,Zi-Qi Shi,Bin Hu,Li-Fang Liu,Gui-Zhong Xin
Methods based on triple quadrupole tandem mass spectrometry have been widely used and reported as highly selective and sensitive methods for quantifying substances of herbal medicines. However, most of them were limited to targeted components, due to the difficulties to optimize the multiple reaction monitoring transitions without authentic standards. This study proposed a novel strategy for non-targeted optimization of multiple reaction monitoring method based on the diagnostic ion guided family classifications, tandem mass spectrometry database establishment, and transitions and collision energy screening. Applying this strategy, 59 Fritillaria alkaloids in Fritillariae Ussuriensis Bulbus have been classified, and 51 of these Fritillaria alkaloids were successfully detected by the optimal multiple reaction monitoring method. For semi-quantification, the easy-to-obtain Fritillaria alkaloids of each type, such as verticinone for cevanine type and peimisine for jervine type, were used as the reference standards to calibrate the other Fritillaria alkaloids in the same type. The method was demonstrated a good linearity ( R 2 > 0.998) with satisfactory accuracy and precision, and the lower limits of quantification of verticinone and peimisine were estimated to be 0.076 and 0.216 pg, respectively. In addition, the results suggested that the proposed strategy might obtained high quality metabolomics data in discrimination of Fritillaria unibracteata and Fritillaria ussuriensis .
研究领域:
中药分析
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质谱技术
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药用植物研究
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Feng Pan,Tian-Jiao Su,Yang Liu,Kai Hou,Chen Chen,Wei Wu
Rich polysaccharides were directly observed in the bulbs of Fritillaria unibracteata var. wabuensis (FUW) using the periodic acid–Schiff (PAS) method and microexamination. An acidic water-soluble heteropolysaccharide (FWPS1-1) was isolated from FUW through ethanol precipitation, decoloration, deproteinization, dialysis and separation using a DE-52 anion-exchange column and a Sepharose G-150 gel filtration column. FWPS1-1 (average molecular weight: ~7.44 kDa) has many branches and long side chains; holds the triple-helix conformation; was composed of mannose (Man), galacturonic acid (GalA), galactose (Gal), xylose (Xyl) and arabinose (Ara) with a molar ratio of 2.62:5.59:10.00:0.76:9.38; and features side chains that may be composed of Ara, Man, Gal and GalA, while the backbone may be composed of Xyl, Ara and Gal. In addition, the backbone of FWPS1-1 mainly consists of α-type glycosidic bonds. Bioactivity tests in vitro showed that the polysaccharide exhibited weak 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and low ferric reducing antioxidant power (FRAP) but high 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)l (ABTS) radical scavenging activity, good Fe(II)-chelating ability and remarkable DNA damage protective activity. FWPS1-1 was the first heteropolysaccharide purified from FUW and showed good antioxidant activity and DNA protective effect. The results confirmed that macromolecule is also bioactive ingredient that requires attention like the small-molecule active compounds in FUW.
研究领域:
药用植物学
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生物化学
·
药学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Li Wang,Li-Fang Liu,Jian-Ying Wang,Zi-Qi Shi,Wen-Qi Chang,Meng-Lu Chen,Ying-Hao Yin,Yan Jiang,Hui-Jun Li,Ping Li,Zhong-Ping Yao,Gui-Zhong Xin
In this study, a new strategy combining mass spectrometric (MS) techniques with partial least squares regression (PLSR) was proposed to identify and quantify closely related adulterant herbal materials . This strategy involved preparation of adulterated samples, data acquisition and establishment of PLSR model. The approach was accurate, sensitive, durable and universal, and validation of the model was done by detecting the presence of Fritillaria Ussuriensis Bulbus in the adulteration of the bulbs of Fritillaria unibracteata. Herein, three different MS techniques, namely wooden-tip electrospray ionization mass spectrometry (wooden-tip ESI/MS), ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and UPLC-triple quadrupole tandem mass spectrometry (UPLC-TQ/MS), were applied to obtain MS profiles for establishing PLSR models. All three models afforded good linearity and good accuracy of prediction, with correlation coefficient of prediction ( r p 2 ) of 0.9072, 0.9922 and 0.9904, respectively, and root mean square error of prediction (RMSEP) of 0.1004, 0.0290 and 0.0323, respectively. Thus, this strategy is very promising in tracking the supply chain of herb-based pharmaceutical industry , especially for identifying adulteration of medicinal materials from their closely related herbal species.
研究领域:
药物分析
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中药质量控制
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代谢组学
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分析化学
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天然药物化学
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药物鉴定
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食品与药品安全
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Conghui Zhou,Yuwei Jia,Qi Zhang,Wenhua Huang,Jizhong Yan,Xuhui Ying,Hui Zhang
Ethnopharmacological relevance Pseudobulbus Cremastrae seu Pleiones (PCSP) is a multi-source traditional Chinese medicine (TCM) with diverse chemical compositions and toxicity levels. The authenticity identification and safety evaluation of PCSP have attracted widespread attention in clinical applications. Aim of this study The objective of this study was to evaluate the authenticity and safety of commercially available PCSP. Materials and methods Morphological and microscopic identification, HPLC chromatogram, UPLC-Q-TOF-MS/MS with molecular networking were applied to the authenticity identification of PCSP. The safety of different PCSPs was evaluated by acute toxicity in zebrafish at maximum non-lethal concentration (MNLC) and 10% lethal concentration (LC 10 ). Intestinal toxicity of PCSP was assessed through histological staining, intestinal goblet cells, neutrophils, and intestinal opacity. Results Four sources of PCSP varied in size, epidermal longitudinal grooves, and microscopic features. GNPS analysis identified 61, 47, 44, and 56 chemical compounds in Cremastra appendiculate (CA), Oreorchis patens (Lindl.) Lindl. (OPL), Iphigenia indica A. Gray (IIG), and Tulipa edulis (Miq.) Baker (TEB). Colchicine and militarine, were discovered as distinguishing markers. Acute toxicity in zebrafish ranked as follows: IIG > OPL > CA > TEB. Further studies on the intestinal toxicity of the authentic PCSP (CA, OPL) showed that CA induced less damage with a smaller lumen area, fewer neutrophils and goblet cells, and reduced peristalsis inhibition compared to OPL, indicating greater safety. Conclusion Four different sources of PCSP were accurately distinguished based on three dimensions: character, components, and toxicity. OPL and CA were considered as genuine products, while CA with lower toxicity was more suitable for clinical applications.
研究领域:
药理学
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毒理学
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中药学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2014
·Vol. 30
·期 5
·731-737
·
IF 1.119
Ma Jingwei,Kan Hong,Ma Yinghui,Men Lihui,Pi Zifeng,Liu Zhiqiang,Liu Zhongying
Ultra performance liquid chromatographic-electrospray ionization-mass spectrometry(UPLC-ESI-MS) was used to investigate the potential interaction between selected ingredients of Aconitum and fritillary. The efflux ratios of 14-benzoylmesaconine(BM), 14-benzoylaconine(BC), 14-benzoylhypaconine(BH), mesaconitine(MA), aconitine( AC) and hypaconitine(HA) was 11.16, 12.53, 11.69, 12.8, 11.03 and 6.15, respectively, and the secretion of them was inhibited by Verapamil, which means they are the substrates of permeability-glycoprotein(P-gp). The transport of Aconitum alkaloids extract through a Caco-2 cell monolayer was determined in the absence and presence of fritillary extract. And the fritillary extract increased the absorption of Aconitum alkaloids. Peimine(PE) and peiminine( PEN) in fritillary increased the absorption of pure Aconitum alkaloids. The transport of digoxin was respectively enhanced by PE and PEN, which means they are the inhibitors of P-gp. PEN showed more effective inhibition than PE at the same concentration. The in vitro data suggest that the compounds such as fritillary present in alkaloids were able to inhibit the P-gp activity and lead modifying the transport of alkaloids.
研究领域:
药理学
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中药学
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生物化学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
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2024
·
IF 5.5
Lin-An Cao,Yi-Qian Li,Yun-Fei Huo,Lu Sun,Xiao-Qin Li,Lu Chen,Xue-Tong Yang,Fang-Li Yuan,Ming-Shui Yao
Comprehensive Summary As one of the famous traditional Chinese herbal medicines, Fritillariae Cirrhosae Bulbus (FCB) is widely used in the prevention and treatment of respiratory diseases and has the best curative effect among the known fritillarias medicines. Due to the variety, complex sources, similar appearance and shape, it is difficult to distinguish FCB with high curative effect (h-FCB) from other common fritillarias (c-FCB) in the market. In this paper, a very simple chemiresistor is used to identify FCB from three commonly used fritillarias drugs. The sensors are fabricated by anisotropic electrically conductive metal-organic framework (cMOF) thin film Cu 3 (HHTP) 2 (Cu-HHTP [001] and Cu-HHTP [100] ) as active materials owing to their ability to detect specific groups of volatile organic compounds (volatolomics) as the functional motifs of chemiresistor. As a result, the sensors show orientation-dependence identification ability to FCB. Cu-HHTP [001] - based sensor shows the highest response (344.17%) to 0.5 g h-FCB powder volatiles among its three other c-FCB which is much higher than Cu-HHTP [100] (135.50%). Ultimately, Cu-HHTP [001] can realize the identification of FCB with an accuracy of 97.2% in a simple and real-time manner.
研究领域:
中药学
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传感技术
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植物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xichuang Guo,Keyun Cheng,Chenxin Wang,Jing Zheng,Yeshun Wu,Siwei Wang,Xixi Zeng,Zhujun Mao,Jinyan Gong,Gongshuai Song,Xi Chen,Qing Shen
Fritillaria Bulbus (FB) is a medicinal food homologous substance containing various alkaloids. However, the authenticity and quality control of FB have been regarded as the important and challenging factors in globalization and modernization. In this study, the spatial distributions of alkaloids from four FBs ( Fritillaria thunbergii Miq, FritiLlaria cirrhosa D. Don, Fritillaria ussuriensis Maxim, Fritillaria unibracteata var. wabuensis) were analyzed by a consolidation of UPLC-Q-Extractive Orbitrap MS/MS and direct desorption electrospray mass spectrometry imaging (DESI-MSI). Results showed that 58 reliable metabolites (mainly alkaloids) were identified preliminarily, and taxonomic models were built by chemometric analysis. The alkaloid contents in the bulb base were significantly higher than those in bulb and bulblet ( p < 0.05). Seven metabolites, hexadecanamide, imperialine, L-phenylalanine, jervine, peimine, L-pyroglutamic acid, and trans-3-indolacrylic acid, were screened and confirmed as key characteristic compounds of FB. The detailed spatial information provides a reliable means for rational evaluation of the quality control of FBs. It can better utilize the enriched portion of the alkaloids in FBs. It can provide reference value for the identification of adulteration and development of the medicinal food homologous substance.
研究领域:
药学
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分析化学
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产品关联
DOI
高影响因子
Chinese Medicine
·
2021
·Vol. 16
·期 1
·1-18
·
IF 5.455
Zhou Hongxu,Zhang Yi,Liang Hui,Song Huijie,Zhao Jiaming,Liu Li,Zeng Jun,Sun Lei,Ma Shuangcheng,Meng Dali
Background Belamcanda chinensis (L.) DC. (BC) belongs to the family of Iridaceae and is widely cultivated and used in many Chinese patent medicine and Chinese medicinal formulae. However, due to the high similarities in appearance such as color and shape to Iris tectorum Maxim (ITM), another plant from the same family, BC is often confused or even misused with ITM. Methods Therefore, in order to distinguish the chemical constituents, qualities and biological activities of BC and ITM, multiple technologies including plant metabolomics, digital reference standard (DRS) analyzer and biological activities assay were employed to provide a sufficient basis for their practical applications. Results In plant metabolomics, the PCA and OPLS-DA score plot indicated the obvious differences in chemical profiling between BC and ITM and 6 compounds were successfully identified to contribute to the differences. In DRS study, the fingerprints of 10 and 8 compounds in BC and ITM were developed based on DRS analyzer, respectively, involving relative retention time (RRT) method and linear calibration using two reference substances (LCTRS) technique. The DRS analyzer also accurately identified 10 and 8 compounds from BC and ITM, respectively, by using only two reference standards. In biological activities assay, BC had a better anticancer effect than ITM due to the high abundance of irigenin, while ITM showed stronger hepatoprotective activity than BC because of the high abundance of tectoridin. Conclusions Therefore, due to the significant differences of B. chinensis and I. dichotoma in chemical composition and biological activities, the current studies strongly proved that these two medicinal plants could not be mixed in industrial production and clinical medication.
研究领域:
药用植物研究
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中药质量控制
·
生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuanqi Duan,Zhengyu Hu,Long Jin,Tieqiang Zong,Xiaohui Zhang,Yanan Liu,Pengcheng Yang,Jinfeng Sun,Wei Zhou,Gao Li
The degradation of Belamcanda chinensis (L.) DC. polysaccharides was carried out by five concentrations of trifluoroacetic acid (TFA) (1–5 mol/L), and their physicochemical properties, degradation kinetics and anticomplementary activity were investigated. The findings revealed a notable reduction in the molecular weight of BCP, from an initial value of 2.622 × 10 5 g/mol to a final value of 6.255 × 10 4 g/mol, and the water solubility index increased from 90.66 ± 0.42 % to 97.78 ± 0.43 %. The degraded polysaccharides of B. chinensis exhibited a comparable monosaccharide composition comprising Man, GalA, Glc, Gal, and Ara. As the concentration of TFA increased, the degradation rate constant increased from 1.468 × 10 −3 to 5.943 × 10 −3 , and the process followed the first-order degradation kinetic model (R 2 > 0.97) and the random fracture model (R 2 > 0.96). Furthermore, the five degraded polysaccharides still exhibit good thermal stability. In vitro experiments showed that DBCP-3 exhibited more potent anticomplementary activity than the original polysaccharides and positive drugs, which was strongly correlated with its Mw ( r = 0.6–0.8), inhibiting complement activation by blocking C2 and C4. These results indicated that TFA degradation has a positive effect on polysaccharides, of which DBCP-3 is expected to treat diseases involving hyperactivation of the complement system.
研究领域:
植物化学
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免疫学
·
药理学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Liangli Li,Maohua Yang,Mei Zhang,Mingyan Jia
As an important antitussive and expectorant herbal medicine, Fritillariae cirrhosae bulbus (FCB) is rare and expensive, leading to massive adulterations in the market. Herein, we report a solid colorimetric sensor array (CSA) method for the rapid identification of FCB and its adulterations. This CSA was fabricated by printing nine pH indicators on a mixed cellulose ester membrane via a commercially available inkjet printer filled with polyethylene glycol-containing ink. This CSA displayed color changes to various analytes, as their solution of varied pH values triggers chemical reactions with the nine pH indicators (and other potential interactions). The digital database of these colorimetric response patterns was collected automatically using a homemade program. The outstanding identification capability of the CSA was confirmed via the analysis of 1,2-diols and catechols. The principal component analysis result demonstrated that this CSA could distinguish pure FCB powder from its six common adulterations. The linear discriminant analysis models were established for the qualitative identification of adulterated FCB samples with 89.58 %− 100 % accuracy. Furthermore, partial least squares regression models were developed for the quantitation of adulterant percentages with correlation coefficients higher than 0.87. We expect that the inkjet-printed CSA provided an effective on-site screening method for identifying adulterated FCB.
研究领域:
中药学
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分析化学
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DOI
高影响因子
JOURNAL OF MASS SPECTROMETRY
·
2020
·Vol. 55
·期 9
·e4528
·
IF 1.671
Feng-Jie Liu,Yan Jiang,Ping Li,Yang-Dan Liu,Gui-Zhong Xin,Zhong-Ping Yao,Hui-Jun Li
Fully understanding the chemicals in an herbal medicine remains a challenging task. Molecular networking (MN) allows to organize tandem mass spectrometry (MS/MS) data in complex samples by mass spectral similarity, which yet suffers from low coverage and accuracy of compound annotation due to the size limitation of available databases and differentiation obstacle of similar chemical scaffolds. In this work, an enhanced MN-based strategy named diagnostic fragmentation-assisted molecular networking coupled with in silico dereplication (DFMN-ISD) was introduced to overcome these obstacles: the rule-based fragmentation patterns provide insights into similar chemical scaffolds, the generated in silico candidates based on metabolic reactions expand the available natural product databases, and the in silico annotation method facilitates the further dereplication of candidates by computing their fragmentation trees. As a case, this approach was applied to globally profile the steroidal alkaloids in Fritillariae bulbus, a commonly used antitussive and expectorant herbal medicine. Consequently, a total of 325 steroidal alkaloids were discovered, including 106 cis -D/E-cevanines, 142 trans -D/E-cevanines, 29 jervines, 23 veratramines, and 25 verazines. And 10 of them were confirmed by available reference standards. Approximately 70% of the putative steroidal alkaloids have never been reported in previous publications, demonstrating the benefit of DFMN-ISD approach for the comprehensive characterization of chemicals in a complex plant organism.
研究领域:
中药化学
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代谢组学
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药用植物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Weijie Yang,Shuang Jiang,Chunjuan Yang,Jingnan Chen,Haibo Wu,Sen Li,Yunwei Liu,Xiangquan Li,Zhibin Wang
This study aimed establish a quality control method for Fritillaria ussuriensis Maxim. polysaccharide (FUP). The multidimensional fingerprint of FUP was established, and the relationship between the fingerprint and the antioxidant activity was investigated. The compositions of two monosaccharides (Glc and Ara), five infrared characteristic peaks (1656.12, 1157.89, 1081.38, 1020.03, and 852.14 cm −1 ), two fragments with different molecular weights (1500–500 kDa and < 100 kDa) and four hydrogen signals ( δ 4.98, δ 5.10, δ 5.41, and δ 5.42) were used to evaluate the quality control of FUP. The spectral relationship results showed that the characteristic polysaccharides with higher proportions of Ara and Gal, lower contents of standard normal variable (SNV)1656 cm −1 and SNV 852.14 cm −1 , higher Peak 1, and higher content of δ 5.10 exhibited stronger antioxidant capacity. In conclusion, this study determined the FUP content in F. ussuriensis Maxim. (FU) and established a quality evaluation method based on FUP. Finally, the relationship between the multidimensional fingerprints of polysaccharides and the antioxidant activity was explored, providing a basis for further research on FU.
研究领域:
中药学
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）