MOLECULES
·
2018
·Vol. 23
·期 4
·818
·
IF 3.098
Siyan Zheng,Yingpeng Zhu,Chunyan Jiao,Mengyao Shi,Lianping Wei,Yang Zhou,Qing Jin,Yongping Cai
In order to optimize the extraction of gigantol fromDendrobium officinale, the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.2 mL/g, and the extraction temperature was 41.41 °C. The content of gigantol ofDendrobium officinalein leaves was significantly higher than that in stems, reaching 4.7942 μg/g. The content of gigantol inDendrobium huoshanensisFengdou was significantly higher than that of other species of Fengdou. This experiment has practical significance for improving the utilization rate ofDendrobium officinale, and provides a reference for the study of the pharmacological and biological activity of gigantol.Keywords:Dendrobium officinale;gigantol;response surface methodology;ultrasonic-assisted extraction
研究领域:
植物化学
·
药用植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Chen Xiaoqiang,Yang Shihan,Yang Hong,Zhang Jinshan,Huang Yuyuan,Zhang Ying
Response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) and total phenols (TP) from Berberis kongboensis fruit. Single-factor experiments were conducted with the ultrasonication power, liquid–solid ratio, ethanol concentration, extraction temperature, and extraction time. A Plackett–Burman design was used to identify the main factors affecting the extraction rates of TF and TP. A Box–Behnken design was used to further optimize the ethanol concentration, solvent/sample ratio, and extraction temperature. Under the optimum conditions, the ethanol concentration of 34.67%, solvent/sample ratio of 38.44 mL/g, and extraction temperature of 46.53 °C, the yields of TF and TP were 107.92 mg rutin equivalents/g and 4.86 mg gallic acid equivalents/g, respectively. The extract from UAE had a better yield and higher antioxidant activities in vitro than that obtained by conventional solvent extraction. The B. kongboensis fruit is rich in flavonoids and phenolics, and it has potential application value in the food industry.
研究领域:
植物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Xiao-Qiang Chen,Zi-Han Li,Zhong-Juan Wang,Ling-Ling Liu,Tong-Tong Sun,Jian-Zhang Ma,Ying Zhang
Ultrasound-assisted extraction (UAE) of total anthocyanin (TA) and total phenolics (TP) from Rubia sylvatica Nakai fruit (RSF) was optimized using response surface methodology. On the basis of a single-factor experiment, six factors were screened using a Plackett-Burman experimental design to determine the variables that significantly influence the extraction yield of TA and TP, namely ultrasound power, liquid: solid ratio, ethanol concentration, pH value, extraction temperature, and extraction duration. Based on statistical significance analysis, ethanol concentration, pH value, and extraction temperature were selected for further optimization of the extraction process using a Box–Behnken design. Under the optimum conditions, yields of TA and TP were 22.50 ± 0.89 mg cyanidin-3-glucoside/g RSF and 36.20 ± 0.11 mg gallic acid/g RSF, respectively. The extract prepared under UAE exhibited excellent DPPH radical scavenging activity (IC 50 1.21 ± 0.04 mg/mL). The results demonstrate that R. sylvatica fruit are rich in anthocyanins and show considerable potential as a source of colorants for use in the food industry.
研究领域:
植物学
·
食品科学
·
天然产物化学
✓ 已验证
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Mengyang Hou,Chengyuan Lin,Lin Zhu,Zhaoxiang Bian
This study aimed to enhance the valorization of Chaenomeles speciosa leaves as a sustainable source of bioactive phenolics. An innovative ionic liquid-based ultrasound-assisted extraction (IL-UAE) method was developed for extracting phenolic compounds. Among 10 structurally diverse ILs, [BMIM]Br demonstrated superior extraction performance. Using a combination of single-factor design and response surface methodology (RSM), the optimal parameters for IL-UAE were determined to be the [BMIM]Br concentration of 1.33 mol/L, ultrasonic power of 380 W, extraction time of 10 min, and liquid-to-solid ratio of 22 mL/g. Under these conditions, the yield of C. speciosa leaves total phenolics (CSL-TP) was 78.14 ± 0.35 mg/g, which was substantially higher than those obtained via conventional heat reflux and UAE. After extraction, the microstructures of C. speciosa leaves were examined using scanning electron microscopy (SEM), which confirmed the effectiveness of IL-UAE. Subsequently, NKA-II resin column chromatography was developed to effectively purify crude CSL-TP extracts, guided by leakage and elution curve evaluations, yielding phenolic extracts with a purity of 75.40 % ± 1.93 %. A UPLC–QqQ–MS/MS method was developed for the quantitative analysis of nine major phenolics in purified CSL-TP extracts. Furthermore, bioactivity assessments demonstrated that the purified CSL-TP extracts efficiently scavenged radicals and effectively inhibited the proliferation of HCT-116 and HT-29 cell lines. These results highlight the potential of C. speciosa leaves as a valuable resource for the pharmaceutical and food industries, paving the way for the development of innovative therapeutic products and functional foods.
研究领域:
植物化学
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分析化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2022
·
IF 3.388
Xinyue Pang,Yongshuai Jing,Pengyue Li,Xiaoyue Qiu,Yuguang Zheng,Qian Wang,Lanfang Wu
The study aimed to select the best extraction technology of Angelica dahurica polysaccharides (ADP), characterize its structure, and antioxidant activity. On the basis of the single-factor experiment result, the ultrasonic-assisted extraction (UAE) of ADP was ameliorate using the response surface method. The optimal conditions were: extraction time 32 min, extraction temperature 74°C, ratio of water to material 19 mL/g, extraction power 270 W. The practical extraction rate was 11.21 ± 0.12% (g/g). C-1: Not clear?? g/s??? ADP was purified by column chromatography, and then three polysaccharide fractions (ADP-H, ADP-0.1 and ADP-0.3) were obtained using DEAE-Cellulose 52. The ADP was mainly composed of D-Mannose, D-Glucose, D-Galactose, L-Arabinose, the ADP-H was mainly composed of D-Mannose, D-Glucose, D-Galactose, L-Arabinose, the ADP-0.1 was mainly composed of D-Mannose, D-Galacturonic acid, D-Glucose, D-Galactose, L-Arabinose, and the ADP-0.3 was mainly composed of D-Mannose, L-Rhamnose, D-Galacturonic acid, D-Galactoe, L-Arabinose. The molecular weights of ADP-H, ADP-0.1 and ADP-0.3 were 1.01 × 104 Da, 1.45 × 107 Da and 6.96 × 106 Da. In addition, the purified polysaccharides have certain scavenging ability of DPPH free radical, hydroxyl free radical and ferric reducing power, which can be used as natural products of antioxidants.
研究领域:
植物化学
·
药理学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Yang Yang,Ru Zhao,Hongfei Gao,Zehao Wang,Xinyu Yang,Ming Ruan,Huiyan Gu,Lei Yang,Hao Tian,Changwen Fan,Tingting Liu
In this study, secoiridoids (gentiopicroside, loganic acid, swertiamaroside, and sweroside) were extracted from Gentiana rigescens roots and rhizomes with β-cyclodextrin (β-CD) as the booster and biobased glycine-glycerol deep eutectic solvent (DES) as the extraction solvent and with ultrasound-assisted extraction. The molecular docking performances showed that in DES aqueous solution, β-CD interacts with secoiridoids through noncovalent bonds and hydrogen bonds, and the four secoiridoids studied can be well adapted to the hydrophobic cavity of β-CD. The hydrogen bonds present between secoiridoids and β-CD all reached more than four, and the corresponding binding energies were all lower than − 5.0 kcal/mol. The yields of gentiopicroside, loganic acid, swertiamaroside, and sweroside were 39.82 ± 2.05 mg/g, 6.61 ± 0.34 mg/g, 0.79 ± 0.04 mg/g, and 0.57 ± 0.04 mg/g, respectively, under optimal extraction conditions. The results showed that ultrasound-assisted extraction of secoiridoid compounds in G. rigescens with an aqueous β-CD/DES solution was more effective compared with conventional extraction solvents, which provides a scientific basis for the promising use of β-CD/DES as extraction solvents in natural drugs.
研究领域:
植物化学
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天然药物化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2018
·
IF 1.2
Yifeng Zhou,Liling Wang,Yuchuan Qin,Lichun Chen,Qing Ge,Jianwei Mao
Linderane (LDR) and norisoboldine (NOR) are two typical active compounds in Lindera aggregate (Sims.) Kosterm. In this study, a new method of sequential extraction of LDR and NOR from L. aggregate was developed by supercritical fluid extraction (SFE) and ionic liquid-based ultrasonic-assisted extraction (IL-UAE) for the first time. The suitable SFE with CO2 conditions for LDR were 60 min dynamic extraction time, 40°C temperature and 30 MPa pressure. And the optimal IL-UAE factors for NOR were 2.06 mol/L 1-butyl-3-methylimidazolium bromide ([Bmim] Br) aqueous solution, 44 mL/g liquid–solid ratio, and 67 min ultrasonic time. Compared with the traditional extraction process, the sequential methods not only can obtain higher extraction efficiency, but also can realize the selective extraction for two different kinds of constituents with less consumption of traditional organic solvent. In addition, this environmentally friendly method could be used in a large-scale industry.
研究领域:
植物化学
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中药学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Jiawei Bao,Lu Li,Lanzhen Ban,Xia Pi,Chunmei Li
Bitter apricot kernel (BAK) is a good source of amygdalin and oil. However, the current production process faces challenges including the inability to simultaneously extract amygdalin and oil, and the risk of toxicity due to amygdalin contamination in the oil. A novel, green and efficient ultrasonication-assisted extraction (UAE) method based on a natural deep eutectic solvent (NADES) composed of choline chloride-lactic acid (CC-LA) was established for simultaneous extraction of amygdalin and oil from BAK for the first time. Under optimizing conditions of a liquid-to-solid ratio of 18 mL/g, ultrasonic power of 420 W, temperature of 42 °C, and extraction time of 32 min, the highest yields of amygdalin and oil were achieved at 10.22 ± 0.04 % and 45.64 ± 0.24 %, respectively. The yield of amygdalin in UAE based on CC-LA was twice and nineteen times that of methanol (90 %, v/v) and water, respectively. Meanwhile, the yield of oil obtained by this new method was comparable to that by organic solvent extraction methods. The fatty acid composition of oil obtained by this method was consistent with that by conventional extraction methods. Scanning electron microscopy (SEM) analysis indicated that CC-LA and UAE had a synergistic effect on the destruction of BAK’s microstructure, which facilitated the recovery of amygdalin and oil. Molecular dynamics simulation results showed that amygdalin in CC-LA reached a higher solvent-accessible surface area (SASA) and formed more hydrogen bonds with longer lifetimes and greater stability. Furthermore, this new method demonstrated potential for industrial scalability.
研究领域:
食品科学
·
植物化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Chunlan Sun,Liping Wang,Binjie Zhou,Xialin Jiang,Haiyan Li,Zhangguo Liu
Uncontrolled breeding and improper management of pet cats and dogs have led to the spread of zoonotic Microsporum canis ( M. canis ) infection, which poses challenges to clinical medication. Therefore, there is an urgent need to explore and discover potential fungicides against M. canis . In this study, licochalcone A (LicoA) was prepared by a green and efficient deep eutectic solvent method based on ultrasonic assisted extraction (DES-UAE). The DES-UAE process with choline chloride and propionic acid as solvent was optimized by response surface method with the extraction amount of LicoA as the objective. Following separation and purification, the purity of LicoA reached 99.79 %. Additionally, LicoA demonstrated significant antifungal activity against M. canis with MIC of 4 μg/mL. LicoA inhibited the synthesis of ergosterol in the fungal membrane, destroy the integrity of the cell membrane, lead to the increase of membrane permeability, the release of intracellular electrolytes, the leakage of nucleic acids and proteins, and affect the normal growth of hyphae. ATPase activity was reduced, and mitochondrial membrane potential (MMP) was reduced, resulting in increased levels of ROS, which eventually induced apoptosis in M. canis . Further transcriptome analysis revealed that LicoA treatment affected “cell integrity”, “energy metabolism”, and “oxidative stress” metabolic pathways in M. canis , including cell wall synthesis, ergosterol synthesis, tricarboxylic acid cycle, electron transport chain, and ribosome metabolism, leading to fungal cell apoptosis. These studies establish an efficient method for LicoA extraction and provide a basis for its use as a novel antifungal agent for the treatment of dermatophytosis.
研究领域:
药理学
·
真菌学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 2
·333
·
IF 4.7
Xuejing Jia,Guanghuo Liu,Yun Huang,Zipeng Li,Xiaofei Liu,Zhuo Wang,Rui Li,Bingbing Song,Saiyi Zhong
Alpinia officinarumHance, a well known agricultural product in the Lei Zhou peninsula, is generally rich in polysaccharides. In order to enhance the use ofA. officinarumHance polysaccharides (AOP) in functional food, AOP was extracted using an ultrasonic-assisted extraction method, and the ultrasonic extraction parameters of AOP was optimized. Furthermore, this study investigated the physicochemical and antioxidant activities of AOPs. In addition, the structural properties were preliminarily determined using Fourier-transform infrared spectroscopy (FTIR), high performance size exclusion chromatography, and a Zetasizer. Ultimately, this study explored the mechanism underlying the antioxidant activities of AOP. The results showed that the optimal ultrasonic-assisted extraction parameters were as follows: ultrasonic time, 6 min; ratio of water to material, 12 mL/g; and ultrasonic power, 380 W. Under these conditions, the maximum yield of AOPs was 5.72%, indicating that ultrasonic-assisted extraction technology is suitable for extracting AOPs due to the reduced time and water usage. Additionally, AOPs were purified using graded alcohol precipitation, resulting in three fractions (AOP30, AOP50, and AOP70). AOP30 had the lowest molecular weight of 11.07 kDa and mainly consisted of glucose (89.88%). The half inhibitory concentration (IC50) value of AOP30 and AOP70 was lower than that of AOP50 in the ability to scavenge the ABTS radical, while a reverse trend was observed in reducing ferric ions. Notably, the antioxidant activities of AOPs were highly correlated with their polydispersity index (Mw/Mn) and Zeta potential. AOP30, a negatively charged acidic polysaccharide fraction, exhibited electron donating capacities. Additionally, it displayed strong antioxidant abilities through scavenging 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals and reducing ferric ions. In conclusion, the present study suggests that AOP30 could be developed as an antioxidant ingredient for the food industry.Keywords:Alpinia officinarum;polysaccharide;ultrasonic-assisted extraction;physicochemical characteristics;antioxidant activity
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Zhen Wu,Hong Li,Yongde Wang,Dajian Yang,Hongjun Tan,Yong Zhan,Yong Yang,Yang Luo,Gang Chen
As a discarded byproduct of jujube ( Z . jujuba ), its seeds contain various biological components. Response surface methodology was used to optimize ultrasound-assisted heating extraction conditions of polysaccharides from Z . jujuba cv . Ruoqiangzao seeds (ZJSPs). The optimal parameters were as follows: temperature 83.1 °C, time 100 min, ultrasonic power 140 W, and water-material ratio 33.5 mL/g, allowed a maximum yield of 1.97%. One main fraction (ZJSPs-1) was successfully purified by ion-exchange and gel-permeation chromatography. With a molecular weight of 342 kDa, ZJSPs-1 was composed of arabinose, glucose, xylose, galactose and rhamnose. The presence of pyranose-form sugars as well as both α- and β-configurations was validated by FT-IR and 1 H NMR. TG/DSC indicated that ZJSPs-1 has a favorable thermal stability. XRD suggested that ZJSPs-1 exhibited both crystalline and amorphous portions. ZJSPs-1 was in aggregates of homogenous-dense honeycomb like structures observed by SEM and AFM. ZJSPs-1 possessed antitumor activities against HeLa cells in a dose- and time-dependent manner at 24 and 48 h with IC 50 of 164.6 and 87.1 μg/mL, respectively. Fluorescent microscopic and flow cytometry revealed that ZJSPs-1 inhibited the proliferation of HeLa cells through apoptosis. Z . jujuba seeds are good sources for antitumor polysaccharides in pharmaceutical and food industries.
研究领域:
食品科学
·
药学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Jing Gao,Juyu You,Juhong Kang,Fanghong Nie,Hongwu Ji,Shucheng Liu
In this study, an ultrasonic-assisted extraction using microemulsions was developed for the recovery of natural astaxanthin from shrimp waste. To select applicable solvent systems, the phase equilibrium, microstructure, and physical properties of the microemulsions were investigated. Then the effect of ultrasonic power, ultrasonic time, and microemulsion composition on the extraction efficiency ( EE Ast ) and extraction yield ( E Ast ) of astaxanthin were determined. Compared with organic solvents (ethanol, acetone, and dimethyl sulfoxide), the microemulsion contained tributyloctylphosphonium bromide ([P 4448 ]Br), tributyloctylphosphonium trifluoroacetate ([P 4448 ]CF 3 COO), or tetrabutylphosphonium trifluoroacetate ([P 4444 ]CF 3 COO) resulted in significantly enhanced extraction of astaxanthin due to the stronger electrostatic interactions and hydrogen-bonding interactions. When ultrasonic-assisted extraction conditions were 50 W and 60 min, the highest EE Ast and E Ast reached 32.47 µg·g −1 and 99% by using the IL-in-water microemulsion of [P 4448 ]Br/(TX-100 + n -butanol)/water (0.13:0.25:0.62, w/w), respectively. The IL-based microemulsion is an adequate alternative to conventional methods in the extraction and recovery of astaxanthin from natural bioresources.
研究领域:
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）