NANO
·
2018
·
IF 1.1
Chang Liu,Qianqian Su,Li Li,Jie Sun,Jian Dong,Weiping Qian
In this work, we designed a simple substrate-immersed solvothermal route for the one-step synthesis of novel ordered SiO 2 /Ag arrays, employing SiO 2 colloidal crystals as templates and alcohol as reducing agent. The Ag nanoparticles were uniformly deposited in situ onto SiO 2 colloidal crystals, which exhibited high surface enhanced Raman spectroscopy (SERS) activity and uniform SERS intensity. It was found that ordered SiO 2 /Ag arrays could rapidly scavenge the absorbed-Nile blue A (NBA) molecules from the surfaces with the assistance of H 2 O 2 , while the SERS signals of NBA decreased sharply and almost completely disappeared within four minutes. This can be attributed to the superior catalytic activity of Ag nanoparticles. After five times of re-immersion and re-absorbing process of NBA, the substrates could still keep ∼ 74.8% SERS intensity versus the original. The high activity and durability of the as-prepared SiO 2 /Ag SERS substrate endow them as a promising candidate for trace detection.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Wenya Hou,Wuyong Zhao,Sijing Yi,Wenmei Gao,Jiangang Zhang,Jinzhong Zhao,Xiaoxia Liu
Papain , as a classical cysteine protease , has been widely used in the food, pharmaceutical, chemical, and cosmetic fields. However, there are few information about the peroxidase-like activity of papain catalyzed substrate to produce fluorescence. In this study, we found that papain can catalyze H 2 O 2 to convert o-phenylenediamine (OPD), and generate fluorescence emission at 550 nm under 430 nm excitation. Based on this foundation, we report a papain/OPD/H 2 O 2 system for fluorescence detection of uric acid. The method exhibits a wide linear range of 10–1000 μM with a limit-of-detection of 4.6 μM, and has been successfully used to detect uric acid in human serum. This study paves the way for the application of papain as catalyst for fluorescence detection of different target biomolecules , such as cholesterol, glucose, lactate, for which H 2 O 2 is a product of oxidoreductase enzymes .
研究领域:
生物化学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Qing Li,Run Lu,Huiyu Jiang,Yuyang Zhou
Establishing an energy/water-saving bleaching process is highly expected by textile plants to reduce industrial carbon emission, wastewater discharge and general cost. Therefore, an efficient UVA-assisted N-[4-(triethylammoniomethyl)-benzoyl]- caprolactam-chloride (TBCC) -activated hydrogen peroxide (H 2 O 2 ) bleaching strategy at ambient temperature for cotton/spandex (C/S) fabric was developed in this study. Results reveal that 7.4 g/L of TBCC-activated H 2 O 2 (30%) achieves a slightly higher whiteness index (WI) on C/S fabrics than those only treated with 60 g/L H 2 O 2 (30%). The treated C/S fabric displays a good dyeability using direct dyes due to the qualified wettability. The mild pH process protects C/S fabrics from damage with a low tensile loss (less than 10%) in warp and weft directions. The WI of C/S fabric mainly depends on the concentration of TBCC/H 2 O 2 couple and radiation time. Moreover, the degradation mechanism of cotton pigment in UVA/TBCC/H 2 O 2 bleaching system was implemented including the identification of effective reactive oxygen species (ROS), tracking of the concentration variation of hydroxyl radical (HO•) under UVA irradiation, and the exploration of degrading kinetics and pathway of morin as the model of pigment impurity. Indicated by the degradation rate constant, both TBCC and UVA plays an accelerating role in enhancing the degradation efficiency. In the radical scavenging experiment, HO• and superoxide radical (O 2 – •) are two major ROS participating in the degradation. And therein, HO• makes larger contribution than O 2 – •. HO• concentration has a high positive correlation with radiation duration and TBCC dosage based on the fluorescence labelling method. Confirmed by LC-MS/MS analyses, 2,4,6-trihydroxybenzoic acid (MW: 170.12) and 2,4,-dihydroxybenzoic acid (MW: 154.12) are two major segments degraded from the oxidation and ring-opening of C-ring in morin. Finally, considering energy, water and chemical conservations, the condition i.e. 7.4 g/L of H 2 O 2 (30%) and 20 g/L of TBCC under 30 W UVA radiation for 1 h, is recommended, which significantly increases the WI by 46.5%, and saves about 90% energy and 50% water consumption compared with the conventional waterbath bleaching at 95 °C for 45 min when similar WI enhancement was achieved.
研究领域:
纺织科学与工程
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Yaoyao Zhou,Bo Ling,Hongqi Chen,Lun Wang
A novel fluorescence method for the determination of hydrogen peroxide (H 2 O 2 ) and uric acid (UA) was developed. The procedure was based on the hydroxyl radicals (·OH), which effectively quenched the fluorescence of the Mn 2+ -doped NaYF 4 :Yb,Er upconversion nanoparticles (UCNPs). Based on the property of Mn 2+ -doped NaYF 4 :Yb,Er upconversion nanoparticles, the Fenton reaction and enzymatic reaction of uric acid, this method could be used for highly sensitive detection of H 2 O 2 and uric acid. Under optimal conditions, we observed that the fluorescence quenching signal showed good linearity with the H 2 O 2 concentration in the range of 3.00 × 10 -8 M ~ 6.00×10 -5 M, and the detection limit of this assay was 1.30×10 -8 M. Meanwhile, the linear concentration range for UA was 4.00 × 10 -9 M ~ 1.00×10 -5 M, and the lower limit of detection was 1.90×10 -9 M. Furthermore, the developed method was successfully used for the determination of UA levels in human serum with satisfactory results.
研究领域:
分析化学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Li Wang,Kun Ge,Jiaqi Duan,Xiaomeng Du,Guoqiang Zhou,Lili Ma,Shutao Gao,Jinchao Zhang
Chemodynamic therapy (CDT) based on hydroxyl radicals (•OH) to suppress tumor cells is a promising strategy due to its efficacy and safety. Nevertheless, in tumor cells, CDT still faces challenges such as insufficient •OH and weak killing effect of tumor cells under physiological conditions due to inadequate amounts of endogenous hydrogen peroxide (H 2 O 2 ) and heightened glutathione expression. These challenges limit the therapeutic potential of CDT. To improve the effects of CDT, combination treatment strategies have been developed. Here, we report a rationally designed nanocomposite (CaO 2 @Cu-LA) with self-supplying H 2 O 2 ability from calcium peroxide, and nitric oxide (NO) generation ability from l -arginine. NO molecules not only exhibit a strong killing effect, but also have the potential to transfer into the more cytotoxic substance peroxynitrite anion by reacting with reactive oxygen species. The results showed that CaO 2 @Cu-LA could significantly suppress tumor growth by increasing •OH radicals and NO molecules. Taken together, the strategy developed here provides a good application foreground to yield a remarkable synergistic antitumor effect of CDT and NO gas therapy.
研究领域:
纳米技术
·
癌症治疗
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 12
·期 1
·595-601
·
IF 3.361
Lijuan Chen,Xiang Li,Zezhi Li,Kejian Liu,Jianping Xie
Peroxidases are present widely in microorganisms and plants, and catalyze many reactions. However, the activity of natural peroxidases is susceptible to external conditions. We prepared carbon nanoparticles (CNPs) using an environmentally friendly and simple method. These CNPs were demonstrated to possess intrinsic peroxidase-like activity. CNPs could catalyze the reaction of a peroxidase substrate, 3,3,5,5-tetramethylbenzidine (TMB), in the presence of H2O2 to produce a blue solution at 652 nm. CNPs exhibited higher peroxidase activity than that of other carbon-based nanomaterials. Moreover, CNPs retained their high peroxidase activity after being reused several times. Glutathione (GSH) can change the blue color of oxidized TMB into a colorless hue at 652 nm. Based on this fact, qualitative and quantitative approaches were employed to detect GSH using a colorimetric method. This method showed a broad detection range (2.5–50 μM) with a limit of detection of 0.26 μM. This method was shown to be accurate for GSH detection in a cell culture medium compared with that using a commercial assay kit. Our findings could facilitate application of CNPs in biomedical areas.
研究领域:
纳米技术
·
生物化学
·
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Mengjie Zhang,Zixuan Chen,Haixin Qin,Xiaoyan Yang,Wei Cao,Shufeng Liu
Abtract Heme binds to amyloid β -peptide (A β ) in the brain of Alzheimer΄s disease (AD) patients, thus forming heme-A β complexes and leading the characteristic pathological features of AD. The heme-A β complexes reduce O 2 by 2e − to obtain co-reactant H 2 O 2 . In this work, two-dimensional graphite-like carbon nitride (g-C 3 N 4 ) was hybridized with heme via π-π interaction to construct an electrochemiluminescence (ECL) biosensor for detection of A β peptides. The capture aptamer was firstly immobilized on the gold electrode, and then incubated with target A β peptides and g-C 3 N 4 -heme. The g-C 3 N 4 -heme-A β composite was subsequently anchored on the electrode and showed a signal-on, label-free ECL emission based on dual co-reactants enhancement attributed to in-situ generated H 2 O 2 and original K 2 S 2 O 8 in PBS buffer. Under the optimum experimental conditions, the ECL biosensor has a sensitive response to A β peptides with a linear range of 10 fM-0.1 μM and a detection limit of 3.25 fM. Considering prominent specificity, reproducibility, and stability, the proposed biosensor was used to assess recovery of A β peptides in human serum and the result was satisfactory. We believe that the established method would offer a useful analytical means for quantifying A β peptides in a biological matrix.
研究领域:
生物化学
·
分析化学
·
神经科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Inorganic Chemistry Frontiers
·
2022
·Vol. 9
·期 11
·2544-2556
·
IF 7.779
Xuwen Da,Zhanhua Wang,Yao Jian,Chao Zhang,Yuanjun Hou,Yishan Yao,Xuesong Wang,Qianxiong Zhou
The bottleneck problems of chemodynamic therapy (CDT) are the dependence on an acidic pH (2.0–4.0), insufficient H2O2 level in tumor tissues, and potential systematic toxicity of inorganic nanomaterials-based CDT agents. In this study, the new tricks of two old compounds, i.e. [Ru(phen)2(PIP-OCH3)](PF6)2 (Ru3, phen = 1,10-phenanthroline, PIP-OCH3 = 2-(4-methoxy phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline) and Fe(tpy)Cl3 (tpy = 2,2′:6′,2′-terpyridine), were harnessed to construct an ideal CDT system. Ru3 was able to efficiently photo-catalyze NADH oxidation accompanied by the production of H2O2in situ with a high yield of 73.2%, which can be converted into cytotoxic hydroxyl radicals by the biocompatible Fe(tpy)Cl3 at a neutral pH. By loading both Ru3 and Fe(tpy)Cl3 in tumor-targeting bovine serum albumin (BSA), the obtained BSA@Fe/Ru nanoparticles exhibited a highly efficient, selective, and photo-controlled CDT activity both in vitro and in vivo. This work may provide new sights for overcoming the issues confronted in CDT.
研究领域:
纳米技术
·
癌症治疗
·
光催化
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Beibei Zhang,Hao Liu,Yifei Wang,Yong Zhang
Ferroptosis is an appealing cancer therapy strategy based on the H2O2-involved Fenton reaction to produce toxic •OH for lipid peroxidation. However, intracellular H2O2 is easily consumed and results in a deficient Fenton reaction. This obstacle can be overcome by traditional chemotherapeutic drugs for H2O2 supplements. Moreover, a recent work illustrated that dihydroartemisinin (DHA) could promote ferroptosis against tumoral cells, particularly in the presence of ferrous compounds. To achieve combined chemotherapy and ferroptosis, a nanocarrier (TKNPDHA-Fc) was constructed by using thioketal (TK)-bridged paclitaxel prodrug (PEG-TK-PTX) and ferrocene (Fc)-conjugated PEG-Fc, where DHA was encapsulated by a hydrophobic–hydrophobic interaction. Upon cellular uptake, TKNPDHA-Fc could facilitate PTX release through TK breakage under an excess H2O2 microenvironment. Owing to the loss of the hydrophobic PTX component, TKNPDHA-Fc underwent a rapid dissociation for improving DHA to act as a ferroptotic inducer along with Fe supplied from Fc. Moreover, both the chemotherapy-induced reactive oxygen species and the •OH produced from reinforced ferroptosis further stimulated the TK cleavage. The “self-catalytic” loop of TKNPDHA-Fc remarkably improved the antitumor performance in vivo via combined mechanisms, and its tumor inhibition rate reached 78.3%. This work highlights the contribution of ROS-responsive and self-catalytic nanoplatforms for enhancing the potential of combined chemotherapy and ferroptosis for cancer therapy in the future.
研究领域:
癌症治疗
·
纳米技术
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2024
·
IF 6.7
Xiuwen Gao,Woding Deng,Jinhui Tan,Xiong Shuai,Jun Zan,Tailai Ye,Kaiwu Luo,Fangwei Qi,Yujun Wei,Cijun Shuai
Thermoelectric material could realize the thermal-electricity-reactive oxygen species (ROS) conversion under temperature gradient, being considered a promising candidate for tumor therapy. Nevertheless, it required exogenous excitation such as light irradiation, causing difficultity in achieving on-demand therapy. Herein, a tumor microenvironment responsive Bi 2 Te 3 –CaO 2 nanohybrid was synthetized by in situ growing calcium peroxide (CaO 2 ) nanoparticles on thermoelectric bismuth telluride (Bi 2 Te 3 ) nanosheets, and then blended with poly- l -lactic acid (PLLA) to fabricate a PLLA/Bi 2 Te 3 –CaO 2 scaffold. Once the acidic microenvironment of the tumor was sensed, the CaO 2 would serve as an intelligent switch, releasing heat through rapid hydrolysis and triggering the thermoelectrocatalysis of Bi 2 Te 3 to generate ROS, thereby achieving on-demand therapy. Results demonstrated that the scaffold exhibited rapid temperature rise and significant ROS catalytic formation ability in an acidic solution (pH 5.5), while no significant changes occurred in a neutral environment (pH 7.4). Annexin V-FITC/PI staining and JC-1 assay demonstrated that the generated ROS could induce tumor apoptosis by reducing mitochondrial membrane potential. Besides, the fluorescent probe test revealed that CaO 2 could also produce hydrogen peroxide (H 2 O 2 ) and calcium ions (Ca 2+ ) to interfere with intracellular Ca 2+ , thereby further enhancing the antitumor effect. Overall, this work proposed a novel perspective for on-demand antitumor therapy.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Wenning Yang,Jia Li,Mingyue Wang,Xiaofan Sun,Yong Liu,Jie Yang,Dickon H.L. Ng
A novel core-shell Fe 3 O 4 /CoFe-LDH (layered double hydroxides) hybrid as a peroxidase mimic for the colorimetric detection of ascorbic acid was first fabricated via a facile two-step route. The resulting Fe 3 O 4 /CoFe-LDH hybrid exhibited much higher peroxidase-like catalytic activity for the oxidation of 3,3′,5,5′- tetramethylbenzidine (TMB) with H 2 O 2 than the pristine Fe 3 O 4 and CoFe-LDH nanosheets owing to the unique hierarchical architecture containing more exposed active sites and the synergistic effect between Fe 3 O 4 and CoFe-LDH. A sensitively and selectively visual sensor for the determination of ascorbic acid (AA) was successfully constructed based on the reduction effect of AA with enediol group on the formed oxidation of TMB, which exhibited a sensitive response to AA in the range of 0.5 ∼ 10 μM with the detection limit of 0.2 μM. Additionally, the Fe 3 O 4 /CoFe-LDH magnetic hybrid could be easily recycled by applying a magnetic field. This work provided a feasible means for the fabrication of magnetic nanomaterials with encouraging prospect in biosensing, environmental monitoring and medical diagnostics.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 31
·4014-4021
·
IF 2.378
Qiang Li,Feng Wu,Mao Mao,Xiang Ji,Luyao Wei,Jieying Li,Lan Ma
In this study, water-soluble citrate-capped copper nanoparticles (Cu NPs) were synthesized by a simple and rapid method. We found that these citrate-capped Cu NPs possessed an intrinsic peroxidase-like activity, which could catalyse the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a light blue product in the presence of hydrogen peroxide (H2O2). It was also found that mercury ions (Hg2+) could enhance the peroxidase-like activity of the citrate-capped Cu NPs, causing the colour to turn bright blue. The colour change was dependent on the concentration of Hg2+. Therefore, a colorimetric method for Hg2+ detection was established with a linear range from 0.050 μM to 10.000 μM and a detection limit of 0.185 Mm. More interestingly, citrate-capped Cu NPs have a characteristic absorption peak at 260 nm, we also found that Hg2+ could cause the absorption peak at 260 nm to change. Therefore, we developed another colorimetric method for Hg2+ detection based on the absorption peak of citrate-capped Cu NPs at 260 nm. This colorimetric method was shown to enable convenient and sensitive quantification of Hg2+ in the concentration range of 0.100 μM to 6.000 μM with a limit of detection of 0.052 μM. In this study, a dual-mode sensor for detection of Hg2+ was constructed, which exhibited good sensitivity and selectivity.
研究领域:
分析化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）