JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2019
·
IF 4.029
Daoyao Ke,Anna Jiang,Mattia Biesuz,Mauro Bortolotti,Gianmarco Taveri,Xiaoyi Wang,Ming Xu,Jiang Li,Yagang Feng,Chunfeng Hu,Salvatore Grasso
We developed Cold Isostatic Joining (CIJ) which is an environmental friendly room temperature joining method. This technique extends cold sintering process to joining of glasses. By optimizing the CIJ conditions a shear stress (18 MPa) comparable to bulk fused silica was achieved. The technique surpasses other joining methods (e.g. adhesive bonding and brazing), because it is insensitive to thermal degradation . Unlike pressure-less silicate bonding, pressure assisted CIJ resulted in a thin joining interlayer (≈27 nm) which maintained its integrity after being heated up to 1000 °C. The in-line transmittance (92%) was identical to un-joined material over the full spectrum making the joining nearly undetectable. The mechanism of CIJ formation and joining were clarified using X-ray diffraction (XRD and pole figure), scanning electron microscopy (SEM) and in line transmittance measurements. The cold joining method could find applications in the field of optics and semiconductors for wafer and lens bonding.
研究领域:
材料科学
·
光学工程
·
半导体制造
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连接技术
·
环境友好工艺
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DOI
高影响因子
CERAMICS INTERNATIONAL
·
2018
·
IF 3.057
Li-Xiang Wu,Wei-Ming Guo,Wen-Bin Niu,Rui-Lin Lin,Shi-Kuan Sun,Jia-Xiang Xue,Hua-Tay Lin
Pressureless joining of SiC ceramics at low temperature was developed using Ti powder interlayer. Phase assemblage and microstructure of SiC joint revealed that the products after diffusion bonding mainly consisted of Ti 5 Si 3 and TiC owing to the comparatively low joining temperature. Additionally, Si and C elements were found to be with higher penetration depth in the Ti interlayer. Benefitting from the lower proportion of the brittle Ti 5 Si 3 phase and porosity, shear strength reached a relatively higher value of 41 MPa after joining at 1200 °C for 30 min.
研究领域:
材料科学
·
工程学
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DOI
高影响因子
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
·
2025
·Vol. 72
·期 11
·3233-3243
·
IF 4.5
Kun Qian,Chenguo Yao,Yancheng Wang,Qiang Yang,Sizhe Xiang,Qiying Pei,Ting Zhu,Hongmei Liu,Shoulong Dong
Two major reasons why chemotherapy and immunotherapy have limited efficacy in treating gliomas are the blood-brain barrier and the dense, solid structure of the glioma. Pulsed electric fields have been a powerful tool for ablating solid tumors, and narrowing pulse duration can improve the field homogeneity penetrating into the tumor. In this study, we used multicellular tumor spheroids (MCTSs) as a model to explore the potential of ultrashort nanosecond pulses to inhibit tumor cell activity while disrupting their dense drug-resistant barriers. Exposure to ultrashort pulsed electric fields can significantly inhibit the viability of U-87 MG, C6, and GL261 spheroids, as indicated by reduced intracellular ATP content. Meanwhile, the proliferative abilities of tumor cells were suppressed, as evidenced by reduced Ki67 protein expression. In addition, it is notable that, after exposure to electric fields, the volume of spheroids increased dramatically. We hypothesize that ultrashort pulsed electric fields can reduce tumor compactness, thereby facilitating drug delivery for immunotherapy and chemotherapy. Immunofluorescence results showed that the cell-cell junction was broken by ultrashort pulsed electric fields with lower expression of adherens junction protein N-cadherin and tight junction protein ZO-1. It is evidenced that the capability of ultrashort pulsed electric fields to downregulate the intercellular adherence, as well as suppress the epithelial-mesenchymal transition, a key process of metastasis of cancer cells. At last, aqueous fluorescent nanoparticles were applied to simulate the anticancer drug or therapeutic antibodies. Under the supervision of fluorescence microscopy, the degree of nanoparticles penetrating into the spheroids was positively related to the number of ultrashort pulsed electric fields, marked with a higher fluorescent signal from the inner quiescent zone or a necrotic core. In conclusion, we emphasize that ultrashort pulsed electric fields could be promising for downgrading the compactness of glioma tumors, being a powerful assisted therapy for the delivery of anticancer drugs and therapeutic antibodies.
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DOI
PubMed
高影响因子
Nanotoxicology
·
2021
·
IF 5.913
Chao Wang,Jiansheng Zhu,Xing Gong,Yinyin Liang,Shuyu Xu,Yongquan Yu,Liu Yang,Jiayi Xu,Shou-Lin Wang
This study attempts to explore the potential impact of titanium dioxide nanoparticles (n-TiO2) on bioconcentration and reproductive impairments of male zebrafish in the presence of 2,2′,4,4′-tetrabromodiphenyl ether (BDE47), the congener of PBDEs predominant in environment and most abundant in biosamples. n-TiO2 nanoparticles strongly adsorbed BDE47 to form BDE47/TiO2 complex, which was taken up into the testes of zebrafish, and increased the tissue burdens of both BDE47 and n-TiO2. Correspondingly, no observed toxic dose of n-TiO2 (100 μg/L) was found to aggravate the abnormal histological morphology of the testes and the decrease in egg production, gonadosomatic index, sexual hormone levels and related gene expression in zebrafish in the presence of BDE47 at 5 or 50 μg/L. In addition, n-TiO2 exacerbated the destruction resulting from the ultrastructural disassembly of intercellular connectivity of germ cells in zebrafish and the decrease in transepithelial electrical resistance in TM4 cells induced by BDE47. Furthermore, n-TiO2 enhanced BDE47 to initially activate p-JNK MAPK signaling pathway and subsequently triggered the downregulation of junction proteins (i.e., ZO-1, Connexin-43 and N-cadherin), leading to impaired cell-cell junctions in vivo and in vitro. Our results demonstrated that n-TiO2 should act as a carrier to facilitate the accumulation of BDE47 in zebrafish testes and result in a synergistic effect on BDE47-induced adverse reproductive outcomes via disruption of intercellular connectivity of zebrafish testes. This study is beneficial in providing a scientific basis for improving the health risk assessment of environmental pollutants, particularly those that coexist with nanoparticle contamination in realistic environments.
研究领域:
环境毒理学
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生殖生物学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2021
·Vol. 7
·期 40
·
IF 14.143
Ting Huang,Tianyuan Zhang,Xinchi Jiang,Ai Li,Yuanqin Su,Qiong Bian,Honghui Wu,Ruyi Lin,Ni Li,Hongcui Cao,Daishun Ling,Jinqiang Wang,Yasuhiko Tabata,Zhen Gu,Jianqing Gao
The transfer of mitochondria between cells has recently been revealed as a spontaneous way to protect the injured cells. However, the utilization of this natural transfer process for disease treatment is so far limited by its unsatisfactory transfer efficiency and selectivity. Here, we demonstrate that iron oxide nanoparticles (IONPs) can augment the intercellular mitochondrial transfer from human mesenchymal stem cells (hMSCs) selectively to diseased cells, owing to the enhanced formation of connexin 43–containing gap junctional channels triggered by ionized IONPs. In a mouse model of pulmonary fibrosis, the IONP-engineered hMSCs achieve a remarkable mitigation of fibrotic progression because of the promoted intercellular mitochondrial transfer, with no serious safety issues identified. The present study reports a potential method of using IONPs to enable hMSCs for efficient and safe transfer of mitochondria to diseased cells to restore mitochondrial bioenergetics.
研究领域:
纳米技术
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细胞生物学
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再生医学
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治疗学
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产品关联
DOI
PubMed
高影响因子
Journal of Materiomics
·
2022
·Vol. 8
·期 5
·1001-1008
·
IF 8.589
Xiaoyang Wang,Chun Li,Qihan Zhou,Mingshen Li,Mushi Zheng,Junlei Qi,Xiaoqing Si,Jian Cao
One of the critical challenges for the protonic ceramic fuel cell stack is sealing electrolytes and interconnects. However, the traditional Ag CuO sealant will aggravate the oxidation along the interconnect surface and result in brittle compound layers at the BaZr 0.1 Ce 0.7 Y 0.1 Yb 0.1 O 3- δ (BZCYYb) electrolyte interface. The present work demonstrates that a silver particle interlayer with high dislocation density can be adopted to join BZCYYb electrolyte to the interconnect (AISI 441 stainless) in air instead of traditional Ag CuO sealant. Elevating temperatures result in a sufficient bonding at the Ag/BZCYYb interface, and a defect-free joint is obtained at 950 °C. Atomic bonding at Ag/BZCYYb interface is confirmed by TEM. Also, a dense and thin oxide layer (2–3 μm) is formed along the AISI 441 interface. Ag particles in the interlayer provide the main driving force for the sintering joining. The massive dislocations promote the recovery and recrystallization of the Ag interlayer, as well as the interdiffusion of BZCYYb/Ag. After aging in the wet oxidizing atmosphere at 600 °C for 300 h, joints remain intact and dense, indicating superior oxidation resistance and aging performance. Besides, the joint shear strength (25.3 MPa) is 59 % higher than that of the joint brazed by traditional Ag CuO.
研究领域:
材料科学
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固态离子学
·
能源材料
·
电化学
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连接技术
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高温材料
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DOI
高影响因子
Crystals
·
2021
·Vol. 11
·期 3
·278
·
IF 2.589
Liangqun Yang,Hongli Suo,Lin Ma,Min Liu,Wanli Zhao,Jianhua Liu,Lei Wang,Zili Zhang,Qiuliang Wang
In this study, boron powder with different particle sizes was purified by both chemical and heat treatment methods. The reduction in the particle size can improve the chemical purification with no effort on the heat treatment. The superconducting properties of the powder drastically changed even with only a partial elimination of oxygen. On the one hand, less oxygen content resulted in highTcandJcvalues under the low magnetic field, and most importantly, a significant improvement in the superconducting connectivity (Afvalue). On the other hand, the degradation ofJcunder a high field and a change in the pinning mechanism were also found, along with decreasing oxygen. This result indicated that oxygen, probably MgO, might act as the pinning center and as an obstacle for the supercurrent in MgB2at the same time. This work paves the way for obtaining pure oxygen-free MgB2and understanding the real effect of oxygen in MgB2.Keywords:boron powder;purification;particle size;MgB2;superconducting connectivity
研究领域:
材料科学
·
物理学
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DOI
高影响因子
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
·
2023
·Vol. 64
·期 14
·30-30
·
IF 4.4
Guodong Liu,Le Feng,Xiaoqiang Liu,Peng Gao,Fang Wang
Purpose:This study aimed to investigate the effects of O-linked N-acetylglucosamine modification (O-GlcNAcylation) on connexin43 (Cx43) expression and its subsequent effects on tight junction properties in diabetic retinopathy (DR).Methods:O-GlcNAcylation levels in primary human retinal vascular endothelial cells (HRVECs) and retinas from rats with diabetes were regulated by treatment with Thiamet G or alloxan. Immunoprecipitation was used to examine the relationship between O-GlcNAcylation and Cx43 expression. Stable overexpression and knockdown of Cx43 in HRVECs were achieved using lentivirus constructs; further, their effects on occludin and zonula occluden-1 (ZO-1) expression and tight junction barrier function were determined.Results:O-GlcNAcylation level increased significantly, whereas Cx43 expression decreased in retinas from rats with diabetes and HRVECs cultured under high-glucose conditions. Immunoprecipitation revealed that Cx43 was modified by O-GlcNAcylation and phosphorylation simultaneously. O-GlcNAcylation inhibition negatively regulated both total Cx43 and phosphorylated Cx43 expression, subsequently disrupting tight junction properties. Conversely, Cx43 overexpression reversed the disruption of tight junction properties and downregulated vascular endothelial growth factor expression. Consistently, Cx43 overexpression increased transendothelial electrical resistance values in HRVEC layers.Conclusions:O-GlcNAcylation negatively regulated Cx43 expression, contributing to the disruption of the blood retinal barrier. However, O-GlcNAcylation inhibition and Cx43 overexpression could reverse the tight junction disruption. Therefore, O-GlcNAcylation inhibition is a potential target for avoiding tight junction disruption through the Cx43 pathway in DR.
研究领域:
糖尿病学
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细胞生物学
·
分子生物学
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眼科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 2
·366
·
IF 4.2
Zhuojian Lu,Rui Ge,Bin Zheng,Peng Zheng
Antibody arrays play a pivotal role in the detection and quantification of biomolecules, with their effectiveness largely dependent on efficient protein immobilization. Traditional methods often use heterobifunctional cross-linking reagents for attaching functional residues in proteins to corresponding chemical groups on the substrate surface. However, this method does not control the antibody’s anchoring point and orientation, potentially leading to reduced binding efficiency and overall performance. Another method using anti-antibodies as intermediate molecules to control the orientation can be used but it demonstrates lower efficiency. Here, we demonstrate a site-specific protein immobilization strategy utilizingOaAEP1 (asparaginyl endopeptidase) for building a nanobody array. Moreover, we used a nanobody-targeting enhanced green fluorescent protein (eGFP) as the model system to validate the protein immobilization method for building a nanobody array. Finally, by rapidly enriching eGFP, this method further highlights its potential for rapid diagnostic applications. This approach, characterized by its simplicity, high efficiency, and specificity, offers an advancement in the development of surface-modified protein arrays. It promises to enhance the sensitivity and accuracy of biomolecule detection, paving the way for broader applications in various research and diagnostic fields.Keywords:protein immobilization;enzymatic ligation;OaAEP1;nanobody
研究领域:
生物化学
·
分子生物学
·
诊断学
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产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 18
·6129-6137
·
IF 4
Peipei Wang,Jin Tong,Cong Meng,Qing Yuan,Wei Deng,Shu-Yan Yu,Hong-Wei Ma
Owing to their structural designability and tuneable properties, supramolecular metal–organic complexes have recently emerged as promising candidates for the sensing and detection of molecules and anions. Herein, we synthesised three tripyrazolate-linked [M6L2] metallocages with the formulas [(bpyPd)6L2](NO3)6 (1), [(dmbpyPd)6L2](NO3)6 (2), and [(phenPd)6L2](NO3)6 (3) (H3L = tris(4-(5-(trifluoromethyl)-1H-pyrazol-3-yl)phenyl)amine, bpy = 2,2′-bipyridine, dmbpy = 4,4′-dimethylbipyridine, phen = 1,10-phenanthroline). Crystallography revealed that metal-directed coordination and the bidentate chelate behaviour of the ligand induced the self-assembly of supramolecular metal–organic cages. Notably, these cages were employed as turn-on fluorescence sensors for SO2 and its derivative (HSO3−) through a disassembly mechanism. Cages 1, 2, and 3 showed a highly selective and sensitive detection of HSO3− over other common anions in aqueous solutions and of SO2 gas over other common gasses, with an excellent anti-interference ability. These metallocages were subsequently applied as sensors in environmental and biological samples. This study not only enriches the ongoing research on metal–organic supramolecular materials but also facilitates the future preparation of stimuli-responsive supramolecular coordination complexes.
研究领域:
超分子化学
·
分析化学
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DOI
PubMed
高影响因子
Cells
·
2021
·Vol. 10
·期 2
·237
·
IF 6.6
Guobin Li,Lan Zhang,Kaiting Ning,Baoqiang Yang,Francisca M. Acosta,Peng Shang,Jean X. Jiang,Huiyun Xu
Bone–muscle crosstalk plays an important role in skeletal biomechanical function, the progression of numerous pathological conditions, and the modulation of local and distant cellular environments. Previous work has revealed that the deletion of connexin (Cx) 43 in osteoblasts, and consequently, osteocytes, indirectly compromises skeletal muscle formation and function. However, the respective roles of Cx43-formed gap junction channels (GJs) and hemichannels (HCs) in the bone–muscle crosstalk are poorly understood. To this end, we used two Cx43 osteocyte-specific transgenic mouse models expressing dominant negative mutants, Δ130–136 (GJs and HCs functions are inhibited), and R76W (only GJs function is blocked), to determine the effect of these two types of Cx43 channels on neighboring skeletal muscle. Blockage of osteocyte Cx43 GJs and HCs in Δ130–136 mice decreased fast-twitch muscle mass with reduced muscle protein synthesis and increased muscle protein degradation. Both R76W and Δ130–136 mice exhibited decreased muscle contractile force accompanied by a fast-to-slow fiber transition in typically fast-twitch muscles. In vitro results further showed that myotube formation of C2C12 myoblasts was inhibited after treatment with the primary osteocyte conditioned media (PO CM) from R76W and Δ130–136 mice. Additionally, prostaglandin E2 (PGE2) level was significantly reduced in both the circulation and PO CM of the transgenic mice. Interestingly, the injection of PGE2 to the transgenic mice rescued fast-twitch muscle mass and function; however, this had little effect on protein synthesis and degradation. These findings indicate a channel-specific response: inhibition of osteocytic Cx43 HCs decreases fast-twitch skeletal muscle mass alongside reduced protein synthesis and increased protein degradation. In contrast, blockage of Cx43 GJs results in decreased fast-twitch skeletal muscle contractile force and myogenesis, with PGE2 partially accounting for the measured differences.Keywords:osteocytes;Cx43;gap junctions;hemichannels;bone–muscle crosstalk
研究领域:
生物力学
·
分子生物学
·
生理学
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DOI
PubMed
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
·
2019
·Vol. 234
·期 10
·17999-18016
·
IF 4.522
Zhida Shen,Qi Chen,Tingting Jin,Meihui Wang,Hangying Ying,Jiangting Lu,Ming Wang,Wenbin Zhang,Fuyu Qiu,Chongying Jin,Yanbo Zhao,Guosheng Fu
Theaflavin 3,3′-digallate (TF3), is reported to protect cardiomyocytes from lipotoxicity and reperfusion injury. However, the role of TF3 in the protection of high-glucose injury is still poorly understood. This study investigated the protective effects of TF3 on gap junctions and autophagy in neonatal cardiomyocytes (NRCMs). NRCMs preincubated with high glucose were coincubated with TF3. The expression of connexins and autophagy-related proteins was determined. The functioning of gap-junctional intercellular communication (GJIC) was measured by a dye transfer assay. Adenosine monophosphate-activated protein kinase (AMPK) activity was determined by western blot. Moreover, AMPK was activated with aminoimidazole-4-carboxamide-1-β- d -ribofuranoside (AICAR) or inhibited by AMPKα small interfering RNA (siRNA) to explore the role of AMPK in the modulation of connexin 43 (Cx43) and autophagy. Meanwhile, autophagy was activated or blocked to observe the change in Cx43 expression. It was found that the protein expression of Cx43 and autophagy-related proteins was increased in a TF3 dose- and time-dependent manner under high glucose. TF3 also recovered the reduced GJIC function induced by high glucose concentrations. TF3 activated phosphorylated AMPK in a time-dependent way. AMPKα siRNA abrogated the protection of TF3, while AICAR showed similar results compared to the TF3 treatment. Meanwhile, autophagy activation caused decreased Cx43, while cotreatment with baf A1 enhanced Cx43 expression further compared with the TF3 treatment alone under high glucose. We concluded that TF3 partly reversed the inhibition of Cx43 expression and autophagy induced by high glucose in NRCMs, partly by restoring AMPK activity. Inhibition of autophagy might be protective by preserving Cx43 expression in NRCMs stimulated by high glucose.
研究领域:
心脏病学
·
分子生物学
·
细胞生物学
·
药理学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）