Marine Drugs
·
2021
·Vol. 19
·期 11
·596
·
IF 5.118
Lin-Lin Zhang,Xiao-Hua Jiang,Xin-Feng Xiao,Wen-Xiu Zhang,Yi-Qian Shi,Zhi-Peng Wang,Hai-Xiang Zhou
(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacteriumRenibacteriumsp. Y82 was expressed inEscherichia coli, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni–NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0–50 °C), and with optimal activity at 40 °C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20–30 °C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn2+and Co2+. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (w/v) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS.Keywords:chitosanase;cold adaptation;chitooligosaccharide;Renibacteriumsp.
研究领域:
生物化学
·
生物技术
·
海洋生物学
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DOI
PubMed
高影响因子
Nano Research
·
2023
·Vol. 17
·期 4
·3120-3129
·
IF 9.9
Hu Lulin,Yu Xinxin,Hao Jingcheng,Xu Lu
Owing to their low flexibility, poor processability and a lack of responsiveness, inorganic materials are usually non-ideal for constructing a living organism. Hence, to date, lifelike materials with structural hierarchies and adaptive properties usually rely on light and soft organic molecules, although few exceptions have been acquired using two-dimensional (2D) inorganic nanosheets. Herein, with a systematic study on the gelation behavior of carbon-based 0D quantum dots, 1D nanotubes, and 3D fullerenes, we find that acidified 1D carbon nanotubes (CNTs) can serve as an alternative building block for fabricating purely inorganic biomimetic soft materials. The as-prepared CNT gels exhibit not only a pH- or photothermal-triggered mechanical and tribological adaptivity, which allows them to simulate the behavior of sea cucumbers, peacock mantis shrimps, and mammalian muscles or cortical bones, but also a unique damping property that is similar to spider’s cuticular pad. Their high elasticity, effective lubrication, excellent biocompatibility, and controllable friction and wear also allow them to function as a new type of smart lubricants, whose tribological properties can be regulated either by its internal pH changes or spatiotemporally by near-infrared (NIR) light irradiations, free of any toxic and flammable base oils or additives.
研究领域:
材料科学
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纳米技术
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柔性机器人
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仿生学
·
摩擦学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Min Mao,Qiangli Zhao,Jiahao Kang,Qiang Mu,Jie Tao,Xiaoliang Zhao,Xinhai He,Jianwei Li
Green cellulose-based triboelectric materials are increasingly in demand for self-powered flexible sensors and energy harvesting applications. However, cellulose exhibits low polarity, limited surface charge generation capacity, weak positive triboelectric properties, and susceptibility to excessive hydration in high-humidity environments, leading to poor structural stability, charge dissipation, and consequently reduced triboelectric performance. In this study, microcrystalline cellulose was utilized as the matrix, while polyethyleneimine and Fe 3+ were incorporated with ethanol fumigation to construct recyclable, biodegradable, and biocompatible composite films. These films feature multi-crosslinked structures and abundant surface/interface morphologies. Ethanol fumigation enhanced the triboelectric output voltage of the film-based TENG by 198 % (248.3 V). The TENG demonstrated exceptional pressure sensitivity (3.136 V/N), high-humidity sensing capability (80–98 % RH, 3.8 V/RH), humidity adaptability, and fatigue resistance (98 % RH, 21,000 cycles). The TENG could simultaneously power up to 500 LEDs and shows potential applications in human health monitoring and intelligent recognition. This study proposes a convenient and scalable strategy to enhance both the triboelectric output and humidity adaptability of cellulose-based triboelectric materials, effectively expanding their practical applications.
研究领域:
材料科学
·
能源技术
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DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Zhen Qin,Qiming Chen,Si Lin,Sa Luo,Yongjun Qiu,Liming Zhao
Chitooligosaccharide is widely used as a functional food additive and a valuable pharmacological agent. The transformation of chitinous biomass into valuable bioactive chitooligosaccharides is one of the most exciting applications of chitosanase. A novel glycoside hydrolase (GH) family 46 chitosanase (GsCsn46A) from rhizobacterium Gynuella sunshinyii was cloned and heterologously expressed in Escherichia coli . GsCsn46A showed maximal activity at pH 5.5 and 30 °C. GsCsn46A featured remarkable cold-adapted property, which controllably hydrolyzed chitosan to three types of chitooligosaccharides at the mild reaction condition (reaction condition: pH 5.5 at 30 °C; method for stopping the reaction: 50 °C for 30 min). The yields of three types of chitooligosaccharides products (degree of polymerization (DP): 2–7, 2–5 and 2–3) were 70.9%, 87.1% and 94.6% respectively. This novel cold-adapted chitosanase provides a cleaner production process for the controllable preparation of chitooligosaccharides with the specific DP.
研究领域:
生物化学
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酶工程
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食品科学
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DOI
PubMed
高影响因子
Results in Engineering
·
2025
·
IF 7.9
Wei Kuang,Yuxiang Hong,Zimu Hu,Peng Xu
Liquid C1 feedstock, including methanol, formaldehyde and formate can be easily manufactured from greenhouse gases CO 2 and CH 4 . Yarrowia lipolytica , isolated from marine ecosystem, is a non-conventional yeast which is capable of assimilating multiple range of hydrophobic compounds including alcohols, lipids, hydrocarbons, and volatile fatty acids. This yeast has been extensively engineered as a benchmark host to produce lipids, resveratrol, carotenoids, squalene and other natural products. Connecting C1 feedstock pathways to these important metabolites holds promise to further reduce costs and improve sustainability. Herein we tested the growth fitness of Y. lipolytica when exposed to various one-carbon (C1) liquid feedstocks (methanol, formaldehyde, and formate). We firstly examined the cell growth kinetics with an automatic Cell Growth Analyzer and fitted the data with a Monod-type growth model. We obtained the inhibition constants of the three liquid C1s from the growth fitness dose-response curves. We found that the lagging phase duration is exponentially correlated with the toxicity of the tested C1 solvents. Notably, Y. lipolytica exhibited tolerance to methanol (IC 50 871 mM) and formate (IC 50 42.6 mM), but showed sensitivity to formaldehyde (IC 50 3.8 mM). We further demonstrated that glucose and formate co-substrate could improve cell growth and lipid production. This work broadens Y. lipolytica ’s substrate scope and facilitates sustainable and cost-efficient production of fuels and chemicals from C1 feedstocks.
研究领域:
微生物学
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生物工程
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环境科学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Tao Li,Lisha Yuan,Dun Jiang,Shanchun Yan
Strong multi-host adaptability significantly contributes to the rapid dissemination of Hyphantria cunea. The present study explores the involvement of cytochrome P450 monooxygenase (P450) in the multi-host adaptation of H. cunea and aims to develop RNA pesticides targeting essential P450 genes to disrupt this adaptability. The results showed that inhibiting P450 activity notably reduced larval weight and food-intake across seven plants groups. The P450 gene HcCYP6AE178 was highly upregulated in H. cunea larvae from medium- and low-preference host plant groups. Silencing HcCYP6AE178 significantly decreased H. cunea larval body weight, increased larval mortality, inhibited energy metabolism genes expression and interfered with growth regulatory genes expression. Overexpression of HcCYP6AE178 enhanced the tolerance of Drosophila and Sf9 cells to the plant defensive substances cytisine and coumarin. The RNA pesticide CS-ds HcCYP6AE178 constructed using chitosan (CS) exhibited remarkable stability. Treatment with CS-ds HcCYP6AE178 effectively reduced H. cunea larval body weight, heightened larval mortality, and disrupted growth regulatory genes expression in low-preference host plant groups. Combined treatment of CS-ds HcCYP6AE178 and coumarin significantly elevated H. cunea larval mortality compared to coumarin alone, accompanied by the inhibition of growth regulatory genes expression and an abnormal increase in energy metabolism genes expression. Taken together, HcCYP6AE178 is essential for the adaptation of H. cunea to multiple host plants, and RNA pesticides targeting HcCYP6AE178 can effectively impair the performance of H. cunea in different host plants.
研究领域:
昆虫学
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分子生物学
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植物保护
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DOI
高影响因子
JOURNAL OF ANTIBIOTICS
·
2025
·
IF 2.7
Jiang Hanxiang,Qi Jiangfeng,Wang Jiwen,Chen Jiaqin,Feng Dong,Yang Junbiao,Liu Xinna,Liu Mengqun,Zhou Xvzhe,An Zhilong,Lu Yuanyuan,Ge Chun,Wang Ying
Acetobacter baumannii poses escalating clinical challenges due to its exceptional adaptability, demanding innovative antimicrobial strategies. This study pioneers an investigation into the antibacterial efficacy and molecular mechanism of Terramide A, a hydroxamate siderophore isolated from Aspergillus terreus, against notorious A. baumannii . Employing a multidisciplinary approach integrating phenotypic characterization with mechanistic interrogation, we demonstrate that Terramide A exerts significant inhibitory effects against A. baumannii and P. aeruginosa , pathogens critically dependent on siderophore-mediated iron acquisition for survival and virulence. Structural characterization underlines the hydroxamate moieties of Terramide A presumably supports its hypothesized role as a fungal siderophore, involving competitive iron sequestration and bacterial homeostasis. Subsequently, multi-omics investigation of susceptible strain AB19606 delineated a metabolic collapse cascade due to iron acquisition competition: (1) impairment of central metabolism and energy production through oxidative phosphorylation (OXPHO) inhibitions; (2) compromised stress adaptation and bacterial flexibility; (3) compensatory overactivation of siderophores biosynthesis and transportation, depleting metabolic intermediates and exacerbating stress; (4) coordinated suppression of virulence determinants, such as secretory systems and biofilm formation. These molecular derangements translated into phenotypic deficits, including quorum sensing, diminished autoinducer peptides production, and morphological/functional abnormalities. In vivo evaluation in a rat skin wound infection model further demonstrated that Terramide A promotes wound healing and mitigates inflammation, supporting its antibacterial efficacy. These findings establish Terramide A as a promising antibacterial agent and provide critical insights into iron-competitive antimicrobial strategies to exploit micro-nutrient deprivation and metabolic dysfunction. However, further research is needed to optimize the siderophore-based scaffold, clarify its mechanisms, and assess therapeutic potential.
研究领域:
微生物学
·
药理学
·
感染病学
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DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Li Chun-Bo,Wang Fang,Wang Yi-Yang,Guo Mei-Lin,Li Jin-Le,Deng Cong,Song Fei,Yang Wei,Wang Yu-Zhong
Superamphiphobic and flame-retardant fabrics offer effective protection for firefighters and industrial workers operating under hazardous conditions. However, limitations in deformation resistance, wear comfort, and environmental adaptability hinder their practical applications. Here, a monolithic hierarchical macro-/micro-/nanostructure is constructed to achieve durable repellency against water and oils, even under significant deformations. This coating integrates fluorinated nanoparticles, flame retardant microparticles, and a cross-linking adhesive. Hydrogen bonding and the adhesive define the coating’s morphology, robustness, and adaptability. The coated surface exhibits an ultralow water adhesion force (0.002 mN) and excellent anti-fouling performance against extreme temperatures (100 °C, −196 °C) and corrosive liquids, including aqua regia and concentrated H 2 SO 4 . Upon fire exposure, the coating enables self-extinguishing behavior on cotton fabrics. The coated fabrics also demonstrate remarkable mechanical and UV resistance while preserving wear comfort. Overall, we achieve a balance between desirable properties and wear comfort in superamphiphobic, flame-retardant fabrics, enabling protective clothing applications previously unattainable.
研究领域:
材料科学
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表面工程
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纺织科学
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产品关联
DOI
PubMed
高影响因子
Geoenergy Science and Engineering
·
2023
·
IF 0
Wenchuan Wu,Xiaorong Yu,Anbang Hu,Han Yan,Zhengliang Dong,Huan Yang,Gaoshen Su
Amphoteric retarder (AIDA) was prepared by radical copolymerization of 2-acrylamido-2-methyl propane sulfonic acid (AMPS), itaconic acid (IA), 2-(Methacryloyloxy) ethyltrimethylammonium chloride (DMC) and methyl allyl polyethenoxy ether (TPEG). The characteristics of AIDA were verified by FTIR and 1 HNMR. In addition, the performances of AIDA in cement slurry including thickening time, compressive strength developments, and adaptability to long standing cementing with lager temperature differences were investigated. The results indicated that AIDA possessed excellent temperature-resistance (180 °C) and good adaptability to long standing cementing with larger temperature differences of 90 °C. Finally, the retarding mechanism of AIDA was revealed through transverse relaxation time (T 2 ) distribution, XRD tests, and adsorption behavior of AIDA on cement particles. The results demonstrated that the stronger retarding effect of AIDA originated from the dual adsorption of AIDA on cement particles compared with AMPS-IA copolymer (PAI). AIDA outperformed PAI in its adaptability to long-term cementing, which was responsible to (a): the adsorbed amount of AIDA at higher temperature is higher than that of lower temperatures, therefore, AIDA has a stronger retarding effect at higher temperature and weaker retarding effect at lower temperature. (b) Compared to PAI, the adsorption strength of AIDA is weaker and easy to de-adsorb, so both the retarding effect and the hydration reaction of cement are realized. Thus, the super-retarding phenomenon of cement slurry containing AIDA is effectively prevented.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jingxuan Zhao,Ying Xue,Yue Wu,Xiaonan Hao,Shulin Sun,Huiliang Zhang
This study presents an innovative approach to membrane fabrication by integrating electrospinning with surface modification techniques to develop a novel dual-layer protective membrane system. The key innovation lies in the incorporation of photothermally responsive nanocomposites, enabling multifunctional membrane performance. The primary layer is a PVDF-PNIPAAm thermoresponsive matrix synthesized by radical polymerization, integrated with BN@PDA-Ag nanosheets. This design combines Ag nanoparticles’ photothermal conversion and BN’s thermal conductivity to regulate pore size via PNIPAAm chain conformational changes induced by temperature. The secondary layer, comprising CS@CNT and PVDF, significantly enhances membrane hydrophilicity and antifouling properties through chitosan’s hydrophilic groups and chelating capabilities. The membrane demonstrates self-cleaning, adaptive filtration, antifouling and high flux, representing a significant advancement in smart membrane technology for water treatment applications.
研究领域:
材料科学
·
环境工程
·
纳米技术
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Leyi Lin,Zhiping Su,Haishan Zhang,Guowen Zhou,Haonan Zhou,Junli Ren,Xiaoying Wang,Chuanfu Liu,Xiaohui Wang
The increasing environmental burden generated by disposable plastic wastes makes the development of sustainable substitute materials an emergent task. As one of the most abundant bioresources, chitosan (CS) has been considered as a potential candidate for plastic substitution. Conventionally, CS-based materials are fabricated through a solution-processing procedure due to the high crystallinity of CS. Herein, we designed a CS-based material via integrating CS into the network of polyimine (PI), which shows thermomechanical processability similar to plastics. Strong interactions were achieved through dynamic imine bond and hydrogen bond and thus formed a thermo-processable dynamic composite network. These CS-based plastic substitutes exhibit exceptional mechanical performances, excellent thermal/chemical stability, and a series of self-adaptiveness, including re-healing, reprocessing and multi-layer laminating. Notably, CPs can be easily degraded and 100% recycled for the production of next-generation materials. This work provides an alternative route to produce green and sustainable biomass materials as a plastic substitute.
研究领域:
材料科学
·
生物工程
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2022
·Vol. 101
·期 6
·3262-3272
·
IF 2.5
Dongmei Jia,Jia Guo,Jing Li,Yongzheng Duan
The composite of CuO coated MIL-101(Fe) (CuO@MIL-101(Fe)) was successfully prepared using a one-pot hydrothermal technology. X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), Brunauer–Emmett–Teller (BET), and TG technologies were used to characterize its structural, morphological, and physicochemical properties. CuO@MIL-101(Fe) exhibited a better pH adaptivity, a faster removal ratio of 95.8% for glyphosate within 10 min at pH 7, and a higher selectivity of adsorption capacity, even reaching 231.6 and 233.5 mg g −1 with coexisting Cl − (10 mmol L −1 ) and SO 4 2− (10 mmol L −1 ). The enhanced adsorption performances of CuO@MIL-101(Fe) may be caused by Cu forming a coordination interaction with glyphosate. A possible mechanism of glyphosate removal was discussed on the basis of the FTIR and X-ray photoelectron spectroscopy (XPS) analysis. CuO@MIL-101(Fe) composite would be more favourable to capture glyphosate from wastewater.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）